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The map label for this gene is potH [H]

Identifier: 150395539

GI number: 150395539

Start: 336399

End: 337310

Strand: Direct

Name: potH [H]

Synonym: Smed_0312

Alternate gene names: 150395539

Gene position: 336399-337310 (Clockwise)

Preceding gene: 150395538

Following gene: 150395540

Centisome position: 8.89

GC content: 58.33

Gene sequence:

>912_bases
ATGGCGAAACTTGCCTCAGCCTTTGTCAGCCGCCTGGTCATCATCATTCCTTATGCCTGGCTGCTGTTCTTCTTCCTGAT
CCCCTTCTTCATCGTCTTCCGCATTTCGCTGTCGCAGACGGCCGTGGCGATGCCGCCCTACATGCCGGTCTTCGACCTGG
CGGGCGGCCTTTCCGGTATTGCGCAGAAGCTGCGGGAATTCTCTCTCGACAATTACGTATGGCTGACGGAGGACGCGCTC
TATTTCAACGCTTACGTGTCCAGTGTCGTTATCGCCGCGATCTCGACCTTTCTGACGCTCCTGATTGGATATCCGATTGC
CTACGGCATGGCGAAGGCGCCGCGCTCGTTGCGGCCCACGCTGCTGATGATGGTGATCCTGCCGTTCTGGACGAGCTTTC
TGATCCGCGTCTATGCCTGGATCGCCATCCTGAAACCGGAGGGCCTGCTCAATCAGTTTTTATCGACCCTCGGCCTGATC
GATCAGCCGCTGATTATTCTCAATACCAACTGGGCGATCTATATCGGCATCGTCTATTCCTATCTCCCCTTCATGGTGCT
GCCGATCTACTCGGCTCTGGAGAAGATGGACCTTTCGCTGACGGAGGCTGCGCAGGATCTCGGCTGCACGCCGGCCGCCG
CGTTCTGGCGCGTGACCTTTCCCCTGTCCCTGCCCGGCGTCGTCGCGGGCTGCCTGCTCGTATTCATTCCCGCCGTCGGC
GAATTCGTCATTCCCGATCTCCTCGGCGGTTCGGAGACGCTGATGATCGGCAAGACTTTGTGGAGCGAGTTCAACTCCAA
CCGCGATTGGCCCGTATCGTCGGCAGTGGCGATCATCCTGCTGATGATCCTGGTGATACCGATCGTATATTTCCAGAACA
TCCAGGCCAAAGCCGACGGCGAGGGAAGGTGA

Upstream 100 bases:

>100_bases
CTGAACGCCGTGCGGGCGGTCGAGGATCCTCTTGGCTATGACCAGCAGGTCTGGGTCTCCTTCGGTGAAGATGCAGGCGT
CGTATTGAAGGATTGAAGCC

Downstream 100 bases:

>100_bases
GACATGGAAAAGTGGTCACGATTCAATATCGCCTCCGTCGTTCTCGGCTTCGGCTTTCTCTATCTGCCGATCGTGCTCCT
GGTGATCTTCTCCTTCAACG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MAKLASAFVSRLVIIIPYAWLLFFFLIPFFIVFRISLSQTAVAMPPYMPVFDLAGGLSGIAQKLREFSLDNYVWLTEDAL
YFNAYVSSVVIAAISTFLTLLIGYPIAYGMAKAPRSLRPTLLMMVILPFWTSFLIRVYAWIAILKPEGLLNQFLSTLGLI
DQPLIILNTNWAIYIGIVYSYLPFMVLPIYSALEKMDLSLTEAAQDLGCTPAAAFWRVTFPLSLPGVVAGCLLVFIPAVG
EFVIPDLLGGSETLMIGKTLWSEFNSNRDWPVSSAVAIILLMILVIPIVYFQNIQAKADGEGR

Sequences:

>Translated_303_residues
MAKLASAFVSRLVIIIPYAWLLFFFLIPFFIVFRISLSQTAVAMPPYMPVFDLAGGLSGIAQKLREFSLDNYVWLTEDAL
YFNAYVSSVVIAAISTFLTLLIGYPIAYGMAKAPRSLRPTLLMMVILPFWTSFLIRVYAWIAILKPEGLLNQFLSTLGLI
DQPLIILNTNWAIYIGIVYSYLPFMVLPIYSALEKMDLSLTEAAQDLGCTPAAAFWRVTFPLSLPGVVAGCLLVFIPAVG
EFVIPDLLGGSETLMIGKTLWSEFNSNRDWPVSSAVAIILLMILVIPIVYFQNIQAKADGEGR
>Mature_302_residues
AKLASAFVSRLVIIIPYAWLLFFFLIPFFIVFRISLSQTAVAMPPYMPVFDLAGGLSGIAQKLREFSLDNYVWLTEDALY
FNAYVSSVVIAAISTFLTLLIGYPIAYGMAKAPRSLRPTLLMMVILPFWTSFLIRVYAWIAILKPEGLLNQFLSTLGLID
QPLIILNTNWAIYIGIVYSYLPFMVLPIYSALEKMDLSLTEAAQDLGCTPAAAFWRVTFPLSLPGVVAGCLLVFIPAVGE
FVIPDLLGGSETLMIGKTLWSEFNSNRDWPVSSAVAIILLMILVIPIVYFQNIQAKADGEGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=301, Percent_Identity=56.1461794019934, Blast_Score=308, Evalue=2e-85,
Organism=Escherichia coli, GI1787369, Length=218, Percent_Identity=46.7889908256881, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1787713, Length=243, Percent_Identity=27.5720164609054, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33617; Mature: 33485

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLASAFVSRLVIIIPYAWLLFFFLIPFFIVFRISLSQTAVAMPPYMPVFDLAGGLSGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHH
AQKLREFSLDNYVWLTEDALYFNAYVSSVVIAAISTFLTLLIGYPIAYGMAKAPRSLRPT
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LLMMVILPFWTSFLIRVYAWIAILKPEGLLNQFLSTLGLIDQPLIILNTNWAIYIGIVYS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
YLPFMVLPIYSALEKMDLSLTEAAQDLGCTPAAAFWRVTFPLSLPGVVAGCLLVFIPAVG
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHH
EFVIPDLLGGSETLMIGKTLWSEFNSNRDWPVSSAVAIILLMILVIPIVYFQNIQAKADG
HHHHHHHCCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EGR
CCC
>Mature Secondary Structure 
AKLASAFVSRLVIIIPYAWLLFFFLIPFFIVFRISLSQTAVAMPPYMPVFDLAGGLSGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHH
AQKLREFSLDNYVWLTEDALYFNAYVSSVVIAAISTFLTLLIGYPIAYGMAKAPRSLRPT
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LLMMVILPFWTSFLIRVYAWIAILKPEGLLNQFLSTLGLIDQPLIILNTNWAIYIGIVYS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
YLPFMVLPIYSALEKMDLSLTEAAQDLGCTPAAAFWRVTFPLSLPGVVAGCLLVFIPAVG
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHH
EFVIPDLLGGSETLMIGKTLWSEFNSNRDWPVSSAVAIILLMILVIPIVYFQNIQAKADG
HHHHHHHCCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EGR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]