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The map label for this gene is miaA

Identifier: 150397212

GI number: 150397212

Start: 2062086

End: 2063003

Strand: Reverse

Name: miaA

Synonym: Smed_2011

Alternate gene names: 150397212

Gene position: 2063003-2062086 (Counterclockwise)

Preceding gene: 150397215

Following gene: 150397209

Centisome position: 54.55

GC content: 63.62

Gene sequence:

>918_bases
ATGCAAAACCTTGCGAGAGACATCGACGCGATCCTGATAACCGGACCAACCGCCAGCGGCAAGTCTGCGCTGGGGGTGAA
GCTTGCGCAGAGACACGGGGGCGTCGTGATCAATGCCGATAGCATGCAGGTTTACGGCACGCTGAAAATACTGACCGCGC
GCCCGGACGAAAGCGAGATGGGGGGAGTCGAGCACTTCCTTTACGGCCACGTGCCGCCGGACCGTGCCTATTCAACCGGC
GCATGGCTGCGCGAGGCGGAAGCGCTGGTGGCGAGATTGAGGATAGAGGGGCGGATGCCCGTTTTCGTCGGCGGCACCGG
CCTTTATTTCAAGGCGCTGACGGGAGGGCTTTCCGACATGCCGGAGGTTCCGCATGCGATCCGCCAGCGCCTGCGGAAGC
GCCTGATGGAGGAGGGTGCGGAGGCGCTCCACTACGAATTATCCGCTCTGGATCCGGAAACGGCGCAGCGCGTGCGATCC
GGTGACGGGCAAAGGATCGTGCGCGCGCTGGAGGTGATGGAGGCGACTGGAAGGCCGATCGGATTTTACCAACAGAGCCG
AGGTCCTGTCATCATCGATGCCACTCGGGCGCGGAAGATCGTCGTCCTGCCTGAGCGGCCGGTGCTTCATAGCCGCATCA
ATCGGCGCTTCGAGGCAATGCTTGGCATGGGGGCCGTCGAAGAGGTGCGCGCGCTTCTCGCGCTTGATCTTCCGCTGGAG
ATGCCGGCGATGAAGGCGATCGGCGTGCAGCAGATCGCCGCGATGCTGAAAGGCGAGATGAGCGAGGCGCAGGCGATCGA
GGCCGGCGCGGCTCAGACGCGGCAATATGCGAAGCGCCAGATGACCTGGTTTCGCAACCAGCTCGACGAGACCTGGCAGC
GTATCGACGCTGCGGAAGCGCTGGGCGAAAACCATTGA

Upstream 100 bases:

>100_bases
GAGTCCTGATGCATCGAGGCGTTCCGCTGCCGCCTCCGCGCGTGCTGGCGTCAGAACCGGATTTGACGGATTGGCGATAA
GCGTGGCAACGAGAGCCATG

Downstream 100 bases:

>100_bases
ACAGGCGCCCGTCCGGCCTCCATGAGGCGAAGAGCGCGCAAGTATCGGCGGCTCTAACGGGTGCCGTGATCGACCGGCGT
CTGCTGGTGCTGGATGAGCT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MQNLARDIDAILITGPTASGKSALGVKLAQRHGGVVINADSMQVYGTLKILTARPDESEMGGVEHFLYGHVPPDRAYSTG
AWLREAEALVARLRIEGRMPVFVGGTGLYFKALTGGLSDMPEVPHAIRQRLRKRLMEEGAEALHYELSALDPETAQRVRS
GDGQRIVRALEVMEATGRPIGFYQQSRGPVIIDATRARKIVVLPERPVLHSRINRRFEAMLGMGAVEEVRALLALDLPLE
MPAMKAIGVQQIAAMLKGEMSEAQAIEAGAAQTRQYAKRQMTWFRNQLDETWQRIDAAEALGENH

Sequences:

>Translated_305_residues
MQNLARDIDAILITGPTASGKSALGVKLAQRHGGVVINADSMQVYGTLKILTARPDESEMGGVEHFLYGHVPPDRAYSTG
AWLREAEALVARLRIEGRMPVFVGGTGLYFKALTGGLSDMPEVPHAIRQRLRKRLMEEGAEALHYELSALDPETAQRVRS
GDGQRIVRALEVMEATGRPIGFYQQSRGPVIIDATRARKIVVLPERPVLHSRINRRFEAMLGMGAVEEVRALLALDLPLE
MPAMKAIGVQQIAAMLKGEMSEAQAIEAGAAQTRQYAKRQMTWFRNQLDETWQRIDAAEALGENH
>Mature_305_residues
MQNLARDIDAILITGPTASGKSALGVKLAQRHGGVVINADSMQVYGTLKILTARPDESEMGGVEHFLYGHVPPDRAYSTG
AWLREAEALVARLRIEGRMPVFVGGTGLYFKALTGGLSDMPEVPHAIRQRLRKRLMEEGAEALHYELSALDPETAQRVRS
GDGQRIVRALEVMEATGRPIGFYQQSRGPVIIDATRARKIVVLPERPVLHSRINRRFEAMLGMGAVEEVRALLALDLPLE
MPAMKAIGVQQIAAMLKGEMSEAQAIEAGAAQTRQYAKRQMTWFRNQLDETWQRIDAAEALGENH

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=318, Percent_Identity=26.7295597484277, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1790613, Length=278, Percent_Identity=38.8489208633094, Blast_Score=186, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI25144712, Length=299, Percent_Identity=25.752508361204, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324848, Length=300, Percent_Identity=27.3333333333333, Blast_Score=122, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24649830, Length=337, Percent_Identity=25.5192878338279, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_SINMW (A6UB14)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001327679.1
- ProteinModelPortal:   A6UB14
- SMR:   A6UB14
- STRING:   A6UB14
- GeneID:   5322870
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_2011
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   SMED366394:SMED_2011-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33614; Mature: 33614

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNLARDIDAILITGPTASGKSALGVKLAQRHGGVVINADSMQVYGTLKILTARPDESEM
CCCHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEECCCEEEEEEEEEEEECCCCHHH
GGVEHFLYGHVPPDRAYSTGAWLREAEALVARLRIEGRMPVFVGGTGLYFKALTGGLSDM
CCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCC
PEVPHAIRQRLRKRLMEEGAEALHYELSALDPETAQRVRSGDGQRIVRALEVMEATGRPI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
GFYQQSRGPVIIDATRARKIVVLPERPVLHSRINRRFEAMLGMGAVEEVRALLALDLPLE
CCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
MPAMKAIGVQQIAAMLKGEMSEAQAIEAGAAQTRQYAKRQMTWFRNQLDETWQRIDAAEA
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGENH
HCCCC
>Mature Secondary Structure
MQNLARDIDAILITGPTASGKSALGVKLAQRHGGVVINADSMQVYGTLKILTARPDESEM
CCCHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEECCCEEEEEEEEEEEECCCCHHH
GGVEHFLYGHVPPDRAYSTGAWLREAEALVARLRIEGRMPVFVGGTGLYFKALTGGLSDM
CCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCC
PEVPHAIRQRLRKRLMEEGAEALHYELSALDPETAQRVRSGDGQRIVRALEVMEATGRPI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
GFYQQSRGPVIIDATRARKIVVLPERPVLHSRINRRFEAMLGMGAVEEVRALLALDLPLE
CCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
MPAMKAIGVQQIAAMLKGEMSEAQAIEAGAAQTRQYAKRQMTWFRNQLDETWQRIDAAEA
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGENH
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA