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The map label for this gene is ilvI [H]

Identifier: 150397209

GI number: 150397209

Start: 2057495

End: 2059273

Strand: Reverse

Name: ilvI [H]

Synonym: Smed_2008

Alternate gene names: 150397209

Gene position: 2059273-2057495 (Counterclockwise)

Preceding gene: 150397212

Following gene: 150397208

Centisome position: 54.45

GC content: 61.21

Gene sequence:

>1779_bases
ATGAGCGGAACTGAGAACCAGATGACGGGCGCGGAGATCGTTCTCCAGGCTTTGAAAGACAATGGCGTCGAACACATCTT
CGGCTATCCCGGCGGCGCCGTGCTTCCAATCTACGACGAGATTTTCCAGCAGGAGGACATACAGCACATCCTCGTCCGCC
ACGAGCAGGGCGCCGGCCACATGGCCGAAGGGTATGCCCGCTCCACCGGCAAGGTCGGCGTCATGCTGGTGACATCCGGC
CCGGGGGCGACCAATGCGGTTACCCCGCTGCAGGACGCGCTGATGGACTCCATCCCTCTCGTCTGCATCTCGGGGCAGGT
ACCGACATCGCTGATCGGTTCTGACGCCTTCCAGGAGTGCGACACGGTCGGAATTACGCGGCCTTGCACCAAGCACAACT
GGCTGGTCAGGGACGTCAACGAACTCGCCCGCGTCATCCACGAAGCTTTCCGCGTCGCCCAGTCGGGCCGCCCCGGACCG
GTCGTCGTCGACATTCCGAAGGACATCCAGTTTGCGACCGGTGCCTATACGCCGCCTTCGGCCGTTCCAACGCAGAAGAG
CTATCAGCCGAAAACCCAGGGCGACCTGAAGAAGATCGAGGAGGCGGTTGCGCTCATGAAGTCCGCGCGCCAGCCGGTTA
TCTATTCGGGCGGCGGCGTCATCAATTCCGGCCCGCAAGCCGCACATTTCCTGCGCGAGCTGGTCGAACTCACCGATTTT
CCGATCACGTCGACGCTGATGGGCCTCGGCGCCTATCCTGCCTCCGGCAAGAACTGGCTTGGCATGCTCGGCATGCATGG
AACCTATGAAGCCAATCTCGCCATGCACGATTGTGACGTCATGATCTGCATAGGAGCGCGCTTTGACGACCGTATCACCG
GCCGGCTCAATGCCTTCTCGCCGAATTCAAAGAAGATCCACATCGATATCGACCCGTCCTCGATCAACAAGAACGTCCGT
GTCGACGTTCCGATCATCGGCGACGTCGCCGCAGTCCTCGAGGACATGGTTCGCCTGTGGCGTGCTGCGGCCAAGACCGT
CGACCGGACGCGGCTCGAGGATTGGTGGAAATCGATCACCAAGTGGCGCGCGCGCAATTCGCTCGCCTATACGCCGAGTG
CCGATGTCATCATGCCGCAATATGCGATCCAGCGGCTTTACGAGCTCACCAAGGACCGCGACACCTACATCACCACCGAA
GTGGGACAGCACCAGATGTGGGCGGCGCAGTTCTTCGGATTCGAGGAGCCGAACCGGTGGATGACGTCGGGCGGCCTCGG
CACCATGGGCTACGGCTTCCCGGCCGCCGTGGGCGTTCAGGTTGCGCATCCGGATAGCCTCGTCATCGATATCGCGGGCG
ACGCCTCGATCCAGATGTGCATCCAGGAAATGTCCTGCGCGGTTCAGTACGGCCTGCCGGTCAAGATCTTCATCCTGAAC
AATCAATATATGGGCATGGTCCGGCAGTGGCAGCAACTGCTCCACGGCAACCGACTGTCGCATTCCTATACCGAGGCGAT
GCCTGACTTCGTCAAGCTCGCCGAGGCCTATGGCGGCGTTGGCATCCGTTGCGAAAAGCCGGGAGAGCTCGACGAAGCAA
TCAAGCAGATGATCGATACTCCGGCTCCGGTCATTTTCGATTGCCGAGTCGCAAATCTCGCCAATTGCTTCCCGATGATC
CCCTCGGGCAAGGCGCATAACGAAATGCTGCTCCCCGACGAGGCCACGGACGAGGCGGTTGCCAACGCCATCGACGCCAA
GGGCCGCCAGCTCGTTTGA

Upstream 100 bases:

>100_bases
GTAGCCACCCATGCGCTGAACGAGGCTCCTGACGGGGCCTTTTTTTATGCCTTCAAACACGTTATTCACCCAAACCTGAA
CGAGAGACGGAAGCAGGCCA

Downstream 100 bases:

>100_bases
GGACAAGGGTAGGAACAGGATCATGAACGCACATCTTCAGCCTACGGGCTCGGCCTACTTCATCGCCAAGGAAACCGAGC
TTGCGGAAACGCACACGCTA

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MSGTENQMTGAEIVLQALKDNGVEHIFGYPGGAVLPIYDEIFQQEDIQHILVRHEQGAGHMAEGYARSTGKVGVMLVTSG
PGATNAVTPLQDALMDSIPLVCISGQVPTSLIGSDAFQECDTVGITRPCTKHNWLVRDVNELARVIHEAFRVAQSGRPGP
VVVDIPKDIQFATGAYTPPSAVPTQKSYQPKTQGDLKKIEEAVALMKSARQPVIYSGGGVINSGPQAAHFLRELVELTDF
PITSTLMGLGAYPASGKNWLGMLGMHGTYEANLAMHDCDVMICIGARFDDRITGRLNAFSPNSKKIHIDIDPSSINKNVR
VDVPIIGDVAAVLEDMVRLWRAAAKTVDRTRLEDWWKSITKWRARNSLAYTPSADVIMPQYAIQRLYELTKDRDTYITTE
VGQHQMWAAQFFGFEEPNRWMTSGGLGTMGYGFPAAVGVQVAHPDSLVIDIAGDASIQMCIQEMSCAVQYGLPVKIFILN
NQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGVGIRCEKPGELDEAIKQMIDTPAPVIFDCRVANLANCFPMI
PSGKAHNEMLLPDEATDEAVANAIDAKGRQLV

Sequences:

>Translated_592_residues
MSGTENQMTGAEIVLQALKDNGVEHIFGYPGGAVLPIYDEIFQQEDIQHILVRHEQGAGHMAEGYARSTGKVGVMLVTSG
PGATNAVTPLQDALMDSIPLVCISGQVPTSLIGSDAFQECDTVGITRPCTKHNWLVRDVNELARVIHEAFRVAQSGRPGP
VVVDIPKDIQFATGAYTPPSAVPTQKSYQPKTQGDLKKIEEAVALMKSARQPVIYSGGGVINSGPQAAHFLRELVELTDF
PITSTLMGLGAYPASGKNWLGMLGMHGTYEANLAMHDCDVMICIGARFDDRITGRLNAFSPNSKKIHIDIDPSSINKNVR
VDVPIIGDVAAVLEDMVRLWRAAAKTVDRTRLEDWWKSITKWRARNSLAYTPSADVIMPQYAIQRLYELTKDRDTYITTE
VGQHQMWAAQFFGFEEPNRWMTSGGLGTMGYGFPAAVGVQVAHPDSLVIDIAGDASIQMCIQEMSCAVQYGLPVKIFILN
NQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGVGIRCEKPGELDEAIKQMIDTPAPVIFDCRVANLANCFPMI
PSGKAHNEMLLPDEATDEAVANAIDAKGRQLV
>Mature_591_residues
SGTENQMTGAEIVLQALKDNGVEHIFGYPGGAVLPIYDEIFQQEDIQHILVRHEQGAGHMAEGYARSTGKVGVMLVTSGP
GATNAVTPLQDALMDSIPLVCISGQVPTSLIGSDAFQECDTVGITRPCTKHNWLVRDVNELARVIHEAFRVAQSGRPGPV
VVDIPKDIQFATGAYTPPSAVPTQKSYQPKTQGDLKKIEEAVALMKSARQPVIYSGGGVINSGPQAAHFLRELVELTDFP
ITSTLMGLGAYPASGKNWLGMLGMHGTYEANLAMHDCDVMICIGARFDDRITGRLNAFSPNSKKIHIDIDPSSINKNVRV
DVPIIGDVAAVLEDMVRLWRAAAKTVDRTRLEDWWKSITKWRARNSLAYTPSADVIMPQYAIQRLYELTKDRDTYITTEV
GQHQMWAAQFFGFEEPNRWMTSGGLGTMGYGFPAAVGVQVAHPDSLVIDIAGDASIQMCIQEMSCAVQYGLPVKIFILNN
QYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGVGIRCEKPGELDEAIKQMIDTPAPVIFDCRVANLANCFPMIP
SGKAHNEMLLPDEATDEAVANAIDAKGRQLV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=573, Percent_Identity=25.6544502617801, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI21361361, Length=569, Percent_Identity=25.3075571177504, Blast_Score=142, Evalue=7e-34,
Organism=Escherichia coli, GI87081685, Length=570, Percent_Identity=50.8771929824561, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1790104, Length=569, Percent_Identity=41.4762741652021, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI1786717, Length=570, Percent_Identity=34.7368421052632, Blast_Score=311, Evalue=7e-86,
Organism=Escherichia coli, GI1787096, Length=560, Percent_Identity=29.2857142857143, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI1788716, Length=564, Percent_Identity=27.1276595744681, Blast_Score=158, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17531299, Length=571, Percent_Identity=26.6199649737303, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=571, Percent_Identity=26.6199649737303, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=523, Percent_Identity=27.5334608030593, Blast_Score=135, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=585, Percent_Identity=45.4700854700855, Blast_Score=508, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6320816, Length=497, Percent_Identity=25.1509054325956, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922626, Length=567, Percent_Identity=25.9259259259259, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64734; Mature: 64603

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTENQMTGAEIVLQALKDNGVEHIFGYPGGAVLPIYDEIFQQEDIQHILVRHEQGAGH
CCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCC
MAEGYARSTGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCISGQVPTSLIGSDAFQEC
HHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHCCHHHHHH
DTVGITRPCTKHNWLVRDVNELARVIHEAFRVAQSGRPGPVVVDIPKDIQFATGAYTPPS
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCCCCCC
AVPTQKSYQPKTQGDLKKIEEAVALMKSARQPVIYSGGGVINSGPQAAHFLRELVELTDF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCC
PITSTLMGLGAYPASGKNWLGMLGMHGTYEANLAMHDCDVMICIGARFDDRITGRLNAFS
CHHHHHHHCCCCCCCCCCCEEECCCCCCEECCEEEECCCEEEEECCCCCCCCCCEEECCC
PNSKKIHIDIDPSSINKNVRVDVPIIGDVAAVLEDMVRLWRAAAKTVDRTRLEDWWKSIT
CCCCEEEEEECHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KWRARNSLAYTPSADVIMPQYAIQRLYELTKDRDTYITTEVGQHQMWAAQFFGFEEPNRW
HHHHCCCEEECCCCCEECHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCE
MTSGGLGTMGYGFPAAVGVQVAHPDSLVIDIAGDASIQMCIQEMSCAVQYGLPVKIFILN
EECCCCCCCCCCCCHHHEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEC
NQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGVGIRCEKPGELDEAIKQMIDT
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCC
PAPVIFDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRQLV
CCCEEEECCHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SGTENQMTGAEIVLQALKDNGVEHIFGYPGGAVLPIYDEIFQQEDIQHILVRHEQGAGH
CCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCC
MAEGYARSTGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCISGQVPTSLIGSDAFQEC
HHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHCCHHHHHH
DTVGITRPCTKHNWLVRDVNELARVIHEAFRVAQSGRPGPVVVDIPKDIQFATGAYTPPS
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCCCCCC
AVPTQKSYQPKTQGDLKKIEEAVALMKSARQPVIYSGGGVINSGPQAAHFLRELVELTDF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCC
PITSTLMGLGAYPASGKNWLGMLGMHGTYEANLAMHDCDVMICIGARFDDRITGRLNAFS
CHHHHHHHCCCCCCCCCCCEEECCCCCCEECCEEEECCCEEEEECCCCCCCCCCEEECCC
PNSKKIHIDIDPSSINKNVRVDVPIIGDVAAVLEDMVRLWRAAAKTVDRTRLEDWWKSIT
CCCCEEEEEECHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KWRARNSLAYTPSADVIMPQYAIQRLYELTKDRDTYITTEVGQHQMWAAQFFGFEEPNRW
HHHHCCCEEECCCCCEECHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCE
MTSGGLGTMGYGFPAAVGVQVAHPDSLVIDIAGDASIQMCIQEMSCAVQYGLPVKIFILN
EECCCCCCCCCCCCHHHEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEC
NQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGVGIRCEKPGELDEAIKQMIDT
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCC
PAPVIFDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRQLV
CCCEEEECCHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]