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The map label for this gene is glcD [H]

Identifier: 150395619

GI number: 150395619

Start: 422685

End: 424124

Strand: Direct

Name: glcD [H]

Synonym: Smed_0394

Alternate gene names: 150395619

Gene position: 422685-424124 (Clockwise)

Preceding gene: 150395617

Following gene: 150395620

Centisome position: 11.18

GC content: 60.83

Gene sequence:

>1440_bases
ATGCCGGAGACGATAGGCTTTCTGAAGCCCAGGCAGGCTGTTCTCGATCGTCGGCGGGAAATCGTCGCCGACCTCGGTGA
TCTGCTTCCGGAAGGTTGCCTCATCAGCGACGAACGTGGCCTCAAGCCATTCGAGACGGATGCATTTATCGCCTACCGGC
GGATGCCGCTCGCGGTCGTTCTCCCCGAGACGACGGAACAGGTGGCCACGGTCCTCAAATACTGTAGCCGCTACGGTATT
CCAATTGTCCCGAGGGGTGCCGGCACCTCCCTCTCCGGCGGGGCCATTCCTCAGGAAGACGCGATCGTCGTCGGCCTGTC
GAAGATGGCGCGCATCCTCGATATCGACCTCTTCAACCGGACGGCGACGGTTCAGGCGGGCGTGACCAATCTCAACATTT
CCGACGCGGTTTCGGCCGATGGATTCTTCTATGCGCCGGATCCGAGTTCGCAGCTCGCCTGCACCATCGGCGGGAATATC
GGGATGAATTCCGGTGGCGCGCACTGTCTGAAATACGGGGTGACCACAAACAACCTTCTTGGCGTCAAGATGGTGCTTTT
CGACGGCACCGTGATCGAACTTGGTGGCAAGGCGCTCGACGCCTCGGGATATGACTTGCTTGGCCTTATCTGTGGTTCCG
AGGGGCAGCTTGGTATCGTTACGGAAGCGACGGTGCGGCTGATTGCCAAGGCCGAAGGCGCGCGCCCCGTTCTTTTCGGA
TTTGCCACTTCAGAATCCGCCGGGAGCTGCGTCGCCGATATCATCGGATCGGGCATCATTCCGGTTGCGATCGAATTCAT
GGACAAACCGGCAATCGAAATCTGCGAAGCCTTCGCGCATGCGGGGTACCCGCTCGACGTCGAAGCATTGCTGATCGTCG
AAGTGGAAGGTTCCGAGGCTGAAATGGATTCGATGCTCGCAGGCATCATCGGGATTGCCCGCCGCCACGGTGTGATGACG
ATCAAGGAATCGCAATCGGCGCTCGAAGCGGCACTGATCTGGAAGGGGCGGAAATCGGCCTTCGGCGCTACCGGCCGGAT
TGCCGATTATATCTGCATGGACGGAACGGTGCCGCTGAGCCAGCTCTCCCATGTCCTGCGCCGGACCGGCGAGATCGTCG
CCGGCTATGGCCTGCGCGTTGCCAATGTCTTCCATGCAGGTGACGGCAACATGCATCCGCTGATCCTCTACAATATCAAC
GATCCGGATGAAGCGGCCCGGGCGGAAGAGGCGGGCAACGACATTCTGAAGCTCTGCGTCGAGGCAGGCGGCTGCCTCAC
CGGGGAACACGGGGTCGGCATCGAGAAACGTGACCTGATGCTGTTTCAGTACAGCAAGGCCGATCTCGACCAGCAGATGG
GCGCGCGTGCTGCCTTCGATCCGCAATGGCTCCTGAACCCGTCGAAGGTCTTCCCGCTCGAAGGGCGGCCCGCGGCATGA

Upstream 100 bases:

>100_bases
TGATCATTGTTTCCAAAGGCGAAAAGATCATTAACATTTGCCGGGGGTCGGTCCTAGTGGTAAGCAAAAAATACCACTTT
GCTGCGAATGGGAGGCCCTC

Downstream 100 bases:

