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The map label for this gene is cspJ [H]

Identifier: 150396829

GI number: 150396829

Start: 1716066

End: 1716278

Strand: Direct

Name: cspJ [H]

Synonym: Smed_1620

Alternate gene names: 150396829

Gene position: 1716066-1716278 (Clockwise)

Preceding gene: 150396827

Following gene: 150396831

Centisome position: 45.38

GC content: 58.22

Gene sequence:

>213_bases
ATGGCCACCAAAGGCATCGTCAAGTTTTTCAACCAGGACAAGGGTTTTGGTTTCATCACGCCGGAAGGCGGTTCGAAGGA
CGTTTTCGTCCACATCACCGCTGTCCAGGCCGCAGGTCTCACCACGTTGAACGATGGCCAGGAGGTCAGCTTCGACACGG
AGCCGGATCGCATGGGCAAGGGCCCGAAGGCCGTCAACCTCCAGGCTCTCTAA

Upstream 100 bases:

>100_bases
TTCCTTCCGGGGGCGCGGACACACTACATAAAGCAACCGATGCGTGATAATGAAGCGCAGGGTTGCGGCAAAGACAGACT
GAAGGAAAAGGACTTCTTCC

Downstream 100 bases:

>100_bases
GCCTTCGACAGAGCTTGAAAGAACGGCGCTTCGCAAGAGGCGCCGTTTTGTATTGGAGGGCCGCCCCGAACCGAAATCCC
TACTCCGCCGCCTGCCTCGC

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 70; Mature: 69

Protein sequence:

>70_residues
MATKGIVKFFNQDKGFGFITPEGGSKDVFVHITAVQAAGLTTLNDGQEVSFDTEPDRMGKGPKAVNLQAL

Sequences:

>Translated_70_residues
MATKGIVKFFNQDKGFGFITPEGGSKDVFVHITAVQAAGLTTLNDGQEVSFDTEPDRMGKGPKAVNLQAL
>Mature_69_residues
ATKGIVKFFNQDKGFGFITPEGGSKDVFVHITAVQAAGLTTLNDGQEVSFDTEPDRMGKGPKAVNLQAL

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=66, Percent_Identity=59.0909090909091, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1786841, Length=67, Percent_Identity=59.7014925373134, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1788126, Length=67, Percent_Identity=56.7164179104478, Blast_Score=76, Evalue=5e-16,
Organism=Escherichia coli, GI2367114, Length=66, Percent_Identity=56.0606060606061, Blast_Score=76, Evalue=5e-16,
Organism=Escherichia coli, GI1787834, Length=66, Percent_Identity=56.0606060606061, Blast_Score=72, Evalue=8e-15,
Organism=Escherichia coli, GI1787839, Length=63, Percent_Identity=55.5555555555556, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI1787107, Length=60, Percent_Identity=51.6666666666667, Blast_Score=64, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7443; Mature: 7311

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKGIVKFFNQDKGFGFITPEGGSKDVFVHITAVQAAGLTTLNDGQEVSFDTEPDRMGK
CCCCCHHHCCCCCCCCEEECCCCCCCEEEEEEEEECCCCCEECCCCCEEECCCCHHHCCC
GPKAVNLQAL
CCCEEEEEEC
>Mature Secondary Structure 
ATKGIVKFFNQDKGFGFITPEGGSKDVFVHITAVQAAGLTTLNDGQEVSFDTEPDRMGK
CCCCHHHCCCCCCCCEEECCCCCCCEEEEEEEEECCCCCEECCCCCEEECCCCHHHCCC
GPKAVNLQAL
CCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]