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The map label for this gene is potD [C]

Identifier: 150396918

GI number: 150396918

Start: 1794538

End: 1795578

Strand: Direct

Name: potD [C]

Synonym: Smed_1715

Alternate gene names: 150396918

Gene position: 1794538-1795578 (Clockwise)

Preceding gene: 150396917

Following gene: 150396919

Centisome position: 47.45

GC content: 57.73

Gene sequence:

>1041_bases
TTGAAAATTCTCAAAGCCACTATCATTGTCTCCAGCGTCCTGATGACAGGCACCTTCGGCGCGCAAGCGCGCGACCTCAC
CGTCGTGTCCTGGGGCGGAAATTTCCAGGATGCGCAACGAAAAATATTCTTCGAAACTTTTGCCAAGGAAACCGGGAAAC
CTGTCCTCGATGAAAGCTGGGAGGGTGGATACGGTGTGTTGCAGGCCAAGGTAAAGGCCGGTATCCCGAACTGGGACGTA
GTCGAAGTCGAAGCCGAAGAATTGGCGCTGGGTTGTGCCGACGGCATCTTCGAGAAGATCGACTGGCAGAAAATGGGCGG
TAAGGAAGCATATCTTCCCGCCGCCGTCAGCGACTGCGGGGTCGGCAATATAGTTTGGTCGACGGGCCTGTCTTATGACG
GTGACAAGCTTGCAGAGGGTCCGAAGACCTGGGCCGATTTCTGGGATACCAAGAAATTCCCCGGCAAGCGCGGCCTGCGC
AAGGGACCGAAATACGCGCTCGAGTTCGCTCTGATGGCCGATGGCGTCGCCCCCGACCAGGTGTACGATGTGCTCAGTGG
CGAGGGCGGCGTCGATCGCGCCTTCAAGAAGCTCGATGAATTGAAGGCCGATATTATCTGGTGGGACGCCGGGGCGCAGC
CGCTTCAACTGTTGTCTTCCGGCCAGGTCGTGATGACGTCGGCATATAACGGCCGGATCAGCGGTATCAACCGGAGCGAG
GGCAAGAAGTTCGGCTTCGTTTTCCCGGGCAGCGTCTATGCGATCGACAGCTGGGTCATCCTCAAGGACAGCCCGAACAA
GGACGCGGGCATGGACTTCATCGCCTATGCCAGCAAGGCCGAAAACCAGGCGAAGCTTCCTGAGTTCATCGCATATGGCC
TACCGAACCTCGGGGCCACCGAACTTGTCCCGCAACAGTTTCAGGAAGAGCTGCCTACAACGGAAAAGAACCTGAATGGT
GCGGTCGCCCTCGATGTGGATTTCTGGACCGACAACTCCGAAGAACTGACCCAGCGGTTCAACGCCTGGCTCGCTCAGTA
A

Upstream 100 bases:

>100_bases
TCCGTGGTGAGAATAGTAACCAGGGCGGTTATCCCCACTTCCCAAACCTCCTAAACCTGCTTTCTATCCCTCCAGTCGAA
ATTAATGGGGAGCGCGTCCG

Downstream 100 bases:

>100_bases
GCGGCCTTACCTCGCGCCGCTCTGCCGCCCCTGCTGTAGGGGCGGTATAACCCTTTTGTCAATCAGGGGGCGGCCCTTCT
CAAGTTTGCGTGGGTGATGA

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: None

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKILKATIIVSSVLMTGTFGAQARDLTVVSWGGNFQDAQRKIFFETFAKETGKPVLDESWEGGYGVLQAKVKAGIPNWDV
VEVEAEELALGCADGIFEKIDWQKMGGKEAYLPAAVSDCGVGNIVWSTGLSYDGDKLAEGPKTWADFWDTKKFPGKRGLR
KGPKYALEFALMADGVAPDQVYDVLSGEGGVDRAFKKLDELKADIIWWDAGAQPLQLLSSGQVVMTSAYNGRISGINRSE
GKKFGFVFPGSVYAIDSWVILKDSPNKDAGMDFIAYASKAENQAKLPEFIAYGLPNLGATELVPQQFQEELPTTEKNLNG
AVALDVDFWTDNSEELTQRFNAWLAQ

Sequences:

>Translated_346_residues
MKILKATIIVSSVLMTGTFGAQARDLTVVSWGGNFQDAQRKIFFETFAKETGKPVLDESWEGGYGVLQAKVKAGIPNWDV
VEVEAEELALGCADGIFEKIDWQKMGGKEAYLPAAVSDCGVGNIVWSTGLSYDGDKLAEGPKTWADFWDTKKFPGKRGLR
KGPKYALEFALMADGVAPDQVYDVLSGEGGVDRAFKKLDELKADIIWWDAGAQPLQLLSSGQVVMTSAYNGRISGINRSE
GKKFGFVFPGSVYAIDSWVILKDSPNKDAGMDFIAYASKAENQAKLPEFIAYGLPNLGATELVPQQFQEELPTTEKNLNG
AVALDVDFWTDNSEELTQRFNAWLAQ
>Mature_346_residues
MKILKATIIVSSVLMTGTFGAQARDLTVVSWGGNFQDAQRKIFFETFAKETGKPVLDESWEGGYGVLQAKVKAGIPNWDV
VEVEAEELALGCADGIFEKIDWQKMGGKEAYLPAAVSDCGVGNIVWSTGLSYDGDKLAEGPKTWADFWDTKKFPGKRGLR
KGPKYALEFALMADGVAPDQVYDVLSGEGGVDRAFKKLDELKADIIWWDAGAQPLQLLSSGQVVMTSAYNGRISGINRSE
GKKFGFVFPGSVYAIDSWVILKDSPNKDAGMDFIAYASKAENQAKLPEFIAYGLPNLGATELVPQQFQEELPTTEKNLNG
AVALDVDFWTDNSEELTQRFNAWLAQ

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37839; Mature: 37839

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILKATIIVSSVLMTGTFGAQARDLTVVSWGGNFQDAQRKIFFETFAKETGKPVLDESW
CCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
EGGYGVLQAKVKAGIPNWDVVEVEAEELALGCADGIFEKIDWQKMGGKEAYLPAAVSDCG
CCCCEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
VGNIVWSTGLSYDGDKLAEGPKTWADFWDTKKFPGKRGLRKGPKYALEFALMADGVAPDQ
CCCEEEECCCCCCCHHHCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHEEHHHHCCCCHHH
VYDVLSGEGGVDRAFKKLDELKADIIWWDAGAQPLQLLSSGQVVMTSAYNGRISGINRSE
HHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCEEEEECCCCEECCCCCCC
GKKFGFVFPGSVYAIDSWVILKDSPNKDAGMDFIAYASKAENQAKLPEFIAYGLPNLGAT
CCEEEEECCCCEEEEEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHCCCCCCHH
ELVPQQFQEELPTTEKNLNGAVALDVDFWTDNSEELTQRFNAWLAQ
HHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILKATIIVSSVLMTGTFGAQARDLTVVSWGGNFQDAQRKIFFETFAKETGKPVLDESW
CCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
EGGYGVLQAKVKAGIPNWDVVEVEAEELALGCADGIFEKIDWQKMGGKEAYLPAAVSDCG
CCCCEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
VGNIVWSTGLSYDGDKLAEGPKTWADFWDTKKFPGKRGLRKGPKYALEFALMADGVAPDQ
CCCEEEECCCCCCCHHHCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHEEHHHHCCCCHHH
VYDVLSGEGGVDRAFKKLDELKADIIWWDAGAQPLQLLSSGQVVMTSAYNGRISGINRSE
HHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCEEEEECCCCEECCCCCCC
GKKFGFVFPGSVYAIDSWVILKDSPNKDAGMDFIAYASKAENQAKLPEFIAYGLPNLGAT
CCEEEEECCCCEEEEEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHCCCCCCHH
ELVPQQFQEELPTTEKNLNGAVALDVDFWTDNSEELTQRFNAWLAQ
HHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA