Click here to switch to the map view.

The map label for this gene is prpB [C]

Identifier: 150395302

GI number: 150395302

Start: 82168

End: 82944

Strand: Direct

Name: prpB [C]

Synonym: Smed_0074

Alternate gene names: 150395302

Gene position: 82168-82944 (Clockwise)

Preceding gene: 150395301

Following gene: 150395304

Centisome position: 2.17

GC content: 64.86

Gene sequence:

>777_bases
ATGAGCCAGGAAGAAAATGCGCTTGCCTTCGAGGCACTGCACCGGAAAGGCGATCCGGTGATTCTGTACAACATCTGGGA
TGCGGGCAGCGCCCGCTGCGTGGCAGAGGCCGGCGCCAAGGCGCTTGCCACCGGGAGCTGGTCGGTCGCTGCGGCACACG
GCTATACGGACGGGCAGAAGCTGCCGCTGCCTCTGCTGCTCGAGACCGTGCGCGAGATCGTCGCGGCAACCGGCCTCCCG
CTCTCGGTGGATTTCGAGGGTGCCTATTCGGAGGATCCGGCTCAGGCTGCGGCGAACGTCGCGCAAATTATGGATGCGGG
GTGCATCGGGATCAATTTCGAGGACCAGGTGATAGGAAAGGGCGGTGTCCATCCGATCGACGGGCAGGCGGCTCGCGTCC
GCGCAATCCGCGAAATGGCCGAGCGACGGGGCGTTCGGTTGTTTATCAACGCTCGCACCGACCTCTTCCTGCAAGAGGGT
GATGCGGAGCGCCACACGGCACTGATGGAGGACGCGATTGCCCGCGCCGAAGCCTATGCCGATGCGGGCGCGAGTGGTTT
CTTCGCGCCGGGGCTCGCCGATGAGACCCTCATCGCCAGGCTTTGCGCGGCTTCGCCTTTGCCTGTCAACGTGATGATGA
AGCCGGGAGCGCCGGATATCGCCAGGCTTTCCGCTGCCGGCGTGGGGAGAGTGAGCTATGGACCGTTCCCTTATCGGGGC
ATGATGGCTTGGCTGCGCGGCGAGGCCGAGAAAGTCTATGCGAGCGCGGGGAAGTAG

Upstream 100 bases:

>100_bases
GCGGAGCTGTTCAAAGGTTGGGACGGGGAGGAGCCGGTGCAGGCCGGCGCTCTTTCGGTTTGACAGGATCGATGAACGCA
TTGGATTGGGAGAAGACACG

Downstream 100 bases:

>100_bases
CGAAGGGCGGGATAGGGTGCGCTGATGTACAAGAGATTCGCTCCTCTCCTCGGGTTGAACCCGAGGAGAGGAGCGAAATG
ACGCGAATTAAGGCGCGCCT

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSQEENALAFEALHRKGDPVILYNIWDAGSARCVAEAGAKALATGSWSVAAAHGYTDGQKLPLPLLLETVREIVAATGLP
LSVDFEGAYSEDPAQAAANVAQIMDAGCIGINFEDQVIGKGGVHPIDGQAARVRAIREMAERRGVRLFINARTDLFLQEG
DAERHTALMEDAIARAEAYADAGASGFFAPGLADETLIARLCAASPLPVNVMMKPGAPDIARLSAAGVGRVSYGPFPYRG
MMAWLRGEAEKVYASAGK

Sequences:

>Translated_258_residues
MSQEENALAFEALHRKGDPVILYNIWDAGSARCVAEAGAKALATGSWSVAAAHGYTDGQKLPLPLLLETVREIVAATGLP
LSVDFEGAYSEDPAQAAANVAQIMDAGCIGINFEDQVIGKGGVHPIDGQAARVRAIREMAERRGVRLFINARTDLFLQEG
DAERHTALMEDAIARAEAYADAGASGFFAPGLADETLIARLCAASPLPVNVMMKPGAPDIARLSAAGVGRVSYGPFPYRG
MMAWLRGEAEKVYASAGK
>Mature_257_residues
SQEENALAFEALHRKGDPVILYNIWDAGSARCVAEAGAKALATGSWSVAAAHGYTDGQKLPLPLLLETVREIVAATGLPL
SVDFEGAYSEDPAQAAANVAQIMDAGCIGINFEDQVIGKGGVHPIDGQAARVRAIREMAERRGVRLFINARTDLFLQEGD
AERHTALMEDAIARAEAYADAGASGFFAPGLADETLIARLCAASPLPVNVMMKPGAPDIARLSAAGVGRVSYGPFPYRGM
MAWLRGEAEKVYASAGK

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 27201; Mature: 27070

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQEENALAFEALHRKGDPVILYNIWDAGSARCVAEAGAKALATGSWSVAAAHGYTDGQK
CCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCEEECCCCEEEEECCCCCCCC
LPLPLLLETVREIVAATGLPLSVDFEGAYSEDPAQAAANVAQIMDAGCIGINFEDQVIGK
CCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCEEECCCCHHCCCC
GGVHPIDGQAARVRAIREMAERRGVRLFINARTDLFLQEGDAERHTALMEDAIARAEAYA
CCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHH
DAGASGFFAPGLADETLIARLCAASPLPVNVMMKPGAPDIARLSAAGVGRVSYGPFPYRG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCHHH
MMAWLRGEAEKVYASAGK
HHHHHHCCHHHHHHCCCC
>Mature Secondary Structure 
SQEENALAFEALHRKGDPVILYNIWDAGSARCVAEAGAKALATGSWSVAAAHGYTDGQK
CCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCEEECCCCEEEEECCCCCCCC
LPLPLLLETVREIVAATGLPLSVDFEGAYSEDPAQAAANVAQIMDAGCIGINFEDQVIGK
CCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCEEECCCCHHCCCC
GGVHPIDGQAARVRAIREMAERRGVRLFINARTDLFLQEGDAERHTALMEDAIARAEAYA
CCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHH
DAGASGFFAPGLADETLIARLCAASPLPVNVMMKPGAPDIARLSAAGVGRVSYGPFPYRG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCHHH
MMAWLRGEAEKVYASAGK
HHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]