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The map label for this gene is fliF [H]

Identifier: 150395469

GI number: 150395469

Start: 264942

End: 266615

Strand: Direct

Name: fliF [H]

Synonym: Smed_0242

Alternate gene names: 150395469

Gene position: 264942-266615 (Clockwise)

Preceding gene: 150395468

Following gene: 150395470

Centisome position: 7.01

GC content: 62.31

Gene sequence:

>1674_bases
ATGAATCTGTTCGATCAATTCTCGACGTTTACCAAGAATCTGAGCAATCTCGGACAAGGGAAGCTCATTGCGCTGGCCGT
GGCCGGCATCGTGGCGATCGGCTTCGTCCTCGGGGCCGGCGTCTATGTCAATCGGCCGTCCTTCGAGACGCTATATGTCG
GGCTCGAACGCAGCGACGTCACCCAGATCAGCATTGCGCTTGCAGAGGCCAATGTCGATTTCGAAGTCGGTACCGACGGC
GGCAGCATCCAGGTGCCGGTGGGCATGACCGGCAAGGCTCGCCTGCTGCTTGCCGAACGCGGCTTGCCGAGCAGCGCGAA
TGCCGGATACGAACTCTTCGACAATGTCGGTTCGCTCGGGCTCACCTCTTTCATGCAGGAAGTCACGCGCGTCCGAGCAC
TCGAGGGGGAAATCGCGCGCACGATCCAGCAGATCTCCGGCATCGCCGCCGCGCGTGTCCACATCGTGATGCCGGAACGC
GGCAGCTTCCGCAAGGCCGAACAGACGCCGACCGCCTCCGTAATGATACGCGCAAGCGCCACGGTCGGTCGCAGCGCCGC
CGCTTCGATCCGCCATCTGGTCGCGTCCTCCGTGCCGGGCCTCGACGTTGATGACGTCACCGTGCTCGATTCGACCGGCC
AGCTTCTTGCATCCGGCGACGATCCTGCAAACAGCGCGCTCAACCAATCGCTTGGCGTCGTCCAGAACGTCCAGACCGAT
CTTGAAAAAAAGATCGACAATGCGCTCGCGCCGTTCCTCGGAATGGACAATTTCCGCACCAGCGTGACGGCCAGGCTCAA
CACGGATGCGCAGCAGATTCAGGAGACGGTTTTCGATCCGGAATCGCGGGTCGAGCGTTCGACGCGTGTGATCAAGGAAG
AGCAGAAGTCCAGCCAGCAGCAGCCGGATAATGCCGCAACTGTACAGCAGAACGTGCCCCAGGCGGCGCCAACCGGCGGA
GCCGGCCAACAGTCGAGCGACGAGGCGGCGAAAAAGGAAGAACAGACGAATTACGAGATCAACAGCAAGACGATCGCGAC
CGTCAAGAACAGCTATTCGATCGAGCGGCTTTCGATCGCGGTGGTCGTCAATCGCGGCCGCCTCGCTGCCATGGCCGGCG
AACCGGCGGACCAGGCTAAGATCGATGCCTATCTTGTGCAGATGCAGAAGATCGTTGCCTCCGCCGCCGGTATCGATCCT
GGTCGCGGCGATGTCGTTACGCTGAACGCAATGGACTTCGTGGAGACACAGCTGCTCGACCAGGCGGTGCCTGGCCCGGG
GATGATGGAAGTCCTGACACGGAACATCGGCGGCATCATCAACGCGCTTGCTTTCGTTGCGGTTGCCTTCCTGGTGGTAT
GGTTCGGGATGAGGCCGCTTGCGCGGCAGATGGGCTTCGGAGGTCAGTCGGGCCAGCTCGCAGGCGAAGCCGCCGGTCTG
GAACTGCCGGACTTCTCACCCGCCGCTGCCGGTGCAGGAGGAGCACTCATGGAGGGTTTTGGCTCCGACTTCGGCTTCGA
CGGTGGCGACGACCTTCTCAATCTCGGCGACGAGGCCGGTTTCAACCGCCGCGTCAAGGAAGGGCCGGAACGGCGACTTG
CCCGGATGGTGGAGATCAGCGAGGAGCGTGCGGCAAAGATCCTGCGAAAATGGGCACTCGACCGCGCCGCTTGA

