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The map label for this gene is phbC [H]

Identifier: 150396669

GI number: 150396669

Start: 1537766

End: 1539601

Strand: Direct

Name: phbC [H]

Synonym: Smed_1456

Alternate gene names: 150396669

Gene position: 1537766-1539601 (Clockwise)

Preceding gene: 150396664

Following gene: 150396670

Centisome position: 40.66

GC content: 60.68

Gene sequence:

>1836_bases
GTGACAGCAGAGAAGGCCGAGGGCGCTAAGGGCTTTGCCGGCTTCGACCCGAAATCGGTCGAGCCCTACATCGTCAAGGA
CCCCGAAAACCTGGCCATCAACATGGCTCGCGCGGCCGAGCAGCTAGGAAAAGCTGCTTCCGCCTGGCTTGCGCCCCGCG
AATCCGGAGAAAAGACGGATAGCTTCGCCGAGCCGGTCTCCGATATGGTCAAAACGCTTTCCAAAGTCTCGGAATACTGG
CTCACAGACCCACGGCGGACACTCGAAGCCCAGACTCATCTTCTCGGCAGTTTCTTCGATATGTGGTCCCGGACGGTCCA
CCGGATGGCGGGCGACGCAGTCGACGAGCCGGCCAGCCTTCAGCGCAACGACAAGCGCTTCTCCGACGAAGACTGGGTGA
GGAATCCCTTTTTCGATTTCATACGTCAGGCCTATTTCGTTACGTCGGACTGGGCGGAGCGCATGGTGAACGACGCCGAG
GGCCTCGACGACCATACCCGGCACAAGGCCGCCTTCTATGTTCGCCAGATTGCCAGCGCCCTTTCCCCGACCAACTTCAT
CACCACCAACCCGCAGCTATATCGCGAAACAGTGGCGACCAGCGGCGCCAATCTCGTGAAGGGCATGCAGATGCTGGCGG
AGGACATCGCCGCCGGCCGCGGCGAGCTTCGCCTGCGGCAGACTGACACAAGCAAGTTCGCCATCGGCGAAAACATCGCG
GTTACGCCGGGAAAAGTCATCGCGCAGAACGATATTTGCCAGGTGCTGCAATACGAGGCCAGCACCGAAACCGTACTCAA
GCGGCCGTTGCTCATTTGCCCGCCCTGGATCAACAAATTCTACGTGTTGGATCTCAACCCCGAGAAATCCTTCATCAAAT
GGGCGGTCGACCAGGGCCACACGGTCTTCGTCATTTCCTGGGTGAACCCTGACGAGCGCCATGCAACGAAGGACTGGGAA
GCCTATGCGCGCGATGGCATCGGCTTCGCGCTGGACATCATCGAACAGGCAACCGAAGAGCGCGAAGTCAATTCCATCGG
CTATTGCGTCGGCGGCACGCTGCTCGCCGCCACCCTGGCGCTGCATGCCGCGGAAAGGGATGACCGCATTCGCTCCGCGA
CGCTCTTCACCACCCAGGTGGATTTCACCCATGCGGGCGATCTGAAGGTCTTCGTGGACGACGACCAGATCCGCCACCTC
GAGGCCAATATGAGCGCCACCGGATATCTGGAAGGCTCGAAGATGGCGTCCGCCTTCAACATGCTGAGGGCCTCGGAGCT
GATCTGGCCTTATTTCGTCAATAATTATCTCAAGGGTCAGGATCCCCTGCCCTTCGACCTGCTCTACTGGAATTCCGACT
CGACACGGATGCCCGCGGCCAACCACTCCTTCTATCTCCGCAATTGCTATCTGGAGAACAGGCTTGCCAAAGGCGAGATG
GTGCTTGCCGGTCGTCGGATATCCCTCGGCGACGTCAAGATCCCCGTCTACAATCTCGCGACGAAGGAGGATCACATCGC
GCCGGCAAAATCGGTGTTTCTCGGAAGCAGCAGCTTCGGCGGCAAGGTGACGTTCGTGCTCTCCGGCTCGGGGCACATCG
CGGGTGTGGTCAACCCCCCGGCTCGCGGCAAGTATCAATACTGGACGGGAGGGCCGCCCAAGGGCGACGTCGAAACCTGG
ATGAGCAAGGCAAAGGAAACGGCCGGCTCCTGGTGGCCGCATTGGCAAAGCTGGGTCGAACGGCTCGACAAACGCCGTGT
TCCGGCCCGCAAGGCCGGGGGTCCGCTCAATTCGATCGAGGAAGCTCCGGGCTCCTACGTGCGTATGCGCGCCTGA

Upstream 100 bases:

