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The map label for this gene is dinF [H]

Identifier: 150395387

GI number: 150395387

Start: 176103

End: 177473

Strand: Direct

Name: dinF [H]

Synonym: Smed_0160

Alternate gene names: 150395387

Gene position: 176103-177473 (Clockwise)

Preceding gene: 150395386

Following gene: 150395390

Centisome position: 4.66

GC content: 63.89

Gene sequence:

>1371_bases
ATGATGACGGCGACACAAAGCCTCGAGCGCGACTCCGATAGTCCCGAAGCCGGGCCATTTATCGTCACCAACAGGCTGAT
CTTCTCGATCGCCCTGCCGATGACGCTCGGCTTCCTTACGACGCCTCTTCTCGGTCTCGTCGACACGGCCGTCGTTGGCC
GGCTCGGCCGGGCGGAGTTGCTCGCGGGCCTCGCGGTCGGCGCGGTGATCTTCGACCTCATCTTTACCACCTTCAACTTC
CTTCGAGCCGCGACCACGGGCCTGGTCGCACAGGCTCTTGGACGCGGCGATCGACGCGAGCAGCAGGCGGTCTTCTGGCG
TTCGCTTGTGATTGCGCTCGTCACCGGCGCTGCCATCGTGCTTATATCTCCCTTTCTGCTTTCCGCCGGCCTCTGGCTCA
TGGGGCCCGGTCCGGACGTCGCGGAGGTGACGCGCACCTATTTCCAATATCGCATCCTCTCCGGCCCGGCCGCGCTCGCC
AACTACGCCATCCTCGGATTCGTGCTCGGGCGCGGCGAGGGCACTCTTGGCCTCATGCTGCAGACGCTGATAAACGGCAC
CAATATCGTGCTTTCGGTCCTGCTCGGCCTCGTTCTCGGCTGGGGTGTAGCCGGCGTCGCGATCGGGACCGTCGCCGGAG
AGGTTATCGGCGCGCTTGCGGGTTTCGCGATCGTCTATGGCCGCTTCGACAGCAAGGACGCGCCAGGTTGGGCGATGATC
CTTGCCGGCGACCGCCTGAAGAAATTGTTCGGTCTCAATCGCGACATCATGATCCGATCATTCGCCCTTCTGGCGGCCTT
CACGCTCGTGACCCGGATCGGCACCTCCTTCGGTCCCGTGACGCTTGCGGCGAACGCGGTGCTGATGACGATCTTCCTTG
TGGCGGGCTATTATCTCGATGGACTTGCCACCGCCGCCGAGCAGTTGACAGGCCGATCCATCGGCGCCGCTTATCGCCCC
GCTTTCGACCGGGTTCTGCGGATGACTGCGCTCTGGTCGCTCGGTCTCGCCGCCCTGACGACGCTCGCTCTGCTCGCCTT
CGGCAATGCCGTGGTGAATATGATGACCACGGCGCCGGATGTGCGCGCTCTTGCTTATGAATACATGCCATGGGCGGCGG
TGACGGCGCTGACCGGTGCTCTTGCCTTCCTCATGGACGGCGTCTTCATCGGAGCCGGCTGGTCGCGCGACATGCGCAAC
ATGATGCTGGCTGCCTTCATCGGATACGTCGCAGCGCTTGCCGTGCTCGTCCCTGCCTTCGGCAATCACGGCCTCTGGGC
CGCGCTCAACCTGTTCCTGCTCATGCGCGGCGTTTTCCTGCTGATAAGGATCCCGCGCCGGGCAGCTCAGACGTTCATGC
CAGTCCAGTAA

Upstream 100 bases:

>100_bases
GCAGAATTCCGCTTCCGGAAACCGCCCCTATTGGGCCATGGTGCTCTGCAGCTGAGTCGATTCCCGGATTTCCCGCGGAA
TCCGCGCGCAGGGAAAGAGG

Downstream 100 bases:

