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The map label for this gene is dnaA [H]

Identifier: 150395230

GI number: 150395230

Start: 1405

End: 2850

Strand: Direct

Name: dnaA [H]

Synonym: Smed_0002

Alternate gene names: 150395230

Gene position: 1405-2850 (Clockwise)

Preceding gene: 150395229

Following gene: 150395231

Centisome position: 0.04

GC content: 61.62

Gene sequence:

>1446_bases
ATGCGGCATGACGCACTCTTCGAGCGGGTAAGTGCGCGTTTGAAAGCCCAGGTCGGACCGGATGTCTTCGCGAGTTGGTT
TGGGCGCCTCAAGCTTCATTCAGTGTCGAAAAGCGTCGTGCGCCTCTCGGTCCCGACGACCTTTCTGAAGTCCTGGATCA
ATAATCGTTATCTCGATCTGATCACCTCGCTCGTCCAACAGGAGGACAGCGAGATATTGAAGGTGGAGATCCTGGTGCGC
ACCGCCACCCGCGGACATCGTCCGGCGGCTCCTGAAGAGACGGTTTCTGCCGCGGTGGAGACGGTGCCCGTCGCTTCTGT
TCGCCGTTCTGCGACATCCCCTTCGGTGGCGATCTCTGCCTCGACCGTTGCGGCAGCTCCGGCGAGGCCCCATCAGACGC
CGCTTTTCGGGTCGCCGCTTGATCAGCGGTACGGTTTCGATAGTTTCGTAGAGGGTTCCTCCAATCGGGTCGCGCTCGCG
GCAGCGCGTACGATTGCCGAGGCCGGCGCCGGCGCCGTGCGCTTCAATCCTCTTTTCATTCATTCGAGCGTTGGCCTCGG
TAAGACCCATCTGCTGCAGGCGATCGCGCTGGCGGCGCTCCAGAGTGCCCGGGCGCCGCGCGTCGTTTATCTGACGGCCG
AATATTTCATGTGGCGTTTCGCAACGGCAATCCGCGACAATGATGCGCTCTCGTTGAAGGAGTCGCTTCGCAACATCGAC
CTTCTCATCATCGACGATATGCAGTTTCTACAGGGCAAGTCGATCCAGCACGAGTTCTGCCATCTCCTGAACATGCTGCT
CGACTCCGCCAAACAGGTGGTCGTCGCCGCGGATCGCGCGCCGTGGGAATTGGAATCGCTCGACAGCCGCGTTCGCTCGC
GCCTTCAGGGAGGTGTCGCGATCGAGATGGAAGGGCCGGACTACGAAATGCGCCTGGAAATGCTGAAGCGCCGCCTCGAA
GCGGCGCGCCAGGACGATGCGACACTCGAAATTCCGCTCGAGATCCTGAGCCACGTCGCCCGCAACGTAACGGCCAGCGG
CCGCGAGCTGGAGGGAGCTTTCAATCAGCTTCTCTTCCGCCGCTCCTTCGAGCCGCAGCTTTCGATCGAGCGGGTCGATG
AACTGCTGGGCCATCTCGTCAATGCCGGCGAACCGCGCCGGGTCCGCATCGAGGACATCCAGCGCGTCGTGGCCAAGCAT
TACAACGTCTCGCGCCAGGAACTGGTTTCGAACCGCCGCACCCGCGTGATCGTCAAGCCGCGCCAGATAGCCATGTATCT
GTCCAAGACGCTGACTCCGCGTTCCTTTCCGGAGATCGGCCGCCGCTTCGGCGGACGCGACCATACGACCGTGCTCCACG
CGGTGCGCAAGATCGAAGAGCTGATTTCGGGCGACACCAAGCTCAGCCATGAGATCGAGCTGTTGAAGCGCCTGATCAAC
GAATAG

Upstream 100 bases:

>100_bases
GAAATTGGAAGGCGGCAAAATGCAGATGAATTTGGCGACGGCACCTGAAGGTTTTCAGGCCGGAAGCAATCAGTCTCAGG
CGGCGGGGGAAAAACACGAC

Downstream 100 bases:

>100_bases
AGACAGGTAAGTTTCCACTTACAAAAGCCCGGTCCGCCGGGCTTTTTTGTTGAATCGGAGCAGTTCCGGAAAACCGGCAC
CGTTTCCGCTCGGAGGTGCA

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MRHDALFERVSARLKAQVGPDVFASWFGRLKLHSVSKSVVRLSVPTTFLKSWINNRYLDLITSLVQQEDSEILKVEILVR
TATRGHRPAAPEETVSAAVETVPVASVRRSATSPSVAISASTVAAAPARPHQTPLFGSPLDQRYGFDSFVEGSSNRVALA
AARTIAEAGAGAVRFNPLFIHSSVGLGKTHLLQAIALAALQSARAPRVVYLTAEYFMWRFATAIRDNDALSLKESLRNID
LLIIDDMQFLQGKSIQHEFCHLLNMLLDSAKQVVVAADRAPWELESLDSRVRSRLQGGVAIEMEGPDYEMRLEMLKRRLE
AARQDDATLEIPLEILSHVARNVTASGRELEGAFNQLLFRRSFEPQLSIERVDELLGHLVNAGEPRRVRIEDIQRVVAKH
YNVSRQELVSNRRTRVIVKPRQIAMYLSKTLTPRSFPEIGRRFGGRDHTTVLHAVRKIEELISGDTKLSHEIELLKRLIN
E

Sequences:

>Translated_481_residues
MRHDALFERVSARLKAQVGPDVFASWFGRLKLHSVSKSVVRLSVPTTFLKSWINNRYLDLITSLVQQEDSEILKVEILVR
TATRGHRPAAPEETVSAAVETVPVASVRRSATSPSVAISASTVAAAPARPHQTPLFGSPLDQRYGFDSFVEGSSNRVALA
AARTIAEAGAGAVRFNPLFIHSSVGLGKTHLLQAIALAALQSARAPRVVYLTAEYFMWRFATAIRDNDALSLKESLRNID
LLIIDDMQFLQGKSIQHEFCHLLNMLLDSAKQVVVAADRAPWELESLDSRVRSRLQGGVAIEMEGPDYEMRLEMLKRRLE
AARQDDATLEIPLEILSHVARNVTASGRELEGAFNQLLFRRSFEPQLSIERVDELLGHLVNAGEPRRVRIEDIQRVVAKH
YNVSRQELVSNRRTRVIVKPRQIAMYLSKTLTPRSFPEIGRRFGGRDHTTVLHAVRKIEELISGDTKLSHEIELLKRLIN
E
>Mature_481_residues
MRHDALFERVSARLKAQVGPDVFASWFGRLKLHSVSKSVVRLSVPTTFLKSWINNRYLDLITSLVQQEDSEILKVEILVR
TATRGHRPAAPEETVSAAVETVPVASVRRSATSPSVAISASTVAAAPARPHQTPLFGSPLDQRYGFDSFVEGSSNRVALA
AARTIAEAGAGAVRFNPLFIHSSVGLGKTHLLQAIALAALQSARAPRVVYLTAEYFMWRFATAIRDNDALSLKESLRNID
LLIIDDMQFLQGKSIQHEFCHLLNMLLDSAKQVVVAADRAPWELESLDSRVRSRLQGGVAIEMEGPDYEMRLEMLKRRLE
AARQDDATLEIPLEILSHVARNVTASGRELEGAFNQLLFRRSFEPQLSIERVDELLGHLVNAGEPRRVRIEDIQRVVAKH
YNVSRQELVSNRRTRVIVKPRQIAMYLSKTLTPRSFPEIGRRFGGRDHTTVLHAVRKIEELISGDTKLSHEIELLKRLIN
E

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=463, Percent_Identity=34.7732181425486, Blast_Score=269, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 53860; Mature: 53860

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS01008 DNAA ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHDALFERVSARLKAQVGPDVFASWFGRLKLHSVSKSVVRLSVPTTFLKSWINNRYLDL
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCHHHHH
ITSLVQQEDSEILKVEILVRTATRGHRPAAPEETVSAAVETVPVASVRRSATSPSVAISA
HHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEC
STVAAAPARPHQTPLFGSPLDQRYGFDSFVEGSSNRVALAAARTIAEAGAGAVRFNPLFI
HHHCCCCCCCCCCCCCCCCHHHHCCCHHHHCCCCCEEEHHHHHHHHHCCCCEEEECEEEE
HSSVGLGKTHLLQAIALAALQSARAPRVVYLTAEYFMWRFATAIRDNDALSLKESLRNID
ECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHCCCC
LLIIDDMQFLQGKSIQHEFCHLLNMLLDSAKQVVVAADRAPWELESLDSRVRSRLQGGVA
EEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCEE
IEMEGPDYEMRLEMLKRRLEAARQDDATLEIPLEILSHVARNVTASGRELEGAFNQLLFR
EEECCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RSFEPQLSIERVDELLGHLVNAGEPRRVRIEDIQRVVAKHYNVSRQELVSNRRTRVIVKP
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCHHHHHCCCCCEEEECH
RQIAMYLSKTLTPRSFPEIGRRFGGRDHTTVLHAVRKIEELISGDTKLSHEIELLKRLIN
HHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
E
C
>Mature Secondary Structure
MRHDALFERVSARLKAQVGPDVFASWFGRLKLHSVSKSVVRLSVPTTFLKSWINNRYLDL
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCHHHHH
ITSLVQQEDSEILKVEILVRTATRGHRPAAPEETVSAAVETVPVASVRRSATSPSVAISA
HHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEC
STVAAAPARPHQTPLFGSPLDQRYGFDSFVEGSSNRVALAAARTIAEAGAGAVRFNPLFI
HHHCCCCCCCCCCCCCCCCHHHHCCCHHHHCCCCCEEEHHHHHHHHHCCCCEEEECEEEE
HSSVGLGKTHLLQAIALAALQSARAPRVVYLTAEYFMWRFATAIRDNDALSLKESLRNID
ECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHCCCC
LLIIDDMQFLQGKSIQHEFCHLLNMLLDSAKQVVVAADRAPWELESLDSRVRSRLQGGVA
EEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCEE
IEMEGPDYEMRLEMLKRRLEAARQDDATLEIPLEILSHVARNVTASGRELEGAFNQLLFR
EEECCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RSFEPQLSIERVDELLGHLVNAGEPRRVRIEDIQRVVAKHYNVSRQELVSNRRTRVIVKP
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCHHHHHCCCCCEEEECH
RQIAMYLSKTLTPRSFPEIGRRFGGRDHTTVLHAVRKIEELISGDTKLSHEIELLKRLIN
HHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]