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The map label for this gene is ilvG [C]

Identifier: 150401870

GI number: 150401870

Start: 1505400

End: 1506923

Strand: Direct

Name: ilvG [C]

Synonym: Maeo_1448

Alternate gene names: 150401870

Gene position: 1505400-1506923 (Clockwise)

Preceding gene: 150401869

Following gene: 150401871

Centisome position: 95.92

GC content: 27.82

Gene sequence:

>1524_bases
ATGAACTTTACAGACGAGATATTAGATTTTTTAATAAAAAAGAATATAAAAACAGTTTTTTCATATCCTGGGGAACAAAT
TCTTCCATTATACAACGGTTTAAATGAATCACCCATAAAAAATATAATGGTAAGAAGCGAACAGGGAGCTTCACATATGG
CGGACGGATACAGCAGAATTACAAATCATGTTGGAGTATGTTTAGCAACTGCGGGACCTGGTGCAACAAATTTAATGACT
GGAATAGCTACGGCATATAAAGATTCCTCCTCTGTGATAGCAATTACAGGGAGATGTAAAAAAGAATATATTAATAAAAA
CTATTTTCAAGAAATAAATATGGATTTTTTAAATTTGCAAAATGGATATTTTGTAGATGCCCCGGATATAAAATATTTTG
AAAAGGCATTTAATGAATCAATAAATACCAAAAAGCCCATTCACTTAAATATCCCGTATGGCGTATTATTGGATAGCAAT
GACGACAATAACCGTAATAATAATGAAAATAACTATAAAGATGAAAAAAATAATAAAAAAGACAATACATCTATATTAAA
TAACATAGATTATAACACCACAGGTAAACCATTGTTGTTGATTGGCCAGGGTATTTATGGGAATTTATCTTATAATGACA
TATTAAAAATAAATGACATATTAAAAAATAGCCACATTCCAATAGCCACAACATATCCTGCAAGGGGAGTTATTGATGAA
AATAACCCAATCTGTCTTGGTTTAGTTGGGGGAAGAGGCACAGAAAAAGCAAACCAATATATTTTAAATTGTGATACTGT
GTATTGCTTAGGAGCCTCTTTATCATACAATACATTACCTCCCCTAATTAGAGATAAAATAATACCTAAAATAAAAGATA
TACCAATAAATATTAATAATTTAGAGGATATTGAATTATTAATAAATACATTAAACTACAAATATAATAATGTATATATT
AATAATATCGGCAATAATATTAATCCCAATAATAAATTATGTGGAGATTATTCTTTAAAAATTAATGAAATATTAAATAA
TTTGCCAGATGATACCATAATTACGACAGATGCAGGAAATCATACAGTTTTTGTATCATTATTAAAAACTTGTGGAGCTC
CGAAAAATATTATTTCTTCCCACAGCATGGGGACTATGGGATTTGGGCTTCCTGCATCAATTGGAGTTAAATTTGGATGT
TTGGATAATAATATTGATAGGGAAGTAGTTTCGATAAATGGAGACGGCGGGTTTCAAATGAATATACAAGAATTAGCTAC
CGTTTCCGAAAACAACTTAAAAATATTAATAATTATAATGAAAAACAACAGATTAAACATATTTGGAGATATTAAAAATC
CAGATTTCAATAAAATAGCAGATGGATATAATATAGATAATATATATATCTCAGATATTGATGAAATAGAAAATAACATA
AAATATTATTTAAATAACAACAAACCTTATTTAATGGTAGTTGAATGTGAAAATGAGTCATTGCCAAAAAAATCATTTAA
TTAA

Upstream 100 bases:

>100_bases
AATAAAGAAAGTAATAGTATTAACAATAATAAAAAAGACAATGATAACAACATTCAACAAACAACATTAACAAATATATT
TAATGGGATTAAGGGATAAA

Downstream 100 bases:

>100_bases
TAACTAATAATTACAAAATAACTAAATAACAAATAATACGGATTAATGGAGTGAAAATATATGAATAAATTATTTAAGAT
TTTAACTGGGAATATACACT

Product: thiamine pyrophosphate binding domain-containing protein

Products: 2-acetolactate; CO2

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MNFTDEILDFLIKKNIKTVFSYPGEQILPLYNGLNESPIKNIMVRSEQGASHMADGYSRITNHVGVCLATAGPGATNLMT
GIATAYKDSSSVIAITGRCKKEYINKNYFQEINMDFLNLQNGYFVDAPDIKYFEKAFNESINTKKPIHLNIPYGVLLDSN
DDNNRNNNENNYKDEKNNKKDNTSILNNIDYNTTGKPLLLIGQGIYGNLSYNDILKINDILKNSHIPIATTYPARGVIDE
NNPICLGLVGGRGTEKANQYILNCDTVYCLGASLSYNTLPPLIRDKIIPKIKDIPININNLEDIELLINTLNYKYNNVYI
NNIGNNINPNNKLCGDYSLKINEILNNLPDDTIITTDAGNHTVFVSLLKTCGAPKNIISSHSMGTMGFGLPASIGVKFGC
LDNNIDREVVSINGDGGFQMNIQELATVSENNLKILIIIMKNNRLNIFGDIKNPDFNKIADGYNIDNIYISDIDEIENNI
KYYLNNNKPYLMVVECENESLPKKSFN

Sequences:

>Translated_507_residues
MNFTDEILDFLIKKNIKTVFSYPGEQILPLYNGLNESPIKNIMVRSEQGASHMADGYSRITNHVGVCLATAGPGATNLMT
GIATAYKDSSSVIAITGRCKKEYINKNYFQEINMDFLNLQNGYFVDAPDIKYFEKAFNESINTKKPIHLNIPYGVLLDSN
DDNNRNNNENNYKDEKNNKKDNTSILNNIDYNTTGKPLLLIGQGIYGNLSYNDILKINDILKNSHIPIATTYPARGVIDE
NNPICLGLVGGRGTEKANQYILNCDTVYCLGASLSYNTLPPLIRDKIIPKIKDIPININNLEDIELLINTLNYKYNNVYI
NNIGNNINPNNKLCGDYSLKINEILNNLPDDTIITTDAGNHTVFVSLLKTCGAPKNIISSHSMGTMGFGLPASIGVKFGC
LDNNIDREVVSINGDGGFQMNIQELATVSENNLKILIIIMKNNRLNIFGDIKNPDFNKIADGYNIDNIYISDIDEIENNI
KYYLNNNKPYLMVVECENESLPKKSFN
>Mature_507_residues
MNFTDEILDFLIKKNIKTVFSYPGEQILPLYNGLNESPIKNIMVRSEQGASHMADGYSRITNHVGVCLATAGPGATNLMT
GIATAYKDSSSVIAITGRCKKEYINKNYFQEINMDFLNLQNGYFVDAPDIKYFEKAFNESINTKKPIHLNIPYGVLLDSN
DDNNRNNNENNYKDEKNNKKDNTSILNNIDYNTTGKPLLLIGQGIYGNLSYNDILKINDILKNSHIPIATTYPARGVIDE
NNPICLGLVGGRGTEKANQYILNCDTVYCLGASLSYNTLPPLIRDKIIPKIKDIPININNLEDIELLINTLNYKYNNVYI
NNIGNNINPNNKLCGDYSLKINEILNNLPDDTIITTDAGNHTVFVSLLKTCGAPKNIISSHSMGTMGFGLPASIGVKFGC
LDNNIDREVVSINGDGGFQMNIQELATVSENNLKILIIIMKNNRLNIFGDIKNPDFNKIADGYNIDNIYISDIDEIENNI
KYYLNNNKPYLMVVECENESLPKKSFN

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=490, Percent_Identity=22.2448979591837, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1790104, Length=546, Percent_Identity=26.5567765567766, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87081685, Length=550, Percent_Identity=26.3636363636364, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1786717, Length=532, Percent_Identity=21.2406015037594, Blast_Score=113, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=560, Percent_Identity=24.1071428571429, Blast_Score=179, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6320816, Length=540, Percent_Identity=24.4444444444444, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6