>100_bases
TCGACCCGGCTGTCGCGGATCTCCCGGATCGAGGCAAGCTTCTCGCGGTCGCAAAGCGCGGAGAGGCCGCGCACGATACG
GCCCGGCAAGCTCGGCCCCT

Product: MATE efflux family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MMTATQSLERDSDSPEAGPFIVTNRLIFSIALPMTLGFLTTPLLGLVDTAVVGRLGRAELLAGLAVGAVIFDLIFTTFNF
LRAATTGLVAQALGRGDRREQQAVFWRSLVIALVTGAAIVLISPFLLSAGLWLMGPGPDVAEVTRTYFQYRILSGPAALA
NYAILGFVLGRGEGTLGLMLQTLINGTNIVLSVLLGLVLGWGVAGVAIGTVAGEVIGALAGFAIVYGRFDSKDAPGWAMI
LAGDRLKKLFGLNRDIMIRSFALLAAFTLVTRIGTSFGPVTLAANAVLMTIFLVAGYYLDGLATAAEQLTGRSIGAAYRP
AFDRVLRMTALWSLGLAALTTLALLAFGNAVVNMMTTAPDVRALAYEYMPWAAVTALTGALAFLMDGVFIGAGWSRDMRN
MMLAAFIGYVAALAVLVPAFGNHGLWAALNLFLLMRGVFLLIRIPRRAAQTFMPVQ

Sequences:

>Translated_456_residues
MMTATQSLERDSDSPEAGPFIVTNRLIFSIALPMTLGFLTTPLLGLVDTAVVGRLGRAELLAGLAVGAVIFDLIFTTFNF
LRAATTGLVAQALGRGDRREQQAVFWRSLVIALVTGAAIVLISPFLLSAGLWLMGPGPDVAEVTRTYFQYRILSGPAALA
NYAILGFVLGRGEGTLGLMLQTLINGTNIVLSVLLGLVLGWGVAGVAIGTVAGEVIGALAGFAIVYGRFDSKDAPGWAMI
LAGDRLKKLFGLNRDIMIRSFALLAAFTLVTRIGTSFGPVTLAANAVLMTIFLVAGYYLDGLATAAEQLTGRSIGAAYRP
AFDRVLRMTALWSLGLAALTTLALLAFGNAVVNMMTTAPDVRALAYEYMPWAAVTALTGALAFLMDGVFIGAGWSRDMRN
MMLAAFIGYVAALAVLVPAFGNHGLWAALNLFLLMRGVFLLIRIPRRAAQTFMPVQ
>Mature_456_residues
MMTATQSLERDSDSPEAGPFIVTNRLIFSIALPMTLGFLTTPLLGLVDTAVVGRLGRAELLAGLAVGAVIFDLIFTTFNF
LRAATTGLVAQALGRGDRREQQAVFWRSLVIALVTGAAIVLISPFLLSAGLWLMGPGPDVAEVTRTYFQYRILSGPAALA
NYAILGFVLGRGEGTLGLMLQTLINGTNIVLSVLLGLVLGWGVAGVAIGTVAGEVIGALAGFAIVYGRFDSKDAPGWAMI
LAGDRLKKLFGLNRDIMIRSFALLAAFTLVTRIGTSFGPVTLAANAVLMTIFLVAGYYLDGLATAAEQLTGRSIGAAYRP
AFDRVLRMTALWSLGLAALTTLALLAFGNAVVNMMTTAPDVRALAYEYMPWAAVTALTGALAFLMDGVFIGAGWSRDMRN
MMLAAFIGYVAALAVLVPAFGNHGLWAALNLFLLMRGVFLLIRIPRRAAQTFMPVQ

Specific function: Unknown

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790477, Length=420, Percent_Identity=36.1904761904762, Blast_Score=180, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 48322; Mature: 48322

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
>Mature Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503 [H]