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The map label for this gene is livJ [C]

Identifier: 150396700

GI number: 150396700

Start: 1572796

End: 1573884

Strand: Direct

Name: livJ [C]

Synonym: Smed_1487

Alternate gene names: 150396700

Gene position: 1572796-1573884 (Clockwise)

Preceding gene: 150396692

Following gene: 150396701

Centisome position: 41.59

GC content: 61.71

Gene sequence:

>1089_bases
ATGCGGAAACCAATCGCCACCAGCATCCTAGTCGCCGCGCTCACGCTGAGCGGCCCTGCTTTCGCCGCCGGCGTAAAAAT
CGCCGTCGTAGCGCCCGCAGAAGGGCCCTTCGCCGCGTTGGGCAAGCAGATTTCTGCCGGAGCAGCATTCCAGGCCGGCG
ATCGTGGAAGCCAGGTAATCCCGATCAACGAAAGCTGTGATCCAGCGGGCAGCGATGCGTTGACGAAAGCTCTGCTTGCG
AGCGGCGCCGAAGCCGCGGTCGGATTTCTTTGCACCGAAAGTCTGGAAGCCGCGCTGCCGGCGCTCGCCGGAGCCGGCAT
TCCGGCCATTACCGTCAGCGTCCGCTCGGATATCCTCATGGAGGACGCGCTTAAGAAAAACTGGCCGTTCTACCGCCTTG
CGCCCAGTGGAAAGTCGGAAGCGGCAGCGGTCACCGATGCCATCGTCTCCAACTGGAAAGGCAGGCCACTCGCACTCATA
GATGACGGGACGATCCAAAGTCGCGAACTGGTGGAAAGTGTACGCAACGGCTTGGCCGAGATCGGCGTCACCCCCGTTTT
CACCGACACCTACCGCCCTTCGCAGGAACAGCAGGTGAGCCTCGTGCGCAGACTGGCGAAGAGCGGTGCAACGCATGTCT
TCACCGGAGGTGACCGTAACGACACCGCGGTGATTGCGCGCGACGCGAAGGCGGAAAATGTTCCAATTACCCTTCTCGGC
GGCGATACACTGAAAGCCGCCGATCTCGACGTGCCGCTGGAACACGGCGTTCTCGCCATGACCATACCTGATCCCGCCCG
TTCTGCGGAAGCCGCGCCGGTAGCGAAGGCGATGCGTGCAGCCGGGATCGAGCCGGAAGGCTATGTGCTTCCAGCCTTTG
CCGCGGTGTCCCTGCTCGAACAGGCGAAGGACCAGGCAGAAAAAGATGACAACACGCTCGCCGTTGCTCTGGCGAAAGGG
TCCTATACGACAGTCCTCGGGCCGATAGGGTTTACTCGCTCGCACGAGCTCGCCGAAACTCCCTTCAGGCTTATGCGATG
GCAGGATGGACACTTCGTCGATGCAGCCAATAATGGCACGACCGAATGA

Upstream 100 bases:

>100_bases
CTGGGAACAAGTCAGCGACAATGCCTGCAATGCCTCTATCTTTTCCTTTGACGAGCGCGACGACATCCTTAAAACATGGC
GCTCGATAGCGGGAAAAAAC

Downstream 100 bases:

>100_bases
TGCAGAAGGGCGCCAGAAATCTGATCACGGACGTTCCGGGCTTGCTGGTGGGCAATGCTGAAGACCATCACCTGAAATCC
GGTGTCACGACGGTGCTCTG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MRKPIATSILVAALTLSGPAFAAGVKIAVVAPAEGPFAALGKQISAGAAFQAGDRGSQVIPINESCDPAGSDALTKALLA
SGAEAAVGFLCTESLEAALPALAGAGIPAITVSVRSDILMEDALKKNWPFYRLAPSGKSEAAAVTDAIVSNWKGRPLALI
DDGTIQSRELVESVRNGLAEIGVTPVFTDTYRPSQEQQVSLVRRLAKSGATHVFTGGDRNDTAVIARDAKAENVPITLLG
GDTLKAADLDVPLEHGVLAMTIPDPARSAEAAPVAKAMRAAGIEPEGYVLPAFAAVSLLEQAKDQAEKDDNTLAVALAKG
SYTTVLGPIGFTRSHELAETPFRLMRWQDGHFVDAANNGTTE

Sequences:

>Translated_362_residues
MRKPIATSILVAALTLSGPAFAAGVKIAVVAPAEGPFAALGKQISAGAAFQAGDRGSQVIPINESCDPAGSDALTKALLA
SGAEAAVGFLCTESLEAALPALAGAGIPAITVSVRSDILMEDALKKNWPFYRLAPSGKSEAAAVTDAIVSNWKGRPLALI
DDGTIQSRELVESVRNGLAEIGVTPVFTDTYRPSQEQQVSLVRRLAKSGATHVFTGGDRNDTAVIARDAKAENVPITLLG
GDTLKAADLDVPLEHGVLAMTIPDPARSAEAAPVAKAMRAAGIEPEGYVLPAFAAVSLLEQAKDQAEKDDNTLAVALAKG
SYTTVLGPIGFTRSHELAETPFRLMRWQDGHFVDAANNGTTE
>Mature_362_residues
MRKPIATSILVAALTLSGPAFAAGVKIAVVAPAEGPFAALGKQISAGAAFQAGDRGSQVIPINESCDPAGSDALTKALLA
SGAEAAVGFLCTESLEAALPALAGAGIPAITVSVRSDILMEDALKKNWPFYRLAPSGKSEAAAVTDAIVSNWKGRPLALI
DDGTIQSRELVESVRNGLAEIGVTPVFTDTYRPSQEQQVSLVRRLAKSGATHVFTGGDRNDTAVIARDAKAENVPITLLG
GDTLKAADLDVPLEHGVLAMTIPDPARSAEAAPVAKAMRAAGIEPEGYVLPAFAAVSLLEQAKDQAEKDDNTLAVALAKG
SYTTVLGPIGFTRSHELAETPFRLMRWQDGHFVDAANNGTTE

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=349, Percent_Identity=23.4957020057307, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789867, Length=350, Percent_Identity=23.7142857142857, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 37473; Mature: 37473

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPIATSILVAALTLSGPAFAAGVKIAVVAPAEGPFAALGKQISAGAAFQAGDRGSQVI
CCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHCCHHCCCCCEECCCCCCEEE
PINESCDPAGSDALTKALLASGAEAAVGFLCTESLEAALPALAGAGIPAITVSVRSDILM
ECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHH
EDALKKNWPFYRLAPSGKSEAAAVTDAIVSNWKGRPLALIDDGTIQSRELVESVRNGLAE
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
IGVTPVFTDTYRPSQEQQVSLVRRLAKSGATHVFTGGDRNDTAVIARDAKAENVPITLLG
HCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCCEEEEC
GDTLKAADLDVPLEHGVLAMTIPDPARSAEAAPVAKAMRAAGIEPEGYVLPAFAAVSLLE
CCCEEECCCCCCHHCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHH
QAKDQAEKDDNTLAVALAKGSYTTVLGPIGFTRSHELAETPFRLMRWQDGHFVDAANNGT
HHHHHHCCCCCEEEEEEECCCCEEEEECCCCCCCCHHHHCHHHHHCCCCCCEEECCCCCC
TE
CC
>Mature Secondary Structure
MRKPIATSILVAALTLSGPAFAAGVKIAVVAPAEGPFAALGKQISAGAAFQAGDRGSQVI
CCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHCCHHCCCCCEECCCCCCEEE
PINESCDPAGSDALTKALLASGAEAAVGFLCTESLEAALPALAGAGIPAITVSVRSDILM
ECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHH
EDALKKNWPFYRLAPSGKSEAAAVTDAIVSNWKGRPLALIDDGTIQSRELVESVRNGLAE
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
IGVTPVFTDTYRPSQEQQVSLVRRLAKSGATHVFTGGDRNDTAVIARDAKAENVPITLLG
HCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCCEEEEC
GDTLKAADLDVPLEHGVLAMTIPDPARSAEAAPVAKAMRAAGIEPEGYVLPAFAAVSLLE
CCCEEECCCCCCHHCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHH
QAKDQAEKDDNTLAVALAKGSYTTVLGPIGFTRSHELAETPFRLMRWQDGHFVDAANNGT
HHHHHHCCCCCEEEEEEECCCCEEEEECCCCCCCCHHHHCHHHHHCCCCCCEEECCCCCC
TE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA