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The map label for this gene is garR [C]

Identifier: 150396804

GI number: 150396804

Start: 1692037

End: 1692906

Strand: Direct

Name: garR [C]

Synonym: Smed_1595

Alternate gene names: 150396804

Gene position: 1692037-1692906 (Clockwise)

Preceding gene: 150396803

Following gene: 150396805

Centisome position: 44.74

GC content: 61.15

Gene sequence:

>870_bases
ATGGCCAAAGTCGCATTCATCGGTCTCGGCGTAATGGGATACCCGATGGCGGGTCACCTCAAGGCGCGCGGCGGTCATGA
TGTCACGGTCTACAACCGGACCGCGGCCAAGGCGGAGCAATGGGCCGCGCAATTCGGCGGCCGGACCGCTGCAACGCCGG
CCGAAGCGGCCAAGGGCCAGGAGTTCGTCTTCGCCTGCGTCGGCAATGACGACGATCTTCGTTCCGTTACCACGGGTGAA
AACGGCGCCTTCGAAACGATCACCCCCGGCACGATCTTCATCGACAATACGACCGCATCGGCCGAGGTTGCGCGCGAGCT
CTCTGCGGCGGCGGCTGCGAAAGGCGCGCATTTCATCGACGCACCGGTCTCCGGAGGCCAGGCAGGCGCGGAGAACGGTG
TGCTCACCGTCATGTGCGGCGGCGACGTGGAAGCGTTCGAGAAGGCAAAATCCGTTATCGATGCCTATGCGCGGATGGTC
GGTTTGATGGGTCCGGCGGGTGCCGGTCAACTCACCAAGATGATCAATCAGATTTGTATCGCGGGCCTCGTTCAGGGGCT
CGCAGAGGGAATTCACTTCGGCAAGAAGGCGGGACTCGACATCGAAAAGGTGATCGAGGTGATCTCCAAGGGAGCGGCCG
GATCCTGGCAGATGGAAAATCGCCACAAGACCATGAACGAAGGCAAATATGACTTCGGCTTTGCGGTCGACTGGATGCGC
AAGGATCTCGACATCGTCCTTGCCGAGGCGCGGCGTAATGGCGCCAAGCTGCCGGTGACCGCCCTGGTTGATCAATTCTA
CGGTGATATCCAGGCGCTTGGTGGCAATCGCTGGGATACGTCTTCCCTGCTTGCCCGCCTCGAAAAATGA

Upstream 100 bases:

>100_bases
AGGCGAGCCGATTGCCGGACAATTGGTGATCGCCCGCTTCAAGCCCTGATACCAGGCGCGCCGCCGAAATTCCGCCAACG
CAAACTGGAGTCTCAAGGAA

Downstream 100 bases:

>100_bases
TGCCTGCCCCGGAGTCGACGGCGCAGGAAATCATCGAACACCTCCGCGCCCTCGGCTCGGACGAGAACATCGCCGCAATG
GCGCGCTTCGGTATTGCGAC

Product: 6-phosphogluconate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MAKVAFIGLGVMGYPMAGHLKARGGHDVTVYNRTAAKAEQWAAQFGGRTAATPAEAAKGQEFVFACVGNDDDLRSVTTGE
NGAFETITPGTIFIDNTTASAEVARELSAAAAAKGAHFIDAPVSGGQAGAENGVLTVMCGGDVEAFEKAKSVIDAYARMV
GLMGPAGAGQLTKMINQICIAGLVQGLAEGIHFGKKAGLDIEKVIEVISKGAAGSWQMENRHKTMNEGKYDFGFAVDWMR
KDLDIVLAEARRNGAKLPVTALVDQFYGDIQALGGNRWDTSSLLARLEK

Sequences:

>Translated_289_residues
MAKVAFIGLGVMGYPMAGHLKARGGHDVTVYNRTAAKAEQWAAQFGGRTAATPAEAAKGQEFVFACVGNDDDLRSVTTGE
NGAFETITPGTIFIDNTTASAEVARELSAAAAAKGAHFIDAPVSGGQAGAENGVLTVMCGGDVEAFEKAKSVIDAYARMV
GLMGPAGAGQLTKMINQICIAGLVQGLAEGIHFGKKAGLDIEKVIEVISKGAAGSWQMENRHKTMNEGKYDFGFAVDWMR
KDLDIVLAEARRNGAKLPVTALVDQFYGDIQALGGNRWDTSSLLARLEK
>Mature_288_residues
AKVAFIGLGVMGYPMAGHLKARGGHDVTVYNRTAAKAEQWAAQFGGRTAATPAEAAKGQEFVFACVGNDDDLRSVTTGEN
GAFETITPGTIFIDNTTASAEVARELSAAAAAKGAHFIDAPVSGGQAGAENGVLTVMCGGDVEAFEKAKSVIDAYARMVG
LMGPAGAGQLTKMINQICIAGLVQGLAEGIHFGKKAGLDIEKVIEVISKGAAGSWQMENRHKTMNEGKYDFGFAVDWMRK
DLDIVLAEARRNGAKLPVTALVDQFYGDIQALGGNRWDTSSLLARLEK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=281, Percent_Identity=27.0462633451957, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI23308751, Length=293, Percent_Identity=27.3037542662116, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI145693186, Length=288, Percent_Identity=35.7638888888889, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=31.4685314685315, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1790315, Length=295, Percent_Identity=32.5423728813559, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789092, Length=295, Percent_Identity=29.8305084745763, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=29.4545454545455, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24655230, Length=296, Percent_Identity=28.3783783783784, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922568, Length=296, Percent_Identity=28.3783783783784, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=22.6950354609929, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30345; Mature: 30214

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAFIGLGVMGYPMAGHLKARGGHDVTVYNRTAAKAEQWAAQFGGRTAATPAEAAKGQ
CCEEEEEECCHHCCCCCCCEECCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHHCCCC
EFVFACVGNDDDLRSVTTGENGAFETITPGTIFIDNTTASAEVARELSAAAAAKGAHFID
EEEEEEECCCCCCCEEECCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEE
APVSGGQAGAENGVLTVMCGGDVEAFEKAKSVIDAYARMVGLMGPAGAGQLTKMINQICI
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
AGLVQGLAEGIHFGKKAGLDIEKVIEVISKGAAGSWQMENRHKTMNEGKYDFGFAVDWMR
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEECCHHHHHCCCCCCCCHHHHHHH
KDLDIVLAEARRNGAKLPVTALVDQFYGDIQALGGNRWDTSSLLARLEK
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AKVAFIGLGVMGYPMAGHLKARGGHDVTVYNRTAAKAEQWAAQFGGRTAATPAEAAKGQ
CEEEEEECCHHCCCCCCCEECCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHHCCCC
EFVFACVGNDDDLRSVTTGENGAFETITPGTIFIDNTTASAEVARELSAAAAAKGAHFID
EEEEEEECCCCCCCEEECCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEE
APVSGGQAGAENGVLTVMCGGDVEAFEKAKSVIDAYARMVGLMGPAGAGQLTKMINQICI
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
AGLVQGLAEGIHFGKKAGLDIEKVIEVISKGAAGSWQMENRHKTMNEGKYDFGFAVDWMR
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEECCHHHHHCCCCCCCCHHHHHHH
KDLDIVLAEARRNGAKLPVTALVDQFYGDIQALGGNRWDTSSLLARLEK
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA