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The map label for this gene is grpE [H]

Identifier: 150395240

GI number: 150395240

Start: 10909

End: 11535

Strand: Direct

Name: grpE [H]

Synonym: Smed_0012

Alternate gene names: 150395240

Gene position: 10909-11535 (Clockwise)

Preceding gene: 150395239

Following gene: 150395247

Centisome position: 0.29

GC content: 63.16

Gene sequence:

>627_bases
ATGACCGACGAAACGAACAAGAACGGACCCGAGGCCGCGGCACCTGAAGAATTCGATGCAGCCGCGCAGCCGGAAACCGC
GGAAGAGCCGGAGGCGGACGTTTCCGCCGCCGCGCCGGATCCTCTTGAGCTCGCAAAGGCGGAGAATGCGGAGTTGCGTG
ACAAATACCTGCGCCTTGCCGCAGAAATGGACAATCTTCGCCGGCGCACCGAACGTGACGTGAAGGACGCGAAGTCCTAT
TCAGTCGCGGGCTTCGCGCGCGATATGTTGGCCGTTTCGGATAACCTTCGCCGTGCGCTTGACGCTATTCCGGCGGATGC
CCGAGAGGCGGGCGATGCCGGCTTGAAGGCACTCATCGAGGGTGTCGAGATGACGGAGCGGTCGATGCTTGCCGCGCTTG
AGCGTCATGGCGTGAAGCAGCTGGACCCCACGGGCCAGAAGTTCGACCCGAACTTCCATCAGGCCATGTTCGAAGTTCCG
AACCCCGAGGTTCCGAACAACACGGTCGTGCAGGTCGTCCAGGCCGGATACACCATTGGCGAACGCGTTCTGCGCCCGGC
AATGGTCGGCGTCGCCAAGGGCGGTCCAAAGGCGGCTTCCTCCGAAGGCGAAACGCCCCCTGCCTAA

Upstream 100 bases:

>100_bases
ATCGGGCTTCAAATCCAGGCAACAGAACGTTCAAGCAAAGCGACTTCCGCTGGTTTGATGATGCCGAAGACAGCAAAAGA
CAGAAATTTCGGAGAACGTC

Downstream 100 bases:

>100_bases
CGGCGGGAAGAAGTCTTCAAGAATGCGAGGCTCGCCGCGTCGGCGCCCTCCTTCGGCTGGTCAGTCCTCGGGTCAAGCTC
GGCGCTAAACCCGAGGAGAG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTDETNKNGPEAAAPEEFDAAAQPETAEEPEADVSAAAPDPLELAKAENAELRDKYLRLAAEMDNLRRRTERDVKDAKSY
SVAGFARDMLAVSDNLRRALDAIPADAREAGDAGLKALIEGVEMTERSMLAALERHGVKQLDPTGQKFDPNFHQAMFEVP
NPEVPNNTVVQVVQAGYTIGERVLRPAMVGVAKGGPKAASSEGETPPA

Sequences:

>Translated_208_residues
MTDETNKNGPEAAAPEEFDAAAQPETAEEPEADVSAAAPDPLELAKAENAELRDKYLRLAAEMDNLRRRTERDVKDAKSY
SVAGFARDMLAVSDNLRRALDAIPADAREAGDAGLKALIEGVEMTERSMLAALERHGVKQLDPTGQKFDPNFHQAMFEVP
NPEVPNNTVVQVVQAGYTIGERVLRPAMVGVAKGGPKAASSEGETPPA
>Mature_207_residues
TDETNKNGPEAAAPEEFDAAAQPETAEEPEADVSAAAPDPLELAKAENAELRDKYLRLAAEMDNLRRRTERDVKDAKSYS
VAGFARDMLAVSDNLRRALDAIPADAREAGDAGLKALIEGVEMTERSMLAALERHGVKQLDPTGQKFDPNFHQAMFEVPN
PEVPNNTVVQVVQAGYTIGERVLRPAMVGVAKGGPKAASSEGETPPA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=140, Percent_Identity=39.2857142857143, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI40255109, Length=147, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=7e-18,
Organism=Escherichia coli, GI1788967, Length=197, Percent_Identity=38.5786802030457, Blast_Score=114, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17552458, Length=149, Percent_Identity=40.2684563758389, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6324806, Length=151, Percent_Identity=40.3973509933775, Blast_Score=122, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24653432, Length=180, Percent_Identity=44.4444444444444, Blast_Score=151, Evalue=3e-37,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22294; Mature: 22163

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDETNKNGPEAAAPEEFDAAAQPETAEEPEADVSAAAPDPLELAKAENAELRDKYLRLA
CCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHH
AEMDNLRRRTERDVKDAKSYSVAGFARDMLAVSDNLRRALDAIPADAREAGDAGLKALIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GVEMTERSMLAALERHGVKQLDPTGQKFDPNFHQAMFEVPNPEVPNNTVVQVVQAGYTIG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
ERVLRPAMVGVAKGGPKAASSEGETPPA
HHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDETNKNGPEAAAPEEFDAAAQPETAEEPEADVSAAAPDPLELAKAENAELRDKYLRLA
CCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHH
AEMDNLRRRTERDVKDAKSYSVAGFARDMLAVSDNLRRALDAIPADAREAGDAGLKALIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GVEMTERSMLAALERHGVKQLDPTGQKFDPNFHQAMFEVPNPEVPNNTVVQVVQAGYTIG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
ERVLRPAMVGVAKGGPKAASSEGETPPA
HHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]