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The map label for this gene is cobS

Identifier: 150396813

GI number: 150396813

Start: 1700237

End: 1701025

Strand: Direct

Name: cobS

Synonym: Smed_1604

Alternate gene names: 150396813

Gene position: 1700237-1701025 (Clockwise)

Preceding gene: 150396810

Following gene: 150396814

Centisome position: 44.96

GC content: 68.31

Gene sequence:

>789_bases
ATGACGGAAATTCGAGAGTTCTGGGACGACGTCGCACGGTCGGTCGCCTTCTTGAGCCGCATCCCCGTGCCGGATCGGCA
TTTCCGCGGCCATGACGGCGGACTCGGCCGCGCCGTGCGCGCCTTCCCGCTTGCGGGCATTCTGATCGCCCTCCCCGCCG
CCGTGACGGCGGTCCTTCTGGGCGCTATTCACGCGAGCTCGCTCTTCACCGCCTTCTTGATCGTCGCCGCCCAGGCGACG
GTCACGGGCGCCTTGCACGAGGACGGCCTTGCGGACACGGCCGATGGTTTCGGCGGCGGGCGCGACCGCGAAAGCGCGCT
GGAGATCATGAAAGACAGCCGGATCGGCACCTATGGCGCGGTCGCCCTTATTCTTTCCTTCGGAATTCGCGTCTCGGCTC
TTGCCGCCTTTCTGCCGCTGCTGACGCCGACAGGCGGCGGAGTTGCGCTGCTTGCTACCGCCGCGCTCAGCCGCGCCGCG
ATGGTCTGGCACTGGTCCCGCCTCCCGCCTGCCCGCCGCGACGGTGTGGCCGCGGCTGCGGGGGCTCCCGAGGCGCCAGC
GACGTCAGTGGCACTCGGCTCCGGGGTCATCCTCGCCCTTGTGCTGTTCTTTCTGTCCGGCATCCCGACCGTCGCCGTCC
TGCTTTCGTTCGGTGCCTTCGTGCTTGCCGTCCTCAGCTTTACCCGGATCGCATCACGCAAGCTCGGCGGCCATACCGGC
GACACCATCGGCGCCACCCAGCAACTGACGGAAGTCGCCGTTCTGGGCGCTCTTGCACTGGCGATCTGA

Upstream 100 bases:

>100_bases
CATAGAGCGTGAGGGCGCGTGCGGCAACGGCATTTGCGCCATTTGCCGTCGTCTCGCATGATCTCGGGAATGCCCGGCGA
TTCGGGATGCGGGAGACGAG

Downstream 100 bases:

>100_bases
AAGGCCGATATATTCAACGAAACAGAGCAGAGCATTATCCGGACCATGGAATCTCCCTGCATTCTAGTCTGCACGATCGA
CGACAGGACCGGTTACTGTT

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTEIREFWDDVARSVAFLSRIPVPDRHFRGHDGGLGRAVRAFPLAGILIALPAAVTAVLLGAIHASSLFTAFLIVAAQAT
VTGALHEDGLADTADGFGGGRDRESALEIMKDSRIGTYGAVALILSFGIRVSALAAFLPLLTPTGGGVALLATAALSRAA
MVWHWSRLPPARRDGVAAAAGAPEAPATSVALGSGVILALVLFFLSGIPTVAVLLSFGAFVLAVLSFTRIASRKLGGHTG
DTIGATQQLTEVAVLGALALAI

Sequences:

>Translated_262_residues
MTEIREFWDDVARSVAFLSRIPVPDRHFRGHDGGLGRAVRAFPLAGILIALPAAVTAVLLGAIHASSLFTAFLIVAAQAT
VTGALHEDGLADTADGFGGGRDRESALEIMKDSRIGTYGAVALILSFGIRVSALAAFLPLLTPTGGGVALLATAALSRAA
MVWHWSRLPPARRDGVAAAAGAPEAPATSVALGSGVILALVLFFLSGIPTVAVLLSFGAFVLAVLSFTRIASRKLGGHTG
DTIGATQQLTEVAVLGALALAI
>Mature_261_residues
TEIREFWDDVARSVAFLSRIPVPDRHFRGHDGGLGRAVRAFPLAGILIALPAAVTAVLLGAIHASSLFTAFLIVAAQATV
TGALHEDGLADTADGFGGGRDRESALEIMKDSRIGTYGAVALILSFGIRVSALAAFLPLLTPTGGGVALLATAALSRAAM
VWHWSRLPPARRDGVAAAAGAPEAPATSVALGSGVILALVLFFLSGIPTVAVLLSFGAFVLAVLSFTRIASRKLGGHTGD
TIGATQQLTEVAVLGALALAI

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=258, Percent_Identity=34.1085271317829, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_SINMW (A6U9W5)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001327280.1
- STRING:   A6U9W5
- GeneID:   5322462
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_1604
- eggNOG:   COG0368
- HOGENOM:   HBG729392
- OMA:   AHEIFIS
- ProtClustDB:   PRK00235
- BioCyc:   SMED366394:SMED_1604-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 26752; Mature: 26621

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x220326dc)-; HASH(0x2140c034)-; HASH(0x221b72c4)-; HASH(0x221b7258)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIREFWDDVARSVAFLSRIPVPDRHFRGHDGGLGRAVRAFPLAGILIALPAAVTAVLL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIHASSLFTAFLIVAAQATVTGALHEDGLADTADGFGGGRDRESALEIMKDSRIGTYGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHH
VALILSFGIRVSALAAFLPLLTPTGGGVALLATAALSRAAMVWHWSRLPPARRDGVAAAA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GAPEAPATSVALGSGVILALVLFFLSGIPTVAVLLSFGAFVLAVLSFTRIASRKLGGHTG
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DTIGATQQLTEVAVLGALALAI
CCCHHHHHHHHHHHHHHHHHHH
>Mature Secondary Structure 
TEIREFWDDVARSVAFLSRIPVPDRHFRGHDGGLGRAVRAFPLAGILIALPAAVTAVLL
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIHASSLFTAFLIVAAQATVTGALHEDGLADTADGFGGGRDRESALEIMKDSRIGTYGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHH
VALILSFGIRVSALAAFLPLLTPTGGGVALLATAALSRAAMVWHWSRLPPARRDGVAAAA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GAPEAPATSVALGSGVILALVLFFLSGIPTVAVLLSFGAFVLAVLSFTRIASRKLGGHTG
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DTIGATQQLTEVAVLGALALAI
CCCHHHHHHHHHHHHHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA