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The map label for this gene is dusA [H]

Identifier: 150396830

GI number: 150396830

Start: 1716358

End: 1717515

Strand: Reverse

Name: dusA [H]

Synonym: Smed_1621

Alternate gene names: 150396830

Gene position: 1717515-1716358 (Counterclockwise)

Preceding gene: 150396832

Following gene: 150396828

Centisome position: 45.41

GC content: 62.18

Gene sequence:

>1158_bases
GTGAAGATTAGAACGCAAGTCGCTGAAAGAGCGAAGAGAACCGCGTATTTCAATGGTCCCGTTTTTGCCGTGGCGCCTAT
GATCGATTGGACTGATCGTCATTATCGCTTCTTCGCGCGCGTGCTTTCGCGCCATGCCCTGCTTTATACGGAGATGATCG
TCGCGGATGCGGTCCTGCGCGGCGACCGGGCAAAGCTGCTCGGCCACGACGCATCGGAACATCCGGTCGCGCTGCAGCTC
GGCGGCAACGACCCGGTGAAGATGGCGGAGGCTGCAAGGATAGGCGAAGCCTACGGCTACGACGAAATCAACATGAATGT
AGGCTGCCCTTCAGACCGGGTGCAGTCCGGCACCTTCGGCGCCTGTCTGATGCAGGAGCCCGCATTGGTCGCCGACTGCA
TCGCGGCGATGAAGGCGGCGGTCGAGATTCCGGTTACGGTCAAATGCCGTATCGGCGTCGACGATCAGGATCCGGAAGTG
GCGCTTCGCGATCTCGTCAAGTGCGCCAAGGATGCGGGAACGGATGGCGTTTGGGTGCATGCCCGCAAGGCTTGGCTGCA
GGGGCTGAGCCCGAGAGAGAACCGCGAGATTCCTCCGCTTGATTACGGGCTCGTCCACCGGCTGAAGGCGGAAAATGAGA
GTCTTTTCATTGGCATCAATGGGGGGCTTCAAGGGTTGGATCAGGCGCTGTCGCATCTCGATCCGCGGTCGTTGCCTGCA
GGGGAAGCCATGAAGGCTGCGACCGAGATCAAGAGCGGCGCTCCTCTCGATGGCGTGATGCTCGGCCGGGCGGCCTATCA
CGACAGTGGCCTTCTGACGGCCGCCGATGGATACTTCCGGCACCCGCTGACGGGTGCCGCGGCCGTGCCGACGGATCCCG
ACGGGTTCTCCGATCGTGACCACAACCTGTCGCTGGAATTCTGGTCGGGTGTTCGCGACGTCATGGAAGACTATGCGGCT
GCGCATATCGAGAACGGCGGTCGGCTGATCCACGTCACCCGCCATATGGTCGGGCTCTTTCAGGGCTGGCCCGGCGCCCG
GCGTTATCGCCAGATTCTCTCGACCAGGGCCACTCGCAAGGACGCGGGTCCGGAGGTGATCCATACCGCCTTCGATGCAG
TATTCCAAGCATTCACGGCGAGGCAGGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
GTGCCCATCTGAGTGCTACACAGCCTTCTCTAAGTGCTACAATGTAGCACTAGCACGACGGAAATGAGCAAGAAAGCGTA
AAAATGAGAGCAAATGTGAC

Downstream 100 bases:

>100_bases
GGATTTCGGTTCGGGGCGGCCCTCCAATACAAAACGGCGCCTCTTGCGAAGCGCCGTTCTTTCAAGCTCTGTCGAAGGCT
TAGAGAGCCTGGAGGTTGAC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MKIRTQVAERAKRTAYFNGPVFAVAPMIDWTDRHYRFFARVLSRHALLYTEMIVADAVLRGDRAKLLGHDASEHPVALQL
GGNDPVKMAEAARIGEAYGYDEINMNVGCPSDRVQSGTFGACLMQEPALVADCIAAMKAAVEIPVTVKCRIGVDDQDPEV
ALRDLVKCAKDAGTDGVWVHARKAWLQGLSPRENREIPPLDYGLVHRLKAENESLFIGINGGLQGLDQALSHLDPRSLPA
GEAMKAATEIKSGAPLDGVMLGRAAYHDSGLLTAADGYFRHPLTGAAAVPTDPDGFSDRDHNLSLEFWSGVRDVMEDYAA
AHIENGGRLIHVTRHMVGLFQGWPGARRYRQILSTRATRKDAGPEVIHTAFDAVFQAFTARQAAE

Sequences:

>Translated_385_residues
MKIRTQVAERAKRTAYFNGPVFAVAPMIDWTDRHYRFFARVLSRHALLYTEMIVADAVLRGDRAKLLGHDASEHPVALQL
GGNDPVKMAEAARIGEAYGYDEINMNVGCPSDRVQSGTFGACLMQEPALVADCIAAMKAAVEIPVTVKCRIGVDDQDPEV
ALRDLVKCAKDAGTDGVWVHARKAWLQGLSPRENREIPPLDYGLVHRLKAENESLFIGINGGLQGLDQALSHLDPRSLPA
GEAMKAATEIKSGAPLDGVMLGRAAYHDSGLLTAADGYFRHPLTGAAAVPTDPDGFSDRDHNLSLEFWSGVRDVMEDYAA
AHIENGGRLIHVTRHMVGLFQGWPGARRYRQILSTRATRKDAGPEVIHTAFDAVFQAFTARQAAE
>Mature_385_residues
MKIRTQVAERAKRTAYFNGPVFAVAPMIDWTDRHYRFFARVLSRHALLYTEMIVADAVLRGDRAKLLGHDASEHPVALQL
GGNDPVKMAEAARIGEAYGYDEINMNVGCPSDRVQSGTFGACLMQEPALVADCIAAMKAAVEIPVTVKCRIGVDDQDPEV
ALRDLVKCAKDAGTDGVWVHARKAWLQGLSPRENREIPPLDYGLVHRLKAENESLFIGINGGLQGLDQALSHLDPRSLPA
GEAMKAATEIKSGAPLDGVMLGRAAYHDSGLLTAADGYFRHPLTGAAAVPTDPDGFSDRDHNLSLEFWSGVRDVMEDYAA
AHIENGGRLIHVTRHMVGLFQGWPGARRYRQILSTRATRKDAGPEVIHTAFDAVFQAFTARQAAE

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=214, Percent_Identity=31.3084112149533, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI145693211, Length=354, Percent_Identity=46.3276836158192, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1789660, Length=160, Percent_Identity=30.625, Blast_Score=76, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=167, Percent_Identity=28.7425149700599, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24580595, Length=174, Percent_Identity=27.0114942528736, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI19920448, Length=174, Percent_Identity=27.0114942528736, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 41990; Mature: 41990

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRTQVAERAKRTAYFNGPVFAVAPMIDWTDRHYRFFARVLSRHALLYTEMIVADAVLR
CCCHHHHHHHHHHCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDRAKLLGHDASEHPVALQLGGNDPVKMAEAARIGEAYGYDEINMNVGCPSDRVQSGTFG
CCHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCCC
ACLMQEPALVADCIAAMKAAVEIPVTVKCRIGVDDQDPEVALRDLVKCAKDAGTDGVWVH
HHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEE
ARKAWLQGLSPRENREIPPLDYGLVHRLKAENESLFIGINGGLQGLDQALSHLDPRSLPA
EHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCC
GEAMKAATEIKSGAPLDGVMLGRAAYHDSGLLTAADGYFRHPLTGAAAVPTDPDGFSDRD
CHHHHHHHHHCCCCCCCCEEECHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
HNLSLEFWSGVRDVMEDYAAAHIENGGRLIHVTRHMVGLFQGWPGARRYRQILSTRATRK
CCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
DAGPEVIHTAFDAVFQAFTARQAAE
CCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIRTQVAERAKRTAYFNGPVFAVAPMIDWTDRHYRFFARVLSRHALLYTEMIVADAVLR
CCCHHHHHHHHHHCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDRAKLLGHDASEHPVALQLGGNDPVKMAEAARIGEAYGYDEINMNVGCPSDRVQSGTFG
CCHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCCC
ACLMQEPALVADCIAAMKAAVEIPVTVKCRIGVDDQDPEVALRDLVKCAKDAGTDGVWVH
HHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEE
ARKAWLQGLSPRENREIPPLDYGLVHRLKAENESLFIGINGGLQGLDQALSHLDPRSLPA
EHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCC
GEAMKAATEIKSGAPLDGVMLGRAAYHDSGLLTAADGYFRHPLTGAAAVPTDPDGFSDRD
CHHHHHHHHHCCCCCCCCEEECHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
HNLSLEFWSGVRDVMEDYAAAHIENGGRLIHVTRHMVGLFQGWPGARRYRQILSTRATRK
CCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
DAGPEVIHTAFDAVFQAFTARQAAE
CCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]