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The map label for this gene is paaG [C]

Identifier: 150395713

GI number: 150395713

Start: 530441

End: 531193

Strand: Direct

Name: paaG [C]

Synonym: Smed_0488

Alternate gene names: 150395713

Gene position: 530441-531193 (Clockwise)

Preceding gene: 150395712

Following gene: 150395714

Centisome position: 14.03

GC content: 64.94

Gene sequence:

>753_bases
ATGACCGACCATGTGCTCGTTGAACGGCCGGAAGCCTATCCCGGAGTCCAGCTCATCCGCTTCAACCGGCCGGAGAAGAA
GAACGCCATCACGCGCGAAATGTACGCCAAAATGACGGAAGCGCTGACCGCTGCCGGGGCGGACCCCGCCATTCGCGCGA
CGGCATTTCTCGGAACGCAGGGCTGCTTCTCGGCCGGCAACGACATGTCGGACTTCCTCGCATTCGCCATGGGGGGAAGT
ATGGGAGGGGAGGTTCTTGATTTTCTCCGGGCTCTTGCGGGTGCGACGAAGCCGGTCGTCTCGGGCGTCGACGGATTGGC
CATCGGCATCGGAACGACGATCCACCTCCACTGCGACCTGACGGTCTCCTCCGCCCGTTCGGTCTTCAAGACTCCCTTCG
TCGACCTGGCGCTCGTTCCGGAAGCTGCCTCGAGCCTCATCGCTCCACGCATCATGGGTCATCAGCGCGCCTTTGCCCTG
CTTGCAGCCGGCGAACCGCTCGACGCCGCCGGTGCGCGCGAGGCGGGGCTGATCTGGAAGATCGTGGATGAGCCTGCCGT
GGAGGAGGAAACGCTTTCCCTTGCCGGGCGCCTGGCAAGGAAGCCGCCGGAAGCGCTGCGCATCGCCCGCGATCTCGTTC
GCGGCGACCGCAGCGATGTGCTTGCCCGCATCGAGGAGGAGGCGAAGCATTTTGCCGCGCAGTTGAAGAGTGCGGAAGCA
CGGGCCGCGTTCGAAGCCTTCATGCGTCGGTAG

Upstream 100 bases:

>100_bases
GAAAACCTGGTTTCGGAGACGAGTGCACTCGAGGACCGCGTAACCAACGGCGCGGAAAGTCTCGCCGCTGCCCGTTCCAT
ACTCGATTGAGGGAAGATCC

Downstream 100 bases:

>100_bases
GACGGGATAACGTCGAACTCTCCTACGGTGGTGTGTCGAGCGAATTCGAGTAAGTGCGACGCAGCACCCTTCGTCGTCCG
CGGCTTTCCCCTCGTCATCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MTDHVLVERPEAYPGVQLIRFNRPEKKNAITREMYAKMTEALTAAGADPAIRATAFLGTQGCFSAGNDMSDFLAFAMGGS
MGGEVLDFLRALAGATKPVVSGVDGLAIGIGTTIHLHCDLTVSSARSVFKTPFVDLALVPEAASSLIAPRIMGHQRAFAL
LAAGEPLDAAGAREAGLIWKIVDEPAVEEETLSLAGRLARKPPEALRIARDLVRGDRSDVLARIEEEAKHFAAQLKSAEA
RAAFEAFMRR

Sequences:

>Translated_250_residues
MTDHVLVERPEAYPGVQLIRFNRPEKKNAITREMYAKMTEALTAAGADPAIRATAFLGTQGCFSAGNDMSDFLAFAMGGS
MGGEVLDFLRALAGATKPVVSGVDGLAIGIGTTIHLHCDLTVSSARSVFKTPFVDLALVPEAASSLIAPRIMGHQRAFAL
LAAGEPLDAAGAREAGLIWKIVDEPAVEEETLSLAGRLARKPPEALRIARDLVRGDRSDVLARIEEEAKHFAAQLKSAEA
RAAFEAFMRR
>Mature_249_residues
TDHVLVERPEAYPGVQLIRFNRPEKKNAITREMYAKMTEALTAAGADPAIRATAFLGTQGCFSAGNDMSDFLAFAMGGSM
GGEVLDFLRALAGATKPVVSGVDGLAIGIGTTIHLHCDLTVSSARSVFKTPFVDLALVPEAASSLIAPRIMGHQRAFALL
AAGEPLDAAGAREAGLIWKIVDEPAVEEETLSLAGRLARKPPEALRIARDLVRGDRSDVLARIEEEAKHFAAQLKSAEAR
AAFEAFMRR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=243, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI260275230, Length=243, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI260274832, Length=243, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=23.6947791164659, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI25453481, Length=254, Percent_Identity=25.5905511811024, Blast_Score=87, Evalue=9e-18,
Organism=Homo sapiens, GI51538573, Length=254, Percent_Identity=25.5905511811024, Blast_Score=87, Evalue=9e-18,
Organism=Homo sapiens, GI4757966, Length=249, Percent_Identity=25.7028112449799, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI51538581, Length=249, Percent_Identity=25.7028112449799, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4757968, Length=254, Percent_Identity=25.5905511811024, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI49355787, Length=254, Percent_Identity=25.5905511811024, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI68989263, Length=197, Percent_Identity=31.4720812182741, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI116235487, Length=241, Percent_Identity=24.896265560166, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI221136756, Length=235, Percent_Identity=23.4042553191489, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI221136753, Length=235, Percent_Identity=23.4042553191489, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI20127408, Length=175, Percent_Identity=25.1428571428571, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI221307494, Length=200, Percent_Identity=24.5, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI31542718, Length=189, Percent_Identity=26.4550264550265, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1787660, Length=241, Percent_Identity=30.2904564315353, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787659, Length=236, Percent_Identity=25.8474576271186, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=27.2357723577236, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=242, Percent_Identity=29.3388429752066, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17549921, Length=230, Percent_Identity=30, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=25.1012145748988, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=233, Percent_Identity=24.4635193133047, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=247, Percent_Identity=25.5060728744939, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=223, Percent_Identity=26.9058295964126, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=266, Percent_Identity=25.187969924812, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24654903, Length=240, Percent_Identity=32.0833333333333, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=22.0883534136546, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=22.0883534136546, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21357171, Length=222, Percent_Identity=27.9279279279279, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653139, Length=220, Percent_Identity=26.8181818181818, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24650670, Length=224, Percent_Identity=27.6785714285714, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19920382, Length=214, Percent_Identity=24.2990654205607, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 26686; Mature: 26555

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHVLVERPEAYPGVQLIRFNRPEKKNAITREMYAKMTEALTAAGADPAIRATAFLGTQ
CCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
GCFSAGNDMSDFLAFAMGGSMGGEVLDFLRALAGATKPVVSGVDGLAIGIGTTIHLHCDL
CHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHCCCCCEEEEECCEEEEEEEE
TVSSARSVFKTPFVDLALVPEAASSLIAPRIMGHQRAFALLAAGEPLDAAGAREAGLIWK
EHHHHHHHHHCCCHHEEECHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEE
IVDEPAVEEETLSLAGRLARKPPEALRIARDLVRGDRSDVLARIEEEAKHFAAQLKSAEA
ECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
RAAFEAFMRR
HHHHHHHHCC
>Mature Secondary Structure 
TDHVLVERPEAYPGVQLIRFNRPEKKNAITREMYAKMTEALTAAGADPAIRATAFLGTQ
CCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
GCFSAGNDMSDFLAFAMGGSMGGEVLDFLRALAGATKPVVSGVDGLAIGIGTTIHLHCDL
CHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHCCCCCEEEEECCEEEEEEEE
TVSSARSVFKTPFVDLALVPEAASSLIAPRIMGHQRAFALLAAGEPLDAAGAREAGLIWK
EHHHHHHHHHCCCHHEEECHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEE
IVDEPAVEEETLSLAGRLARKPPEALRIARDLVRGDRSDVLARIEEEAKHFAAQLKSAEA
ECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
RAAFEAFMRR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA