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The map label for this gene is potH [H]

Identifier: 150396920

GI number: 150396920

Start: 1796739

End: 1797920

Strand: Direct

Name: potH [H]

Synonym: Smed_1717

Alternate gene names: 150396920

Gene position: 1796739-1797920 (Clockwise)

Preceding gene: 150396919

Following gene: 150396921

Centisome position: 47.51

GC content: 59.56

Gene sequence:

>1182_bases
ATGAAGCAACGATCCTGGCTCCTGCGGCACGCCAAATCCTTCGTCCTGATTGCGCCACTGGCGCTATTTCTTGCCTTCTT
CTTCGTTGCCCCGCTGGTAACCATGATGAAGACGGCCGTCTCCGATCCCGTCGCTTCTCGGGCGCTTCCGAATATCGCCG
CTGCCCTCGACGGCTGGGATCGTACAAGCGCCCCGCCGGCTGATGCGCAGAGAGCGTTGGTCGCGGATATCCGGGGAATA
CAGGAGGATGAACTGTTCGGTGACCTGGTTCGCAGGCTGAACAGCGCGAAGTCCGGTTTCCGTACTCTGATGGGCAAGAC
CAGAAAGGCCGTTGCCGAGAACCCGAACCTCGATGACCTGATTTCCGTCGACAAGCGATGGAGCGACCCGGCATTTTGGA
TTGCGGTGCAGGATGCGGCGAGCTCGCCCGTCACCGACAGGAACCTTCTTGCAGCTGTCGATCTGACGCGCGATTTGAAT
GGCAATATCATCGAAGCGCCTGCCGGTACCTCGGCGAACCGCGAAACGCTGATTCGCACCATTGTCATGGCCGGCATGGT
AACCATATGCACCGTTCTGATCGGGCTGCCTTTCGCGATGGTTGCGGCCTCGACCACAGGATGGAAACGTCAACTCTTGC
TCGGAGCGGTGCTGCTCCCGCTCTGGACGTCGCTCCTCGTCAGGACGGCCGCCTGGTACATCATTCTGCAAGACAACGGC
TTGATCAATTTAACACTCCAGGCGATCGGCCTGACCGAAGGGCCATTGCCTCTGATCTTCAATCGGCTCGGCGTCGTCAT
CGCGATGACCCATGTCCTGCTTCCATTCATGGTGCTGCCGATCTTCAGTGTCTTGATCGGCATCCCCAAGAACCTCATGC
CGGCTGCGGCCTCGTTGGGCGCTTCACCTGTCCGGTCATTCCTTTCGGTGCTTCTGCCCCTCAGCCTGCGAGGCGTCGTG
TCCGGATCGCTGCTCGTATTCATGAGCGCTCTTGGCTACTACATCACGCCCGCACTGATCGGCGGTGCAAAGGACCAGAT
GATCAGCGCCGTGATCGCTTATTATGCGACGGGGGCGGCCAACTGGGGCATGGCCGGCGCGCTCGGAATCGTTCTGCTGA
CCGCCACGATCATTCTGTATGTCGTGTATTTGCGGCTCTCATCCGAGGAGGCAAAGGCATGA

Upstream 100 bases:

>100_bases
GCGGACCACTCGTCGTAAAATCGACCAGGAAAGCAAGCGCGCCTCGTATCGGCGATGAGGCGGTTGCTTCATTCTCCCCC
GAACAATGCTGGATCGTCGC

Downstream 100 bases:

>100_bases
AAGCGCTTCCTCTATCCAAGGCAGTATTCGGCTCTGCCGTCGTCGTCTTTCTGATGGCACCACTGTTTGCGATCCTGCCA
TTGGCGTTCACGGATAGCGT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKQRSWLLRHAKSFVLIAPLALFLAFFFVAPLVTMMKTAVSDPVASRALPNIAAALDGWDRTSAPPADAQRALVADIRGI
QEDELFGDLVRRLNSAKSGFRTLMGKTRKAVAENPNLDDLISVDKRWSDPAFWIAVQDAASSPVTDRNLLAAVDLTRDLN
GNIIEAPAGTSANRETLIRTIVMAGMVTICTVLIGLPFAMVAASTTGWKRQLLLGAVLLPLWTSLLVRTAAWYIILQDNG
LINLTLQAIGLTEGPLPLIFNRLGVVIAMTHVLLPFMVLPIFSVLIGIPKNLMPAAASLGASPVRSFLSVLLPLSLRGVV
SGSLLVFMSALGYYITPALIGGAKDQMISAVIAYYATGAANWGMAGALGIVLLTATIILYVVYLRLSSEEAKA