>100_bases
CACCTCTGTTTCCACGGCGCGACGATGCGCTAGCCCTTGTAATCATTTTCGGGCAGAATGTAAAAATACAAAACAAACAA
GATGCTGTGGAGGAAACCGG

Downstream 100 bases:

>100_bases
CCCGCACTTCTCCTGTAAGTGCTCTACTAGAAAGTCCCGTTCCTTGAAGTTCACCGGCCCGCTCGTCCCTGCGACGCTCG
TGCAGCGTTATAAGCGCTTC

Product: poly(R)-hydroxyalkanoic acid synthase, class I

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MTAEKAEGAKGFAGFDPKSVEPYIVKDPENLAINMARAAEQLGKAASAWLAPRESGEKTDSFAEPVSDMVKTLSKVSEYW
LTDPRRTLEAQTHLLGSFFDMWSRTVHRMAGDAVDEPASLQRNDKRFSDEDWVRNPFFDFIRQAYFVTSDWAERMVNDAE
GLDDHTRHKAAFYVRQIASALSPTNFITTNPQLYRETVATSGANLVKGMQMLAEDIAAGRGELRLRQTDTSKFAIGENIA
VTPGKVIAQNDICQVLQYEASTETVLKRPLLICPPWINKFYVLDLNPEKSFIKWAVDQGHTVFVISWVNPDERHATKDWE
AYARDGIGFALDIIEQATEEREVNSIGYCVGGTLLAATLALHAAERDDRIRSATLFTTQVDFTHAGDLKVFVDDDQIRHL
EANMSATGYLEGSKMASAFNMLRASELIWPYFVNNYLKGQDPLPFDLLYWNSDSTRMPAANHSFYLRNCYLENRLAKGEM
VLAGRRISLGDVKIPVYNLATKEDHIAPAKSVFLGSSSFGGKVTFVLSGSGHIAGVVNPPARGKYQYWTGGPPKGDVETW
MSKAKETAGSWWPHWQSWVERLDKRRVPARKAGGPLNSIEEAPGSYVRMRA

Sequences:

>Translated_611_residues
MTAEKAEGAKGFAGFDPKSVEPYIVKDPENLAINMARAAEQLGKAASAWLAPRESGEKTDSFAEPVSDMVKTLSKVSEYW
LTDPRRTLEAQTHLLGSFFDMWSRTVHRMAGDAVDEPASLQRNDKRFSDEDWVRNPFFDFIRQAYFVTSDWAERMVNDAE
GLDDHTRHKAAFYVRQIASALSPTNFITTNPQLYRETVATSGANLVKGMQMLAEDIAAGRGELRLRQTDTSKFAIGENIA
VTPGKVIAQNDICQVLQYEASTETVLKRPLLICPPWINKFYVLDLNPEKSFIKWAVDQGHTVFVISWVNPDERHATKDWE
AYARDGIGFALDIIEQATEEREVNSIGYCVGGTLLAATLALHAAERDDRIRSATLFTTQVDFTHAGDLKVFVDDDQIRHL
EANMSATGYLEGSKMASAFNMLRASELIWPYFVNNYLKGQDPLPFDLLYWNSDSTRMPAANHSFYLRNCYLENRLAKGEM
VLAGRRISLGDVKIPVYNLATKEDHIAPAKSVFLGSSSFGGKVTFVLSGSGHIAGVVNPPARGKYQYWTGGPPKGDVETW
MSKAKETAGSWWPHWQSWVERLDKRRVPARKAGGPLNSIEEAPGSYVRMRA
>Mature_610_residues
TAEKAEGAKGFAGFDPKSVEPYIVKDPENLAINMARAAEQLGKAASAWLAPRESGEKTDSFAEPVSDMVKTLSKVSEYWL
TDPRRTLEAQTHLLGSFFDMWSRTVHRMAGDAVDEPASLQRNDKRFSDEDWVRNPFFDFIRQAYFVTSDWAERMVNDAEG
LDDHTRHKAAFYVRQIASALSPTNFITTNPQLYRETVATSGANLVKGMQMLAEDIAAGRGELRLRQTDTSKFAIGENIAV
TPGKVIAQNDICQVLQYEASTETVLKRPLLICPPWINKFYVLDLNPEKSFIKWAVDQGHTVFVISWVNPDERHATKDWEA
YARDGIGFALDIIEQATEEREVNSIGYCVGGTLLAATLALHAAERDDRIRSATLFTTQVDFTHAGDLKVFVDDDQIRHLE
ANMSATGYLEGSKMASAFNMLRASELIWPYFVNNYLKGQDPLPFDLLYWNSDSTRMPAANHSFYLRNCYLENRLAKGEMV
LAGRRISLGDVKIPVYNLATKEDHIAPAKSVFLGSSSFGGKVTFVLSGSGHIAGVVNPPARGKYQYWTGGPPKGDVETWM
SKAKETAGSWWPHWQSWVERLDKRRVPARKAGGPLNSIEEAPGSYVRMRA

