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The map label for this gene is eif2a [H]

Identifier: 150401132

GI number: 150401132

Start: 721157

End: 721945

Strand: Direct

Name: eif2a [H]

Synonym: Maeo_0703

Alternate gene names: 150401132

Gene position: 721157-721945 (Clockwise)

Preceding gene: 150401131

Following gene: 150401133

Centisome position: 45.95

GC content: 32.83

Gene sequence:

>789_bases
ATGAAAAAAGATTTTCCAGAAGAAGGAGAAATTGTTATTGGAACAGTTATTGATGTTAAACCATTTGGGGCATTCGTGGA
GCTCCTTGACTACCCTAAAAAAGAGGGTATGATACATGTATCAGAAGTATCCTCTGGATGGGTTAAAAACATCAGGGATT
TTGTTAAAAAAGGACAAAGAGTAGTAGCAAAAGTTACAAGGTCCCAAAAAAGAAAATCACAAATAGACCTTTCATTAAAA
AGAGTGACTGACCAACAGAGAAAAGTTAAAATTCAAGAATGGAAAAGACTTCAAAGAGCTGAGAAGCTTCTTGAATTTGC
AGGGAAAAAATGTGGTAAATCAATTAAAGAGGCATGGCAAGAAGTTGGGTATTTATTGGAAGAAGAATTTGGGGAAGTAT
ATGATGCCCTTGAATATCTTGTTATAGAAGGTAAAGAGGCATTAAATGAACTTGATATTTCCGAAGAGTGGGCTGATGCA
CTATATGAAATTGCAAAGGAAAACATAGAATTAACCAATGTTAAAGTAGAGGGAGTATTAACGCTAACTACTACGGATGC
CGAAGGTATTAAAAAAATTAAAAATGCCATTAAAACAGGATTAAAAGCTAACCCTTATGAGGATGTAGAAGTTGATATAA
AATACATAGGAGCTCCCCACTATAGAATTGAAATAAAATCACCAGATTATAAAAGTGGTGAAGAAGTTTTAAAAATTGTT
TCAAATTCTGCAATAGACTATATTAAAAAGAATAATGGAGACGGTAGTTTTATTAGAGAAAATGCTTAA

Upstream 100 bases:

>100_bases
AGACAAATCAAATAAATTCCTTACAGAATTTGACTTTTTGAACACACAATTATATATTTTATTATACACATACATTTATT
ATTAATTAAGCGGTGAGATT

Downstream 100 bases:

>100_bases
TTTCATTATATATAATATTATATAATGGCATATAATCTTATTCTCTATTTTAATTTATTCATTTATAGTCAATCTTCGAA
CAATTTCAGTATCGTATAAT

Product: translation initiation factor IF-2 subunit alpha

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: aIF2-alpha; eIF-2-alpha [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MKKDFPEEGEIVIGTVIDVKPFGAFVELLDYPKKEGMIHVSEVSSGWVKNIRDFVKKGQRVVAKVTRSQKRKSQIDLSLK
RVTDQQRKVKIQEWKRLQRAEKLLEFAGKKCGKSIKEAWQEVGYLLEEEFGEVYDALEYLVIEGKEALNELDISEEWADA
LYEIAKENIELTNVKVEGVLTLTTTDAEGIKKIKNAIKTGLKANPYEDVEVDIKYIGAPHYRIEIKSPDYKSGEEVLKIV
SNSAIDYIKKNNGDGSFIRENA

Sequences:

>Translated_262_residues
MKKDFPEEGEIVIGTVIDVKPFGAFVELLDYPKKEGMIHVSEVSSGWVKNIRDFVKKGQRVVAKVTRSQKRKSQIDLSLK
RVTDQQRKVKIQEWKRLQRAEKLLEFAGKKCGKSIKEAWQEVGYLLEEEFGEVYDALEYLVIEGKEALNELDISEEWADA
LYEIAKENIELTNVKVEGVLTLTTTDAEGIKKIKNAIKTGLKANPYEDVEVDIKYIGAPHYRIEIKSPDYKSGEEVLKIV
SNSAIDYIKKNNGDGSFIRENA
>Mature_262_residues
MKKDFPEEGEIVIGTVIDVKPFGAFVELLDYPKKEGMIHVSEVSSGWVKNIRDFVKKGQRVVAKVTRSQKRKSQIDLSLK
RVTDQQRKVKIQEWKRLQRAEKLLEFAGKKCGKSIKEAWQEVGYLLEEEFGEVYDALEYLVIEGKEALNELDISEEWADA
LYEIAKENIELTNVKVEGVLTLTTTDAEGIKKIKNAIKTGLKANPYEDVEVDIKYIGAPHYRIEIKSPDYKSGEEVLKIV
SNSAIDYIKKNNGDGSFIRENA

Specific function: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA [H]

COG id: COG1093

COG function: function code J; Translation initiation factor 2, alpha subunit (eIF-2alpha)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI4758256, Length=245, Percent_Identity=24.8979591836735, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17509871, Length=264, Percent_Identity=28.030303030303, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322466, Length=255, Percent_Identity=27.843137254902, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18860087, Length=262, Percent_Identity=23.6641221374046, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011488
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR022964 [H]

Pfam domain/function: PF07541 EIF_2_alpha; PF00575 S1 [H]

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 29830; Mature: 29830

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDFPEEGEIVIGTVIDVKPFGAFVELLDYPKKEGMIHVSEVSSGWVKNIRDFVKKGQR
CCCCCCCCCCEEEEEEEECCHHHHHHHHHHCCCCCCEEEEEHHCCHHHHHHHHHHHHHHH
VVAKVTRSQKRKSQIDLSLKRVTDQQRKVKIQEWKRLQRAEKLLEFAGKKCGKSIKEAWQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVGYLLEEEFGEVYDALEYLVIEGKEALNELDISEEWADALYEIAKENIELTNVKVEGVL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEEEEE
TLTTTDAEGIKKIKNAIKTGLKANPYEDVEVDIKYIGAPHYRIEIKSPDYKSGEEVLKIV
EEEECCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCHHHHHHHH
SNSAIDYIKKNNGDGSFIRENA
HHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure
MKKDFPEEGEIVIGTVIDVKPFGAFVELLDYPKKEGMIHVSEVSSGWVKNIRDFVKKGQR
CCCCCCCCCCEEEEEEEECCHHHHHHHHHHCCCCCCEEEEEHHCCHHHHHHHHHHHHHHH
VVAKVTRSQKRKSQIDLSLKRVTDQQRKVKIQEWKRLQRAEKLLEFAGKKCGKSIKEAWQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVGYLLEEEFGEVYDALEYLVIEGKEALNELDISEEWADALYEIAKENIELTNVKVEGVL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEEEEE
TLTTTDAEGIKKIKNAIKTGLKANPYEDVEVDIKYIGAPHYRIEIKSPDYKSGEEVLKIV
EEEECCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCHHHHHHHH
SNSAIDYIKKNNGDGSFIRENA
HHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]