Click here to switch to the map view.

The map label for this gene is selB [H]

Identifier: 150401205

GI number: 150401205

Start: 798388

End: 799794

Strand: Reverse

Name: selB [H]

Synonym: Maeo_0776

Alternate gene names: 150401205

Gene position: 799794-798388 (Counterclockwise)

Preceding gene: 150401210

Following gene: 150401204

Centisome position: 50.96

GC content: 31.27

Gene sequence:

>1407_bases
ATGAAAAATATAAATTTAGGAATATTTGGACACATTGACCACGGAAAAACGACATTAGCAAGAGTTTTAACAGAAATAGC
ATCAACTTCATCATTGGATAAATTACCAGAATCAAAAAAACGAGGAATTACAATTGATATAGGTTTTTCTTCATTTAATA
TGCCCGATTATATTATCACATTGGTGGATGCACCAGGACATGCCGACTTAATAAAGGCAGTAGTTAGTGCAGCAGATATA
ATAGATCTGGCAATATTGGTTGTAGATGCAAAAGAAGGACCAAAAACACAAACAGGCGAGCATTTACTAATATTAGATTA
TTTTAACATTCCTACCATTGCTGTAATTACAAAAATAGATCTGGCAACAGAAGAAGAAATAAAAAGAACAAAATCAATAG
TAAGTGCAGTATTAAATTCTACGGAAAATTTAAAAGATAGTCAAATAATAGAAATATCTGCAAAAGAAAATAAAAATATC
GATAATCTAAAAAATACTATTCATAAAACATTGAATAGTTTGAATATAACAAGGAGCTCCGACGAATATTTTAAGATGCC
AATAGACCACGCCTTCCCAATAAAGGGAATTGGGACAGTAATAACTGGAACAATATTAAAAGGAAAAGTATCCGTAGGGC
AGGATGACTTAAAAATTATGCCAATAAATATGAATAATATAAAGGTAAAAAGCATACAGCGATTTAAAAAGGAAGAAAAG
GAGGCAATGATGGGGGACAGGGTTGGAATGGCACTTCATGGAGTAGATGCAAAGCAAATTTATAGGGGCTGTATTCTCAC
ATCCTCAAATTCTAAATTGGAAATAGTGGATAAAATTGTGGCTAAAATAAAAATGTCCGACATATTTAGATACAACATAA
AACCAAAAATGAAGGTTCATTTAAATGTAGGAATGTTAATTGTTCCAGCAATTGTGGTTCCATTTAAAAAAATCAAATTT
AAAGGAAAGGAGGAAAATATAATATTAAAAGAAATACAAAAAGGGGATGAATGCTACTGTTCATTTGAGCTTGACGAGAA
AGTAATGGCAGAAATAGGGGACAATATACTTATTACAAGGTTGGATTTACCACCTACGACACTAAGAATATGCGGTAGGG
GGGAAATAGTAGAATTTAAATCAATGAAAGACTTGAATATTAAAAAAGAAGTAATTAGAACAGGAAATATAAAAATAGAT
AAAAATAGAGTATTTATAGAGGGGTTGGCAAATTCTAAATCTTCGGCTGAAAAATTAATTGGTGAGGTGGTGGAGCTCCC
AGATAAAAATATAACTGGAAAATTAAAAAACACATTTGGGACAAAAGGATATTTAACGGCTGATTTTGATGGTGAAGTTA
GCAATAAAGATAAAGTAATTTTAAATAGGCTTAGACGGTGGGGATAA

Upstream 100 bases:

>100_bases
TATTTATTGAATATATAATTGTCGATTCACTATATATGTTATATAGATTATACATAATATATATGTTATATATTGGATAT
ATTAGATAAAGGGATATAAT

Downstream 100 bases:

>100_bases
TGAATAATATTGGTAATATTAATAATATTAAATATGACGGCAACAACAATGATAGCAAAATAACCACAATAGAAACGCCT
TCAAAAGTCATATTATTTGG

Product: selenocysteine-specific translation elongation factor

Products: GDP; phosphate

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MKNINLGIFGHIDHGKTTLARVLTEIASTSSLDKLPESKKRGITIDIGFSSFNMPDYIITLVDAPGHADLIKAVVSAADI
IDLAILVVDAKEGPKTQTGEHLLILDYFNIPTIAVITKIDLATEEEIKRTKSIVSAVLNSTENLKDSQIIEISAKENKNI
DNLKNTIHKTLNSLNITRSSDEYFKMPIDHAFPIKGIGTVITGTILKGKVSVGQDDLKIMPINMNNIKVKSIQRFKKEEK
EAMMGDRVGMALHGVDAKQIYRGCILTSSNSKLEIVDKIVAKIKMSDIFRYNIKPKMKVHLNVGMLIVPAIVVPFKKIKF
KGKEENIILKEIQKGDECYCSFELDEKVMAEIGDNILITRLDLPPTTLRICGRGEIVEFKSMKDLNIKKEVIRTGNIKID
KNRVFIEGLANSKSSAEKLIGEVVELPDKNITGKLKNTFGTKGYLTADFDGEVSNKDKVILNRLRRWG

Sequences:

>Translated_468_residues
MKNINLGIFGHIDHGKTTLARVLTEIASTSSLDKLPESKKRGITIDIGFSSFNMPDYIITLVDAPGHADLIKAVVSAADI
IDLAILVVDAKEGPKTQTGEHLLILDYFNIPTIAVITKIDLATEEEIKRTKSIVSAVLNSTENLKDSQIIEISAKENKNI
DNLKNTIHKTLNSLNITRSSDEYFKMPIDHAFPIKGIGTVITGTILKGKVSVGQDDLKIMPINMNNIKVKSIQRFKKEEK
EAMMGDRVGMALHGVDAKQIYRGCILTSSNSKLEIVDKIVAKIKMSDIFRYNIKPKMKVHLNVGMLIVPAIVVPFKKIKF
KGKEENIILKEIQKGDECYCSFELDEKVMAEIGDNILITRLDLPPTTLRICGRGEIVEFKSMKDLNIKKEVIRTGNIKID
KNRVFIEGLANSKSSAEKLIGEVVELPDKNITGKLKNTFGTKGYLTADFDGEVSNKDKVILNRLRRWG
>Mature_468_residues
MKNINLGIFGHIDHGKTTLARVLTEIASTSSLDKLPESKKRGITIDIGFSSFNMPDYIITLVDAPGHADLIKAVVSAADI
IDLAILVVDAKEGPKTQTGEHLLILDYFNIPTIAVITKIDLATEEEIKRTKSIVSAVLNSTENLKDSQIIEISAKENKNI
DNLKNTIHKTLNSLNITRSSDEYFKMPIDHAFPIKGIGTVITGTILKGKVSVGQDDLKIMPINMNNIKVKSIQRFKKEEK
EAMMGDRVGMALHGVDAKQIYRGCILTSSNSKLEIVDKIVAKIKMSDIFRYNIKPKMKVHLNVGMLIVPAIVVPFKKIKF
KGKEENIILKEIQKGDECYCSFELDEKVMAEIGDNILITRLDLPPTTLRICGRGEIVEFKSMKDLNIKKEVIRTGNIKID
KNRVFIEGLANSKSSAEKLIGEVVELPDKNITGKLKNTFGTKGYLTADFDGEVSNKDKVILNRLRRWG

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI54607086, Length=340, Percent_Identity=35.5882352941176, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI34147630, Length=291, Percent_Identity=27.4914089347079, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI223555963, Length=357, Percent_Identity=27.1708683473389, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI5729864, Length=357, Percent_Identity=27.1708683473389, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4503475, Length=331, Percent_Identity=27.4924471299094, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI4503471, Length=326, Percent_Identity=26.9938650306748, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI157426893, Length=175, Percent_Identity=32, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI82546879, Length=324, Percent_Identity=25.3086419753086, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI194018522, Length=317, Percent_Identity=24.2902208201893, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI194018520, Length=317, Percent_Identity=24.2902208201893, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI194097354, Length=317, Percent_Identity=24.2902208201893, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI94966754, Length=174, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI19923640, Length=127, Percent_Identity=34.6456692913386, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI25306283, Length=127, Percent_Identity=34.6456692913386, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI25306287, Length=127, Percent_Identity=34.6456692913386, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI46094014, Length=313, Percent_Identity=24.6006389776358, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI53729339, Length=209, Percent_Identity=27.7511961722488, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI53729337, Length=209, Percent_Identity=27.7511961722488, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI2367247, Length=262, Percent_Identity=33.206106870229, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790412, Length=288, Percent_Identity=31.5972222222222, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1789737, Length=288, Percent_Identity=31.5972222222222, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1789108, Length=261, Percent_Identity=26.0536398467433, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI48994988, Length=125, Percent_Identity=36, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788922, Length=135, Percent_Identity=32.5925925925926, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789559, Length=209, Percent_Identity=31.1004784688995, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17506081, Length=504, Percent_Identity=27.5793650793651, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17556456, Length=307, Percent_Identity=30.6188925081433, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25141371, Length=277, Percent_Identity=32.129963898917, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17506079, Length=502, Percent_Identity=24.3027888446215, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17552884, Length=325, Percent_Identity=26.7692307692308, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17569207, Length=325, Percent_Identity=26.7692307692308, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=169, Percent_Identity=33.1360946745562, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=214, Percent_Identity=27.5700934579439, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115532067, Length=191, Percent_Identity=28.2722513089005, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115532065, Length=191, Percent_Identity=28.2722513089005, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32566301, Length=139, Percent_Identity=30.9352517985612, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=325, Percent_Identity=30.4615384615385, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6325337, Length=318, Percent_Identity=26.4150943396226, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319594, Length=318, Percent_Identity=26.4150943396226, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=173, Percent_Identity=33.5260115606936, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320377, Length=340, Percent_Identity=25.2941176470588, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922690, Length=502, Percent_Identity=31.2749003984064, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI19921738, Length=293, Percent_Identity=33.4470989761092, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281363316, Length=305, Percent_Identity=30.8196721311475, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI17864358, Length=305, Percent_Identity=30.8196721311475, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI45553807, Length=325, Percent_Identity=27.3846153846154, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45553816, Length=325, Percent_Identity=27.3846153846154, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24651721, Length=325, Percent_Identity=27.3846153846154, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17864154, Length=325, Percent_Identity=27.3846153846154, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652838, Length=329, Percent_Identity=27.6595744680851, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137572, Length=329, Percent_Identity=27.6595744680851, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45550900, Length=359, Percent_Identity=24.7910863509749, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI78706572, Length=291, Percent_Identity=23.3676975945017, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 52087; Mature: 52087

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNINLGIFGHIDHGKTTLARVLTEIASTSSLDKLPESKKRGITIDIGFSSFNMPDYIIT
CCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHCCCHHHCCEEEEECCCCCCCCCEEEE
LVDAPGHADLIKAVVSAADIIDLAILVVDAKEGPKTQTGEHLLILDYFNIPTIAVITKID
EEECCCHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEEEEC
LATEEEIKRTKSIVSAVLNSTENLKDSQIIEISAKENKNIDNLKNTIHKTLNSLNITRSS
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCEEECCC
DEYFKMPIDHAFPIKGIGTVITGTILKGKVSVGQDDLKIMPINMNNIKVKSIQRFKKEEK
CCEEECCCCCCCCCCCCCHHHEEEEEECEEECCCCCCEEEEECCCCEEHHHHHHHHHHHH
EAMMGDRVGMALHGVDAKQIYRGCILTSSNSKLEIVDKIVAKIKMSDIFRYNIKPKMKVH
HHHHCCCCCEEEECCCHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCEEEE
LNVGMLIVPAIVVPFKKIKFKGKEENIILKEIQKGDECYCSFELDEKVMAEIGDNILITR
EECCHHHHHHHHHCHHHEEECCCCCCCHHHHHCCCCCEEEEEECCHHHHHHCCCCEEEEE
LDLPPTTLRICGRGEIVEFKSMKDLNIKKEVIRTGNIKIDKNRVFIEGLANSKSSAEKLI
ECCCCCEEEEECCCCEEEECCCCCCCHHHHHHHCCCEEEECCEEEEEECCCCCHHHHHHH
GEVVELPDKNITGKLKNTFGTKGYLTADFDGEVSNKDKVILNRLRRWG
HHHHHCCCCCCCCCHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKNINLGIFGHIDHGKTTLARVLTEIASTSSLDKLPESKKRGITIDIGFSSFNMPDYIIT
CCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHCCCHHHCCEEEEECCCCCCCCCEEEE
LVDAPGHADLIKAVVSAADIIDLAILVVDAKEGPKTQTGEHLLILDYFNIPTIAVITKID
EEECCCHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEEEEC
LATEEEIKRTKSIVSAVLNSTENLKDSQIIEISAKENKNIDNLKNTIHKTLNSLNITRSS
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCEEECCC
DEYFKMPIDHAFPIKGIGTVITGTILKGKVSVGQDDLKIMPINMNNIKVKSIQRFKKEEK
CCEEECCCCCCCCCCCCCHHHEEEEEECEEECCCCCCEEEEECCCCEEHHHHHHHHHHHH
EAMMGDRVGMALHGVDAKQIYRGCILTSSNSKLEIVDKIVAKIKMSDIFRYNIKPKMKVH
HHHHCCCCCEEEECCCHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCEEEE
LNVGMLIVPAIVVPFKKIKFKGKEENIILKEIQKGDECYCSFELDEKVMAEIGDNILITR
EECCHHHHHHHHHCHHHEEECCCCCCCHHHHHCCCCCEEEEEECCHHHHHHCCCCEEEEE
LDLPPTTLRICGRGEIVEFKSMKDLNIKKEVIRTGNIKIDKNRVFIEGLANSKSSAEKLI
ECCCCCEEEEECCCCEEEECCCCCCCHHHHHHHCCCEEEECCEEEEEECCCCCHHHHHHH
GEVVELPDKNITGKLKNTFGTKGYLTADFDGEVSNKDKVILNRLRRWG
HHHHHCCCCCCCCCHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 10860743 [H]