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The map label for this gene is lipA

Identifier: 150396303

GI number: 150396303

Start: 1151591

End: 1152562

Strand: Direct

Name: lipA

Synonym: Smed_1083

Alternate gene names: 150396303

Gene position: 1151591-1152562 (Clockwise)

Preceding gene: 150396302

Following gene: 150396304

Centisome position: 30.45

GC content: 62.35

Gene sequence:

>972_bases
ATGGTAACGGTTTTCGACGCCGTCGCGGATCGGGCGCAGCGTGTCCGCCACCCGGAAAAGGCCCACCGGCCCGATACCGA
AGTCCTGCGCAAGCCGGACTGGATCCGGGTGAAGGCTCCAACCTCTAAAGGGTATCAGGAGACCCGTTCGATCGTGAAGA
GCCATAACCTCGTCACGGTCTGCGAGGAGGCCGGCTGCCCGAACATTGGCGAGTGCTGGGACAAGAAGCACGCGACCTTC
ATGATCATGGGCGAGATCTGCACGCGCGCCTGTGCCTTCTGCAACGTCGCGACCGGCAGGCCCAATGCCCTCGATCTGGA
CGAGCCCGTGAATGTCGCCAAGGCCGTCAAGCAGATGGGTCTTTCCCATGTCGTCATCACCTCCGTCGATCGAGACGATC
TGGAGGATGGCGGCGCAGAGCATTTCGAGAGGGTCATCTTCGCAATCCGGGAAGCTTCGCCGCAGACCACGATCGAAATA
CTGACGCCGGATTTCCTGCGCAAGCCGGGCGCACTGGAGCGCGTCGTGGCCGCCAAGCCGGATGTATTCAATCACAACCT
GGAGACGGTGCCCTCCAACTACCTGACCGTCAGGCCGGGCGCGCGGTATTTCCACTCAATCCGGCTGCTGCAGCGCGTCA
AGGAGCTCGACCCGACCATGTTCACCAAGTCTGGCATCATGGTCGGCCTGGGCGAGGAACGCAACGAAGTGCTGCAGTTG
ATGGACGACCTGCGCACGGCGGACGTCGACTTCCTGACGATCGGCCAGTACCTGCAGCCGACCCGCAAGCACCACAAGGT
CGAGAAATTCGTGACACCGGATGAGTTCAAGTCCTACGAAACGGTGGCCTACACCAAAGGCTTCCTGATGGTCTCCTCGA
GCCCGCTGACCCGCTCGTCGCATCACGCCGGCGACGACTTCGCGCGGCTGAAGGCGGCACGGGAGAAGAAGCTGCTTGCT
GCGGCGGAGTAG

Upstream 100 bases:

>100_bases
GCCATGCAAATGGTGGCCTGACTTTTCATGTACGGCTGTTTATATAAGACGTTAGGTGTGGCTGGCCTGCGGCCTTGCCC
GTCACGATGAAGGAATTCAC

Downstream 100 bases:

>100_bases
CTGGTTCCGCCCTCATGCGCCTGGCGGCACCTGCTCCCTGCAAGAGCGGGGCGTGGGGATTTGCAGCAACGCTCCACACT
CCTTTGTTCGACAAGGTTCG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MVTVFDAVADRAQRVRHPEKAHRPDTEVLRKPDWIRVKAPTSKGYQETRSIVKSHNLVTVCEEAGCPNIGECWDKKHATF
MIMGEICTRACAFCNVATGRPNALDLDEPVNVAKAVKQMGLSHVVITSVDRDDLEDGGAEHFERVIFAIREASPQTTIEI
LTPDFLRKPGALERVVAAKPDVFNHNLETVPSNYLTVRPGARYFHSIRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
MDDLRTADVDFLTIGQYLQPTRKHHKVEKFVTPDEFKSYETVAYTKGFLMVSSSPLTRSSHHAGDDFARLKAAREKKLLA
AAE

Sequences:

>Translated_323_residues
MVTVFDAVADRAQRVRHPEKAHRPDTEVLRKPDWIRVKAPTSKGYQETRSIVKSHNLVTVCEEAGCPNIGECWDKKHATF
MIMGEICTRACAFCNVATGRPNALDLDEPVNVAKAVKQMGLSHVVITSVDRDDLEDGGAEHFERVIFAIREASPQTTIEI
LTPDFLRKPGALERVVAAKPDVFNHNLETVPSNYLTVRPGARYFHSIRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
MDDLRTADVDFLTIGQYLQPTRKHHKVEKFVTPDEFKSYETVAYTKGFLMVSSSPLTRSSHHAGDDFARLKAAREKKLLA
AAE
>Mature_323_residues
MVTVFDAVADRAQRVRHPEKAHRPDTEVLRKPDWIRVKAPTSKGYQETRSIVKSHNLVTVCEEAGCPNIGECWDKKHATF
MIMGEICTRACAFCNVATGRPNALDLDEPVNVAKAVKQMGLSHVVITSVDRDDLEDGGAEHFERVIFAIREASPQTTIEI
LTPDFLRKPGALERVVAAKPDVFNHNLETVPSNYLTVRPGARYFHSIRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
MDDLRTADVDFLTIGQYLQPTRKHHKVEKFVTPDEFKSYETVAYTKGFLMVSSSPLTRSSHHAGDDFARLKAAREKKLLA
AAE

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=316, Percent_Identity=45.253164556962, Blast_Score=285, Evalue=3e-77,
Organism=Homo sapiens, GI37577164, Length=267, Percent_Identity=46.0674157303371, Blast_Score=244, Evalue=6e-65,
Organism=Escherichia coli, GI1786846, Length=278, Percent_Identity=52.8776978417266, Blast_Score=300, Evalue=9e-83,
Organism=Caenorhabditis elegans, GI32564533, Length=291, Percent_Identity=41.2371134020619, Blast_Score=234, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=311, Percent_Identity=42.1221864951768, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=44.0559440559441, Blast_Score=251, Evalue=5e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_SINMW (A6U8F5)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001326770.1
- ProteinModelPortal:   A6U8F5
- STRING:   A6U8F5
- GeneID:   5321929
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_1083
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   RRESPET
- ProtClustDB:   PRK05481
- BioCyc:   SMED366394:SMED_1083-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36283; Mature: 36283

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVFDAVADRAQRVRHPEKAHRPDTEVLRKPDWIRVKAPTSKGYQETRSIVKSHNLVTV
CEEHHHHHHHHHHHHCCCHHHCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCEEEE
CEEAGCPNIGECWDKKHATFMIMGEICTRACAFCNVATGRPNALDLDEPVNVAKAVKQMG
EHHCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
LSHVVITSVDRDDLEDGGAEHFERVIFAIREASPQTTIEILTPDFLRKPGALERVVAAKP
CCEEEEEECCHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCHHHHHHHCCC
DVFNHNLETVPSNYLTVRPGARYFHSIRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
CHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCHHEECCCEEEECCCCHHHHHHH
MDDLRTADVDFLTIGQYLQPTRKHHKVEKFVTPDEFKSYETVAYTKGFLMVSSSPLTRSS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHEEECCEEEEECCCCCCCC
HHAGDDFARLKAAREKKLLAAAE
CCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVTVFDAVADRAQRVRHPEKAHRPDTEVLRKPDWIRVKAPTSKGYQETRSIVKSHNLVTV
CEEHHHHHHHHHHHHCCCHHHCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCEEEE
CEEAGCPNIGECWDKKHATFMIMGEICTRACAFCNVATGRPNALDLDEPVNVAKAVKQMG
EHHCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
LSHVVITSVDRDDLEDGGAEHFERVIFAIREASPQTTIEILTPDFLRKPGALERVVAAKP
CCEEEEEECCHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCHHHHHHHCCC
DVFNHNLETVPSNYLTVRPGARYFHSIRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
CHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCHHEECCCEEEECCCCHHHHHHH
MDDLRTADVDFLTIGQYLQPTRKHHKVEKFVTPDEFKSYETVAYTKGFLMVSSSPLTRSS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHEEECCEEEEECCCCCCCC
HHAGDDFARLKAAREKKLLAAAE
CCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA