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The map label for this gene is purB [H]

Identifier: 150396705

GI number: 150396705

Start: 1577099

End: 1578406

Strand: Direct

Name: purB [H]

Synonym: Smed_1492

Alternate gene names: 150396705

Gene position: 1577099-1578406 (Clockwise)

Preceding gene: 150396704

Following gene: 150396706

Centisome position: 41.7

GC content: 59.48

Gene sequence:

>1308_bases
ATGATCCCCCGTTACTCCCGGCCGGAAATGGTGGCCATCTGGTCGCCGGAAACCAAATTCCGAATTTGGTTCGAGATCGA
GGCGCATGCCTGCGATGCCTTGGCCGAAATCGGCGTGATTCCCAAGGAGGCCGCGAAGACCATCTGGGAAAAGGGTGGCG
CCGCCAAGTTCGACGTCGATCGCATCGACGAGATCGAAGCGGTCACCAAGCACGATGTCATTGCCTTCCTGACCCATCTC
GCCGAATTCGTCGGTCCCGACAGCCGCTTCATTCACCAAGGCATGACCTCGTCCGACGTTCTGGATACCTGCTTCAACGT
TCAACTCGTCCGCGCGACGGATCTCATGCTCGCCGATCTTGACAAGTTGCTCGAAGCGCTGAAGCGGCGTGCCTTCGAAC
ACAAGGACACGGTGACGATCGGCCGTTCTCACGGTATCCACGCCGAGCCGACCACCTTTGGTGTCAAACTTGCACTGGCC
TATGCGGAGTTCGACCGGTGCAAGCAACGCCTTCTCGCCGCCCGCGAGGAAATTGCAACCTGTGCGATTTCCGGAGCAGT
GGGCACCTTCGCCAATATCGATCCAAGGGTCGAGGAGCACGTTGCAAAGGCGCTTGGCCTCAAGCCTGAGCCCGTCTCGA
CCCAGGTGATCCCGCGTGATCGCCATGCGATGTATTTCGCGACGCTGGGCGTCATCGGCTCCTCGATCGAGCGTCTCGCA
ACCGAGATCCGGCATTTGCAGCGCACGGAGGTCCTGGAGGCGGAAGAGTATTTCTCTCCGGGTCAGAAGGGCTCTTCGGC
GATGCCGCACAAACGTAACCCGGTGCTGACGGAAAACCTGACCGGTCTTGCCCGCATGGTTCGAGCCTTTGTCGTTCCGG
CCATGGAGAACGTCGCGCTATGGCACGAACGCGATATCTCGCATTCCTCCGTCGAGCGCATGATCGGCCCGGATGCGACA
GTCACGCTTGATTTCGCTCTGGCGCGCCTCACGAGCGTGATCGACAAGCTTCTTGTCTACCCGGAGAACATGCTGAAGAA
TATGAACAAGTTCCGCGGGCTTGTTCATTCGCAGCGCGTTCTCCTGGCCCTCACGCAGGCAGGCGTGTCACGCGAGGACG
CCTATCGGCTCGTCCAGCGCAACGCCATGAAAGTGTGGGAGCAGGGTAAAGACTTCCTCGAAGAACTGCTCGCCGACACT
GAAGTGCGCGCGGCACTCTCCGAAGAGGAAATCCGTGAGAAGTTCGACCTCGGCTATCACACCAAGCATGTCGACACGAT
CTTTCAGAGGGTCTTCGGCGCCGTTTAG

Upstream 100 bases:

>100_bases
CTGCTCGCGCGATCGGACGCACGGCAACGGCGTTGAGCTTAGCCGCCATCTTTGATAGAGGCACTGCCATCCCTCCACTC
TCGACGAAAGCTTGAAGCCC

Downstream 100 bases:

>100_bases
AGCGTTCTGTTTTAGCGCTGGTTTTCGCCCGCATCTGACATCCGATGTTCAAATGCGGGCGAAGTCTTTAGCAGTCCTTT
AGGTGCTCCATGAGAAGATC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAEIGVIPKEAAKTIWEKGGAAKFDVDRIDEIEAVTKHDVIAFLTHL
AEFVGPDSRFIHQGMTSSDVLDTCFNVQLVRATDLMLADLDKLLEALKRRAFEHKDTVTIGRSHGIHAEPTTFGVKLALA
YAEFDRCKQRLLAAREEIATCAISGAVGTFANIDPRVEEHVAKALGLKPEPVSTQVIPRDRHAMYFATLGVIGSSIERLA
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRAFVVPAMENVALWHERDISHSSVERMIGPDAT
VTLDFALARLTSVIDKLLVYPENMLKNMNKFRGLVHSQRVLLALTQAGVSREDAYRLVQRNAMKVWEQGKDFLEELLADT
EVRAALSEEEIREKFDLGYHTKHVDTIFQRVFGAV

Sequences:

>Translated_435_residues
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAEIGVIPKEAAKTIWEKGGAAKFDVDRIDEIEAVTKHDVIAFLTHL
AEFVGPDSRFIHQGMTSSDVLDTCFNVQLVRATDLMLADLDKLLEALKRRAFEHKDTVTIGRSHGIHAEPTTFGVKLALA
YAEFDRCKQRLLAAREEIATCAISGAVGTFANIDPRVEEHVAKALGLKPEPVSTQVIPRDRHAMYFATLGVIGSSIERLA
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRAFVVPAMENVALWHERDISHSSVERMIGPDAT
VTLDFALARLTSVIDKLLVYPENMLKNMNKFRGLVHSQRVLLALTQAGVSREDAYRLVQRNAMKVWEQGKDFLEELLADT
EVRAALSEEEIREKFDLGYHTKHVDTIFQRVFGAV
>Mature_435_residues
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAEIGVIPKEAAKTIWEKGGAAKFDVDRIDEIEAVTKHDVIAFLTHL
AEFVGPDSRFIHQGMTSSDVLDTCFNVQLVRATDLMLADLDKLLEALKRRAFEHKDTVTIGRSHGIHAEPTTFGVKLALA
YAEFDRCKQRLLAAREEIATCAISGAVGTFANIDPRVEEHVAKALGLKPEPVSTQVIPRDRHAMYFATLGVIGSSIERLA
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRAFVVPAMENVALWHERDISHSSVERMIGPDAT
VTLDFALARLTSVIDKLLVYPENMLKNMNKFRGLVHSQRVLLALTQAGVSREDAYRLVQRNAMKVWEQGKDFLEELLADT
EVRAALSEEEIREKFDLGYHTKHVDTIFQRVFGAV

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=420, Percent_Identity=28.0952380952381, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=28.0839895013123, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1787376, Length=362, Percent_Identity=29.5580110497238, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=401, Percent_Identity=26.4339152119701, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32564234, Length=368, Percent_Identity=27.445652173913, Blast_Score=95, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6323391, Length=416, Percent_Identity=27.4038461538462, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24647570, Length=404, Percent_Identity=26.7326732673267, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48897; Mature: 48897

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAEIGVIPKEAAKTIWEKGGAAKFDVD
CCCCCCCCCEEEEECCCCEEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHH
RIDEIEAVTKHDVIAFLTHLAEFVGPDSRFIHQGMTSSDVLDTCFNVQLVRATDLMLADL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCEEEEHHHHHHHHHH
DKLLEALKRRAFEHKDTVTIGRSHGIHAEPTTFGVKLALAYAEFDRCKQRLLAAREEIAT
HHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANIDPRVEEHVAKALGLKPEPVSTQVIPRDRHAMYFATLGVIGSSIERLA
HHHHCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRAFVVPAMENVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERMIGPDATVTLDFALARLTSVIDKLLVYPENMLKNMNKFRGLVHSQRV
HHHCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LLALTQAGVSREDAYRLVQRNAMKVWEQGKDFLEELLADTEVRAALSEEEIREKFDLGYH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TKHVDTIFQRVFGAV
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAEIGVIPKEAAKTIWEKGGAAKFDVD
CCCCCCCCCEEEEECCCCEEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHH
RIDEIEAVTKHDVIAFLTHLAEFVGPDSRFIHQGMTSSDVLDTCFNVQLVRATDLMLADL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCEEEEHHHHHHHHHH
DKLLEALKRRAFEHKDTVTIGRSHGIHAEPTTFGVKLALAYAEFDRCKQRLLAAREEIAT
HHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANIDPRVEEHVAKALGLKPEPVSTQVIPRDRHAMYFATLGVIGSSIERLA
HHHHCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRAFVVPAMENVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERMIGPDATVTLDFALARLTSVIDKLLVYPENMLKNMNKFRGLVHSQRV
HHHCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LLALTQAGVSREDAYRLVQRNAMKVWEQGKDFLEELLADTEVRAALSEEEIREKFDLGYH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TKHVDTIFQRVFGAV
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]