Sequences:

>Translated_393_residues
MKQRSWLLRHAKSFVLIAPLALFLAFFFVAPLVTMMKTAVSDPVASRALPNIAAALDGWDRTSAPPADAQRALVADIRGI
QEDELFGDLVRRLNSAKSGFRTLMGKTRKAVAENPNLDDLISVDKRWSDPAFWIAVQDAASSPVTDRNLLAAVDLTRDLN
GNIIEAPAGTSANRETLIRTIVMAGMVTICTVLIGLPFAMVAASTTGWKRQLLLGAVLLPLWTSLLVRTAAWYIILQDNG
LINLTLQAIGLTEGPLPLIFNRLGVVIAMTHVLLPFMVLPIFSVLIGIPKNLMPAAASLGASPVRSFLSVLLPLSLRGVV
SGSLLVFMSALGYYITPALIGGAKDQMISAVIAYYATGAANWGMAGALGIVLLTATIILYVVYLRLSSEEAKA
>Mature_393_residues
MKQRSWLLRHAKSFVLIAPLALFLAFFFVAPLVTMMKTAVSDPVASRALPNIAAALDGWDRTSAPPADAQRALVADIRGI
QEDELFGDLVRRLNSAKSGFRTLMGKTRKAVAENPNLDDLISVDKRWSDPAFWIAVQDAASSPVTDRNLLAAVDLTRDLN
GNIIEAPAGTSANRETLIRTIVMAGMVTICTVLIGLPFAMVAASTTGWKRQLLLGAVLLPLWTSLLVRTAAWYIILQDNG
LINLTLQAIGLTEGPLPLIFNRLGVVIAMTHVLLPFMVLPIFSVLIGIPKNLMPAAASLGASPVRSFLSVLLPLSLRGVV
SGSLLVFMSALGYYITPALIGGAKDQMISAVIAYYATGAANWGMAGALGIVLLTATIILYVVYLRLSSEEAKA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=240, Percent_Identity=35.4166666666667, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1787369, Length=213, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1787713, Length=236, Percent_Identity=32.6271186440678, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 42062; Mature: 42062

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQRSWLLRHAKSFVLIAPLALFLAFFFVAPLVTMMKTAVSDPVASRALPNIAAALDGWD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
RTSAPPADAQRALVADIRGIQEDELFGDLVRRLNSAKSGFRTLMGKTRKAVAENPNLDDL
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ISVDKRWSDPAFWIAVQDAASSPVTDRNLLAAVDLTRDLNGNIIEAPAGTSANRETLIRT
HHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHH
IVMAGMVTICTVLIGLPFAMVAASTTGWKRQLLLGAVLLPLWTSLLVRTAAWYIILQDNG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
LINLTLQAIGLTEGPLPLIFNRLGVVIAMTHVLLPFMVLPIFSVLIGIPKNLMPAAASLG
EEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
ASPVRSFLSVLLPLSLRGVVSGSLLVFMSALGYYITPALIGGAKDQMISAVIAYYATGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
NWGMAGALGIVLLTATIILYVVYLRLSSEEAKA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure
MKQRSWLLRHAKSFVLIAPLALFLAFFFVAPLVTMMKTAVSDPVASRALPNIAAALDGWD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
RTSAPPADAQRALVADIRGIQEDELFGDLVRRLNSAKSGFRTLMGKTRKAVAENPNLDDL
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ISVDKRWSDPAFWIAVQDAASSPVTDRNLLAAVDLTRDLNGNIIEAPAGTSANRETLIRT
HHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHH
IVMAGMVTICTVLIGLPFAMVAASTTGWKRQLLLGAVLLPLWTSLLVRTAAWYIILQDNG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
LINLTLQAIGLTEGPLPLIFNRLGVVIAMTHVLLPFMVLPIFSVLIGIPKNLMPAAASLG
EEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
ASPVRSFLSVLLPLSLRGVVSGSLLVFMSALGYYITPALIGGAKDQMISAVIAYYATGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
NWGMAGALGIVLLTATIILYVVYLRLSSEEAKA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]