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The map label for this gene is pdxJ [H]

Identifier: 150397184

GI number: 150397184

Start: 2033982

End: 2034734

Strand: Reverse

Name: pdxJ [H]

Synonym: Smed_1982

Alternate gene names: 150397184

Gene position: 2034734-2033982 (Counterclockwise)

Preceding gene: 150397189

Following gene: 150397183

Centisome position: 53.8

GC content: 62.28

Gene sequence:

>753_bases
ATGCCTGCAAAGCTCTCCGTAAATCTCAACGCAATTGCAATGCTGCGTAACCGGCGCGATCTCCCCTGGCCCTCGGTGAC
CGGGCTGGGCCGCATCGCCCTGCAGGCGGGAGCGAGCGGTCTGACCGTTCATCCGCGGCCGGACCAGAGGCATATTCGCT
TTTCCGACCTCCAACCGATCCGCAACCTGATAGACGACGAGTTTCCGGCCGCTGAATTCAACATGGAGGGCTTTCCGAAC
GAGGCGTTTCTCGAACTCGTCGAGCGACATGAACCGGAGCAGGTGACATTGGTGCCGGACGATCCGGCGCAAGCGACCTC
CGACCATGGCTGGGACTTTCACAAGAGCCATAACCTGCTCGGCAACGTTGTCGGGAGTCTTAAGAAGAAGGGCTACCGCG
TCTCGCTCTTTGCTGATGGCACCCCCGACCTGCAAGCGCTGAAAATCGCGAAGGAGACGGGGGCCGACCGGATTGAGCTT
TATACAGGACCCTACGGCGGCTGTTACGATGATCCGGAGAAGGCGGAGCGGGTCGCGACCGAGTTGGGCCGCACCGCGGA
GATCGCGATCGGCCTCGGGCTTGCCGTCAATGCGGGCCACGATCTGACCGTCGCAAACCTGCCGTTGCTCGTCAAATACA
TCCCCGAACTCGCCGAGGTCTCGATCGGCCATGGGCTGACGGCAGATGCGCTCGAATATGGCATGGCCGAAACCGTGCGA
CGCTTTCGCCGCGCCTGCGGCGAGGCGGCTTAG

Upstream 100 bases:

>100_bases
TGCAAAGACCGTTACCTGTTCCCGAAATCTGCGCTAGAACAGCAGCGCAATCATCATTCGGCCCCTTTTGCGATCGGGGC
ATTTCGTCTGAAAGTTCGAC

Downstream 100 bases:

>100_bases
AAACATCCGAAGGTTTGAGGAGAGATACTCCTCTTTCATCGCTAGCCACTTGACGAATGGCTGCCTGCGTGTTTTTTGGA
ATCGAATTGCGCAGACGGCG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MPAKLSVNLNAIAMLRNRRDLPWPSVTGLGRIALQAGASGLTVHPRPDQRHIRFSDLQPIRNLIDDEFPAAEFNMEGFPN
EAFLELVERHEPEQVTLVPDDPAQATSDHGWDFHKSHNLLGNVVGSLKKKGYRVSLFADGTPDLQALKIAKETGADRIEL
YTGPYGGCYDDPEKAERVATELGRTAEIAIGLGLAVNAGHDLTVANLPLLVKYIPELAEVSIGHGLTADALEYGMAETVR
RFRRACGEAA

Sequences:

>Translated_250_residues
MPAKLSVNLNAIAMLRNRRDLPWPSVTGLGRIALQAGASGLTVHPRPDQRHIRFSDLQPIRNLIDDEFPAAEFNMEGFPN
EAFLELVERHEPEQVTLVPDDPAQATSDHGWDFHKSHNLLGNVVGSLKKKGYRVSLFADGTPDLQALKIAKETGADRIEL
YTGPYGGCYDDPEKAERVATELGRTAEIAIGLGLAVNAGHDLTVANLPLLVKYIPELAEVSIGHGLTADALEYGMAETVR
RFRRACGEAA
>Mature_249_residues
PAKLSVNLNAIAMLRNRRDLPWPSVTGLGRIALQAGASGLTVHPRPDQRHIRFSDLQPIRNLIDDEFPAAEFNMEGFPNE
AFLELVERHEPEQVTLVPDDPAQATSDHGWDFHKSHNLLGNVVGSLKKKGYRVSLFADGTPDLQALKIAKETGADRIELY
TGPYGGCYDDPEKAERVATELGRTAEIAIGLGLAVNAGHDLTVANLPLLVKYIPELAEVSIGHGLTADALEYGMAETVRR
FRRACGEAA

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate [H]

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788917, Length=245, Percent_Identity=35.5102040816327, Blast_Score=135, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004569 [H]

Pfam domain/function: PF03740 PdxJ [H]

EC number: =2.6.99.2 [H]

Molecular weight: Translated: 27269; Mature: 27138

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKLSVNLNAIAMLRNRRDLPWPSVTGLGRIALQAGASGLTVHPRPDQRHIRFSDLQPI
CCCEEECCHHHHEEECCCCCCCCCCCCCCHHHHHHCCCCCCEECCCCCCCCEEHHHHHHH
RNLIDDEFPAAEFNMEGFPNEAFLELVERHEPEQVTLVPDDPAQATSDHGWDFHKSHNLL
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHH
GNVVGSLKKKGYRVSLFADGTPDLQALKIAKETGADRIELYTGPYGGCYDDPEKAERVAT
HHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHH
ELGRTAEIAIGLGLAVNAGHDLTVANLPLLVKYIPELAEVSIGHGLTADALEYGMAETVR
HHCCCEEEEEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RFRRACGEAA
HHHHHHCCCC
>Mature Secondary Structure 
PAKLSVNLNAIAMLRNRRDLPWPSVTGLGRIALQAGASGLTVHPRPDQRHIRFSDLQPI
CCEEECCHHHHEEECCCCCCCCCCCCCCHHHHHHCCCCCCEECCCCCCCCEEHHHHHHH
RNLIDDEFPAAEFNMEGFPNEAFLELVERHEPEQVTLVPDDPAQATSDHGWDFHKSHNLL
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHH
GNVVGSLKKKGYRVSLFADGTPDLQALKIAKETGADRIELYTGPYGGCYDDPEKAERVAT
HHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHH
ELGRTAEIAIGLGLAVNAGHDLTVANLPLLVKYIPELAEVSIGHGLTADALEYGMAETVR
HHCCCEEEEEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RFRRACGEAA
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]