>100_bases
TCGTCCATTTCGACCCGGCAAGCGAAGAAGGGATTGCCTCCGTCGTTCGTTCAGCGGCGGCCGAGCGCGTCACGCTCGCG
GTCGTCGGTGGAGGCACGCG

Product: D-lactate dehydrogenase (cytochrome)

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MPETIGFLKPRQAVLDRRREIVADLGDLLPEGCLISDERGLKPFETDAFIAYRRMPLAVVLPETTEQVATVLKYCSRYGI
PIVPRGAGTSLSGGAIPQEDAIVVGLSKMARILDIDLFNRTATVQAGVTNLNISDAVSADGFFYAPDPSSQLACTIGGNI
GMNSGGAHCLKYGVTTNNLLGVKMVLFDGTVIELGGKALDASGYDLLGLICGSEGQLGIVTEATVRLIAKAEGARPVLFG
FATSESAGSCVADIIGSGIIPVAIEFMDKPAIEICEAFAHAGYPLDVEALLIVEVEGSEAEMDSMLAGIIGIARRHGVMT
IKESQSALEAALIWKGRKSAFGATGRIADYICMDGTVPLSQLSHVLRRTGEIVAGYGLRVANVFHAGDGNMHPLILYNIN
DPDEAARAEEAGNDILKLCVEAGGCLTGEHGVGIEKRDLMLFQYSKADLDQQMGARAAFDPQWLLNPSKVFPLEGRPAA

Sequences:

>Translated_479_residues
MPETIGFLKPRQAVLDRRREIVADLGDLLPEGCLISDERGLKPFETDAFIAYRRMPLAVVLPETTEQVATVLKYCSRYGI
PIVPRGAGTSLSGGAIPQEDAIVVGLSKMARILDIDLFNRTATVQAGVTNLNISDAVSADGFFYAPDPSSQLACTIGGNI
GMNSGGAHCLKYGVTTNNLLGVKMVLFDGTVIELGGKALDASGYDLLGLICGSEGQLGIVTEATVRLIAKAEGARPVLFG
FATSESAGSCVADIIGSGIIPVAIEFMDKPAIEICEAFAHAGYPLDVEALLIVEVEGSEAEMDSMLAGIIGIARRHGVMT
IKESQSALEAALIWKGRKSAFGATGRIADYICMDGTVPLSQLSHVLRRTGEIVAGYGLRVANVFHAGDGNMHPLILYNIN
DPDEAARAEEAGNDILKLCVEAGGCLTGEHGVGIEKRDLMLFQYSKADLDQQMGARAAFDPQWLLNPSKVFPLEGRPAA
>Mature_478_residues
PETIGFLKPRQAVLDRRREIVADLGDLLPEGCLISDERGLKPFETDAFIAYRRMPLAVVLPETTEQVATVLKYCSRYGIP
IVPRGAGTSLSGGAIPQEDAIVVGLSKMARILDIDLFNRTATVQAGVTNLNISDAVSADGFFYAPDPSSQLACTIGGNIG
MNSGGAHCLKYGVTTNNLLGVKMVLFDGTVIELGGKALDASGYDLLGLICGSEGQLGIVTEATVRLIAKAEGARPVLFGF
ATSESAGSCVADIIGSGIIPVAIEFMDKPAIEICEAFAHAGYPLDVEALLIVEVEGSEAEMDSMLAGIIGIARRHGVMTI
KESQSALEAALIWKGRKSAFGATGRIADYICMDGTVPLSQLSHVLRRTGEIVAGYGLRVANVFHAGDGNMHPLILYNIND
PDEAARAEEAGNDILKLCVEAGGCLTGEHGVGIEKRDLMLFQYSKADLDQQMGARAAFDPQWLLNPSKVFPLEGRPAA

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=423, Percent_Identity=29.3144208037825, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI37595754, Length=446, Percent_Identity=27.8026905829596, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI119964728, Length=448, Percent_Identity=27.0089285714286, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI4501993, Length=237, Percent_Identity=28.6919831223629, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1789351, Length=462, Percent_Identity=47.4025974025974, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1787977, Length=511, Percent_Identity=24.853228962818, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI48994907, Length=498, Percent_Identity=26.1044176706827, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71992373, Length=477, Percent_Identity=27.6729559748428, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71992381, Length=481, Percent_Identity=26.8191268191268, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI71992392, Length=363, Percent_Identity=27.2727272727273, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17534361, Length=432, Percent_Identity=27.5462962962963, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17556096, Length=220, Percent_Identity=25.9090909090909, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320027, Length=473, Percent_Identity=30.2325581395349, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6320764, Length=454, Percent_Identity=26.6519823788546, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320023, Length=462, Percent_Identity=25.1082251082251, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI18921117, Length=430, Percent_Identity=26.7441860465116, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24639277, Length=430, Percent_Identity=26.7441860465116, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24639275, Length=430, Percent_Identity=26.7441860465116, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24653753, Length=221, Percent_Identity=24.8868778280543, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 50713; Mature: 50582

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETIGFLKPRQAVLDRRREIVADLGDLLPEGCLISDERGLKPFETDAFIAYRRMPLAVV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEEECCCEEEE
LPETTEQVATVLKYCSRYGIPIVPRGAGTSLSGGAIPQEDAIVVGLSKMARILDIDLFNR
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCC
TATVQAGVTNLNISDAVSADGFFYAPDPSSQLACTIGGNIGMNSGGAHCLKYGVTTNNLL
EEEEEECCEECCCHHCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCE
GVKMVLFDGTVIELGGKALDASGYDLLGLICGSEGQLGIVTEATVRLIAKAEGARPVLFG
EEEEEEECCEEEEECCCEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHHCCCCCEEEEE
FATSESAGSCVADIIGSGIIPVAIEFMDKPAIEICEAFAHAGYPLDVEALLIVEVEGSEA
EECCCCCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEEECCCHH
EMDSMLAGIIGIARRHGVMTIKESQSALEAALIWKGRKSAFGATGRIADYICMDGTVPLS
HHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCHHEEEEECCCCCHH
QLSHVLRRTGEIVAGYGLRVANVFHAGDGNMHPLILYNINDPDEAARAEEAGNDILKLCV
HHHHHHHHCCCEEECCCEEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHCHHHHHHHH
EAGGCLTGEHGVGIEKRDLMLFQYSKADLDQQMGARAAFDPQWLLNPSKVFPLEGRPAA
HCCCEEECCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCCCHHCCCCCCEECCCCCCCC
>Mature Secondary Structure 
PETIGFLKPRQAVLDRRREIVADLGDLLPEGCLISDERGLKPFETDAFIAYRRMPLAVV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEEECCCEEEE
LPETTEQVATVLKYCSRYGIPIVPRGAGTSLSGGAIPQEDAIVVGLSKMARILDIDLFNR
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCC
TATVQAGVTNLNISDAVSADGFFYAPDPSSQLACTIGGNIGMNSGGAHCLKYGVTTNNLL
EEEEEECCEECCCHHCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCE
GVKMVLFDGTVIELGGKALDASGYDLLGLICGSEGQLGIVTEATVRLIAKAEGARPVLFG
EEEEEEECCEEEEECCCEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHHCCCCCEEEEE
FATSESAGSCVADIIGSGIIPVAIEFMDKPAIEICEAFAHAGYPLDVEALLIVEVEGSEA
EECCCCCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEEECCCHH
EMDSMLAGIIGIARRHGVMTIKESQSALEAALIWKGRKSAFGATGRIADYICMDGTVPLS
HHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCHHEEEEECCCCCHH
QLSHVLRRTGEIVAGYGLRVANVFHAGDGNMHPLILYNINDPDEAARAEEAGNDILKLCV
HHHHHHHHCCCEEECCCEEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHCHHHHHHHH
EAGGCLTGEHGVGIEKRDLMLFQYSKADLDQQMGARAAFDPQWLLNPSKVFPLEGRPAA
HCCCEEECCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCCCHHCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]