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The map label for this gene is folD1

Identifier: 150395517

GI number: 150395517

Start: 308111

End: 309010

Strand: Direct

Name: folD1

Synonym: Smed_0290

Alternate gene names: 150395517

Gene position: 308111-309010 (Clockwise)

Preceding gene: 150395515

Following gene: 150395519

Centisome position: 8.15

GC content: 62.89

Gene sequence:

>900_bases
ATGACGACTGTGATTGATGGCAAGGATGTTGCTGCTTCGGTCATCGAAACCGTAAAAACCGCCACCAGCACGCTGGAACG
CGAAACGGGCGTGCGCGCGGGCCTCGCGGTCGTCATTGTCGGGGACGATCCGGCAAGCCACACTTATGTTTCGGCGAAGA
GCAGGATGGCCAAGGAATGCGGCTTTCTGTCCGTCCAGCATACGCTGCCGGAGGAGACGTCGCAGGAGGAACTGGCGGCG
CTTGTCGCTCAGCTCAATGCGGACGCGGCCATCCACGGCATTCTCGTGCAACTGCCTTTGCCGAAGCATCTCCGATCGGA
GCCGATCATCCAATCGATCCTCCCCGAGAAGGACGTCGACGGCCTGCATGTGGTCAATGCGGGCAAGCTTGCAACCGGCG
ACCTCGAAGGTGGGCTGGTCTCCTGCACGCCGGCCGGTGCGATGGTTTTCGTGCGCCGGACGTATGGCGACGACCTCTCC
GGACTCAGCGCGGTCGTGATCGGCCGCTCCAACCTCTTCGGCAAGCCGATGGGTGCCCTGCTCCTCGCAGCCAATGCGAC
GGTTACGACGGCGCATTCGCGCACCAAGAACCTTCCGGAAGTTTGCCGAAATGCGGATATTCTCGTTGCTGCGGTCGGTC
GTCCGGAGATGGTCAAGGCGGACTGGGTCAAGCCCGGTGCGATGGTGATCGACGTCGGCATCAACCGCGTTCCTGCGCCG
GAGCGAGGCGAGGGTAAGACCAAGCTCGTCGGTGATGTCGCTTTCAAGGAGTGCGCCGAGCTCGCAAGCGTGATTACGCC
TGTGCCGGGCGGCGTCGGACCCATGACGATCGCCATGCTGATGGCGAATACGATCATCGCCGCCCATCGCAAAGCCGGCC
GTCGGCCGCCGAAGTTCTGA

Upstream 100 bases:

>100_bases
GCAATTATGCGTTTGGAACAGCATAGGGCGCATGCAGGCAATCGCCGGATGCGGCTTGAATTAGAGTGTGCCGCGTTCGG
GACAAATCGGGAGATACCCC

Downstream 100 bases:

>100_bases
CGGCTGCCGCGCGACGCCGCACGACGCTACACAGTTTTCCCTGAATTGCGCTGGCGATCGGGTGCGGGCCCGGTCGAGGC
TCGCACGATCAATTCGGCGC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTTVIDGKDVAASVIETVKTATSTLERETGVRAGLAVVIVGDDPASHTYVSAKSRMAKECGFLSVQHTLPEETSQEELAA
LVAQLNADAAIHGILVQLPLPKHLRSEPIIQSILPEKDVDGLHVVNAGKLATGDLEGGLVSCTPAGAMVFVRRTYGDDLS
GLSAVVIGRSNLFGKPMGALLLAANATVTTAHSRTKNLPEVCRNADILVAAVGRPEMVKADWVKPGAMVIDVGINRVPAP
ERGEGKTKLVGDVAFKECAELASVITPVPGGVGPMTIAMLMANTIIAAHRKAGRRPPKF

Sequences:

>Translated_299_residues
MTTVIDGKDVAASVIETVKTATSTLERETGVRAGLAVVIVGDDPASHTYVSAKSRMAKECGFLSVQHTLPEETSQEELAA
LVAQLNADAAIHGILVQLPLPKHLRSEPIIQSILPEKDVDGLHVVNAGKLATGDLEGGLVSCTPAGAMVFVRRTYGDDLS
GLSAVVIGRSNLFGKPMGALLLAANATVTTAHSRTKNLPEVCRNADILVAAVGRPEMVKADWVKPGAMVIDVGINRVPAP
ERGEGKTKLVGDVAFKECAELASVITPVPGGVGPMTIAMLMANTIIAAHRKAGRRPPKF
>Mature_298_residues
TTVIDGKDVAASVIETVKTATSTLERETGVRAGLAVVIVGDDPASHTYVSAKSRMAKECGFLSVQHTLPEETSQEELAAL
VAQLNADAAIHGILVQLPLPKHLRSEPIIQSILPEKDVDGLHVVNAGKLATGDLEGGLVSCTPAGAMVFVRRTYGDDLSG
LSAVVIGRSNLFGKPMGALLLAANATVTTAHSRTKNLPEVCRNADILVAAVGRPEMVKADWVKPGAMVIDVGINRVPAPE
RGEGKTKLVGDVAFKECAELASVITPVPGGVGPMTIAMLMANTIIAAHRKAGRRPPKF

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=297, Percent_Identity=45.7912457912458, Blast_Score=258, Evalue=5e-69,
Organism=Homo sapiens, GI222418558, Length=297, Percent_Identity=42.4242424242424, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI94721354, Length=297, Percent_Identity=42.0875420875421, Blast_Score=193, Evalue=1e-49,
Organism=Homo sapiens, GI36796743, Length=223, Percent_Identity=26.9058295964126, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1786741, Length=286, Percent_Identity=48.6013986013986, Blast_Score=240, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI17568735, Length=293, Percent_Identity=43.0034129692833, Blast_Score=217, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=43.2525951557093, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=41.0774410774411, Blast_Score=216, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6322933, Length=309, Percent_Identity=25.5663430420712, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=45.8620689655172, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=45.8620689655172, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=45.8620689655172, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=45.8620689655172, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI17136818, Length=305, Percent_Identity=44.2622950819672, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI17136816, Length=305, Percent_Identity=44.2622950819672, Blast_Score=218, Evalue=6e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD1_SINMW (A6U669)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001325984.1
- ProteinModelPortal:   A6U669
- SMR:   A6U669
- STRING:   A6U669
- GeneID:   5321122
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_0290
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- ProtClustDB:   PRK14188
- BioCyc:   SMED366394:SMED_0290-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31229; Mature: 31097

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVIDGKDVAASVIETVKTATSTLERETGVRAGLAVVIVGDDPASHTYVSAKSRMAKEC
CCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHC
GFLSVQHTLPEETSQEELAALVAQLNADAAIHGILVQLPLPKHLRSEPIIQSILPEKDVD
CCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCHHHCCCHHHHHHCCCCCCC
GLHVVNAGKLATGDLEGGLVSCTPAGAMVFVRRTYGDDLSGLSAVVIGRSNLFGKPMGAL
CEEEEECCCEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHE
LLAANATVTTAHSRTKNLPEVCRNADILVAAVGRPEMVKADWVKPGAMVIDVGINRVPAP
EEECCCEEEECCHHCCCCHHHHCCCCEEEEECCCCCEEECCCCCCCCEEEEECCCCCCCC
ERGEGKTKLVGDVAFKECAELASVITPVPGGVGPMTIAMLMANTIIAAHRKAGRRPPKF
CCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTVIDGKDVAASVIETVKTATSTLERETGVRAGLAVVIVGDDPASHTYVSAKSRMAKEC
CEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHC
GFLSVQHTLPEETSQEELAALVAQLNADAAIHGILVQLPLPKHLRSEPIIQSILPEKDVD
CCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCHHHCCCHHHHHHCCCCCCC
GLHVVNAGKLATGDLEGGLVSCTPAGAMVFVRRTYGDDLSGLSAVVIGRSNLFGKPMGAL
CEEEEECCCEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHE
LLAANATVTTAHSRTKNLPEVCRNADILVAAVGRPEMVKADWVKPGAMVIDVGINRVPAP
EEECCCEEEECCHHCCCCHHHHCCCCEEEEECCCCCEEECCCCCCCCEEEEECCCCCCCC
ERGEGKTKLVGDVAFKECAELASVITPVPGGVGPMTIAMLMANTIIAAHRKAGRRPPKF
CCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA