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The map label for this gene is frhB [H]

Identifier: 150401466

GI number: 150401466

Start: 1075033

End: 1075881

Strand: Reverse

Name: frhB [H]

Synonym: Maeo_1042

Alternate gene names: 150401466

Gene position: 1075881-1075033 (Counterclockwise)

Preceding gene: 150401467

Following gene: 150401465

Centisome position: 68.55

GC content: 37.1

Gene sequence:

>849_bases
ATGGATGCTTTTGGAAATTATAAATCAGTAATATCTGCAAGAATTGCAGATAAAGCAGTGCTTAAAAAAGTACAGGATGG
AGGTATTGTAACCGCAGCATTTGTATATGGATTGGAAAACAACCTATTGGATGGTGCAGTTGTGGCAAGTGTGGGGGAAG
AATTTAGGGCCGTTCCTAAGGTGGCAACCACAGTTGAAGAAATATTGGAAGCCGCAGGAACAAAATATACCACCTGTCCA
AATTTATCGGTATTAAAACAAGCAATTAGAGAATACGGCTGTGAAAAGGTTGGAGTTGTTGGAACGCCATGCCAAATAAT
AGCTACAAGAAAATCCTTAAAATATCCAGTTGGATTTAGGCATATGAACGATAAAATTGCCTTAACAGTTGGTATTTTCT
GTATGGAAAACTTCCCATACAATGGAATGAAAACCATAGTAGAAGAACACTGCGGAGTAAAAATGGATGATGTGGCTAAA
TTAGACATTGGAAAAGGAAAATTCTGGACATACACAAAATGGGGGGAAACAAAGTCAATTAAATTGGCCGATACTCACCC
ATATGAGCAAATAGCATGTCATGTATGCACAGATTATACCGCAGAATTAGCCGACATTTCAACAGGTTCAGTGGGTAGCC
CTGATGGTTGGAGTACAGTATTTGCAAGAACTGAAAAAGGAGAAGAATTCCTTAATGGCATGGTAGAATCAGGATATTTA
GAAACCAAACCAGTAAATGAAGGTAAATTTGGTTTAGAATTATTGGCAAAACTTGCAAACATTAAAAAGTCCAAAAACCA
AAAAGAAATAGACCATAGAAAAGAAATTGGACTTCCAGTTCCTTACTAA

Upstream 100 bases:

>100_bases
GGTGCTGTGGATTCCAATGTCCAAGAATAGAACTTCCTAAAAATGTCATGGTATTGGAATAATTCATTGGATAATGGTAG
AGCATAATCGAGGTGATTAA

Downstream 100 bases:

>100_bases
TTATTTATTTTTTAACTTTTTTAATTTATTATTTTGGAGATATTTCGTGGAGCTCCCAATATAAATTATAAGTATTTTTA
AATATAATAAAATATTTACT

Product: coenzyme F420-reducing hydrogenase subunit beta

Products: NA

Alternate protein names: 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta; FRH [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MDAFGNYKSVISARIADKAVLKKVQDGGIVTAAFVYGLENNLLDGAVVASVGEEFRAVPKVATTVEEILEAAGTKYTTCP
NLSVLKQAIREYGCEKVGVVGTPCQIIATRKSLKYPVGFRHMNDKIALTVGIFCMENFPYNGMKTIVEEHCGVKMDDVAK
LDIGKGKFWTYTKWGETKSIKLADTHPYEQIACHVCTDYTAELADISTGSVGSPDGWSTVFARTEKGEEFLNGMVESGYL
ETKPVNEGKFGLELLAKLANIKKSKNQKEIDHRKEIGLPVPY

Sequences:

>Translated_282_residues
MDAFGNYKSVISARIADKAVLKKVQDGGIVTAAFVYGLENNLLDGAVVASVGEEFRAVPKVATTVEEILEAAGTKYTTCP
NLSVLKQAIREYGCEKVGVVGTPCQIIATRKSLKYPVGFRHMNDKIALTVGIFCMENFPYNGMKTIVEEHCGVKMDDVAK
LDIGKGKFWTYTKWGETKSIKLADTHPYEQIACHVCTDYTAELADISTGSVGSPDGWSTVFARTEKGEEFLNGMVESGYL
ETKPVNEGKFGLELLAKLANIKKSKNQKEIDHRKEIGLPVPY
>Mature_282_residues
MDAFGNYKSVISARIADKAVLKKVQDGGIVTAAFVYGLENNLLDGAVVASVGEEFRAVPKVATTVEEILEAAGTKYTTCP
NLSVLKQAIREYGCEKVGVVGTPCQIIATRKSLKYPVGFRHMNDKIALTVGIFCMENFPYNGMKTIVEEHCGVKMDDVAK
LDIGKGKFWTYTKWGETKSIKLADTHPYEQIACHVCTDYTAELADISTGSVGSPDGWSTVFARTEKGEEFLNGMVESGYL
ETKPVNEGKFGLELLAKLANIKKSKNQKEIDHRKEIGLPVPY

Specific function: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen [H]

COG id: COG1035

COG function: function code C; Coenzyme F420-reducing hydrogenase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the frhB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007516
- InterPro:   IPR017679
- InterPro:   IPR007525 [H]

Pfam domain/function: PF04432 FrhB_FdhB_C; PF04422 FrhB_FdhB_N [H]

EC number: =1.12.98.1 [H]

Molecular weight: Translated: 30890; Mature: 30890

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAFGNYKSVISARIADKAVLKKVQDGGIVTAAFVYGLENNLLDGAVVASVGEEFRAVPK
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
VATTVEEILEAAGTKYTTCPNLSVLKQAIREYGCEKVGVVGTPCQIIATRKSLKYPVGFR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHEEEEHHHCCCCCCEE
HMNDKIALTVGIFCMENFPYNGMKTIVEEHCGVKMDDVAKLDIGKGKFWTYTKWGETKSI
ECCCEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCHHEEECCCCCEEEEEECCCCCEE
KLADTHPYEQIACHVCTDYTAELADISTGSVGSPDGWSTVFARTEKGEEFLNGMVESGYL
EEECCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCHHHHEECCCHHHHHHHHHHCCCE
ETKPVNEGKFGLELLAKLANIKKSKNQKEIDHRKEIGLPVPY
ECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDAFGNYKSVISARIADKAVLKKVQDGGIVTAAFVYGLENNLLDGAVVASVGEEFRAVPK
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
VATTVEEILEAAGTKYTTCPNLSVLKQAIREYGCEKVGVVGTPCQIIATRKSLKYPVGFR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHEEEEHHHCCCCCCEE
HMNDKIALTVGIFCMENFPYNGMKTIVEEHCGVKMDDVAKLDIGKGKFWTYTKWGETKSI
ECCCEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCHHEEECCCCCEEEEEECCCCCEE
KLADTHPYEQIACHVCTDYTAELADISTGSVGSPDGWSTVFARTEKGEEFLNGMVESGYL
EEECCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCHHHHEECCCHHHHHHHHHHCCCE
ETKPVNEGKFGLELLAKLANIKKSKNQKEIDHRKEIGLPVPY
ECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]