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The map label for this gene is gltX

Identifier: 150401533

GI number: 150401533

Start: 1155121

End: 1156806

Strand: Direct

Name: gltX

Synonym: Maeo_1110

Alternate gene names: 150401533

Gene position: 1155121-1156806 (Clockwise)

Preceding gene: 150401532

Following gene: 150401534

Centisome position: 73.6

GC content: 32.15

Gene sequence:

>1686_bases
TTGAGCGACATACGAGACACAATTTTAAAATATGTTCTTCAAAATTCAATAAAATTTAAAGGAGCTCCCAACCCAAAGGC
AATCATGGGAAAAATATTGGGAGAAAATCCAGATTTAAGAAAAAAGGCAGGGGAAGTTTCAAAAATAATTGCAGAAATAA
TAAAAGAAATAGAAAATATGCCACTAAATGAACAACAGGCAAAATTAGAATCCCTTGCCCCAGAATTATTAAACAGCAAA
CCAGCAGAAAGAAAAAAGAAAGAGCTACAATTAAAAAATGTAAAAGAGGGAGCTCCTGTTGTAATGAGATTTGCACCAAA
TCCATCTGGACCACTTCATTTGGGACATGCCAGAGCTGCTGTATTAAATGATTATTTTGCAAAAAAATATAATGGAAAAT
TAATTTTAAGGTTGGAGGACACAGACCCAAAAAGAGTAATGCCTGAAGCTTATGAAATGATTAAAGAGGATTTAGAATGG
CTTGGAGTAAAAATAGATGATGTAATTGTTCAATCCGATAGATTACCCATTTATTACGAATATGGAACAAAATTAATAGA
AATGGGGCATGCCTATGTTTGTGATTGTGAGGCAGAAGAATTCAGGGAGCTCCGAGCTAATGGAAAACCATGTGTATGTA
GGGATTTAGGAGTGGAGAAAAATTTAGAACTATGGAATAAAATGCTAAATAATGAAATAGATGGAGTTGCAGTCAGGTTA
AAAACAGACATAGAGCATAAAAACCCATCAATAAGAGATTTTCCTATATTTAGAATTGAAAACACACCCCACCCAAAAAC
AGGCACAAAATACAGAGTTTATCCACTAATGAACCTGTCCGTATCTGTTGATGACCATCTTTTAGGTATGACCCATGTAT
TGAGGGGAAAAGACCATATAGTAAATACGGAAAAACAAAAATACATATTTGATTACTTTGGCTGGGAAATGCCCGAATAT
ATACATTATGGTATTTTAAAAATAGATGGACCGATTTTAAGCACATCAAAAATGAGCGAAGGAATTAAAAACAATGAATT
CACAGGTTGGGACGACCCTAGATTTGGAACGCTAAGGGCATTGAAAAGAAGAGGAATAAAACCAGAGGCAATTTATAAAT
CAATGGTAGATATTGGAGTAAAACAGGCAGATGTAAAATACTCATGGGAAAACTTACATGCTGTAAATAAAGATATAATT
GATAAAGATGCTCGACGGTTCTTCTTTGTAGAAAATCCAAAAACTTTAATAATCGAGGGAGCAAAAATCACTACGATAAA
TTTAAGAGCTCACCCAAGTAGGGAAGAATTTGGAACAAGAGAATTAATATTTGATGGAAATATTTATATTTCCGATGATT
TGGAAGTAAATAAAATGTATAGATTGATGGAGCTCCATAATATTGTAGTTGAAAAAATAGAAAATAACACAATATATGCC
AAATACCACTCCGATGACTTCAAAATAGCAAAAGAAAATAAAGCAAAAATAATACATTGGATTCCTGTAAAAGATGCCAT
TCCTACTACCGTAATAAATACAGAGGGAGAAAAAATTAAAGGATTTGCTGAAAAAGACTTTAAAATAGTAAATATTGATG
ATTTTGTGCAATTTGAAAGATTTGGATTTGTAAGAATAGATGAAAAGAAAGATGATGAAATAATTTGTTATTATACCCAT
AATTAA

Upstream 100 bases:

>100_bases
TTTTTACGATTTTACGATTATTTTAATTATTAATTATATATATTATTTAATCATACTTTTTTAACATAAAAAAACTTTTT
AAAAATTATCAGGTGAAAAA

Downstream 100 bases:

>100_bases
TTTTTATTATGGGAGCTCCTTTTATGACTTTTTTTATATATTGTTATACAATATTTTATTAATAATATTTTATTACGATA
TTATCTATTTTAGGGGGATA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MSDIRDTILKYVLQNSIKFKGAPNPKAIMGKILGENPDLRKKAGEVSKIIAEIIKEIENMPLNEQQAKLESLAPELLNSK
PAERKKKELQLKNVKEGAPVVMRFAPNPSGPLHLGHARAAVLNDYFAKKYNGKLILRLEDTDPKRVMPEAYEMIKEDLEW
LGVKIDDVIVQSDRLPIYYEYGTKLIEMGHAYVCDCEAEEFRELRANGKPCVCRDLGVEKNLELWNKMLNNEIDGVAVRL
KTDIEHKNPSIRDFPIFRIENTPHPKTGTKYRVYPLMNLSVSVDDHLLGMTHVLRGKDHIVNTEKQKYIFDYFGWEMPEY
IHYGILKIDGPILSTSKMSEGIKNNEFTGWDDPRFGTLRALKRRGIKPEAIYKSMVDIGVKQADVKYSWENLHAVNKDII
DKDARRFFFVENPKTLIIEGAKITTINLRAHPSREEFGTRELIFDGNIYISDDLEVNKMYRLMELHNIVVEKIENNTIYA
KYHSDDFKIAKENKAKIIHWIPVKDAIPTTVINTEGEKIKGFAEKDFKIVNIDDFVQFERFGFVRIDEKKDDEIICYYTH
N

Sequences:

>Translated_561_residues
MSDIRDTILKYVLQNSIKFKGAPNPKAIMGKILGENPDLRKKAGEVSKIIAEIIKEIENMPLNEQQAKLESLAPELLNSK
PAERKKKELQLKNVKEGAPVVMRFAPNPSGPLHLGHARAAVLNDYFAKKYNGKLILRLEDTDPKRVMPEAYEMIKEDLEW
LGVKIDDVIVQSDRLPIYYEYGTKLIEMGHAYVCDCEAEEFRELRANGKPCVCRDLGVEKNLELWNKMLNNEIDGVAVRL
KTDIEHKNPSIRDFPIFRIENTPHPKTGTKYRVYPLMNLSVSVDDHLLGMTHVLRGKDHIVNTEKQKYIFDYFGWEMPEY
IHYGILKIDGPILSTSKMSEGIKNNEFTGWDDPRFGTLRALKRRGIKPEAIYKSMVDIGVKQADVKYSWENLHAVNKDII
DKDARRFFFVENPKTLIIEGAKITTINLRAHPSREEFGTRELIFDGNIYISDDLEVNKMYRLMELHNIVVEKIENNTIYA
KYHSDDFKIAKENKAKIIHWIPVKDAIPTTVINTEGEKIKGFAEKDFKIVNIDDFVQFERFGFVRIDEKKDDEIICYYTH
N
>Mature_560_residues
SDIRDTILKYVLQNSIKFKGAPNPKAIMGKILGENPDLRKKAGEVSKIIAEIIKEIENMPLNEQQAKLESLAPELLNSKP
AERKKKELQLKNVKEGAPVVMRFAPNPSGPLHLGHARAAVLNDYFAKKYNGKLILRLEDTDPKRVMPEAYEMIKEDLEWL
GVKIDDVIVQSDRLPIYYEYGTKLIEMGHAYVCDCEAEEFRELRANGKPCVCRDLGVEKNLELWNKMLNNEIDGVAVRLK
TDIEHKNPSIRDFPIFRIENTPHPKTGTKYRVYPLMNLSVSVDDHLLGMTHVLRGKDHIVNTEKQKYIFDYFGWEMPEYI
HYGILKIDGPILSTSKMSEGIKNNEFTGWDDPRFGTLRALKRRGIKPEAIYKSMVDIGVKQADVKYSWENLHAVNKDIID
KDARRFFFVENPKTLIIEGAKITTINLRAHPSREEFGTRELIFDGNIYISDDLEVNKMYRLMELHNIVVEKIENNTIYAK
YHSDDFKIAKENKAKIIHWIPVKDAIPTTVINTEGEKIKGFAEKDFKIVNIDDFVQFERFGFVRIDEKKDDEIICYYTHN

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI62241042, Length=430, Percent_Identity=30.2325581395349, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI4826960, Length=357, Percent_Identity=29.4117647058824, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI134288884, Length=286, Percent_Identity=32.8671328671329, Blast_Score=138, Evalue=2e-32,
Organism=Escherichia coli, GI1786895, Length=506, Percent_Identity=30.4347826086957, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1788743, Length=311, Percent_Identity=28.9389067524116, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI226510925, Length=297, Percent_Identity=30.6397306397306, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17511007, Length=353, Percent_Identity=32.0113314447592, Blast_Score=202, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17543332, Length=335, Percent_Identity=29.8507462686567, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71993394, Length=312, Percent_Identity=27.5641025641026, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321192, Length=508, Percent_Identity=30.3149606299213, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6324742, Length=444, Percent_Identity=29.954954954955, Blast_Score=213, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6324540, Length=301, Percent_Identity=32.5581395348837, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24649466, Length=535, Percent_Identity=29.9065420560748, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI17864488, Length=512, Percent_Identity=27.1484375, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24665321, Length=303, Percent_Identity=30.3630363036304, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_META3 (A6UW15)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001325299.1
- ProteinModelPortal:   A6UW15
- SMR:   A6UW15
- STRING:   A6UW15
- GeneID:   5326562
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_1110
- eggNOG:   arNOG04636
- HOGENOM:   HBG334108
- OMA:   MRFAPNP
- ProtClustDB:   PRK04156
- BioCyc:   MAEO419665:MAEO_1110-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_A
- InterPro:   IPR001412
- InterPro:   IPR004526
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR022888
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:2.40.240.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00463

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.17

Molecular weight: Translated: 64864; Mature: 64732

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIRDTILKYVLQNSIKFKGAPNPKAIMGKILGENPDLRKKAGEVSKIIAEIIKEIENM
CCHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
PLNEQQAKLESLAPELLNSKPAERKKKELQLKNVKEGAPVVMRFAPNPSGPLHLGHARAA
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEECHHHHH
VLNDYFAKKYNGKLILRLEDTDPKRVMPEAYEMIKEDLEWLGVKIDDVIVQSDRLPIYYE
HHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEEHEEEECCCCCEEEC
YGTKLIEMGHAYVCDCEAEEFRELRANGKPCVCRDLGVEKNLELWNKMLNNEIDGVAVRL
CCCHHHHCCCEEEECCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCCEEEEE
KTDIEHKNPSIRDFPIFRIENTPHPKTGTKYRVYPLMNLSVSVDDHLLGMTHVLRGKDHI
EECCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEEEECCHHHHHHHHHHCCCCCC
VNTEKQKYIFDYFGWEMPEYIHYGILKIDGPILSTSKMSEGIKNNEFTGWDDPRFGTLRA
CCCCCHHEEHHHHCCCCCHHHEEEEEEECCCEECHHHHHCCCCCCCCCCCCCCCHHHHHH
LKRRGIKPEAIYKSMVDIGVKQADVKYSWENLHAVNKDIIDKDARRFFFVENPKTLIIEG
HHHCCCCHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEC
AKITTINLRAHPSREEFGTRELIFDGNIYISDDLEVNKMYRLMELHNIVVEKIENNTIYA
CEEEEEEEEECCCHHHCCCEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEE
KYHSDDFKIAKENKAKIIHWIPVKDAIPTTVINTEGEKIKGFAEKDFKIVNIDDFVQFER
EEECCCCEEECCCCCEEEEEEECCCCCCCEEECCCCHHCCCCCCCCEEEEEHHHHHHHHH
FGFVRIDEKKDDEIICYYTHN
CCEEEECCCCCCCEEEEEECC
>Mature Secondary Structure 
SDIRDTILKYVLQNSIKFKGAPNPKAIMGKILGENPDLRKKAGEVSKIIAEIIKEIENM
CHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
PLNEQQAKLESLAPELLNSKPAERKKKELQLKNVKEGAPVVMRFAPNPSGPLHLGHARAA
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEECHHHHH
VLNDYFAKKYNGKLILRLEDTDPKRVMPEAYEMIKEDLEWLGVKIDDVIVQSDRLPIYYE
HHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEEHEEEECCCCCEEEC
YGTKLIEMGHAYVCDCEAEEFRELRANGKPCVCRDLGVEKNLELWNKMLNNEIDGVAVRL
CCCHHHHCCCEEEECCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCCEEEEE
KTDIEHKNPSIRDFPIFRIENTPHPKTGTKYRVYPLMNLSVSVDDHLLGMTHVLRGKDHI
EECCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEEEECCHHHHHHHHHHCCCCCC
VNTEKQKYIFDYFGWEMPEYIHYGILKIDGPILSTSKMSEGIKNNEFTGWDDPRFGTLRA
CCCCCHHEEHHHHCCCCCHHHEEEEEEECCCEECHHHHHCCCCCCCCCCCCCCCHHHHHH
LKRRGIKPEAIYKSMVDIGVKQADVKYSWENLHAVNKDIIDKDARRFFFVENPKTLIIEG
HHHCCCCHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEC
AKITTINLRAHPSREEFGTRELIFDGNIYISDDLEVNKMYRLMELHNIVVEKIENNTIYA
CEEEEEEEEECCCHHHCCCEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEE
KYHSDDFKIAKENKAKIIHWIPVKDAIPTTVINTEGEKIKGFAEKDFKIVNIDDFVQFER
EEECCCCEEECCCCCEEEEEEECCCCCCCEEECCCCHHCCCCCCCCEEEEEHHHHHHHHH
FGFVRIDEKKDDEIICYYTHN
CCEEEECCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA