Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 150401431

GI number: 150401431

Start: 1033698

End: 1035002

Strand: Direct

Name: gatA [H]

Synonym: Maeo_1007

Alternate gene names: 150401431

Gene position: 1033698-1035002 (Clockwise)

Preceding gene: 150401430

Following gene: 150401432

Centisome position: 65.86

GC content: 33.1

Gene sequence:

>1305_bases
ATGACAATTATAGATAAAGCTCTGAAATACATCGAAAAAATTGAAAAATCAGACATAAATGCAATTATTCAACTAGATAA
AGAAAAAGTATTAAATGAAGCCAAAGAGTTGGAAAACAACGAAAAATTGAAAAATAAGCCACTATATGGTAAAATAATTG
CCATCAAATCCAATATAAATGTAAAAGGATATAATATATCTTGTGCTTCAAAAACCCTCGAAAACTATATAAGTGCATAT
GATGCCACAGTGGTCAAAAAAATAAAATCCCAAGGAGGACTAATAATAGGAATGACCAATATGGATGAATTTGCAAGCGG
AAGTAGTGGTGAAACCTCATATTATGGAGCTACAAAAAACCCAAATGCAGAAGGCAAAATTCCAGGGGGTAGTAGTTCAG
GAAGTGCCTCTGCTGTTGCAGGGGATTTGTGTGATATGGCATTGGGAAGCGATACAGGAGGAAGTATTAGAAATCCAGCT
TCCCACTGTGGAGTAGTTGGTTTTAAGCCATCTTATGGTGTAGTGAGCCGACAAGGATTATGTGATTTAGCAATGAGTTT
TGACCAGATTGGACCACTGACAAAAAGTGCCGAAGATGCTTTATTATTAACAAACATAATAAAAGGAAAAGACTTATCCG
ACAGCACCACAGTAGAAACCCCCAAATTTGAAAAAAATGAAAAACAGGTTAAAAAATACAAAGTGGGAATTGTAAAGGAA
TTTATGGAAGTTAGCGATGAAAAAATTAGAAATAAAATAGAAGAAGGGATTGAAGTATTTAAAGACATGGGCTGCAAAAT
TGTAGAATTAAATTATAAATATACCGATTTAGCACTTCCAACATATTATTTAATTAATTATGTGGAATTCTTTTCAGCTA
CAAGAAAATATGATGGTAGAAGATATGGATATCCAATAGAAGAAGTATGCGGTGAAGAAGTTCTTAGGAGAATTCTAATA
GGAAAACATATCAGCGAACAGGAGTTTAGTGGAAAATACTATAAGAAGGCACTATATACAAGGAAATTAATGAAAAACGA
AATGTTAAAACTATTTAACGATGTAGATATAATTGTCAGCCCAACTGTTCCAAAATTGCCACATAATATTGGGAAAGAAT
TGACACCAATGGAAATGTATTCCTACGATGTTTTAACTGTGCCCACAAATATTTGCGGAATCTGTGCAGGAGTAGTTAAA
TGCGGAAACATAGCAGGTAATCCAGTAGGACTACAAATACAAGGAAAACCATTTGATGATGAAAAAGTGCTTAATGCTAT
GATAGAATTTGAAAAACAATATTAA

Upstream 100 bases:

>100_bases
GTATGTTCAAAAAGTATGTTTTTTAAATGTTCAAAAATATACTATATAGTCCCTGCATATATTGTTATATTGTTAAGCGG
TTATAAATGGTGATTAATTA

Downstream 100 bases:

>100_bases
TTACATATTATGGAATTATTATTTTATTATTTATTTTATTTTAATATTGGGAAAATATGGACGAAAGAGCGGTTTTAGAA
GAGTTAAAGAAATATAGAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTIIDKALKYIEKIEKSDINAIIQLDKEKVLNEAKELENNEKLKNKPLYGKIIAIKSNINVKGYNISCASKTLENYISAY
DATVVKKIKSQGGLIIGMTNMDEFASGSSGETSYYGATKNPNAEGKIPGGSSSGSASAVAGDLCDMALGSDTGGSIRNPA
SHCGVVGFKPSYGVVSRQGLCDLAMSFDQIGPLTKSAEDALLLTNIIKGKDLSDSTTVETPKFEKNEKQVKKYKVGIVKE
FMEVSDEKIRNKIEEGIEVFKDMGCKIVELNYKYTDLALPTYYLINYVEFFSATRKYDGRRYGYPIEEVCGEEVLRRILI
GKHISEQEFSGKYYKKALYTRKLMKNEMLKLFNDVDIIVSPTVPKLPHNIGKELTPMEMYSYDVLTVPTNICGICAGVVK
CGNIAGNPVGLQIQGKPFDDEKVLNAMIEFEKQY

Sequences:

>Translated_434_residues
MTIIDKALKYIEKIEKSDINAIIQLDKEKVLNEAKELENNEKLKNKPLYGKIIAIKSNINVKGYNISCASKTLENYISAY
DATVVKKIKSQGGLIIGMTNMDEFASGSSGETSYYGATKNPNAEGKIPGGSSSGSASAVAGDLCDMALGSDTGGSIRNPA
SHCGVVGFKPSYGVVSRQGLCDLAMSFDQIGPLTKSAEDALLLTNIIKGKDLSDSTTVETPKFEKNEKQVKKYKVGIVKE
FMEVSDEKIRNKIEEGIEVFKDMGCKIVELNYKYTDLALPTYYLINYVEFFSATRKYDGRRYGYPIEEVCGEEVLRRILI
GKHISEQEFSGKYYKKALYTRKLMKNEMLKLFNDVDIIVSPTVPKLPHNIGKELTPMEMYSYDVLTVPTNICGICAGVVK
CGNIAGNPVGLQIQGKPFDDEKVLNAMIEFEKQY
>Mature_433_residues
TIIDKALKYIEKIEKSDINAIIQLDKEKVLNEAKELENNEKLKNKPLYGKIIAIKSNINVKGYNISCASKTLENYISAYD
ATVVKKIKSQGGLIIGMTNMDEFASGSSGETSYYGATKNPNAEGKIPGGSSSGSASAVAGDLCDMALGSDTGGSIRNPAS
HCGVVGFKPSYGVVSRQGLCDLAMSFDQIGPLTKSAEDALLLTNIIKGKDLSDSTTVETPKFEKNEKQVKKYKVGIVKEF
MEVSDEKIRNKIEEGIEVFKDMGCKIVELNYKYTDLALPTYYLINYVEFFSATRKYDGRRYGYPIEEVCGEEVLRRILIG
KHISEQEFSGKYYKKALYTRKLMKNEMLKLFNDVDIIVSPTVPKLPHNIGKELTPMEMYSYDVLTVPTNICGICAGVVKC
GNIAGNPVGLQIQGKPFDDEKVLNAMIEFEKQY

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=482, Percent_Identity=34.0248962655602, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI195972892, Length=474, Percent_Identity=24.8945147679325, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17543272, Length=400, Percent_Identity=33.75, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17537465, Length=463, Percent_Identity=23.7580993520518, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=262, Percent_Identity=27.4809160305344, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=278, Percent_Identity=24.8201438848921, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=265, Percent_Identity=26.0377358490566, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=277, Percent_Identity=25.2707581227437, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=174, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538254, Length=496, Percent_Identity=20.7661290322581, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=429, Percent_Identity=34.2657342657343, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6319685, Length=421, Percent_Identity=28.5035629453682, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648113, Length=456, Percent_Identity=33.1140350877193, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24648435, Length=470, Percent_Identity=27.4468085106383, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648437, Length=470, Percent_Identity=27.4468085106383, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI45550774, Length=470, Percent_Identity=27.4468085106383, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21356731, Length=213, Percent_Identity=29.5774647887324, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648441, Length=426, Percent_Identity=26.5258215962441, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648439, Length=426, Percent_Identity=26.5258215962441, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI161078093, Length=477, Percent_Identity=25.9958071278826, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652985, Length=465, Percent_Identity=23.2258064516129, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922090, Length=465, Percent_Identity=23.2258064516129, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652981, Length=465, Percent_Identity=23.2258064516129, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652983, Length=465, Percent_Identity=23.2258064516129, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644968, Length=468, Percent_Identity=24.1452991452991, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 48010; Mature: 47879

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIDKALKYIEKIEKSDINAIIQLDKEKVLNEAKELENNEKLKNKPLYGKIIAIKSNIN
CCHHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHCCCHHHCCCCCEEEEEEEECCCE
VKGYNISCASKTLENYISAYDATVVKKIKSQGGLIIGMTNMDEFASGSSGETSYYGATKN
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCCCCCCCCCCC
PNAEGKIPGGSSSGSASAVAGDLCDMALGSDTGGSIRNPASHCGVVGFKPSYGVVSRQGL
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCCEECCCCCCCCCCCCH
CDLAMSFDQIGPLTKSAEDALLLTNIIKGKDLSDSTTVETPKFEKNEKQVKKYKVGIVKE
HHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
FMEVSDEKIRNKIEEGIEVFKDMGCKIVELNYKYTDLALPTYYLINYVEFFSATRKYDGR
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEHHCHHHHHHHHHHHHHHHHHHCCCC
RYGYPIEEVCGEEVLRRILIGKHISEQEFSGKYYKKALYTRKLMKNEMLKLFNDVDIIVS
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
PTVPKLPHNIGKELTPMEMYSYDVLTVPTNICGICAGVVKCGNIAGNPVGLQIQGKPFDD
CCCCCCCHHHCCCCCCHHHHCCEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCH
EKVLNAMIEFEKQY
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TIIDKALKYIEKIEKSDINAIIQLDKEKVLNEAKELENNEKLKNKPLYGKIIAIKSNIN
CHHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHCCCHHHCCCCCEEEEEEEECCCE
VKGYNISCASKTLENYISAYDATVVKKIKSQGGLIIGMTNMDEFASGSSGETSYYGATKN
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCCCCCCCCCCC
PNAEGKIPGGSSSGSASAVAGDLCDMALGSDTGGSIRNPASHCGVVGFKPSYGVVSRQGL
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCCEECCCCCCCCCCCCH
CDLAMSFDQIGPLTKSAEDALLLTNIIKGKDLSDSTTVETPKFEKNEKQVKKYKVGIVKE
HHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
FMEVSDEKIRNKIEEGIEVFKDMGCKIVELNYKYTDLALPTYYLINYVEFFSATRKYDGR
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEHHCHHHHHHHHHHHHHHHHHHCCCC
RYGYPIEEVCGEEVLRRILIGKHISEQEFSGKYYKKALYTRKLMKNEMLKLFNDVDIIVS
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
PTVPKLPHNIGKELTPMEMYSYDVLTVPTNICGICAGVVKCGNIAGNPVGLQIQGKPFDD
CCCCCCCHHHCCCCCCHHHHCCEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCH
EKVLNAMIEFEKQY
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA