Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 150401410

GI number: 150401410

Start: 1011862

End: 1013208

Strand: Direct

Name: purB [H]

Synonym: Maeo_0986

Alternate gene names: 150401410

Gene position: 1011862-1013208 (Clockwise)

Preceding gene: 150401409

Following gene: 150401411

Centisome position: 64.47

GC content: 35.71

Gene sequence:

>1347_bases
ATGGCAGTGCATCCTATTGATTTTAGATATGGTTCAAAAGAAATGAAAGAAGTTTGGGAAGAAGAAACAAAACTTCAAAA
AATGCTTGAAGTTGAGGCAGCTATTGCAATGGCACAGGGAGAATTAGGTATAATTCCTATGGAAGCTGCTGAGGAAATAA
ATAGAAAGGCGTCAACAAAATTTGTAAGCCTTGAAAGAGCAAAAGAGATTGAAATGGAAACCAGACACGATATAGTATCA
ATGGTTAAGGCTTTTGCGGAAGTTTGTGATAATGGTGCAGGAGAATATATCCATTTTGGAGCTACTTCAAATGATATAGT
GGATACATCCCAATCACTTCAATTTAAAGATGCTGTAAAAATATTGTTAGAAAAATTAGAGACATTAAAAGGGGAGCTCC
TTGCAAAAGCAGAGGAACACAAAACAACAGTATGTATAGGTAGAACACATGGACAGCATGCCATACCTACAACATACGGA
ATGAAATTCGCATTATGGGCTTCGGAGCTCCAAAGACATATTGACAGATTAAACAGCTGTAAAGATAGATTATGTGTTTC
CATGATTACGGGAGCCGTTGGAACTATGGCGGCTCTCGGAGAAAATGGAATGACAATTCATAAAAGAGTAGGGGAGCTCC
TTGGTTTAACTCCTGTTTTAATCTCCAATCAAGTAATCCAAAGAGATAGACACGGGGAATTTATGGCAATTCTCGCCTTA
ATTGCTCAAACACTCAATAAAATAGGTATAACTGTAAGAAGCATGCAAAGAACAGAAATTAAAGAATTGGAAGAAGAATT
CGATGCTTCAAAACAAACTGGGAGCTCCACGATGCCACACAAAAGAAATCCTATTACATTTGAGCAAATTTGCGGTTTAT
CCAGAGTTGTAAAGGCTAATGCACTTGGAGAGTTTGACAACATTCCATTGTGGGAAGAAAGAGATTTAACAAATTCCTCA
GCTGAAAGATGTTTATTCCCTGAATCCTGTGTATTGTTAGACCATATATTAAATTTATCAATAAAAGGAATGAGAAAATT
AACAGTGAATCTCCAAAATGTTCAAAGAAACCTTGAAGCTACAAACGGGCTTATTATGGCAGAAAGAGCTATGATTACTT
TGGCAAATAAAGGAATGGGAAGACAGACCGCCTATGGTGTTGTTAGAGTTTGTGCTATGAAGTCATTTGAGGAAAACAGA
CATTTAAAAGAAGTGTTAATGGAAGATGCCGAAGTAATGAAGTTTATGAATAAAGAAGAACTTGACAGCTTATTTGATTA
TTCAACATACATAGGATTAGCCCCTGAAATTGTTGAAAATGTGATTAATGAATTAAAAAATAAATAA

Upstream 100 bases:

>100_bases
CAAAAACAGAAGATATAAAAATTAAAAATTCACGGTGAAAACATATATAAAATCAAAGATTTTATATTTTTTCACTTAAA
AAGATAAAGAGCGTGAAAAA

Downstream 100 bases:

>100_bases
AAATAATAAAAAATGATATTATGGACTGGATAAAAGAATATTTACATATTCTCCAATGTCCTTTTTGTGGGGGAGATTTG
GGGCTTCAAAATAATAAATT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MAVHPIDFRYGSKEMKEVWEEETKLQKMLEVEAAIAMAQGELGIIPMEAAEEINRKASTKFVSLERAKEIEMETRHDIVS
MVKAFAEVCDNGAGEYIHFGATSNDIVDTSQSLQFKDAVKILLEKLETLKGELLAKAEEHKTTVCIGRTHGQHAIPTTYG
MKFALWASELQRHIDRLNSCKDRLCVSMITGAVGTMAALGENGMTIHKRVGELLGLTPVLISNQVIQRDRHGEFMAILAL
IAQTLNKIGITVRSMQRTEIKELEEEFDASKQTGSSTMPHKRNPITFEQICGLSRVVKANALGEFDNIPLWEERDLTNSS
AERCLFPESCVLLDHILNLSIKGMRKLTVNLQNVQRNLEATNGLIMAERAMITLANKGMGRQTAYGVVRVCAMKSFEENR
HLKEVLMEDAEVMKFMNKEELDSLFDYSTYIGLAPEIVENVINELKNK

Sequences:

>Translated_448_residues
MAVHPIDFRYGSKEMKEVWEEETKLQKMLEVEAAIAMAQGELGIIPMEAAEEINRKASTKFVSLERAKEIEMETRHDIVS
MVKAFAEVCDNGAGEYIHFGATSNDIVDTSQSLQFKDAVKILLEKLETLKGELLAKAEEHKTTVCIGRTHGQHAIPTTYG
MKFALWASELQRHIDRLNSCKDRLCVSMITGAVGTMAALGENGMTIHKRVGELLGLTPVLISNQVIQRDRHGEFMAILAL
IAQTLNKIGITVRSMQRTEIKELEEEFDASKQTGSSTMPHKRNPITFEQICGLSRVVKANALGEFDNIPLWEERDLTNSS
AERCLFPESCVLLDHILNLSIKGMRKLTVNLQNVQRNLEATNGLIMAERAMITLANKGMGRQTAYGVVRVCAMKSFEENR
HLKEVLMEDAEVMKFMNKEELDSLFDYSTYIGLAPEIVENVINELKNK
>Mature_447_residues
AVHPIDFRYGSKEMKEVWEEETKLQKMLEVEAAIAMAQGELGIIPMEAAEEINRKASTKFVSLERAKEIEMETRHDIVSM
VKAFAEVCDNGAGEYIHFGATSNDIVDTSQSLQFKDAVKILLEKLETLKGELLAKAEEHKTTVCIGRTHGQHAIPTTYGM
KFALWASELQRHIDRLNSCKDRLCVSMITGAVGTMAALGENGMTIHKRVGELLGLTPVLISNQVIQRDRHGEFMAILALI
AQTLNKIGITVRSMQRTEIKELEEEFDASKQTGSSTMPHKRNPITFEQICGLSRVVKANALGEFDNIPLWEERDLTNSSA
ERCLFPESCVLLDHILNLSIKGMRKLTVNLQNVQRNLEATNGLIMAERAMITLANKGMGRQTAYGVVRVCAMKSFEENRH
LKEVLMEDAEVMKFMNKEELDSLFDYSTYIGLAPEIVENVINELKNK

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=453, Percent_Identity=27.3730684326711, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI183227688, Length=387, Percent_Identity=28.1653746770026, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1787376, Length=407, Percent_Identity=28.7469287469287, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=373, Percent_Identity=28.9544235924933, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564234, Length=306, Percent_Identity=29.7385620915033, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17553882, Length=215, Percent_Identity=30.6976744186047, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI32565146, Length=208, Percent_Identity=32.2115384615385, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323391, Length=455, Percent_Identity=27.6923076923077, Blast_Score=162, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24647570, Length=455, Percent_Identity=27.2527472527473, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24640179, Length=220, Percent_Identity=31.8181818181818, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24640177, Length=220, Percent_Identity=31.8181818181818, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24662684, Length=219, Percent_Identity=28.310502283105, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI78710009, Length=225, Percent_Identity=29.7777777777778, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50293; Mature: 50162

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVHPIDFRYGSKEMKEVWEEETKLQKMLEVEAAIAMAQGELGIIPMEAAEEINRKASTK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHH
FVSLERAKEIEMETRHDIVSMVKAFAEVCDNGAGEYIHFGATSNDIVDTSQSLQFKDAVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHCCHHHHHHHHHHH
ILLEKLETLKGELLAKAEEHKTTVCIGRTHGQHAIPTTYGMKFALWASELQRHIDRLNSC
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHH
KDRLCVSMITGAVGTMAALGENGMTIHKRVGELLGLTPVLISNQVIQRDRHGEFMAILAL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHH
IAQTLNKIGITVRSMQRTEIKELEEEFDASKQTGSSTMPHKRNPITFEQICGLSRVVKAN
HHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCHHHHHCHHHHHHHH
ALGEFDNIPLWEERDLTNSSAERCLFPESCVLLDHILNLSIKGMRKLTVNLQNVQRNLEA
CCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHH
TNGLIMAERAMITLANKGMGRQTAYGVVRVCAMKSFEENRHLKEVLMEDAEVMKFMNKEE
HCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
LDSLFDYSTYIGLAPEIVENVINELKNK
HHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVHPIDFRYGSKEMKEVWEEETKLQKMLEVEAAIAMAQGELGIIPMEAAEEINRKASTK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHH
FVSLERAKEIEMETRHDIVSMVKAFAEVCDNGAGEYIHFGATSNDIVDTSQSLQFKDAVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHCCHHHHHHHHHHH
ILLEKLETLKGELLAKAEEHKTTVCIGRTHGQHAIPTTYGMKFALWASELQRHIDRLNSC
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHH
KDRLCVSMITGAVGTMAALGENGMTIHKRVGELLGLTPVLISNQVIQRDRHGEFMAILAL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHH
IAQTLNKIGITVRSMQRTEIKELEEEFDASKQTGSSTMPHKRNPITFEQICGLSRVVKAN
HHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCHHHHHCHHHHHHHH
ALGEFDNIPLWEERDLTNSSAERCLFPESCVLLDHILNLSIKGMRKLTVNLQNVQRNLEA
CCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHH
TNGLIMAERAMITLANKGMGRQTAYGVVRVCAMKSFEENRHLKEVLMEDAEVMKFMNKEE
HCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
LDSLFDYSTYIGLAPEIVENVINELKNK
HHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]