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The map label for this gene is tyrS [H]

Identifier: 150396686

GI number: 150396686

Start: 1556778

End: 1558031

Strand: Direct

Name: tyrS [H]

Synonym: Smed_1473

Alternate gene names: 150396686

Gene position: 1556778-1558031 (Clockwise)

Preceding gene: 150396683

Following gene: 150396688

Centisome position: 41.16

GC content: 60.37

Gene sequence:

>1254_bases
ATGTCCGAGTTCAAGTCCGATTTCCTGCACACCCTCAGCGAACGCGGTTTCATCCATCAGACGTCGGATAATGGCGGTCT
CGATCAGCTGTTCCTGACGGAAACCGTGACTGCATATATCGGCTTCGATCCTACGGCTGCGAGCCTGCATGCAGGCGGCC
TTATCCAGATCATGATGCTGCATTGGCTGCAGGCGACCGGACACCGCCCCATTTCGCTGATGGGCGGCGGCACAGGCATG
GTTGGCGATCCGTCCTTCAAGGACGAGGCGCGGCAACTGATGACGCCTGAAACGATCGCCGCCAATATAGCGAGCATCAA
GACTGTCTTCTCGAACTATCTGAAATACGGCGACGGCCCCAAGGACGCGCTGATGATCAACAATGCGGACTGGCTGCTCG
GTATCAACTATCTCGAATTCCTGCGTGATGTCGGCCGGCATTTCTCGGTGAACCGCATGCTGTCCTTCGACAGCGTGAAG
ATGAGGCTCGAGCGCGAGCAGTCGCTCTCTTTCCTCGAATTCAACTACATGATCCTACAGGCCTACGACTTCGTCGAGCT
CTACCAGCGCACTGGCTGCCGGCTCCAGATGGGCGGTTCGGATCAGTGGGGCAATATCGTCAACGGCATCGATCTCGGCC
ATCGCATGGGAACGCCGCAGCTCTACGCGCTCACCTCGCCGCTGTTGACCACTGCCTCCGGCGCAAAGATGGGCAAATCG
CTGAGCGGCGCCGTCTGGCTCAATCCGGATATGCTCGGTCCCTACGAGTTCTGGCAATACTGGCGAAACACGGAGGATGC
AGACGTCACGCGCTTCCTCAAGCTCTACACCACGCTGCCGATGGCTGAGATCGACCGACTGTCGAAGCTCGGCGGCTCCG
AGATCAACGAGGTTAAGAAGGTGCTGGCAACCGAGGTCACTGCCATGCTGCACGGGCGCACGGCGGCGGAACAGGCCGCC
GAGACGGCGCGCAAGACCTTTGAGGAAGGTGCGCTTGCCGAGAACCTGCCCTCGATCGAAGTACCCGCTCGAGAATTCGA
ATCGGGGCTCGGGCTGCTTGCATTGATGGTACGTGCCGGTCTTGCCGCTTCGAACGGCGAAGCTCGGCGCCATGTTCAGG
GCGGCGCCGTCCGGATCAACGACGATCAGGTCACCGATGAGCGCCGTGTCGTCGGAAGCCGTGACGTGTCCGGGGACGGC
GTGATCAAGCTCTCGCTGGGCAAGAAGAAGCATCTGCTCGTCCGGCCCGTGTGA

Upstream 100 bases:

>100_bases
GTTCATAGCAAAAAGGGTCGCCAAGCAAAAACGTGCACTTTCGACGAAACAGTGATAAAGGCGCGGCGCACACGTTCTAC
CCAGACGAAAGAGACAGCTT

Downstream 100 bases:

>100_bases
ATCCGAGGCCGGCATGAGATCGTAATGTTTCGGGTCGGCCTCTCATTGAAACTCGAAGATATTGCGGAAGACGCCCGGCG
CTATGATCGACAGCGGGTTG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSEFKSDFLHTLSERGFIHQTSDNGGLDQLFLTETVTAYIGFDPTAASLHAGGLIQIMMLHWLQATGHRPISLMGGGTGM
VGDPSFKDEARQLMTPETIAANIASIKTVFSNYLKYGDGPKDALMINNADWLLGINYLEFLRDVGRHFSVNRMLSFDSVK
MRLEREQSLSFLEFNYMILQAYDFVELYQRTGCRLQMGGSDQWGNIVNGIDLGHRMGTPQLYALTSPLLTTASGAKMGKS
LSGAVWLNPDMLGPYEFWQYWRNTEDADVTRFLKLYTTLPMAEIDRLSKLGGSEINEVKKVLATEVTAMLHGRTAAEQAA
ETARKTFEEGALAENLPSIEVPAREFESGLGLLALMVRAGLAASNGEARRHVQGGAVRINDDQVTDERRVVGSRDVSGDG
VIKLSLGKKKHLLVRPV

Sequences:

>Translated_417_residues
MSEFKSDFLHTLSERGFIHQTSDNGGLDQLFLTETVTAYIGFDPTAASLHAGGLIQIMMLHWLQATGHRPISLMGGGTGM
VGDPSFKDEARQLMTPETIAANIASIKTVFSNYLKYGDGPKDALMINNADWLLGINYLEFLRDVGRHFSVNRMLSFDSVK
MRLEREQSLSFLEFNYMILQAYDFVELYQRTGCRLQMGGSDQWGNIVNGIDLGHRMGTPQLYALTSPLLTTASGAKMGKS
LSGAVWLNPDMLGPYEFWQYWRNTEDADVTRFLKLYTTLPMAEIDRLSKLGGSEINEVKKVLATEVTAMLHGRTAAEQAA
ETARKTFEEGALAENLPSIEVPAREFESGLGLLALMVRAGLAASNGEARRHVQGGAVRINDDQVTDERRVVGSRDVSGDG
VIKLSLGKKKHLLVRPV
>Mature_416_residues
SEFKSDFLHTLSERGFIHQTSDNGGLDQLFLTETVTAYIGFDPTAASLHAGGLIQIMMLHWLQATGHRPISLMGGGTGMV
GDPSFKDEARQLMTPETIAANIASIKTVFSNYLKYGDGPKDALMINNADWLLGINYLEFLRDVGRHFSVNRMLSFDSVKM
RLEREQSLSFLEFNYMILQAYDFVELYQRTGCRLQMGGSDQWGNIVNGIDLGHRMGTPQLYALTSPLLTTASGAKMGKSL
SGAVWLNPDMLGPYEFWQYWRNTEDADVTRFLKLYTTLPMAEIDRLSKLGGSEINEVKKVLATEVTAMLHGRTAAEQAAE
TARKTFEEGALAENLPSIEVPAREFESGLGLLALMVRAGLAASNGEARRHVQGGAVRINDDQVTDERRVVGSRDVSGDGV
IKLSLGKKKHLLVRPV

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=425, Percent_Identity=34.1176470588235, Blast_Score=227, Evalue=2e-59,
Organism=Escherichia coli, GI1787925, Length=419, Percent_Identity=40.3341288782816, Blast_Score=319, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI25145802, Length=398, Percent_Identity=31.6582914572864, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25145805, Length=327, Percent_Identity=29.3577981651376, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325160, Length=444, Percent_Identity=34.009009009009, Blast_Score=228, Evalue=1e-60,
Organism=Drosophila melanogaster, GI20130383, Length=432, Percent_Identity=34.4907407407407, Blast_Score=244, Evalue=8e-65,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46045; Mature: 45914

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFKSDFLHTLSERGFIHQTSDNGGLDQLFLTETVTAYIGFDPTAASLHAGGLIQIMML
CCHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHH
HWLQATGHRPISLMGGGTGMVGDPSFKDEARQLMTPETIAANIASIKTVFSNYLKYGDGP
HHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
KDALMINNADWLLGINYLEFLRDVGRHFSVNRMLSFDSVKMRLEREQSLSFLEFNYMILQ
CCEEEECCCCCEEECHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHH
AYDFVELYQRTGCRLQMGGSDQWGNIVNGIDLGHRMGTPQLYALTSPLLTTASGAKMGKS
HHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHCCCCHHHCCC
LSGAVWLNPDMLGPYEFWQYWRNTEDADVTRFLKLYTTLPMAEIDRLSKLGGSEINEVKK
CCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHH
VLATEVTAMLHGRTAAEQAAETARKTFEEGALAENLPSIEVPAREFESGLGLLALMVRAG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHCCHHHHHHHHHHC
LAASNGEARRHVQGGAVRINDDQVTDERRVVGSRDVSGDGVIKLSLGKKKHLLVRPV
CCCCCCCHHHHCCCCEEEECCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCEEEECC
>Mature Secondary Structure 
SEFKSDFLHTLSERGFIHQTSDNGGLDQLFLTETVTAYIGFDPTAASLHAGGLIQIMML
CHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHH
HWLQATGHRPISLMGGGTGMVGDPSFKDEARQLMTPETIAANIASIKTVFSNYLKYGDGP
HHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
KDALMINNADWLLGINYLEFLRDVGRHFSVNRMLSFDSVKMRLEREQSLSFLEFNYMILQ
CCEEEECCCCCEEECHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHH
AYDFVELYQRTGCRLQMGGSDQWGNIVNGIDLGHRMGTPQLYALTSPLLTTASGAKMGKS
HHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHCCCCHHHCCC
LSGAVWLNPDMLGPYEFWQYWRNTEDADVTRFLKLYTTLPMAEIDRLSKLGGSEINEVKK
CCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHH
VLATEVTAMLHGRTAAEQAAETARKTFEEGALAENLPSIEVPAREFESGLGLLALMVRAG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHCCHHHHHHHHHHC
LAASNGEARRHVQGGAVRINDDQVTDERRVVGSRDVSGDGVIKLSLGKKKHLLVRPV
CCCCCCCHHHHCCCCEEEECCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]