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The map label for this gene is tgt [H]

Identifier: 150396486

GI number: 150396486

Start: 1361678

End: 1362808

Strand: Direct

Name: tgt [H]

Synonym: Smed_1268

Alternate gene names: 150396486

Gene position: 1361678-1362808 (Clockwise)

Preceding gene: 150396485

Following gene: 150396492

Centisome position: 36.01

GC content: 62.51

Gene sequence:

>1131_bases
ATGACCGAAGCGTTTCAATTCAAACTGCTTTCAAGTGACGGCAATGCACGCCGCGGCGAGGTCTTGACGCCTCGCGGGAC
GATCCGCACGCCAGCCTTCATGCCGGTCGGCACCGTCGGCACCGTCAAGGCGATGTATCTCGACCAGGTCCGCGAGCTGG
GTGCGGATATCATTCTGGGCAATACTTATCACCTGATGCTGCGCCCGGGGGCCGAGCGCGTCGCCCGGCTCGGCGGTCTG
CACAAGTTCATCCGCTGGGATAGACCGATCCTGACCGACAGCGGCGGATTCCAGGTCATGTCGTTGTCGAGCTTGCGCAA
GCTCAACGAACAGGGCGTGACCTTCAAGAGCCATGTCGACGGCGCGCTCTATCATATGTCGCCGGAACGCTCGATCGAAA
TACAAGGCTTGCTCGGCAGCGATATTCAGATGCAGCTCGACGAATGCGTCGCGCTGCCGGCAGAACCCGGCGAAATCGAG
CGCGCCATGGAGATGTCGCTGCGCTGGGCCGAGCGCTGCAAGGTCGCCTTCGGCGACCAGCCGGGCAAGGCGATGTTTGG
CATCGTTCAGGGCGGCGATATCCCCAAGCTGCGTGAACGCTCGGCGTCGGCGCTCAACGAGCTCGACCTCAAGGGCTATG
CGGTAGGCGGCCTCGCCGTCGGTGAGCCGCAAGAGGTGATGCTCGATATGCTCGACGTGACTTGCCCTGTCCTGCCGGCC
GACAAACCACGCTACCTTATGGGCGTCGGCACCCCGGACGATATCCTGAAGTCGGTCGCCCATGGTGTGGATATGTTCGA
CTGCGTCATGCCGACCCGCTCCGGCCGTCACGGCCTCGCCTTCACTCGCTACGGCCGTATCAACCTGCGTAATGCCCGAC
ACGCGGAGGATGCGCGCCCACTCGACGAACAGTCCTCCTGTCCGGCGGCGCGCGACTATTCGCGCGCTTACCTGCACCAC
CTGATTCGTTCAAACGAATCGCTTGGCGGGATGCTGCTAAGCTGGAACAATCTGGCCTATTATCAGGAGCTGATGGCCGG
TATCCGCAAGGCGATCGAGGAAGGGCGCTATTCCGATTTCATGGCGGAGACCATCGAAGGCTGGCAGCGCGGCGATCTCG
CCCCCGTGTAA

Upstream 100 bases:

>100_bases
GGGGCTCGAAACAATGCGCGACGCTTATGCCCATGCGATCGCAACGGGGTACCGTTTCTATTCCTATGGCGATTCGAGCC
TGCTCTTCCGGAAAGACTAA

Downstream 100 bases:

>100_bases
TCATGTAAAATCAAGGTCGTATGGAGATTACTTAATCTTCATACATAGCCCGTCGCAACGCGCGCCTGAAAGGCAACTGC
TTTACGCCCGGGGTTCTGGA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTEAFQFKLLSSDGNARRGEVLTPRGTIRTPAFMPVGTVGTVKAMYLDQVRELGADIILGNTYHLMLRPGAERVARLGGL
HKFIRWDRPILTDSGGFQVMSLSSLRKLNEQGVTFKSHVDGALYHMSPERSIEIQGLLGSDIQMQLDECVALPAEPGEIE
RAMEMSLRWAERCKVAFGDQPGKAMFGIVQGGDIPKLRERSASALNELDLKGYAVGGLAVGEPQEVMLDMLDVTCPVLPA
DKPRYLMGVGTPDDILKSVAHGVDMFDCVMPTRSGRHGLAFTRYGRINLRNARHAEDARPLDEQSSCPAARDYSRAYLHH
LIRSNESLGGMLLSWNNLAYYQELMAGIRKAIEEGRYSDFMAETIEGWQRGDLAPV

