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The map label for this gene is nadE [H]

Identifier: 150396775

GI number: 150396775

Start: 1660932

End: 1662614

Strand: Reverse

Name: nadE [H]

Synonym: Smed_1566

Alternate gene names: 150396775

Gene position: 1662614-1660932 (Counterclockwise)

Preceding gene: 150396778

Following gene: 150396774

Centisome position: 43.96

GC content: 61.5

Gene sequence:

>1683_bases
ATGACTGCACAGAAAGCCGCTCCCTCAACGCTACGGATCGCCGTAGCACAGCTCAATCCAACAGTCGGAGACATTGCCGG
CAATATGACAAAGGCGCGCGAGGCGCGGGCGGCGGCGGCGCGCGAGGGCGCCGACCTGCTGCTCTTTACCGAGCTCTTCC
TGTCCGGCTACCCGCCCGAAGACCTTGTTCTGAAGCCCGCCTTCCTGAGCGCTTGCGAACAGGCCGTAGAGAAGCTCGCC
GCAGAGACGGCAGATGGTGGGCCGGGCATCGTTATCGGCTTCCCGAGACAGGCGGCCGCCCTCCGGCACAATTCCGTGGC
GGTGCTCGATGGCGGCAAGATCATCGCCGTCCGCGACAAGGTGGACTTGCCGAACTACGGCGAGTTTGACGAGAAACGCG
TCTTCGATGCGGGCGAAATGCCCGGGCCGGTGAACTTCCGCGGCGTGCGCATCGGTATACCCGTATGCGAGGACATATGG
GGCGATCTCGGCGTTTGCGAGACCCTTGCGGAGAGTGGCGCAGAGATTCTTCTTTCACCGAACGGCTCGCCCTATTATCG
CGGCAAGCTGGACGTGCGTCATCAGGTGGTCCTGAGGCAAGTGATCGAAACCGGGCTGCCGATGATCTATGCCAACCAGC
TCGGCGGTCAGGACGAACTGGTTTTCGACGGCGCGAGCTTTGCCTTCAACGCGGACAAGTCACTGGCCTTCCAGATGAGC
CAGTTCGAGGAGGCCGTCGCCGTATCCGAATGGAGAAGGGGTGCGGATGGCTGGGTCTCCGGCAACGGATTGAAGTCACG
CGTACCGGAAGGAGAGGAAGCGGATTATCGCGCCTGCATGCTGGGCTTCCGGGATTACGTCAACAAGAACGGTTTTCGTA
ATGTTGTGCTTGGCCTTTCCGGCGGGATCGATTCGGCAATCTGCACTGCACTTGCGGTGGATGCGTTGGGCGAGGAGCGG
GTCCGCACTGTCATGCTGCCATACCGCTACACCTCGCGCGAGTCGTTGCAGGATGCCGAGGCATGCGCCAGGGCCCTTGG
CTGCCGCTACGACATCGTTGCTATCGAGGAGCCCGTCGAGGGCTTTCTCAGCGCGCTCTCCGATATGTTCGAGGGTACCG
AAGCGGGCATTACCGAGGAGAACCTTCAGAGCCGCACGCGCGGGACGATCCTGATGGCGATATCCAACAAGTTCGGCTCC
ATGGTGGTGACGACGGGTAACAAATCCGAGATGTCGGTCGGCTACGCCACGCTTTACGGGGACATGAACGGCGGTTTCAA
CCCGATCAAGGATCTGTACAAGATGCAGGTTTATGCGCTGGCACGCTGGCGCAATGGCACGGTGCCGCCGGGAGCGCTGG
GGCCTTCGGGTGAAGTCATCCCGCAGAACATCATCGACAAGGCGCCGTCAGCCGAACTCCGTCCCGACCAGACGGACCAG
GACTCGCTGCCGCCTTATCCGGTGCTCGACGACATTCTCGAATGCCTGGTCGAGAAGGAGATGAGCACGGAGGATGTCGT
CGCACGGGGACATGAGGAGGCGACGGTCCACAGGATCGAGCATCTGCTCTACATTGCGGAATACAAGCGTCGGCAATCGG
CCCCAGGTGTGAAGATCACCAGGAAGAACTTCGGCCGCGATCGGCGCTATCCCATTACCAACCGGTTCCGCGACAGGGGA
TAA

