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The map label for this gene is amtB [C]

Identifier: 150401904

GI number: 150401904

Start: 1541622

End: 1542833

Strand: Direct

Name: amtB [C]

Synonym: Maeo_1482

Alternate gene names: 150401904

Gene position: 1541622-1542833 (Clockwise)

Preceding gene: 150401903

Following gene: 150401911

Centisome position: 98.22

GC content: 38.04

Gene sequence:

>1212_bases
ATGGTTGTAGATATAAACACAGTGCAAGAGGGATTAAATGCATTAGCTACCTCAGGAGATGTATTTTATCTTGTAGTTAT
GGGGGTTCTTGTATTTTTAATGCAATTAGGATTTGCAATGCTTGAAGGAGGGCAAGTTAGGAAGAAAAATGTCAACAATG
TTATGATGAAGAATTACAGTGATTGGATTATTGGATGTCTTTCATGGTTATTTGTAGGTAGTGTTTTGGCAACTTCAATA
GTGCCTGCTGATTTCATTAGCTGGTGGGGAAAAATATTCATGACTTCATGGGATTCAATAAACATTGATTTAGCAAACTG
GTTCTTTGGTTTAGTATTTGCGGCAACAGCGGCAACAATAGTTTCAGGAGGGGTTGCCGAAAGAATTAAATTCTCTGCAT
ATGTATTAATTGCTGTATTAATTGTAGCATTTGTATATCCATTCTTTGTATATTTGGGTCCTTGGGGCTCTGGTATGATA
GGTTGGGCAGATTATGCTGGAAGTTTAATTGTGCATGGATTGGGTGGATTTTTGGCATTGGGGTCAATCATGGCATTAGG
TCCAAGAATAGGAAGATTTGTAAATGGTCAAGCTATTCCATTATTGGGACATAACATACCAATGGCAGTATTTGGTTCAT
TTGCTTTGGCAATCGGTTGGTATGGATTTAATGTTGGGAGCTCCCTTGCATTAGGTGATATATCAGGTTTAGTAGTAGCT
ACAACCACACTTGCTATGGCAGGTGGTGGATTAGGTGGTATGTTAGCATCTAAAAATGATGTGTTATTTACGGCAAACGG
TATAGTAGCAGGGCTTGTAGCAATTTGTGCAGGAACTGACATTGTTAGTCCAATAGCAGCATTAATAATAGGTCTTTTAG
CAGGAATTCAAGTTCCAATTGTATATAAATTAATTGAAAAAGCAAAATTGGACGATGTATGTGGTGTTGTTCCAGTGCAT
GGAACAGCAGGAATATTGGGAGCTCTGTTGGCAGGAATATTTGGAATGGAAGCTCTTGGTGGTGCAGGTGGAGTTAGCTT
TGTAGACCAAGCAATAGCTTGTATATTCACAATGGTTTATGGAACAGGGCTAGGATTTGGAATTGCTAAATTAGCTGGTT
TATTGACTGGCGGATTAAGAGTAAAAGAAGAAGAAGAAAAACTTGGTCTTGATTTGGCAGAGCACAAATTACCAGCTTAT
CCAGAAGAATAA

Upstream 100 bases:

>100_bases
TAGAACTGGAGAACGGGATAAAACTGCAATTTAATTAAAAAATAAACTTAAAAAATAAACTTATGAATAAATAATATAAT
GTATCAGTATAGGTGAAAAT

Downstream 100 bases:

>100_bases
AATAAATATTATTTAATTTTTTTATTATTTTATCTCCTTAATTTTTAATTTTATTTTTTATACTGTTTTTTTTAAATTGT
TTGCCATATATTTTATAATA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MVVDINTVQEGLNALATSGDVFYLVVMGVLVFLMQLGFAMLEGGQVRKKNVNNVMMKNYSDWIIGCLSWLFVGSVLATSI
VPADFISWWGKIFMTSWDSINIDLANWFFGLVFAATAATIVSGGVAERIKFSAYVLIAVLIVAFVYPFFVYLGPWGSGMI
GWADYAGSLIVHGLGGFLALGSIMALGPRIGRFVNGQAIPLLGHNIPMAVFGSFALAIGWYGFNVGSSLALGDISGLVVA
TTTLAMAGGGLGGMLASKNDVLFTANGIVAGLVAICAGTDIVSPIAALIIGLLAGIQVPIVYKLIEKAKLDDVCGVVPVH
GTAGILGALLAGIFGMEALGGAGGVSFVDQAIACIFTMVYGTGLGFGIAKLAGLLTGGLRVKEEEEKLGLDLAEHKLPAY
PEE

Sequences:

>Translated_403_residues
MVVDINTVQEGLNALATSGDVFYLVVMGVLVFLMQLGFAMLEGGQVRKKNVNNVMMKNYSDWIIGCLSWLFVGSVLATSI
VPADFISWWGKIFMTSWDSINIDLANWFFGLVFAATAATIVSGGVAERIKFSAYVLIAVLIVAFVYPFFVYLGPWGSGMI
GWADYAGSLIVHGLGGFLALGSIMALGPRIGRFVNGQAIPLLGHNIPMAVFGSFALAIGWYGFNVGSSLALGDISGLVVA
TTTLAMAGGGLGGMLASKNDVLFTANGIVAGLVAICAGTDIVSPIAALIIGLLAGIQVPIVYKLIEKAKLDDVCGVVPVH
GTAGILGALLAGIFGMEALGGAGGVSFVDQAIACIFTMVYGTGLGFGIAKLAGLLTGGLRVKEEEEKLGLDLAEHKLPAY
PEE
>Mature_403_residues
MVVDINTVQEGLNALATSGDVFYLVVMGVLVFLMQLGFAMLEGGQVRKKNVNNVMMKNYSDWIIGCLSWLFVGSVLATSI
VPADFISWWGKIFMTSWDSINIDLANWFFGLVFAATAATIVSGGVAERIKFSAYVLIAVLIVAFVYPFFVYLGPWGSGMI
GWADYAGSLIVHGLGGFLALGSIMALGPRIGRFVNGQAIPLLGHNIPMAVFGSFALAIGWYGFNVGSSLALGDISGLVVA
TTTLAMAGGGLGGMLASKNDVLFTANGIVAGLVAICAGTDIVSPIAALIIGLLAGIQVPIVYKLIEKAKLDDVCGVVPVH
GTAGILGALLAGIFGMEALGGAGGVSFVDQAIACIFTMVYGTGLGFGIAKLAGLLTGGLRVKEEEEKLGLDLAEHKLPAY
PEE

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=435, Percent_Identity=30.3448275862069, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17550182, Length=432, Percent_Identity=32.8703703703704, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17550180, Length=455, Percent_Identity=31.6483516483516, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI32564042, Length=415, Percent_Identity=30.1204819277108, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI133931100, Length=433, Percent_Identity=28.175519630485, Blast_Score=108, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6325396, Length=409, Percent_Identity=26.4058679706601, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321559, Length=407, Percent_Identity=25.5528255528256, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6324187, Length=436, Percent_Identity=25.2293577981651, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI161078314, Length=437, Percent_Identity=31.350114416476, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 41887; Mature: 41887

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVDINTVQEGLNALATSGDVFYLVVMGVLVFLMQLGFAMLEGGQVRKKNVNNVMMKNYS
CEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHCCH
DWIIGCLSWLFVGSVLATSIVPADFISWWGKIFMTSWDSINIDLANWFFGLVFAATAATI
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHH
VSGGVAERIKFSAYVLIAVLIVAFVYPFFVYLGPWGSGMIGWADYAGSLIVHGLGGFLAL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GSIMALGPRIGRFVNGQAIPLLGHNIPMAVFGSFALAIGWYGFNVGSSLALGDISGLVVA
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHH
TTTLAMAGGGLGGMLASKNDVLFTANGIVAGLVAICAGTDIVSPIAALIIGLLAGIQVPI
HHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHH
VYKLIEKAKLDDVCGVVPVHGTAGILGALLAGIFGMEALGGAGGVSFVDQAIACIFTMVY
HHHHHHHHCHHHHCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GTGLGFGIAKLAGLLTGGLRVKEEEEKLGLDLAEHKLPAYPEE
HCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCHHHCCCCCCCCC
>Mature Secondary Structure
MVVDINTVQEGLNALATSGDVFYLVVMGVLVFLMQLGFAMLEGGQVRKKNVNNVMMKNYS
CEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHCCH
DWIIGCLSWLFVGSVLATSIVPADFISWWGKIFMTSWDSINIDLANWFFGLVFAATAATI
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHH
VSGGVAERIKFSAYVLIAVLIVAFVYPFFVYLGPWGSGMIGWADYAGSLIVHGLGGFLAL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GSIMALGPRIGRFVNGQAIPLLGHNIPMAVFGSFALAIGWYGFNVGSSLALGDISGLVVA
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHH
TTTLAMAGGGLGGMLASKNDVLFTANGIVAGLVAICAGTDIVSPIAALIIGLLAGIQVPI
HHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHH
VYKLIEKAKLDDVCGVVPVHGTAGILGALLAGIFGMEALGGAGGVSFVDQAIACIFTMVY
HHHHHHHHCHHHHCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GTGLGFGIAKLAGLLTGGLRVKEEEEKLGLDLAEHKLPAYPEE
HCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]