Molecular weight: Translated: 56639; Mature: 56639

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFTDEILDFLIKKNIKTVFSYPGEQILPLYNGLNESPIKNIMVRSEQGASHMADGYSRI
CCHHHHHHHHHHHHCCHHHHCCCCCEEHHHHCCCCCCCHHHHEEECCCCCHHHHHHHHHH
TNHVGVCLATAGPGATNLMTGIATAYKDSSSVIAITGRCKKEYINKNYFQEINMDFLNLQ
HHCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCHHHHHCCCEEEECC
NGYFVDAPDIKYFEKAFNESINTKKPIHLNIPYGVLLDSNDDNNRNNNENNYKDEKNNKK
CCEEECCCCHHHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCC
DNTSILNNIDYNTTGKPLLLIGQGIYGNLSYNDILKINDILKNSHIPIATTYPARGVIDE
CCHHHHHCCCCCCCCCEEEEEECCEECCCCCCCEEEEHHHHCCCCCCEEEECCCCCEEEC
NNPICLGLVGGRGTEKANQYILNCDTVYCLGASLSYNTLPPLIRDKIIPKIKDIPININN
CCCEEEEEECCCCCCCCCEEEEECCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCEECCC
LEDIELLINTLNYKYNNVYINNIGNNINPNNKLCGDYSLKINEILNNLPDDTIITTDAGN
CHHHHHHHHHHCEEEEEEEEECCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCEEEECCCC
HTVFVSLLKTCGAPKNIISSHSMGTMGFGLPASIGVKFGCLDNNIDREVVSINGDGGFQM
CEEHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHCEEEEECCCCCCCEEEEEECCCCEEE
NIQELATVSENNLKILIIIMKNNRLNIFGDIKNPDFNKIADGYNIDNIYISDIDEIENNI
EHHHHHHCCCCCCEEEEEEEECCEEEEEECCCCCCHHHHCCCCCCCEEEECCHHHHCCCE
KYYLNNNKPYLMVVECENESLPKKSFN
EEEEECCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNFTDEILDFLIKKNIKTVFSYPGEQILPLYNGLNESPIKNIMVRSEQGASHMADGYSRI
CCHHHHHHHHHHHHCCHHHHCCCCCEEHHHHCCCCCCCHHHHEEECCCCCHHHHHHHHHH
TNHVGVCLATAGPGATNLMTGIATAYKDSSSVIAITGRCKKEYINKNYFQEINMDFLNLQ
HHCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCHHHHHCCCEEEECC
NGYFVDAPDIKYFEKAFNESINTKKPIHLNIPYGVLLDSNDDNNRNNNENNYKDEKNNKK
CCEEECCCCHHHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCC
DNTSILNNIDYNTTGKPLLLIGQGIYGNLSYNDILKINDILKNSHIPIATTYPARGVIDE
CCHHHHHCCCCCCCCCEEEEEECCEECCCCCCCEEEEHHHHCCCCCCEEEECCCCCEEEC
NNPICLGLVGGRGTEKANQYILNCDTVYCLGASLSYNTLPPLIRDKIIPKIKDIPININN
CCCEEEEEECCCCCCCCCEEEEECCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCEECCC
LEDIELLINTLNYKYNNVYINNIGNNINPNNKLCGDYSLKINEILNNLPDDTIITTDAGN
CHHHHHHHHHHCEEEEEEEEECCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCEEEECCCC
HTVFVSLLKTCGAPKNIISSHSMGTMGFGLPASIGVKFGCLDNNIDREVVSINGDGGFQM
CEEHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHCEEEEECCCCCCCEEEEEECCCCEEE
NIQELATVSENNLKILIIIMKNNRLNIFGDIKNPDFNKIADGYNIDNIYISDIDEIENNI
EHHHHHHCCCCCCEEEEEEEECCEEEEEECCCCCCHHHHCCCCCCCEEEECCHHHHCCCE
KYYLNNNKPYLMVVECENESLPKKSFN
EEEEECCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]

Substrates: pyruvate

Specific reaction: 2 pyruvate = 2-acetolactate + CO2

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]