Upstream 100 bases:

>100_bases
CCTTCTGAAATGGATTGAGTCTGGGTTTTTTGCCGCGGGATGCGGTAAGAACAACGCGAACGACACATGCATTCGCTGAC
GAGAGACGACGGGCCCATTC

Downstream 100 bases:

>100_bases
TCATGCGCAGCTCGGTGGAGGTCTTCGACGTGGCGACGGGCGAGGCGCGTGTCGTTTGGCAGACGGAACGGCTGGTCGAG
GCACCGAACTGGTCGCCGGA

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MTAQKAAPSTLRIAVAQLNPTVGDIAGNMTKAREARAAAAREGADLLLFTELFLSGYPPEDLVLKPAFLSACEQAVEKLA
AETADGGPGIVIGFPRQAAALRHNSVAVLDGGKIIAVRDKVDLPNYGEFDEKRVFDAGEMPGPVNFRGVRIGIPVCEDIW
GDLGVCETLAESGAEILLSPNGSPYYRGKLDVRHQVVLRQVIETGLPMIYANQLGGQDELVFDGASFAFNADKSLAFQMS
QFEEAVAVSEWRRGADGWVSGNGLKSRVPEGEEADYRACMLGFRDYVNKNGFRNVVLGLSGGIDSAICTALAVDALGEER
VRTVMLPYRYTSRESLQDAEACARALGCRYDIVAIEEPVEGFLSALSDMFEGTEAGITEENLQSRTRGTILMAISNKFGS
MVVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYALARWRNGTVPPGALGPSGEVIPQNIIDKAPSAELRPDQTDQ
DSLPPYPVLDDILECLVEKEMSTEDVVARGHEEATVHRIEHLLYIAEYKRRQSAPGVKITRKNFGRDRRYPITNRFRDRG

Sequences:

>Translated_560_residues
MTAQKAAPSTLRIAVAQLNPTVGDIAGNMTKAREARAAAAREGADLLLFTELFLSGYPPEDLVLKPAFLSACEQAVEKLA
AETADGGPGIVIGFPRQAAALRHNSVAVLDGGKIIAVRDKVDLPNYGEFDEKRVFDAGEMPGPVNFRGVRIGIPVCEDIW
GDLGVCETLAESGAEILLSPNGSPYYRGKLDVRHQVVLRQVIETGLPMIYANQLGGQDELVFDGASFAFNADKSLAFQMS
QFEEAVAVSEWRRGADGWVSGNGLKSRVPEGEEADYRACMLGFRDYVNKNGFRNVVLGLSGGIDSAICTALAVDALGEER
VRTVMLPYRYTSRESLQDAEACARALGCRYDIVAIEEPVEGFLSALSDMFEGTEAGITEENLQSRTRGTILMAISNKFGS
MVVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYALARWRNGTVPPGALGPSGEVIPQNIIDKAPSAELRPDQTDQ
DSLPPYPVLDDILECLVEKEMSTEDVVARGHEEATVHRIEHLLYIAEYKRRQSAPGVKITRKNFGRDRRYPITNRFRDRG
>Mature_559_residues
TAQKAAPSTLRIAVAQLNPTVGDIAGNMTKAREARAAAAREGADLLLFTELFLSGYPPEDLVLKPAFLSACEQAVEKLAA
ETADGGPGIVIGFPRQAAALRHNSVAVLDGGKIIAVRDKVDLPNYGEFDEKRVFDAGEMPGPVNFRGVRIGIPVCEDIWG
DLGVCETLAESGAEILLSPNGSPYYRGKLDVRHQVVLRQVIETGLPMIYANQLGGQDELVFDGASFAFNADKSLAFQMSQ
FEEAVAVSEWRRGADGWVSGNGLKSRVPEGEEADYRACMLGFRDYVNKNGFRNVVLGLSGGIDSAICTALAVDALGEERV
RTVMLPYRYTSRESLQDAEACARALGCRYDIVAIEEPVEGFLSALSDMFEGTEAGITEENLQSRTRGTILMAISNKFGSM
VVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYALARWRNGTVPPGALGPSGEVIPQNIIDKAPSAELRPDQTDQD
SLPPYPVLDDILECLVEKEMSTEDVVARGHEEATVHRIEHLLYIAEYKRRQSAPGVKITRKNFGRDRRYPITNRFRDRG

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17538756, Length=261, Percent_Identity=28.735632183908, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61033; Mature: 60902

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQKAAPSTLRIAVAQLNPTVGDIAGNMTKAREARAAAAREGADLLLFTELFLSGYPPE
CCCCCCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCH
DLVLKPAFLSACEQAVEKLAAETADGGPGIVIGFPRQAAALRHNSVAVLDGGKIIAVRDK
HCEECHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCEEEEECCEEEEEECC
VDLPNYGEFDEKRVFDAGEMPGPVNFRGVRIGIPVCEDIWGDLGVCETLAESGAEILLSP
CCCCCCCCCCHHHCCCCCCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHCCCEEEECC
NGSPYYRGKLDVRHQVVLRQVIETGLPMIYANQLGGQDELVFDGASFAFNADKSLAFQMS
CCCCEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECCCCEEECCCCHHHHHHH
QFEEAVAVSEWRRGADGWVSGNGLKSRVPEGEEADYRACMLGFRDYVNKNGFRNVVLGLS
HHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEC
GGIDSAICTALAVDALGEERVRTVMLPYRYTSRESLQDAEACARALGCRYDIVAIEEPVE
CCCHHHHHHHHHHHHCCCHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHH
GFLSALSDMFEGTEAGITEENLQSRTRGTILMAISNKFGSMVVTTGNKSEMSVGYATLYG
HHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCEEEEEEEEE
DMNGGFNPIKDLYKMQVYALARWRNGTVPPGALGPSGEVIPQNIIDKAPSAELRPDQTDQ
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCC
DSLPPYPVLDDILECLVEKEMSTEDVVARGHEEATVHRIEHLLYIAEYKRRQSAPGVKIT
CCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
RKNFGRDRRYPITNRFRDRG
HHHCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
TAQKAAPSTLRIAVAQLNPTVGDIAGNMTKAREARAAAAREGADLLLFTELFLSGYPPE
CCCCCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCH
DLVLKPAFLSACEQAVEKLAAETADGGPGIVIGFPRQAAALRHNSVAVLDGGKIIAVRDK
HCEECHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCEEEEECCEEEEEECC
VDLPNYGEFDEKRVFDAGEMPGPVNFRGVRIGIPVCEDIWGDLGVCETLAESGAEILLSP
CCCCCCCCCCHHHCCCCCCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHCCCEEEECC
NGSPYYRGKLDVRHQVVLRQVIETGLPMIYANQLGGQDELVFDGASFAFNADKSLAFQMS
CCCCEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECCCCEEECCCCHHHHHHH
QFEEAVAVSEWRRGADGWVSGNGLKSRVPEGEEADYRACMLGFRDYVNKNGFRNVVLGLS
HHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEC
GGIDSAICTALAVDALGEERVRTVMLPYRYTSRESLQDAEACARALGCRYDIVAIEEPVE
CCCHHHHHHHHHHHHCCCHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHH
GFLSALSDMFEGTEAGITEENLQSRTRGTILMAISNKFGSMVVTTGNKSEMSVGYATLYG
HHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCEEEEEEEEE
DMNGGFNPIKDLYKMQVYALARWRNGTVPPGALGPSGEVIPQNIIDKAPSAELRPDQTDQ
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCC
DSLPPYPVLDDILECLVEKEMSTEDVVARGHEEATVHRIEHLLYIAEYKRRQSAPGVKIT
CCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
RKNFGRDRRYPITNRFRDRG
HHHCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]