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 68333; Mature: 68201

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEKAEGAKGFAGFDPKSVEPYIVKDPENLAINMARAAEQLGKAASAWLAPRESGEKTD
CCCCCCCCCCCCCCCCCCCCCCEEEECCCHHEEEHHHHHHHHHHHHHHHCCCCCCCCCCH
SFAEPVSDMVKTLSKVSEYWLTDPRRTLEAQTHLLGSFFDMWSRTVHRMAGDAVDEPASL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
QRNDKRFSDEDWVRNPFFDFIRQAYFVTSDWAERMVNDAEGLDDHTRHKAAFYVRQIASA
CCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LSPTNFITTNPQLYRETVATSGANLVKGMQMLAEDIAAGRGELRLRQTDTSKFAIGENIA
CCCCCEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCEE
VTPGKVIAQNDICQVLQYEASTETVLKRPLLICPPWINKFYVLDLNPEKSFIKWAVDQGH
ECCCCEEEHHHHHHHHHHCCCHHHHHHCCCEEECCCCCEEEEEEECCCCCHHHHEECCCC
TVFVISWVNPDERHATKDWEAYARDGIGFALDIIEQATEEREVNSIGYCVGGTLLAATLA
EEEEEEECCCCHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
LHAAERDDRIRSATLFTTQVDFTHAGDLKVFVDDDQIRHLEANMSATGYLEGSKMASAFN
HHHHHHCCCCEEEEEEEEEECEEECCCEEEEECCCHHHHHHCCCCCCEEECCHHHHHHHH
MLRASELIWPYFVNNYLKGQDPLPFDLLYWNSDSTRMPAANHSFYLRNCYLENRLAKGEM
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHCCCCCE
VLAGRRISLGDVKIPVYNLATKEDHIAPAKSVFLGSSSFGGKVTFVLSGSGHIAGVVNPP
EEECCEEECCCEEEEEEECCCCCCCCCCHHHEEECCCCCCCEEEEEEECCCCEEEEECCC
ARGKYQYWTGGPPKGDVETWMSKAKETAGSWWPHWQSWVERLDKRRVPARKAGGPLNSIE
CCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHCCCCCCHHH
EAPGSYVRMRA
HCCCCEEEECC
>Mature Secondary Structure 
TAEKAEGAKGFAGFDPKSVEPYIVKDPENLAINMARAAEQLGKAASAWLAPRESGEKTD
CCCCCCCCCCCCCCCCCCCCCEEEECCCHHEEEHHHHHHHHHHHHHHHCCCCCCCCCCH
SFAEPVSDMVKTLSKVSEYWLTDPRRTLEAQTHLLGSFFDMWSRTVHRMAGDAVDEPASL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
QRNDKRFSDEDWVRNPFFDFIRQAYFVTSDWAERMVNDAEGLDDHTRHKAAFYVRQIASA
CCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LSPTNFITTNPQLYRETVATSGANLVKGMQMLAEDIAAGRGELRLRQTDTSKFAIGENIA
CCCCCEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCEE
VTPGKVIAQNDICQVLQYEASTETVLKRPLLICPPWINKFYVLDLNPEKSFIKWAVDQGH
ECCCCEEEHHHHHHHHHHCCCHHHHHHCCCEEECCCCCEEEEEEECCCCCHHHHEECCCC
TVFVISWVNPDERHATKDWEAYARDGIGFALDIIEQATEEREVNSIGYCVGGTLLAATLA
EEEEEEECCCCHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
LHAAERDDRIRSATLFTTQVDFTHAGDLKVFVDDDQIRHLEANMSATGYLEGSKMASAFN
HHHHHHCCCCEEEEEEEEEECEEECCCEEEEECCCHHHHHHCCCCCCEEECCHHHHHHHH
MLRASELIWPYFVNNYLKGQDPLPFDLLYWNSDSTRMPAANHSFYLRNCYLENRLAKGEM
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHCCCCCE
VLAGRRISLGDVKIPVYNLATKEDHIAPAKSVFLGSSSFGGKVTFVLSGSGHIAGVVNPP
EEECCEEECCCEEEEEEECCCCCCCCCCHHHEEECCCCCCCEEEEEEECCCCEEEEECCC
ARGKYQYWTGGPPKGDVETWMSKAKETAGSWWPHWQSWVERLDKRRVPARKAGGPLNSIE
CCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHCCCCCCHHH
EAPGSYVRMRA
HCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7582015; 9734305; 11481430 [H]