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The map label for this gene is pyrG [H]

Identifier: 150396290

GI number: 150396290

Start: 1136627

End: 1138255

Strand: Direct

Name: pyrG [H]

Synonym: Smed_1069

Alternate gene names: 150396290

Gene position: 1136627-1138255 (Clockwise)

Preceding gene: 150396289

Following gene: 150396291

Centisome position: 30.05

GC content: 61.69

Gene sequence:

>1629_bases
ATGGCGCGATATGTATTCATCACTGGCGGCGTGGTCTCCTCCCTCGGAAAAGGCATCGCTGCGGCTGCTCTCGGAGCATT
GCTGCAGGCGCGTGGCTACCGCGTGAGGCTGCGCAAACTCGATCCCTATCTGAACGTCGATCCGGGTACTATGAGCCCGA
CCCAGCACGGGGAGGTGTTCGTCACCGACGACGGGGCGGAAACGGACCTGGATCTCGGGCATTACGAGCGCTTCACGGGC
CGCTCGGCGACGAAGACCGACAACATCACCACGGGCCGGATCTATAAGAACATCATCGACAAGGAGCGGCGCGGCGACTA
TCTGGGCGCGACGGTCCAGGTGATTCCGCACGTCACGAACGAAATCAAGAACTTCGTCACCGAAGGCAATGAGGATTACG
ACTTCGTCATTTGCGAGATCGGCGGCACCGTCGGCGACATCGAGGCGATGCCGTTCATGGAAGCGATCCGCCAGCTCGGT
AACGACCTGCCGCGCGGCACCGCCGTTTATGTGCACCTGACGCTGATGCCTTATATCCCGGCGGCGGGCGAACTGAAGAC
CAAGCCGACCCAGCATTCGGTCAAGGAACTGCAGGCGCTCGGTATTCACCCGGATATCCTGCTCGTGCGGGCCGACCGAG
AAATCCCGGAAGCGGAGCGCCGCAAGCTGTCGCTCTTCTGCAATGTCCGCCAGTCCGCCGTCATCCAGGCGCTCGACGTC
GCTTCTATCTACGACGTGCCGATCGCCTATCACAAGGAAGGGCTCGACAACGAGGTGCTTGCCGCTTTCGGCATCGAGCC
GGCGCCGAAGCCGCGCATGGAGGCGTGGGAAGATGTTGCACATCGTATCCGCACGCCGGAAGGCGAGGTCACGATTGCAA
TCGTCGGCAAGTATACCGGTCTCAAGGACGCCTATAAATCGCTGATCGAGGCGCTTTACCATGGCGGCATCGCCAACCGG
GTCAAGGTCAAGCTCGAGTGGATCGAGTCGGAGGTCTTCGAAAAGGAGGACCCGGCACCCTATCTGGAAAAAGTCCACGG
CATTCTCGTTCCCGGCGGGTTCGGCGAACGCGGTTCGGAGGGCAAGATCAATGCGGCGCGTTTCGCCCGCGAGCGCAAGG
TGCCCTATTTCGGCATCTGCTTCGGCATGCAGATGGCGGTCGTCGAAGCGGCGCGCAACCTCGCCGGTATCGAAAAGGCA
TCTTCTACGGAATTCGGCCCGACCAAGGAACCGGTCGTCGGCCTGATGACCGAGTGGGTGAAGGGCAATGAACTCGAGAA
GCGTTCCGCCGCGGGCGATCTCGGCGGCACGATGCGCCTCGGCGCCTATCGAGCGGCCCTGAAGCCTGAAACCAAGATCG
CCGGTATCTATGGCTCGCCGGATATTTCCGAGCGTCACCGCCACCGCTACGAAGTGAATGTCGATTACAAGGACCGGCTG
GAATCCTGCGGCCTCGTCTTTTCGGGCATGTCGCCGGACGGCGTTCTGCCGGAGACGGTCGAGTATCCCGACCATCCCTG
GTTCATCGGCGTCCAGTACCATCCGGAACTGAAGTCGCGCCCGCTCGATCCCCACCCGCTCTTTGCGAGTTTCATCGAGG
CAGCACTCGAACAGTCGCGGCTCGTTTAG

Upstream 100 bases:

>100_bases
GGTTTTATTTTGTGTGAAATCTGCCATTCCGCACGGAAAATTCTAAAAAAGGAATTTTTCGGTGGCGGAATCGTTTGTGA
AAAGGTATCCGGTGACTCCC

Downstream 100 bases:

>100_bases
AGCTGCCGTCGACGGCGGGGCGGGGCGGGCTCCCTGCCACGACGACGGCCGGCGACGCCCATCGGCCGGAGCCCGCTTGC
AATCGGCGGGCAAAAGTTGC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MARYVFITGGVVSSLGKGIAAAALGALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVFVTDDGAETDLDLGHYERFTG
RSATKTDNITTGRIYKNIIDKERRGDYLGATVQVIPHVTNEIKNFVTEGNEDYDFVICEIGGTVGDIEAMPFMEAIRQLG
NDLPRGTAVYVHLTLMPYIPAAGELKTKPTQHSVKELQALGIHPDILLVRADREIPEAERRKLSLFCNVRQSAVIQALDV
ASIYDVPIAYHKEGLDNEVLAAFGIEPAPKPRMEAWEDVAHRIRTPEGEVTIAIVGKYTGLKDAYKSLIEALYHGGIANR
VKVKLEWIESEVFEKEDPAPYLEKVHGILVPGGFGERGSEGKINAARFARERKVPYFGICFGMQMAVVEAARNLAGIEKA
SSTEFGPTKEPVVGLMTEWVKGNELEKRSAAGDLGGTMRLGAYRAALKPETKIAGIYGSPDISERHRHRYEVNVDYKDRL
ESCGLVFSGMSPDGVLPETVEYPDHPWFIGVQYHPELKSRPLDPHPLFASFIEAALEQSRLV

Sequences:

>Translated_542_residues
MARYVFITGGVVSSLGKGIAAAALGALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVFVTDDGAETDLDLGHYERFTG
RSATKTDNITTGRIYKNIIDKERRGDYLGATVQVIPHVTNEIKNFVTEGNEDYDFVICEIGGTVGDIEAMPFMEAIRQLG
NDLPRGTAVYVHLTLMPYIPAAGELKTKPTQHSVKELQALGIHPDILLVRADREIPEAERRKLSLFCNVRQSAVIQALDV
ASIYDVPIAYHKEGLDNEVLAAFGIEPAPKPRMEAWEDVAHRIRTPEGEVTIAIVGKYTGLKDAYKSLIEALYHGGIANR
VKVKLEWIESEVFEKEDPAPYLEKVHGILVPGGFGERGSEGKINAARFARERKVPYFGICFGMQMAVVEAARNLAGIEKA
SSTEFGPTKEPVVGLMTEWVKGNELEKRSAAGDLGGTMRLGAYRAALKPETKIAGIYGSPDISERHRHRYEVNVDYKDRL
ESCGLVFSGMSPDGVLPETVEYPDHPWFIGVQYHPELKSRPLDPHPLFASFIEAALEQSRLV
>Mature_541_residues
ARYVFITGGVVSSLGKGIAAAALGALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVFVTDDGAETDLDLGHYERFTGR
SATKTDNITTGRIYKNIIDKERRGDYLGATVQVIPHVTNEIKNFVTEGNEDYDFVICEIGGTVGDIEAMPFMEAIRQLGN
DLPRGTAVYVHLTLMPYIPAAGELKTKPTQHSVKELQALGIHPDILLVRADREIPEAERRKLSLFCNVRQSAVIQALDVA
SIYDVPIAYHKEGLDNEVLAAFGIEPAPKPRMEAWEDVAHRIRTPEGEVTIAIVGKYTGLKDAYKSLIEALYHGGIANRV
KVKLEWIESEVFEKEDPAPYLEKVHGILVPGGFGERGSEGKINAARFARERKVPYFGICFGMQMAVVEAARNLAGIEKAS
STEFGPTKEPVVGLMTEWVKGNELEKRSAAGDLGGTMRLGAYRAALKPETKIAGIYGSPDISERHRHRYEVNVDYKDRLE
SCGLVFSGMSPDGVLPETVEYPDHPWFIGVQYHPELKSRPLDPHPLFASFIEAALEQSRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=557, Percent_Identity=44.165170556553, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI28559085, Length=562, Percent_Identity=43.9501779359431, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI28559083, Length=562, Percent_Identity=43.9501779359431, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI221316689, Length=562, Percent_Identity=43.9501779359431, Blast_Score=457, Evalue=1e-128,
Organism=Escherichia coli, GI1789142, Length=536, Percent_Identity=56.5298507462687, Blast_Score=594, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=36.7816091954023, Blast_Score=393, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6322563, Length=570, Percent_Identity=40, Blast_Score=421, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6319432, Length=567, Percent_Identity=40.2116402116402, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24664469, Length=560, Percent_Identity=44.1071428571429, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI21357815, Length=506, Percent_Identity=43.4782608695652, Blast_Score=387, Evalue=1e-107,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59874; Mature: 59743

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYVFITGGVVSSLGKGIAAAALGALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVF
CCEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGRSATKTDNITTGRIYKNIIDKERRGDYLGATVQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EIKNFVTEGNEDYDFVICEIGGTVGDIEAMPFMEAIRQLGNDLPRGTAVYVHLTLMPYIP
HHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCC
AAGELKTKPTQHSVKELQALGIHPDILLVRADREIPEAERRKLSLFCNVRQSAVIQALDV
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHEEEHEECCHHHHHHHHHHH
ASIYDVPIAYHKEGLDNEVLAAFGIEPAPKPRMEAWEDVAHRIRTPEGEVTIAIVGKYTG
HHHHCCCHHHHHCCCCCCHHHEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCC
LKDAYKSLIEALYHGGIANRVKVKLEWIESEVFEKEDPAPYLEKVHGILVPGGFGERGSE
HHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHCCCCCCHHHHHHCEEEECCCCCCCCCC
GKINAARFARERKVPYFGICFGMQMAVVEAARNLAGIEKASSTEFGPTKEPVVGLMTEWV
CCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
KGNELEKRSAAGDLGGTMRLGAYRAALKPETKIAGIYGSPDISERHRHRYEVNVDYKDRL
CCCCCHHHHCCCCCCCCEEHHHHHHHCCCCCEEEEEECCCCCCHHHCCEEEEECCHHHHH
ESCGLVFSGMSPDGVLPETVEYPDHPWFIGVQYHPELKSRPLDPHPLFASFIEAALEQSR
HHCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCHHHHHHHHHHHHHHC
LV
CC
>Mature Secondary Structure 
ARYVFITGGVVSSLGKGIAAAALGALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVF
CEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGRSATKTDNITTGRIYKNIIDKERRGDYLGATVQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EIKNFVTEGNEDYDFVICEIGGTVGDIEAMPFMEAIRQLGNDLPRGTAVYVHLTLMPYIP
HHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCC
AAGELKTKPTQHSVKELQALGIHPDILLVRADREIPEAERRKLSLFCNVRQSAVIQALDV
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHEEEHEECCHHHHHHHHHHH
ASIYDVPIAYHKEGLDNEVLAAFGIEPAPKPRMEAWEDVAHRIRTPEGEVTIAIVGKYTG
HHHHCCCHHHHHCCCCCCHHHEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCC
LKDAYKSLIEALYHGGIANRVKVKLEWIESEVFEKEDPAPYLEKVHGILVPGGFGERGSE
HHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHCCCCCCHHHHHHCEEEECCCCCCCCCC
GKINAARFARERKVPYFGICFGMQMAVVEAARNLAGIEKASSTEFGPTKEPVVGLMTEWV
CCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
KGNELEKRSAAGDLGGTMRLGAYRAALKPETKIAGIYGSPDISERHRHRYEVNVDYKDRL
CCCCCHHHHCCCCCCCCEEHHHHHHHCCCCCEEEEEECCCCCCHHHCCEEEEECCHHHHH
ESCGLVFSGMSPDGVLPETVEYPDHPWFIGVQYHPELKSRPLDPHPLFASFIEAALEQSR
HHCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCHHHHHHHHHHHHHHC
LV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]