Sequences:

>Translated_376_residues
MTEAFQFKLLSSDGNARRGEVLTPRGTIRTPAFMPVGTVGTVKAMYLDQVRELGADIILGNTYHLMLRPGAERVARLGGL
HKFIRWDRPILTDSGGFQVMSLSSLRKLNEQGVTFKSHVDGALYHMSPERSIEIQGLLGSDIQMQLDECVALPAEPGEIE
RAMEMSLRWAERCKVAFGDQPGKAMFGIVQGGDIPKLRERSASALNELDLKGYAVGGLAVGEPQEVMLDMLDVTCPVLPA
DKPRYLMGVGTPDDILKSVAHGVDMFDCVMPTRSGRHGLAFTRYGRINLRNARHAEDARPLDEQSSCPAARDYSRAYLHH
LIRSNESLGGMLLSWNNLAYYQELMAGIRKAIEEGRYSDFMAETIEGWQRGDLAPV
>Mature_375_residues
TEAFQFKLLSSDGNARRGEVLTPRGTIRTPAFMPVGTVGTVKAMYLDQVRELGADIILGNTYHLMLRPGAERVARLGGLH
KFIRWDRPILTDSGGFQVMSLSSLRKLNEQGVTFKSHVDGALYHMSPERSIEIQGLLGSDIQMQLDECVALPAEPGEIER
AMEMSLRWAERCKVAFGDQPGKAMFGIVQGGDIPKLRERSASALNELDLKGYAVGGLAVGEPQEVMLDMLDVTCPVLPAD
KPRYLMGVGTPDDILKSVAHGVDMFDCVMPTRSGRHGLAFTRYGRINLRNARHAEDARPLDEQSSCPAARDYSRAYLHHL
IRSNESLGGMLLSWNNLAYYQELMAGIRKAIEEGRYSDFMAETIEGWQRGDLAPV

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=350, Percent_Identity=44, Blast_Score=306, Evalue=2e-83,
Organism=Escherichia coli, GI1786607, Length=359, Percent_Identity=53.2033426183844, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=41.4364640883978, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI25146502, Length=178, Percent_Identity=27.5280898876405, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920510, Length=376, Percent_Identity=46.2765957446808, Blast_Score=327, Evalue=6e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41599; Mature: 41468

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAFQFKLLSSDGNARRGEVLTPRGTIRTPAFMPVGTVGTVKAMYLDQVRELGADIILG
CCCCEEEEEECCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEC
NTYHLMLRPGAERVARLGGLHKFIRWDRPILTDSGGFQVMSLSSLRKLNEQGVTFKSHVD
CEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHCCCEEHHCCC
GALYHMSPERSIEIQGLLGSDIQMQLDECVALPAEPGEIERAMEMSLRWAERCKVAFGDQ
CEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
PGKAMFGIVQGGDIPKLRERSASALNELDLKGYAVGGLAVGEPQEVMLDMLDVTCPVLPA
CCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCEECCCHHHHHHHHHHHHCCCCCC
DKPRYLMGVGTPDDILKSVAHGVDMFDCVMPTRSGRHGLAFTRYGRINLRNARHAEDARP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEECCEEECCCCCCCCCCC
LDEQSSCPAARDYSRAYLHHLIRSNESLGGMLLSWNNLAYYQELMAGIRKAIEEGRYSDF
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHH
MAETIEGWQRGDLAPV
HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TEAFQFKLLSSDGNARRGEVLTPRGTIRTPAFMPVGTVGTVKAMYLDQVRELGADIILG
CCCEEEEEECCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEC
NTYHLMLRPGAERVARLGGLHKFIRWDRPILTDSGGFQVMSLSSLRKLNEQGVTFKSHVD
CEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHCCCEEHHCCC
GALYHMSPERSIEIQGLLGSDIQMQLDECVALPAEPGEIERAMEMSLRWAERCKVAFGDQ
CEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
PGKAMFGIVQGGDIPKLRERSASALNELDLKGYAVGGLAVGEPQEVMLDMLDVTCPVLPA
CCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCEECCCHHHHHHHHHHHHCCCCCC
DKPRYLMGVGTPDDILKSVAHGVDMFDCVMPTRSGRHGLAFTRYGRINLRNARHAEDARP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEECCEEECCCCCCCCCCC
LDEQSSCPAARDYSRAYLHHLIRSNESLGGMLLSWNNLAYYQELMAGIRKAIEEGRYSDF
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHH
MAETIEGWQRGDLAPV
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]