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The map label for this gene is hisF

Identifier: 150401167

GI number: 150401167

Start: 756702

End: 757529

Strand: Reverse

Name: hisF

Synonym: Maeo_0738

Alternate gene names: 150401167

Gene position: 757529-756702 (Counterclockwise)

Preceding gene: 150401168

Following gene: 150401166

Centisome position: 48.27

GC content: 34.9

Gene sequence:

>828_bases
TTGTTAACAAAAAGAATTATACCATGTTTGGACATTAAAAATGGAAGAGTAGTTAAAGGAACAAATTTTGTGGAGCTCCG
TGATGCAGGTGACCCAGTAGAGTTATCAAAAATATACAATGAGCAGGGAGCTGACGAATTAGTATTTTTGGATATTGGGG
CATCTCATGAAAAACGGGATATAATTGAGCATTTAGTTGAAAAAACAGCTGACCAAGTATTTATTCCATTAACCGTTGGA
GGAGGAATAAGAACCACCGACGATTTTAGGAGAATACTTAGGGCAGGAGCTGATAAAATATCAGTTAATACAGCGGCAAT
ACACAATCCAGATTTGATAAAACAATCAAGTGAATTATATGGTGCCCAATGTGTTGTGGTGGCAATTGATTCAAAAAGAC
ATTATGTTAATGAGGAGGATTTAGATTCAAAACAGCTTGAAGATAAAAATGTCGTTAAAGTTGATAAAAATAAATATTGC
TGGTTTGAAGTATTTATTTATGGAGGTAGAAAAGAAACCGGCATTGATGCTATTGAATGGGCTAAAACAGCTGAGGAATT
AGGTGCAGGAGAAATACTTTTAACAAGTATGGACGCAGATGGGACAAAAGACGGATATGACATTGAATTAACTAAATCAA
TTTCTACCCATATAAAATTACCAGTTATAGCAAGTGGTGGTTGCGGAGCTCCCCAGCATATTCATCAGGTATTTGAATAT
GGTAAAGCAGATGCTGCACTTATGGCAAGTATTTTGCATTACAGAGAATACACTGTTGGAGAGTTAAAAAAATATTGTAT
GGATAAAAATATCCCTATGAGATTGTAA

Upstream 100 bases:

>100_bases
TCTATTAATATGCCCTATTCAACTCAATATTTGGGACAATAAAGGGACAGTTTTTGAACTTACTATATAACATATCTATA
AATTATATTTGGTGGATAAA

Downstream 100 bases:

>100_bases
TATATTTTTATTAATTATTTTAATTTTCTTATTTTTGTAGTTTAAGGAAGGGTAGTTTAGGAGCTCCTTGCACTAATTAT
ATTTAATATGCCAATCTTGT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MLTKRIIPCLDIKNGRVVKGTNFVELRDAGDPVELSKIYNEQGADELVFLDIGASHEKRDIIEHLVEKTADQVFIPLTVG
GGIRTTDDFRRILRAGADKISVNTAAIHNPDLIKQSSELYGAQCVVVAIDSKRHYVNEEDLDSKQLEDKNVVKVDKNKYC
WFEVFIYGGRKETGIDAIEWAKTAEELGAGEILLTSMDADGTKDGYDIELTKSISTHIKLPVIASGGCGAPQHIHQVFEY
GKADAALMASILHYREYTVGELKKYCMDKNIPMRL

Sequences:

>Translated_275_residues
MLTKRIIPCLDIKNGRVVKGTNFVELRDAGDPVELSKIYNEQGADELVFLDIGASHEKRDIIEHLVEKTADQVFIPLTVG
GGIRTTDDFRRILRAGADKISVNTAAIHNPDLIKQSSELYGAQCVVVAIDSKRHYVNEEDLDSKQLEDKNVVKVDKNKYC
WFEVFIYGGRKETGIDAIEWAKTAEELGAGEILLTSMDADGTKDGYDIELTKSISTHIKLPVIASGGCGAPQHIHQVFEY
GKADAALMASILHYREYTVGELKKYCMDKNIPMRL
>Mature_275_residues
MLTKRIIPCLDIKNGRVVKGTNFVELRDAGDPVELSKIYNEQGADELVFLDIGASHEKRDIIEHLVEKTADQVFIPLTVG
GGIRTTDDFRRILRAGADKISVNTAAIHNPDLIKQSSELYGAQCVVVAIDSKRHYVNEEDLDSKQLEDKNVVKVDKNKYC
WFEVFIYGGRKETGIDAIEWAKTAEELGAGEILLTSMDADGTKDGYDIELTKSISTHIKLPVIASGGCGAPQHIHQVFEY
GKADAALMASILHYREYTVGELKKYCMDKNIPMRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=275, Percent_Identity=38.5454545454545, Blast_Score=193, Evalue=9e-51,
Organism=Escherichia coli, GI87082028, Length=232, Percent_Identity=27.5862068965517, Blast_Score=77, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=34.7133757961783, Blast_Score=189, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_META3 (A6UUZ9)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324933.1
- ProteinModelPortal:   A6UUZ9
- SMR:   A6UUZ9
- STRING:   A6UUZ9
- GeneID:   5327053
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0738
- eggNOG:   arNOG04606
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   MAEO419665:MAEO_0738-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 30615; Mature: 30615

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDIKNGRVVKGTNFVELRDAGDPVELSKIYNEQGADELVFLDIGASHEKRD
CCCCCCCCEEECCCCEEEECCCEEEECCCCCCCHHHHHHHHCCCCEEEEEEECCCCHHHH
IIEHLVEKTADQVFIPLTVGGGIRTTDDFRRILRAGADKISVNTAAIHNPDLIKQSSELY
HHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHC
GAQCVVVAIDSKRHYVNEEDLDSKQLEDKNVVKVDKNKYCWFEVFIYGGRKETGIDAIEW
CCEEEEEEEECCCCCCCCCCCCHHHCCCCCEEEECCCCEEEEEEEEECCCCCCCCHHHHH
AKTAEELGAGEILLTSMDADGTKDGYDIELTKSISTHIKLPVIASGGCGAPQHIHQVFEY
HHHHHHCCCCEEEEEECCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHC
GKADAALMASILHYREYTVGELKKYCMDKNIPMRL
CCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLTKRIIPCLDIKNGRVVKGTNFVELRDAGDPVELSKIYNEQGADELVFLDIGASHEKRD
CCCCCCCCEEECCCCEEEECCCEEEECCCCCCCHHHHHHHHCCCCEEEEEEECCCCHHHH
IIEHLVEKTADQVFIPLTVGGGIRTTDDFRRILRAGADKISVNTAAIHNPDLIKQSSELY
HHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHC
GAQCVVVAIDSKRHYVNEEDLDSKQLEDKNVVKVDKNKYCWFEVFIYGGRKETGIDAIEW
CCEEEEEEEECCCCCCCCCCCCHHHCCCCCEEEECCCCEEEEEEEEECCCCCCCCHHHHH
AKTAEELGAGEILLTSMDADGTKDGYDIELTKSISTHIKLPVIASGGCGAPQHIHQVFEY
HHHHHHCCCCEEEEEECCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHC
GKADAALMASILHYREYTVGELKKYCMDKNIPMRL
CCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA