Click here to switch to the map view.

The map label for this gene is thiI [H]

Identifier: 150401225

GI number: 150401225

Start: 823594

End: 824814

Strand: Reverse

Name: thiI [H]

Synonym: Maeo_0796

Alternate gene names: 150401225

Gene position: 824814-823594 (Counterclockwise)

Preceding gene: 150401231

Following gene: 150401221

Centisome position: 52.55

GC content: 27.52

Gene sequence:

>1221_bases
TTGAATAATAATAATGAAGATATTGATAATAATAATATTATTAAAGTAATAGTAAGATATGGCGAGATTGGAACAAAGGC
AAGAGGGACAAGAAAAAGATTTGAACAAACCCTTAAAGAAAATATTAAAAATACACTTAAAAAATATAATATTTATTCGG
AGGTCGATATATTACATACAAGATTATTATTGACCATAAATAAGGATAATTTAGATAAATCTCTAGAATTATTGGGCAAA
ATATCTGGTATTGTATCATACAGTCCAATTTTGGAATGTAATTTAGATATAAATGAAATTGAAAAATTGGCAATAGAAGT
ATTTGATAAAGAAATTAATACTGAAACAAACAAAACAAAAGATATTATTACATTTGCCGTAAAAACCCAGAGAGTTAAGA
AAAAATTTAAAATGAATTCAATGGAATTAAGTGCCCATATTGGGAGCTCCATAGCATTACGATATGAAGACAGCCCTATA
AATTTAAAAGTAGATTTAAAAAATCCAGATATATTGTTAAATATTGAGTTAATGGATAATGCAATGATATTTACAAAAAA
AATTAGGGGAATTGGTGGAATACCTGTTGGAACACAGGGAAAAGTGTTGGTTTTATTGTCTGATGGGATAGATAGTTCCG
TAGCATCCTATTATATTATTAAAAGGGGCTGCAATGCTGTATTTTTACATATGAAATTATCCGAAGAAGGACTCGAAAAA
ACTAAAAAATTATGTGGCGTATTAAATGATTATGATTGTAATTCTGAATTGATTGTTGTAGATTATAAAGAGGAATTAAA
AAATATAGTTAGCAATTTAAAGAAAATAAATCGAGAAAAATATGCCTGTGTGTTCTGTAAAAAAACAATGTTAAAAATCG
CAGAAAAATATGCAAAAGAAAATAATTGTCATGCCATAGTGAATGGAGATAATTTGGGACAGGTTGCATCTCAAACTTTA
AAAAATCTTGCTGTGATTAGTGAAGGTGTGGAGCTCCCAATACTTAGGCCACTTATAGGATTTGATAAACAGGAAATAAT
AAATAAGGCAAAAGAAATCGGCACATATGAAATATCGATAAGTAAAGAAATAAAATGTTTTGCAGTTCCTATTCATCCAA
TAACCAATGCAGATATTCGAGAAATTAGATATATTGAGGAAAGATTGGAAAGAAATGCAAAAAAAGAAGAGATAAAAGAA
GAAGTAGAAAATAATGAATAA

Upstream 100 bases:

>100_bases
CGTTTATATTTTCACAGGCTATATGGATAATATATTATATTTATATATATTATATATAACTATACTCCTATATAATATAT
AAAATATATCAGGTGAAAAA

Downstream 100 bases:

>100_bases
TATAAAAATACAATAAAATAAGAATTAAATTAAACTCTTATTCCTAAATTTTCCAAAAGGGCACATGATTTACATATTTC
GGAGCTCCCAGCTTCACCAC

Product: thiamine biosynthesis/tRNA modification protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MNNNNEDIDNNNIIKVIVRYGEIGTKARGTRKRFEQTLKENIKNTLKKYNIYSEVDILHTRLLLTINKDNLDKSLELLGK
ISGIVSYSPILECNLDINEIEKLAIEVFDKEINTETNKTKDIITFAVKTQRVKKKFKMNSMELSAHIGSSIALRYEDSPI
NLKVDLKNPDILLNIELMDNAMIFTKKIRGIGGIPVGTQGKVLVLLSDGIDSSVASYYIIKRGCNAVFLHMKLSEEGLEK
TKKLCGVLNDYDCNSELIVVDYKEELKNIVSNLKKINREKYACVFCKKTMLKIAEKYAKENNCHAIVNGDNLGQVASQTL
KNLAVISEGVELPILRPLIGFDKQEIINKAKEIGTYEISISKEIKCFAVPIHPITNADIREIRYIEERLERNAKKEEIKE
EVENNE

Sequences:

>Translated_406_residues
MNNNNEDIDNNNIIKVIVRYGEIGTKARGTRKRFEQTLKENIKNTLKKYNIYSEVDILHTRLLLTINKDNLDKSLELLGK
ISGIVSYSPILECNLDINEIEKLAIEVFDKEINTETNKTKDIITFAVKTQRVKKKFKMNSMELSAHIGSSIALRYEDSPI
NLKVDLKNPDILLNIELMDNAMIFTKKIRGIGGIPVGTQGKVLVLLSDGIDSSVASYYIIKRGCNAVFLHMKLSEEGLEK
TKKLCGVLNDYDCNSELIVVDYKEELKNIVSNLKKINREKYACVFCKKTMLKIAEKYAKENNCHAIVNGDNLGQVASQTL
KNLAVISEGVELPILRPLIGFDKQEIINKAKEIGTYEISISKEIKCFAVPIHPITNADIREIRYIEERLERNAKKEEIKE
EVENNE
>Mature_406_residues
MNNNNEDIDNNNIIKVIVRYGEIGTKARGTRKRFEQTLKENIKNTLKKYNIYSEVDILHTRLLLTINKDNLDKSLELLGK
ISGIVSYSPILECNLDINEIEKLAIEVFDKEINTETNKTKDIITFAVKTQRVKKKFKMNSMELSAHIGSSIALRYEDSPI
NLKVDLKNPDILLNIELMDNAMIFTKKIRGIGGIPVGTQGKVLVLLSDGIDSSVASYYIIKRGCNAVFLHMKLSEEGLEK
TKKLCGVLNDYDCNSELIVVDYKEELKNIVSNLKKINREKYACVFCKKTMLKIAEKYAKENNCHAIVNGDNLGQVASQTL
KNLAVISEGVELPILRPLIGFDKQEIINKAKEIGTYEISISKEIKCFAVPIHPITNADIREIRYIEERLERNAKKEEIKE
EVENNE

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=387, Percent_Identity=29.1989664082687, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 46142; Mature: 46142

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNNEDIDNNNIIKVIVRYGEIGTKARGTRKRFEQTLKENIKNTLKKYNIYSEVDILHT
CCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEE
RLLLTINKDNLDKSLELLGKISGIVSYSPILECNLDINEIEKLAIEVFDKEINTETNKTK
EEEEEECCCCHHHHHHHHHHHHHHHEECCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCH
DIITFAVKTQRVKKKFKMNSMELSAHIGSSIALRYEDSPINLKVDLKNPDILLNIELMDN
HHEEEHHHHHHHHHHHCCCCEEEHEECCCEEEEEEECCCEEEEEEECCCCEEEEEEEECC
AMIFTKKIRGIGGIPVGTQGKVLVLLSDGIDSSVASYYIIKRGCNAVFLHMKLSEEGLEK
CCEEHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEEEECHHHHHH
TKKLCGVLNDYDCNSELIVVDYKEELKNIVSNLKKINREKYACVFCKKTMLKIAEKYAKE
HHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
NNCHAIVNGDNLGQVASQTLKNLAVISEGVELPILRPLIGFDKQEIINKAKEIGTYEISI
CCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHCCEEEEEE
SKEIKCFAVPIHPITNADIREIRYIEERLERNAKKEEIKEEVENNE
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNNNNEDIDNNNIIKVIVRYGEIGTKARGTRKRFEQTLKENIKNTLKKYNIYSEVDILHT
CCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEE
RLLLTINKDNLDKSLELLGKISGIVSYSPILECNLDINEIEKLAIEVFDKEINTETNKTK
EEEEEECCCCHHHHHHHHHHHHHHHEECCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCH
DIITFAVKTQRVKKKFKMNSMELSAHIGSSIALRYEDSPINLKVDLKNPDILLNIELMDN
HHEEEHHHHHHHHHHHCCCCEEEHEECCCEEEEEEECCCEEEEEEECCCCEEEEEEEECC
AMIFTKKIRGIGGIPVGTQGKVLVLLSDGIDSSVASYYIIKRGCNAVFLHMKLSEEGLEK
CCEEHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEEEECHHHHHH
TKKLCGVLNDYDCNSELIVVDYKEELKNIVSNLKKINREKYACVFCKKTMLKIAEKYAKE
HHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
NNCHAIVNGDNLGQVASQTLKNLAVISEGVELPILRPLIGFDKQEIINKAKEIGTYEISI
CCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHCCEEEEEE
SKEIKCFAVPIHPITNADIREIRYIEERLERNAKKEEIKEEVENNE
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]