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The map label for this gene is rbsB [H]

Identifier: 150395330

GI number: 150395330

Start: 113078

End: 114103

Strand: Direct

Name: rbsB [H]

Synonym: Smed_0102

Alternate gene names: 150395330

Gene position: 113078-114103 (Clockwise)

Preceding gene: 150395327

Following gene: 150395331

Centisome position: 2.99

GC content: 62.87

Gene sequence:

>1026_bases
ATGAAGAAGACTGTTCTTTCTGCCGCATTCGGCGCGCTTGCACTCGGCGTGGCCTTCGCATCGCCTTCGCAGGCGGCCGA
CGTGTCGGCCTGCCTCATCACCAAAACCGACGCCAATCCTTTCTTTGCGAAAATGAAGGAAGGCGCGACCGCCAAGGCCA
AGGAACTGGGCGTGGCCCTGAAGTCCTATGCCGGTAAGATCGATGGAGATTCCGAGAGCCAGGTTGCCGCGATCGAGACA
TGCATCGCCGACGGTGCGAAAGGTATTCTGATCGCCGCCTCCGACACCCAGGGCATCGTGCCTCACGTCAAGAAGGCGCG
GGACGCCGGTCTCCTGGTCATCGCACTCGATACGCCGCTCGAGCCGCTCGACTCCGCCGACGCGACCTTTGCAACGGACA
ACCTGCTCGCCGGCAAGCTGATCGGGCAATGGGCTGCCGCAACGCTCGGCGACGCCGCCAAGGACGCCAAGGTGGCATTC
CTCGACCTTACGCCGTCTCAGCCTTCCGTCGACGTGCTGCGCGACCAGGGCTTCATGATCGGCTTCGGCATCGACCCCAA
GGACCCGAACAAGATCGGCGACGAGGATGATCCGCGCATCGTCGGCCATGACATCACCAACGGCAACGAAGAAGGCGGCC
GGTCTGCAATGGAGAACCTCCTCCAGAAAGATCCGACCATCAATGTCGTCCACACGATCAACGAACCGGCGGCCGCCGGC
GCCTACGAGGCGCTGAAGGCTCTCGGCCGCGAGCAGGACGTGCTGATCGTTTCCGTCGATGGCGGTTGCCCGGGGGTCAA
GAACGTCGCCGAGGGTGCAATCGGAGCGACGTCGCAGCAATACCCGCTGATGATGGCGGCGCTCGGCATCGAGGCAATCA
AGAAGTTCGCTGACACCGGCGAAAAGCCGGTGCCGACAGAGGGCAAGGATTTCGTGGACACGGGAGTCTCGCTCGTCACC
GACAAGCCGGTTGATGGTCTGGAATCGATCGACACCAAGACCGGCCTGGAGAAGTGCTGGGGCTGA

Upstream 100 bases:

>100_bases
TTTGATGTTTGATTGGCTCTTGGAAGCGTCGTCGGCAGCTGAGGAGAATGCTGCGGGCATGAAGTCGGCGCTCCGGCATT
GTCATCCTTGGGAGGATCTA

Downstream 100 bases:

>100_bases
CCGGCCCGTTCCGGATTGGATGCGAACGGCGGCTCGTCCGCCCTTCGCACAAGCGCCGAGTTCTTTGACAAGGCACGAAA
CCTGTGCAGTTTCATCAGGG

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKKTVLSAAFGALALGVAFASPSQAADVSACLITKTDANPFFAKMKEGATAKAKELGVALKSYAGKIDGDSESQVAAIET
CIADGAKGILIAASDTQGIVPHVKKARDAGLLVIALDTPLEPLDSADATFATDNLLAGKLIGQWAAATLGDAAKDAKVAF
LDLTPSQPSVDVLRDQGFMIGFGIDPKDPNKIGDEDDPRIVGHDITNGNEEGGRSAMENLLQKDPTINVVHTINEPAAAG
AYEALKALGREQDVLIVSVDGGCPGVKNVAEGAIGATSQQYPLMMAALGIEAIKKFADTGEKPVPTEGKDFVDTGVSLVT
DKPVDGLESIDTKTGLEKCWG

Sequences:

>Translated_341_residues
MKKTVLSAAFGALALGVAFASPSQAADVSACLITKTDANPFFAKMKEGATAKAKELGVALKSYAGKIDGDSESQVAAIET
CIADGAKGILIAASDTQGIVPHVKKARDAGLLVIALDTPLEPLDSADATFATDNLLAGKLIGQWAAATLGDAAKDAKVAF
LDLTPSQPSVDVLRDQGFMIGFGIDPKDPNKIGDEDDPRIVGHDITNGNEEGGRSAMENLLQKDPTINVVHTINEPAAAG
AYEALKALGREQDVLIVSVDGGCPGVKNVAEGAIGATSQQYPLMMAALGIEAIKKFADTGEKPVPTEGKDFVDTGVSLVT
DKPVDGLESIDTKTGLEKCWG
>Mature_341_residues
MKKTVLSAAFGALALGVAFASPSQAADVSACLITKTDANPFFAKMKEGATAKAKELGVALKSYAGKIDGDSESQVAAIET
CIADGAKGILIAASDTQGIVPHVKKARDAGLLVIALDTPLEPLDSADATFATDNLLAGKLIGQWAAATLGDAAKDAKVAF
LDLTPSQPSVDVLRDQGFMIGFGIDPKDPNKIGDEDDPRIVGHDITNGNEEGGRSAMENLLQKDPTINVVHTINEPAAAG
AYEALKALGREQDVLIVSVDGGCPGVKNVAEGAIGATSQQYPLMMAALGIEAIKKFADTGEKPVPTEGKDFVDTGVSLVT
DKPVDGLESIDTKTGLEKCWG

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=307, Percent_Identity=29.6416938110749, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790526, Length=278, Percent_Identity=26.6187050359712, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790674, Length=262, Percent_Identity=27.0992366412214, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35160; Mature: 35160

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTVLSAAFGALALGVAFASPSQAADVSACLITKTDANPFFAKMKEGATAKAKELGVAL
CCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHH
KSYAGKIDGDSESQVAAIETCIADGAKGILIAASDTQGIVPHVKKARDAGLLVIALDTPL
HHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEEECCCC
EPLDSADATFATDNLLAGKLIGQWAAATLGDAAKDAKVAFLDLTPSQPSVDVLRDQGFMI
CCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHCCCEEE
GFGIDPKDPNKIGDEDDPRIVGHDITNGNEEGGRSAMENLLQKDPTINVVHTINEPAAAG
EECCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCC
AYEALKALGREQDVLIVSVDGGCPGVKNVAEGAIGATSQQYPLMMAALGIEAIKKFADTG
HHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
EKPVPTEGKDFVDTGVSLVTDKPVDGLESIDTKTGLEKCWG
CCCCCCCCCHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKTVLSAAFGALALGVAFASPSQAADVSACLITKTDANPFFAKMKEGATAKAKELGVAL
CCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHH
KSYAGKIDGDSESQVAAIETCIADGAKGILIAASDTQGIVPHVKKARDAGLLVIALDTPL
HHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEEECCCC
EPLDSADATFATDNLLAGKLIGQWAAATLGDAAKDAKVAFLDLTPSQPSVDVLRDQGFMI
CCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHCCCEEE
GFGIDPKDPNKIGDEDDPRIVGHDITNGNEEGGRSAMENLLQKDPTINVVHTINEPAAAG
EECCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCC
AYEALKALGREQDVLIVSVDGGCPGVKNVAEGAIGATSQQYPLMMAALGIEAIKKFADTG
HHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
EKPVPTEGKDFVDTGVSLVTDKPVDGLESIDTKTGLEKCWG
CCCCCCCCCHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]