Click here to switch to the map view.

The map label for this gene is mtbC [H]

Identifier: 150396979

GI number: 150396979

Start: 1861761

End: 1862459

Strand: Direct

Name: mtbC [H]

Synonym: Smed_1776

Alternate gene names: 150396979

Gene position: 1861761-1862459 (Clockwise)

Preceding gene: 150396974

Following gene: 150396980

Centisome position: 49.23

GC content: 57.8

Gene sequence:

>699_bases
ATGGCAGACGATGAAATCATTCTCGAGGACCTTTCCGATGAGGAACTCGTGCAGCAGATGCATGACGACCTTTATGACGG
TCTCAAAGAGGAAATCGAGGAAGCGACGCGAATCCTCCTCAAGCGTGGCTGGACGCCATACGACGTCCTGACCCAAGCCC
TGGTCGAAGGCATGCGCATCGTCGGGATAGATTTCCGCGACGGCATTCTCTTTGTTCCGGAAGTGCTGCTTTCCGCCAAT
GCGATGAAGGCCGGTATGTTCATCCTGCGCCCGCTTCTCGTCGAGACTGGAGCGCCGAAGCTCGGAAAGATGGTTATCGG
TACGGTCAAGGGCGACATCCACGACATCGGCAAGAACCTCGTCGGCATGATGATGGAAGGCGCCGGCTTCGATGTCGTCG
ACCTCGGTATCAACAACCCGGTTGAAAACTACCTCGAAGCGCTCGAGCGTGAGAAGCCCGACATCCTCGGCATGTCGGCA
CTGCTCACGACCACCATGCCCTATATGAAGGTCGTTATCGACACGATGAAAGAGAAGGGGTTGCGCGACGAATACGTCGT
CCTCGTGGGCGGTGCACCGCTCAATGAGGAGTTCGGCAAGGCCGTCGGCGCTGACGCCTATTGCCGCGATGCCGCAGTCG
CAGTCGAGACCGCCAAGGACTACATGAAGCGTAAGCACAACCAGCTTGCAGCCGGCTGA

Upstream 100 bases:

>100_bases
TTAGGTGTAGCCCGCTAAGGATTGGGGAACGCGTCCGGATTGCGACCTCTCTCGTGCCACCCGTGCGAAATGACTGTGCC
AGGAACCGAGGAACCCAACT

Downstream 100 bases:

>100_bases
GACGATTCGACATGAAAAAGCCGGCTGCTCGTGTGAAACGAGCAGCCGGCTTTTCTGTTTTTAGCGACGAGGAGAGCGTG
AACGCGGCGGCTCTATCGCG

Product: methionine synthase

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MADDEIILEDLSDEELVQQMHDDLYDGLKEEIEEATRILLKRGWTPYDVLTQALVEGMRIVGIDFRDGILFVPEVLLSAN
AMKAGMFILRPLLVETGAPKLGKMVIGTVKGDIHDIGKNLVGMMMEGAGFDVVDLGINNPVENYLEALEREKPDILGMSA
LLTTTMPYMKVVIDTMKEKGLRDEYVVLVGGAPLNEEFGKAVGADAYCRDAAVAVETAKDYMKRKHNQLAAG

Sequences:

>Translated_232_residues
MADDEIILEDLSDEELVQQMHDDLYDGLKEEIEEATRILLKRGWTPYDVLTQALVEGMRIVGIDFRDGILFVPEVLLSAN
AMKAGMFILRPLLVETGAPKLGKMVIGTVKGDIHDIGKNLVGMMMEGAGFDVVDLGINNPVENYLEALEREKPDILGMSA
LLTTTMPYMKVVIDTMKEKGLRDEYVVLVGGAPLNEEFGKAVGADAYCRDAAVAVETAKDYMKRKHNQLAAG
>Mature_231_residues
ADDEIILEDLSDEELVQQMHDDLYDGLKEEIEEATRILLKRGWTPYDVLTQALVEGMRIVGIDFRDGILFVPEVLLSANA
MKAGMFILRPLLVETGAPKLGKMVIGTVKGDIHDIGKNLVGMMMEGAGFDVVDLGINNPVENYLEALEREKPDILGMSAL
LTTTMPYMKVVIDTMKEKGLRDEYVVLVGGAPLNEEFGKAVGADAYCRDAAVAVETAKDYMKRKHNQLAAG

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=176, Percent_Identity=36.9318181818182, Blast_Score=101, Evalue=6e-22,
Organism=Escherichia coli, GI1790450, Length=181, Percent_Identity=35.9116022099448, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17535405, Length=160, Percent_Identity=38.75, Blast_Score=103, Evalue=8e-23,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 25534; Mature: 25403

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDEIILEDLSDEELVQQMHDDLYDGLKEEIEEATRILLKRGWTPYDVLTQALVEGMRI
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEE
VGIDFRDGILFVPEVLLSANAMKAGMFILRPLLVETGAPKLGKMVIGTVKGDIHDIGKNL
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VGMMMEGAGFDVVDLGINNPVENYLEALEREKPDILGMSALLTTTMPYMKVVIDTMKEKG
HHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
LRDEYVVLVGGAPLNEEFGKAVGADAYCRDAAVAVETAKDYMKRKHNQLAAG
CCCCEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADDEIILEDLSDEELVQQMHDDLYDGLKEEIEEATRILLKRGWTPYDVLTQALVEGMRI
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEE
VGIDFRDGILFVPEVLLSANAMKAGMFILRPLLVETGAPKLGKMVIGTVKGDIHDIGKNL
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VGMMMEGAGFDVVDLGINNPVENYLEALEREKPDILGMSALLTTTMPYMKVVIDTMKEKG
HHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
LRDEYVVLVGGAPLNEEFGKAVGADAYCRDAAVAVETAKDYMKRKHNQLAAG
CCCCEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} 0.035 {S-methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10762254; 10852929 [H]