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The map label for this gene is pdhC [H]

Identifier: 150396298

GI number: 150396298

Start: 1146958

End: 1148331

Strand: Direct

Name: pdhC [H]

Synonym: Smed_1078

Alternate gene names: 150396298

Gene position: 1146958-1148331 (Clockwise)

Preceding gene: 150396297

Following gene: 150396299

Centisome position: 30.33

GC content: 63.83

Gene sequence:

>1374_bases
ATGCCAATCAACATCACCATGCCGGCCCTCTCTCCGACGATGGAAGAAGGCAATCTCGCCAAGTGGCTGGTCAAGGAAGG
CGATAAGGTCAAATCCGGCGACGTGATCGCCGAGATCGAGACCGACAAGGCGACGATGGAAGTGGAAGCCGTCGATGAGG
GCACGGTCGCCAAAATCGTCGTTCCCGCCGGAACCGAAGGCGTCAAGGTCAATGCGTTGATCGCGGTTCTCGCCGCTGAT
GGCGAGGACGTTGCCACCGCTGCCAAGGGTGGCAACGGGGCGGCGGGAGAAACCGCCGCAACGAAGCCGCAGGAAACGGC
GGAAGCAGCACCCGCCGCGGCAAAGGCGCCCGCGGAAGCAAAGGCACCCGCCGAAGCGAACGCCCCGCAGGCGGACGCAC
CGGCACCGCAAGCAGCCTCGCCCACACCGGCCGCAGGCGACGGGAAGCGGATCTTTTCTTCGCCGCTGGCGCGCCGCCTC
GCCAGGGAGGCGGGTATCGACCTGTCGGCAATCGCCGGGTCCGGGCCGCATGGCCGCGTCATCAAGAAGGATGTCGAGGC
CGCCGCTTCCGGCGGTGCCGCCAAGACCGCCGCTGCTCCGGCAGCAGCTCCGGCCCCGTCGGCGCCTGCCAAGGGCATGT
CGGAAGATGCGGTGTTGAAGCTCTTCGAGCCAGGCTCCTACGAGCTCGTGCCGCATGACGGCATGCGCAAGACGATCGCC
AAGCGCCTCGTCGAATCGAAGCAGACGATCCCGCATTTCTACGTCTCGGTCGATTGTGAACTCGACGCGTTGCTGGCACT
GCGCGCCCAGCTGAATTCCGCAGCACCTGAAAAAGACGGTAAGTCGGTCTACAAGCTTTCCGTCAACGACATGGTGATCA
AGGCGTTGGCCCTGGCGCTGCGTGACGTCCCGGATGCGAATGTCTCCTGGACGGATCAGAACATGGTCAAGCACAAGCAC
GCGGATGTCGGCGTTGCCGTCTCCATTCCCGGCGGCCTGATCACCCCCATCATCCGCCAGGCGGAATTGAAAAGCCTCTC
GGCGATTTCCAACGAGATGAAGGACCTCGGCAAGCGGGCGAAAGAGCGCAAGCTCAAGCCGGAAGAATATCAGGGCGGCA
CCACGGCCGTCTCCAATATGGGCATGATGGGCGTCAAGAACTTCGCAGCAGTCGTCAATCCGCCGCACGCGACGATCCTC
GCGGTCGGTGCCGGCGAGGAACGCGTCGTCGTCAAGAACAAGGAGACGGTCATCGCCAATGTGATGACCGTGACGCTTTC
GACGGATCATCGCTGCGTGGACGGGGCATTGGGCGCCGAACTGCTCGCCGCCTTCAAGCGCTACATCGAAAGCCCGATGG
GCATGCTCGTCTGA

Upstream 100 bases:

>100_bases
TGCCCTATGCGGCCAATCTTGAAAAGCTCGCATTGCCGAGCGTCGCCGAAGTCGTCGAGGCGGTGAAAGCCGTCTGCTAC
AAATAAAAGGGGGCCGTTCG

Downstream 100 bases:

>100_bases
TGCGATCCGGACCGGCCACGCGCCGGTCCGCCCTTTCTCCGACGGTACGGGTGGGCAGATGAAGACAGTCCTTTGCTACG
GCGACAGTCTGACCTGGGGT

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MPINITMPALSPTMEEGNLAKWLVKEGDKVKSGDVIAEIETDKATMEVEAVDEGTVAKIVVPAGTEGVKVNALIAVLAAD
GEDVATAAKGGNGAAGETAATKPQETAEAAPAAAKAPAEAKAPAEANAPQADAPAPQAASPTPAAGDGKRIFSSPLARRL
AREAGIDLSAIAGSGPHGRVIKKDVEAAASGGAAKTAAAPAAAPAPSAPAKGMSEDAVLKLFEPGSYELVPHDGMRKTIA
KRLVESKQTIPHFYVSVDCELDALLALRAQLNSAAPEKDGKSVYKLSVNDMVIKALALALRDVPDANVSWTDQNMVKHKH
ADVGVAVSIPGGLITPIIRQAELKSLSAISNEMKDLGKRAKERKLKPEEYQGGTTAVSNMGMMGVKNFAAVVNPPHATIL
AVGAGEERVVVKNKETVIANVMTVTLSTDHRCVDGALGAELLAAFKRYIESPMGMLV

Sequences:

>Translated_457_residues
MPINITMPALSPTMEEGNLAKWLVKEGDKVKSGDVIAEIETDKATMEVEAVDEGTVAKIVVPAGTEGVKVNALIAVLAAD
GEDVATAAKGGNGAAGETAATKPQETAEAAPAAAKAPAEAKAPAEANAPQADAPAPQAASPTPAAGDGKRIFSSPLARRL
AREAGIDLSAIAGSGPHGRVIKKDVEAAASGGAAKTAAAPAAAPAPSAPAKGMSEDAVLKLFEPGSYELVPHDGMRKTIA
KRLVESKQTIPHFYVSVDCELDALLALRAQLNSAAPEKDGKSVYKLSVNDMVIKALALALRDVPDANVSWTDQNMVKHKH
ADVGVAVSIPGGLITPIIRQAELKSLSAISNEMKDLGKRAKERKLKPEEYQGGTTAVSNMGMMGVKNFAAVVNPPHATIL
AVGAGEERVVVKNKETVIANVMTVTLSTDHRCVDGALGAELLAAFKRYIESPMGMLV
>Mature_456_residues
PINITMPALSPTMEEGNLAKWLVKEGDKVKSGDVIAEIETDKATMEVEAVDEGTVAKIVVPAGTEGVKVNALIAVLAADG
EDVATAAKGGNGAAGETAATKPQETAEAAPAAAKAPAEAKAPAEANAPQADAPAPQAASPTPAAGDGKRIFSSPLARRLA
REAGIDLSAIAGSGPHGRVIKKDVEAAASGGAAKTAAAPAAAPAPSAPAKGMSEDAVLKLFEPGSYELVPHDGMRKTIAK
RLVESKQTIPHFYVSVDCELDALLALRAQLNSAAPEKDGKSVYKLSVNDMVIKALALALRDVPDANVSWTDQNMVKHKHA
DVGVAVSIPGGLITPIIRQAELKSLSAISNEMKDLGKRAKERKLKPEEYQGGTTAVSNMGMMGVKNFAAVVNPPHATILA
VGAGEERVVVKNKETVIANVMTVTLSTDHRCVDGALGAELLAAFKRYIESPMGMLV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=463, Percent_Identity=40.6047516198704, Blast_Score=344, Evalue=9e-95,
Organism=Homo sapiens, GI203098816, Length=484, Percent_Identity=35.1239669421488, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI203098753, Length=484, Percent_Identity=35.1239669421488, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI110671329, Length=453, Percent_Identity=25.8278145695364, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=42.5, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI19923748, Length=266, Percent_Identity=28.1954887218045, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786946, Length=455, Percent_Identity=29.8901098901099, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1786305, Length=430, Percent_Identity=29.7674418604651, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17560088, Length=460, Percent_Identity=41.9565217391304, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=36.3934426229508, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=452, Percent_Identity=30.7522123893805, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=458, Percent_Identity=28.6026200873362, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=461, Percent_Identity=43.3839479392625, Blast_Score=320, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6320352, Length=454, Percent_Identity=27.0925110132159, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321632, Length=192, Percent_Identity=36.4583333333333, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20129315, Length=460, Percent_Identity=42.8260869565217, Blast_Score=287, Evalue=9e-78,
Organism=Drosophila melanogaster, GI24582497, Length=449, Percent_Identity=42.3162583518931, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI18859875, Length=460, Percent_Identity=27.6086956521739, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=29.0748898678414, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 47093; Mature: 46962

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPINITMPALSPTMEEGNLAKWLVKEGDKVKSGDVIAEIETDKATMEVEAVDEGTVAKIV
CCEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VPAGTEGVKVNALIAVLAADGEDVATAAKGGNGAAGETAATKPQETAEAAPAAAKAPAEA
EECCCCCEEEEEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCHHCCCCCC
KAPAEANAPQADAPAPQAASPTPAAGDGKRIFSSPLARRLAREAGIDLSAIAGSGPHGRV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCH
IKKDVEAAASGGAAKTAAAPAAAPAPSAPAKGMSEDAVLKLFEPGSYELVPHDGMRKTIA
HHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCHHHHHH
KRLVESKQTIPHFYVSVDCELDALLALRAQLNSAAPEKDGKSVYKLSVNDMVIKALALAL
HHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHH
RDVPDANVSWTDQNMVKHKHADVGVAVSIPGGLITPIIRQAELKSLSAISNEMKDLGKRA
HCCCCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KERKLKPEEYQGGTTAVSNMGMMGVKNFAAVVNPPHATILAVGAGEERVVVKNKETVIAN
HHHCCCCHHHCCCCHHHHHCCCHHHHHHHHEECCCCEEEEEECCCCCEEEEECCCEEEEE
VMTVTLSTDHRCVDGALGAELLAAFKRYIESPMGMLV
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHCC
>Mature Secondary Structure 
PINITMPALSPTMEEGNLAKWLVKEGDKVKSGDVIAEIETDKATMEVEAVDEGTVAKIV
CEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VPAGTEGVKVNALIAVLAADGEDVATAAKGGNGAAGETAATKPQETAEAAPAAAKAPAEA
EECCCCCEEEEEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCHHCCCCCC
KAPAEANAPQADAPAPQAASPTPAAGDGKRIFSSPLARRLAREAGIDLSAIAGSGPHGRV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCH
IKKDVEAAASGGAAKTAAAPAAAPAPSAPAKGMSEDAVLKLFEPGSYELVPHDGMRKTIA
HHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCHHHHHH
KRLVESKQTIPHFYVSVDCELDALLALRAQLNSAAPEKDGKSVYKLSVNDMVIKALALAL
HHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHH
RDVPDANVSWTDQNMVKHKHADVGVAVSIPGGLITPIIRQAELKSLSAISNEMKDLGKRA
HCCCCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KERKLKPEEYQGGTTAVSNMGMMGVKNFAAVVNPPHATILAVGAGEERVVVKNKETVIAN
HHHCCCCHHHCCCCHHHHHCCCHHHHHHHHEECCCCEEEEEECCCCCEEEEECCCEEEEE
VMTVTLSTDHRCVDGALGAELLAAFKRYIESPMGMLV
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]