Upstream 100 bases:

>100_bases
AGCGCAGGCCGTTTTCCAGCAAACCCGAATGTTGGATCTGCGCAGGGCATCGGCCGGGTAACCGGAGCGGGTGCCGTGCG
ACACTGGGTGCGGAAACAGA

Downstream 100 bases:

>100_bases
TAACCTCCGGCAGATCTGCATCAGAAGCAAGTCCCGCTCCGCAGGCGGGCCTTGCTTGTTTTTAACGCGCGCCCCCGTCC
AAGGCCGGTCAGGCGGACTT

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MNLFDQFSTFTKNLSNLGQGKLIALAVAGIVAIGFVLGAGVYVNRPSFETLYVGLERSDVTQISIALAEANVDFEVGTDG
GSIQVPVGMTGKARLLLAERGLPSSANAGYELFDNVGSLGLTSFMQEVTRVRALEGEIARTIQQISGIAAARVHIVMPER
GSFRKAEQTPTASVMIRASATVGRSAAASIRHLVASSVPGLDVDDVTVLDSTGQLLASGDDPANSALNQSLGVVQNVQTD
LEKKIDNALAPFLGMDNFRTSVTARLNTDAQQIQETVFDPESRVERSTRVIKEEQKSSQQQPDNAATVQQNVPQAAPTGG
AGQQSSDEAAKKEEQTNYEINSKTIATVKNSYSIERLSIAVVVNRGRLAAMAGEPADQAKIDAYLVQMQKIVASAAGIDP
GRGDVVTLNAMDFVETQLLDQAVPGPGMMEVLTRNIGGIINALAFVAVAFLVVWFGMRPLARQMGFGGQSGQLAGEAAGL
ELPDFSPAAAGAGGALMEGFGSDFGFDGGDDLLNLGDEAGFNRRVKEGPERRLARMVEISEERAAKILRKWALDRAA

Sequences:

>Translated_557_residues
MNLFDQFSTFTKNLSNLGQGKLIALAVAGIVAIGFVLGAGVYVNRPSFETLYVGLERSDVTQISIALAEANVDFEVGTDG
GSIQVPVGMTGKARLLLAERGLPSSANAGYELFDNVGSLGLTSFMQEVTRVRALEGEIARTIQQISGIAAARVHIVMPER
GSFRKAEQTPTASVMIRASATVGRSAAASIRHLVASSVPGLDVDDVTVLDSTGQLLASGDDPANSALNQSLGVVQNVQTD
LEKKIDNALAPFLGMDNFRTSVTARLNTDAQQIQETVFDPESRVERSTRVIKEEQKSSQQQPDNAATVQQNVPQAAPTGG
AGQQSSDEAAKKEEQTNYEINSKTIATVKNSYSIERLSIAVVVNRGRLAAMAGEPADQAKIDAYLVQMQKIVASAAGIDP
GRGDVVTLNAMDFVETQLLDQAVPGPGMMEVLTRNIGGIINALAFVAVAFLVVWFGMRPLARQMGFGGQSGQLAGEAAGL
ELPDFSPAAAGAGGALMEGFGSDFGFDGGDDLLNLGDEAGFNRRVKEGPERRLARMVEISEERAAKILRKWALDRAA
>Mature_557_residues
MNLFDQFSTFTKNLSNLGQGKLIALAVAGIVAIGFVLGAGVYVNRPSFETLYVGLERSDVTQISIALAEANVDFEVGTDG
GSIQVPVGMTGKARLLLAERGLPSSANAGYELFDNVGSLGLTSFMQEVTRVRALEGEIARTIQQISGIAAARVHIVMPER
GSFRKAEQTPTASVMIRASATVGRSAAASIRHLVASSVPGLDVDDVTVLDSTGQLLASGDDPANSALNQSLGVVQNVQTD
LEKKIDNALAPFLGMDNFRTSVTARLNTDAQQIQETVFDPESRVERSTRVIKEEQKSSQQQPDNAATVQQNVPQAAPTGG
AGQQSSDEAAKKEEQTNYEINSKTIATVKNSYSIERLSIAVVVNRGRLAAMAGEPADQAKIDAYLVQMQKIVASAAGIDP
GRGDVVTLNAMDFVETQLLDQAVPGPGMMEVLTRNIGGIINALAFVAVAFLVVWFGMRPLARQMGFGGQSGQLAGEAAGL
ELPDFSPAAAGAGGALMEGFGSDFGFDGGDDLLNLGDEAGFNRRVKEGPERRLARMVEISEERAAKILRKWALDRAA

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=420, Percent_Identity=28.5714285714286, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 58903; Mature: 58903

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFDQFSTFTKNLSNLGQGKLIALAVAGIVAIGFVLGAGVYVNRPSFETLYVGLERSDV
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCCCC
TQISIALAEANVDFEVGTDGGSIQVPVGMTGKARLLLAERGLPSSANAGYELFDNVGSLG
EEEEEEEEECCCCEEECCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHCCCHHH
LTSFMQEVTRVRALEGEIARTIQQISGIAAARVHIVMPERGSFRKAEQTPTASVMIRASA
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEEEEECCCCCCCCCCCCCCEEEEEEECC
TVGRSAAASIRHLVASSVPGLDVDDVTVLDSTGQLLASGDDPANSALNQSLGVVQNVQTD
CCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
LEKKIDNALAPFLGMDNFRTSVTARLNTDAQQIQETVFDPESRVERSTRVIKEEQKSSQQ
HHHHHHHHHHHHHCCCCHHHEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
QPDNAATVQQNVPQAAPTGGAGQQSSDEAAKKEEQTNYEINSKTIATVKNSYSIERLSIA
CCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCEECCCEEEEECCCCCEEEEEEE
VVVNRGRLAAMAGEPADQAKIDAYLVQMQKIVASAAGIDPGRGDVVTLNAMDFVETQLLD
EEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHH
QAVPGPGMMEVLTRNIGGIINALAFVAVAFLVVWFGMRPLARQMGFGGQSGQLAGEAAGL
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCC
ELPDFSPAAAGAGGALMEGFGSDFGFDGGDDLLNLGDEAGFNRRVKEGPERRLARMVEIS
CCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHH
EERAAKILRKWALDRAA
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLFDQFSTFTKNLSNLGQGKLIALAVAGIVAIGFVLGAGVYVNRPSFETLYVGLERSDV
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCCCC
TQISIALAEANVDFEVGTDGGSIQVPVGMTGKARLLLAERGLPSSANAGYELFDNVGSLG
EEEEEEEEECCCCEEECCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHCCCHHH
LTSFMQEVTRVRALEGEIARTIQQISGIAAARVHIVMPERGSFRKAEQTPTASVMIRASA
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEEEEECCCCCCCCCCCCCCEEEEEEECC
TVGRSAAASIRHLVASSVPGLDVDDVTVLDSTGQLLASGDDPANSALNQSLGVVQNVQTD
CCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
LEKKIDNALAPFLGMDNFRTSVTARLNTDAQQIQETVFDPESRVERSTRVIKEEQKSSQQ
HHHHHHHHHHHHHCCCCHHHEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
QPDNAATVQQNVPQAAPTGGAGQQSSDEAAKKEEQTNYEINSKTIATVKNSYSIERLSIA
CCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCEECCCEEEEECCCCCEEEEEEE
VVVNRGRLAAMAGEPADQAKIDAYLVQMQKIVASAAGIDPGRGDVVTLNAMDFVETQLLD
EEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHH
QAVPGPGMMEVLTRNIGGIINALAFVAVAFLVVWFGMRPLARQMGFGGQSGQLAGEAAGL
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCC
ELPDFSPAAAGAGGALMEGFGSDFGFDGGDDLLNLGDEAGFNRRVKEGPERRLARMVEIS
CCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHH
EERAAKILRKWALDRAA
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9858749; 11481430 [H]