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The map label for this gene is glnB [H]

Identifier: 150396602

GI number: 150396602

Start: 1463770

End: 1464108

Strand: Direct

Name: glnB [H]

Synonym: Smed_1388

Alternate gene names: 150396602

Gene position: 1463770-1464108 (Clockwise)

Preceding gene: 150396600

Following gene: 150396603

Centisome position: 38.7

GC content: 56.93

Gene sequence:

>339_bases
ATGAAAAAGATCGAAGCGATCATTAAGCCTTTCAAGCTCGACGAAGTGAAGGAAGCCCTTCAGGAGGTCGGCTTGCAGGG
GATTACCGTCACGGAAGCCAAGGGCTTCGGGCGGCAGAAAGGTCATACGGAGCTTTACCGGGGTGCAGAATACGTCGTGG
ACTTCCTGCCGAAGGTGAAGGTCGAGGTTGTGCTGGCCGATGAGAATGCGGAGGCCGTGATCGAAGCCATCCGTAACGCC
GCACAAACCGGCCGGATCGGCGACGGCAAGATATTCGTATCCAACGTGGAAGAGGTCATCCGCATCCGCACCGGTGAAAC
CGGAATCGACGCCATCTGA

Upstream 100 bases:

>100_bases
TCTGCGCGTCATGCCCCTACAAAAGTGGCACGCTACGTGCATAGTGGGAAACCAAGCGGGCAGAACCTGCTTTAAGGGAG
AAGCGGAGAGACATTTTCTC

Downstream 100 bases:

>100_bases
TCACGTCGCCAAGCGACGTTACCACATCCGGGGCCATCCGGCCCCGTTTCCAATCCTCGTCATCACAGGGAATTGCAACA
AATGACGACTGCGAGTGAAG

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKKIEAIIKPFKLDEVKEALQEVGLQGITVTEAKGFGRQKGHTELYRGAEYVVDFLPKVKVEVVLADENAEAVIEAIRNA
AQTGRIGDGKIFVSNVEEVIRIRTGETGIDAI

Sequences:

>Translated_112_residues
MKKIEAIIKPFKLDEVKEALQEVGLQGITVTEAKGFGRQKGHTELYRGAEYVVDFLPKVKVEVVLADENAEAVIEAIRNA
AQTGRIGDGKIFVSNVEEVIRIRTGETGIDAI
>Mature_112_residues
MKKIEAIIKPFKLDEVKEALQEVGLQGITVTEAKGFGRQKGHTELYRGAEYVVDFLPKVKVEVVLADENAEAVIEAIRNA
AQTGRIGDGKIFVSNVEEVIRIRTGETGIDAI

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=106, Percent_Identity=71.6981132075472, Blast_Score=159, Evalue=3e-41,
Organism=Escherichia coli, GI1786655, Length=106, Percent_Identity=65.0943396226415, Blast_Score=149, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12281; Mature: 12281

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIEAIIKPFKLDEVKEALQEVGLQGITVTEAKGFGRQKGHTELYRGAEYVVDFLPKVK
CCCHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCE
VEVVLADENAEAVIEAIRNAAQTGRIGDGKIFVSNVEEVIRIRTGETGIDAI
EEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEHHCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKIEAIIKPFKLDEVKEALQEVGLQGITVTEAKGFGRQKGHTELYRGAEYVVDFLPKVK
CCCHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCE
VEVVLADENAEAVIEAIRNAAQTGRIGDGKIFVSNVEEVIRIRTGETGIDAI
EEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEHHCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8931324; 11481430 [H]