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The map label for this gene is cls [H]

Identifier: 150396374

GI number: 150396374

Start: 1229573

End: 1231036

Strand: Direct

Name: cls [H]

Synonym: Smed_1155

Alternate gene names: 150396374

Gene position: 1229573-1231036 (Clockwise)

Preceding gene: 150396372

Following gene: 150396376

Centisome position: 32.51

GC content: 60.45

Gene sequence:

>1464_bases
ATGATCGCTTTCGTAGAGAGTTATTGGCCGCATTTTCTGGCACTGCTGTCCATCGCCCTGGGCGTTCCGGCGATCATCCA
TGCCGCTATGACGAAGGATGACGTGCGTGCGGCCGCCGGCTGGGTCGGGGTCGTCCTCCTGTCACCGGTCATCGGTGCAG
TCATCTACGCGGTCGCCGGTATCAACCGGATGCGCCGCTCGTCCATCGGCCTGCAGCGTTCCTTGCTGCGCTCGACGGAG
CGGGACCCGTTCGGGCGCTTCGATATCACGCATGATCAGGTGGTGGCGCGCTTCGGGCAGCGTTTTGCGGCAATGAAGAT
GCTTGGGGACCGGGTCGCACGCTTTACGATGTCAGCCGGAAACCGCATCACCATGCTCGAGGGCGGCGACGCGGTCTATG
CGGCGATGCTCGACGCGATTTCGTCCGCCCGCCGAAGTATTCTCATCGAGAGCTATATTTTCGACCGTGATGCCATCGGC
ATGCGTTTTGCCGACGCCCTGATCGCCGCAGTCAAACGCGGTGTCAGCGTGCGCGTCCTGATTGACGCCGTCGGCGCTCG
CTACTCCGTGCCGAGTATCGTGGGCTACCTCAAGGAAGGGGGCGTGCCGACCGCCGTCTTCAACGGCAATATCATCATGG
GTCTGAGGCTGCCCTATGCAAATCTCAGGACCCACCGCAAGATCGTGGTCATCGATGGCGCGGTCGCCTTCGCCGGGGGC
ATGAACATTCGCGCAGGTTTTGCCGCTGAAATCGTAGGCAGCGCGGCTTCTTTCGACACGCATTTCCAAGTAACCGGGCC
GGTCGTCTCCGACATCTTTCAGGTTGCCGCGGAAGACTGGCAGTTTTCGAGCGAAGAGGTGCTGGCGGGTGACGCCTGGC
GCCTTGCCGACCTCCCGGTTGAGCCGGATGTGGGGTCGGTGCTGATGCGAGCAGTTCCCTCGGGGCCTGACAACACCAAT
GAGACCAATCACAAGATGCTTATGGGCGCTTTTTCGATTGCGCGAAAGCATATCCGGTTGATGTCGCCATATTTCCTGCC
GGACAAGGAATTGGTCAGCGCGTTGGTGACTGCTGCAAGGAAAGGCGTCGAGGTCGACATCATCGTACCCTCCGTCAACA
ATCTGACACTCGTCGACCGAGCGATGACGGCACAGTTCGACCAGGTGCTGAAAGGCCATTGCCGAGTGTGGAGGGCGAAA
GGTGCCTTCAACCATTCCAAGCTGCTGGTCATCGACGATCGCTGGGCCTATGTCGGTTCGACCAATCTGGATCCCCGTTC
CCTGCGCCTCAATTTCGAGTTCGATCTCGAAATACTCGACGAAGCCTTCGCCCGTGCGATCGGTGAAAAGGTCTGTGCAA
TACGAGCGACCGCGGACGAGGTGACGCTTGCAGGTCTGCGCGCGCAGCCGCTCGTCAACCGCCTGGCGAATCGCTTGCTC
TGGCTCGGATCGCCATATCTTTAG

Upstream 100 bases:

>100_bases
GTCTGCGACATCGTCGGGCGCCAGGCCGACTGCTTTTAAGGCCTGTATACCCTTGAACACCCGAATTGATTCCGTTCGGC
GTCCGTCTATAGTCCCGCGC

Downstream 100 bases:

>100_bases
CGGTGGCGCGCAGTGTCAGACCGAAAAACTTGTCCCACACCCGCAGCTTGCGACGGCGTTCGGGTTTTTGATCTGGAAGG
ACTGTCCAAGCAGGTTGTCG

Product: phospholipase D/transphosphatidylase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MIAFVESYWPHFLALLSIALGVPAIIHAAMTKDDVRAAAGWVGVVLLSPVIGAVIYAVAGINRMRRSSIGLQRSLLRSTE
RDPFGRFDITHDQVVARFGQRFAAMKMLGDRVARFTMSAGNRITMLEGGDAVYAAMLDAISSARRSILIESYIFDRDAIG
MRFADALIAAVKRGVSVRVLIDAVGARYSVPSIVGYLKEGGVPTAVFNGNIIMGLRLPYANLRTHRKIVVIDGAVAFAGG
MNIRAGFAAEIVGSAASFDTHFQVTGPVVSDIFQVAAEDWQFSSEEVLAGDAWRLADLPVEPDVGSVLMRAVPSGPDNTN
ETNHKMLMGAFSIARKHIRLMSPYFLPDKELVSALVTAARKGVEVDIIVPSVNNLTLVDRAMTAQFDQVLKGHCRVWRAK
GAFNHSKLLVIDDRWAYVGSTNLDPRSLRLNFEFDLEILDEAFARAIGEKVCAIRATADEVTLAGLRAQPLVNRLANRLL
WLGSPYL

Sequences:

>Translated_487_residues
MIAFVESYWPHFLALLSIALGVPAIIHAAMTKDDVRAAAGWVGVVLLSPVIGAVIYAVAGINRMRRSSIGLQRSLLRSTE
RDPFGRFDITHDQVVARFGQRFAAMKMLGDRVARFTMSAGNRITMLEGGDAVYAAMLDAISSARRSILIESYIFDRDAIG
MRFADALIAAVKRGVSVRVLIDAVGARYSVPSIVGYLKEGGVPTAVFNGNIIMGLRLPYANLRTHRKIVVIDGAVAFAGG
MNIRAGFAAEIVGSAASFDTHFQVTGPVVSDIFQVAAEDWQFSSEEVLAGDAWRLADLPVEPDVGSVLMRAVPSGPDNTN
ETNHKMLMGAFSIARKHIRLMSPYFLPDKELVSALVTAARKGVEVDIIVPSVNNLTLVDRAMTAQFDQVLKGHCRVWRAK
GAFNHSKLLVIDDRWAYVGSTNLDPRSLRLNFEFDLEILDEAFARAIGEKVCAIRATADEVTLAGLRAQPLVNRLANRLL
WLGSPYL
>Mature_487_residues
MIAFVESYWPHFLALLSIALGVPAIIHAAMTKDDVRAAAGWVGVVLLSPVIGAVIYAVAGINRMRRSSIGLQRSLLRSTE
RDPFGRFDITHDQVVARFGQRFAAMKMLGDRVARFTMSAGNRITMLEGGDAVYAAMLDAISSARRSILIESYIFDRDAIG
MRFADALIAAVKRGVSVRVLIDAVGARYSVPSIVGYLKEGGVPTAVFNGNIIMGLRLPYANLRTHRKIVVIDGAVAFAGG
MNIRAGFAAEIVGSAASFDTHFQVTGPVVSDIFQVAAEDWQFSSEEVLAGDAWRLADLPVEPDVGSVLMRAVPSGPDNTN
ETNHKMLMGAFSIARKHIRLMSPYFLPDKELVSALVTAARKGVEVDIIVPSVNNLTLVDRAMTAQFDQVLKGHCRVWRAK
GAFNHSKLLVIDDRWAYVGSTNLDPRSLRLNFEFDLEILDEAFARAIGEKVCAIRATADEVTLAGLRAQPLVNRLANRLL
WLGSPYL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=489, Percent_Identity=26.3803680981595, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1787007, Length=349, Percent_Identity=31.8051575931232, Blast_Score=148, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 53084; Mature: 53084

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAFVESYWPHFLALLSIALGVPAIIHAAMTKDDVRAAAGWVGVVLLSPVIGAVIYAVAG
CEEEHHHHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INRMRRSSIGLQRSLLRSTERDPFGRFDITHDQVVARFGQRFAAMKMLGDRVARFTMSAG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NRITMLEGGDAVYAAMLDAISSARRSILIESYIFDRDAIGMRFADALIAAVKRGVSVRVL
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEE
IDAVGARYSVPSIVGYLKEGGVPTAVFNGNIIMGLRLPYANLRTHRKIVVIDGAVAFAGG
EEHHCCCCCHHHHHHHHHCCCCCEEEECCCEEEEEECCCHHHCCCCEEEEEECCEEEECC
MNIRAGFAAEIVGSAASFDTHFQVTGPVVSDIFQVAAEDWQFSSEEVLAGDAWRLADLPV
CCCCCCHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCEECCCCEEEECCCC
EPDVGSVLMRAVPSGPDNTNETNHKMLMGAFSIARKHIRLMSPYFLPDKELVSALVTAAR
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KGVEVDIIVPSVNNLTLVDRAMTAQFDQVLKGHCRVWRAKGAFNHSKLLVIDDRWAYVGS
CCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECCEEEECC
TNLDPRSLRLNFEFDLEILDEAFARAIGEKVCAIRATADEVTLAGLRAQPLVNRLANRLL
CCCCCCEEEEEEEECHHHHHHHHHHHHHHHHEEEEECCCCEEECCCCHHHHHHHHHHHHH
WLGSPYL
HCCCCCC
>Mature Secondary Structure
MIAFVESYWPHFLALLSIALGVPAIIHAAMTKDDVRAAAGWVGVVLLSPVIGAVIYAVAG
CEEEHHHHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INRMRRSSIGLQRSLLRSTERDPFGRFDITHDQVVARFGQRFAAMKMLGDRVARFTMSAG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NRITMLEGGDAVYAAMLDAISSARRSILIESYIFDRDAIGMRFADALIAAVKRGVSVRVL
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEE
IDAVGARYSVPSIVGYLKEGGVPTAVFNGNIIMGLRLPYANLRTHRKIVVIDGAVAFAGG
EEHHCCCCCHHHHHHHHHCCCCCEEEECCCEEEEEECCCHHHCCCCEEEEEECCEEEECC
MNIRAGFAAEIVGSAASFDTHFQVTGPVVSDIFQVAAEDWQFSSEEVLAGDAWRLADLPV
CCCCCCHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCEECCCCEEEECCCC
EPDVGSVLMRAVPSGPDNTNETNHKMLMGAFSIARKHIRLMSPYFLPDKELVSALVTAAR
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KGVEVDIIVPSVNNLTLVDRAMTAQFDQVLKGHCRVWRAKGAFNHSKLLVIDDRWAYVGS
CCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECCEEEECC
TNLDPRSLRLNFEFDLEILDEAFARAIGEKVCAIRATADEVTLAGLRAQPLVNRLANRLL
CCCCCCEEEEEEEECHHHHHHHHHHHHHHHHEEEEECCCCEEECCCCHHHHHHHHHHHHH
WLGSPYL
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9443601 [H]