Click here to switch to the map view.

The map label for this gene is cimA [H]

Identifier: 150401105

GI number: 150401105

Start: 691390

End: 692868

Strand: Reverse

Name: cimA [H]

Synonym: Maeo_0675

Alternate gene names: 150401105

Gene position: 692868-691390 (Counterclockwise)

Preceding gene: 150401107

Following gene: 150401101

Centisome position: 44.15

GC content: 34.62

Gene sequence:

>1479_bases
ATGATAAAAATATTGGATACAACATTAAGAGACGGAGAACAGACGCCAGGGGTTTCATTAACACCATCGGAGAAGCTTGA
AATAGCTTTAAAATTAGATGAATTAAATGTTGATATAATAGAAGCAGGTAGTGCCATAACCTCAAAAGGAGAGAGAGAAG
GCATAAAATTAATATCTCAGCAAAATTTAAATGCTGAAATATGTTCATTTGTTAGAGCTCTACCGATAGACATAGACCAT
GCCCTTGAATGTGATGTGGATAGTGTTCATTTAGTGGTTCCAACCTCGGAGCTCCACATGAAATATAAATTGAAAAAAAC
CAGCGAAGAAACATTGCAATCGGCATTGGAGGCTGTGCAATATGGTAAAGACCACGGTTTAATTGTGGAATTGTCAGCAG
AAGATGCCACAAGAAGTGATATAAACTTCTTAATAGAGTTATTTTCAAAGGGTGCAGAGATTGGAGCAGATAGGGCATGC
GTTTGCGATACTGTTGGAATATTAACACCCGAGAAATCAATAGAATTATTTAAAACATTAAGCAACAGCATAAATATACC
AATTTCAGCACATTGTCATAATGATTTTGGAATGGCTACGGCAAATACTTTATGTGCCATAAGGGGGGGAGCTAAGCAGT
GTCATCTAACAATTAATGGAATAGGGGAAAGGGCAGGTAATTCTTCACTTGAAGAGGTTGTAATGGCTTTACATAATTTA
TACAACGAAAAAACAAAAATAATCACAGAAAAATTATATGAAACTTCCAGAGTTGTTTCAAGGTTAATGAAATTACCTGT
TTCCCCAAATAAATCCCTTGTTGGAGATAATGCTTTTGCCCACGAAGCAGGAATACATGTAGATGGACTTATGAAGCACA
CCAGCACCTACGAACCATTATCTCCCGAAGAAGTCGGGAATAAAAGAAGAATTATATTGGGAAAACACAGTGGAAAATCG
GCGTTAAAATATAAGCTTAATTTAATGGAAGTATCATTAAATAATGAGGAATTTGAGGAAGTATATTCTAAAATTAAAAA
TCTTGGAGATTTGGGGAAATATATTTCAGATATCGATTTAAAAACTGTAATTAATGAAGTTAAGGGGCATAAGATTGACG
AAAAAATTATATTAAATGAATTAACAGTAGTATCTGGAAATAAAATAACTCCATTGGCGTCAATTAAATTGGACTTTGCC
GACGAAGATAATAAACATAAATCAATAATTGAAACAGCCTACGGGGTTGGACCTGTTGACGCCTCCATAAATGCACTTAG
AAAGGCTTTAAGTGGATTTGCAGATATTTCATTAATAGATTATCAAGTTTCATCCATAGGTAAGGGGACAGATGCACTAG
TTGAAGTGGTTGTTAAATTGAGAAGAGGAAATGAAGTTGTAGAGGTTAAAAAATCTCAAAGTGATATAATTAGGGCATCG
GTTGAGGCAGTTATGGAAGGAGTTAATTTATTGATTTAA

Upstream 100 bases:

>100_bases
AGTATAAATATGTTTTTATTATACTCTCATATAAATTATAAATTATAACTTGTAAATGTAAATTATAGATTATAAATATT
ATCACAATTGGGTTGATTAT

Downstream 100 bases:

>100_bases
ATTATTTTATTATTTTTTTAATATCTCATATTAATATTTTTAATTTTTTGTTCTATGTCTTACGAGCATAGCGAGTAGTA
TAGGAGCTCCTCCTGGATAA

Product: (R)-citramalate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MIKILDTTLRDGEQTPGVSLTPSEKLEIALKLDELNVDIIEAGSAITSKGEREGIKLISQQNLNAEICSFVRALPIDIDH
ALECDVDSVHLVVPTSELHMKYKLKKTSEETLQSALEAVQYGKDHGLIVELSAEDATRSDINFLIELFSKGAEIGADRAC
VCDTVGILTPEKSIELFKTLSNSINIPISAHCHNDFGMATANTLCAIRGGAKQCHLTINGIGERAGNSSLEEVVMALHNL
YNEKTKIITEKLYETSRVVSRLMKLPVSPNKSLVGDNAFAHEAGIHVDGLMKHTSTYEPLSPEEVGNKRRIILGKHSGKS
ALKYKLNLMEVSLNNEEFEEVYSKIKNLGDLGKYISDIDLKTVINEVKGHKIDEKIILNELTVVSGNKITPLASIKLDFA
DEDNKHKSIIETAYGVGPVDASINALRKALSGFADISLIDYQVSSIGKGTDALVEVVVKLRRGNEVVEVKKSQSDIIRAS
VEAVMEGVNLLI

Sequences:

>Translated_492_residues
MIKILDTTLRDGEQTPGVSLTPSEKLEIALKLDELNVDIIEAGSAITSKGEREGIKLISQQNLNAEICSFVRALPIDIDH
ALECDVDSVHLVVPTSELHMKYKLKKTSEETLQSALEAVQYGKDHGLIVELSAEDATRSDINFLIELFSKGAEIGADRAC
VCDTVGILTPEKSIELFKTLSNSINIPISAHCHNDFGMATANTLCAIRGGAKQCHLTINGIGERAGNSSLEEVVMALHNL
YNEKTKIITEKLYETSRVVSRLMKLPVSPNKSLVGDNAFAHEAGIHVDGLMKHTSTYEPLSPEEVGNKRRIILGKHSGKS
ALKYKLNLMEVSLNNEEFEEVYSKIKNLGDLGKYISDIDLKTVINEVKGHKIDEKIILNELTVVSGNKITPLASIKLDFA
DEDNKHKSIIETAYGVGPVDASINALRKALSGFADISLIDYQVSSIGKGTDALVEVVVKLRRGNEVVEVKKSQSDIIRAS
VEAVMEGVNLLI
>Mature_492_residues
MIKILDTTLRDGEQTPGVSLTPSEKLEIALKLDELNVDIIEAGSAITSKGEREGIKLISQQNLNAEICSFVRALPIDIDH
ALECDVDSVHLVVPTSELHMKYKLKKTSEETLQSALEAVQYGKDHGLIVELSAEDATRSDINFLIELFSKGAEIGADRAC
VCDTVGILTPEKSIELFKTLSNSINIPISAHCHNDFGMATANTLCAIRGGAKQCHLTINGIGERAGNSSLEEVVMALHNL
YNEKTKIITEKLYETSRVVSRLMKLPVSPNKSLVGDNAFAHEAGIHVDGLMKHTSTYEPLSPEEVGNKRRIILGKHSGKS
ALKYKLNLMEVSLNNEEFEEVYSKIKNLGDLGKYISDIDLKTVINEVKGHKIDEKIILNELTVVSGNKITPLASIKLDFA
DEDNKHKSIIETAYGVGPVDASINALRKALSGFADISLIDYQVSSIGKGTDALVEVVVKLRRGNEVVEVKKSQSDIIRAS
VEAVMEGVNLLI

Specific function: Catalyzes the condensation of pyruvate and acetyl- coenzyme A to form (R)-citramalate [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=498, Percent_Identity=33.1325301204819, Blast_Score=267, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6320019, Length=364, Percent_Identity=32.6923076923077, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6320071, Length=364, Percent_Identity=32.6923076923077, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324682, Length=559, Percent_Identity=22.8980322003578, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324225, Length=552, Percent_Identity=22.8260869565217, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.1.182 [H]

Molecular weight: Translated: 53692; Mature: 53692

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKILDTTLRDGEQTPGVSLTPSEKLEIALKLDELNVDIIEAGSAITSKGEREGIKLISQ
CEEEEECHHCCCCCCCCCCCCCCCCEEEEEEEECCCEEEEECCCHHHCCCCHHHHHHHHH
QNLNAEICSFVRALPIDIDHALECDVDSVHLVVPTSELHMKYKLKKTSEETLQSALEAVQ
CCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECHHHEEEEEECCCHHHHHHHHHHHHH
YGKDHGLIVELSAEDATRSDINFLIELFSKGAEIGADRACVCDTVGILTPEKSIELFKTL
CCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHCCCCCHHHHHHHHHH
SNSINIPISAHCHNDFGMATANTLCAIRGGAKQCHLTINGIGERAGNSSLEEVVMALHNL
HCCCCCCEEEEECCCCCCCHHHHHEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
YNEKTKIITEKLYETSRVVSRLMKLPVSPNKSLVGDNAFAHEAGIHVDGLMKHTSTYEPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCHHHHHHHCCCCCCC
SPEEVGNKRRIILGKHSGKSALKYKLNLMEVSLNNEEFEEVYSKIKNLGDLGKYISDIDL
CHHHCCCCCEEEEECCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHH
KTVINEVKGHKIDEKIILNELTVVSGNKITPLASIKLDFADEDNKHKSIIETAYGVGPVD
HHHHHHHCCCCCCHHHHHHHEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCCCC
ASINALRKALSGFADISLIDYQVSSIGKGTDALVEVVVKLRRGNEVVEVKKSQSDIIRAS
HHHHHHHHHHCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHH
VEAVMEGVNLLI
HHHHHHHHHHCC
>Mature Secondary Structure
MIKILDTTLRDGEQTPGVSLTPSEKLEIALKLDELNVDIIEAGSAITSKGEREGIKLISQ
CEEEEECHHCCCCCCCCCCCCCCCCEEEEEEEECCCEEEEECCCHHHCCCCHHHHHHHHH
QNLNAEICSFVRALPIDIDHALECDVDSVHLVVPTSELHMKYKLKKTSEETLQSALEAVQ
CCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECHHHEEEEEECCCHHHHHHHHHHHHH
YGKDHGLIVELSAEDATRSDINFLIELFSKGAEIGADRACVCDTVGILTPEKSIELFKTL
CCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHCCCCCHHHHHHHHHH
SNSINIPISAHCHNDFGMATANTLCAIRGGAKQCHLTINGIGERAGNSSLEEVVMALHNL
HCCCCCCEEEEECCCCCCCHHHHHEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
YNEKTKIITEKLYETSRVVSRLMKLPVSPNKSLVGDNAFAHEAGIHVDGLMKHTSTYEPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCHHHHHHHCCCCCCC
SPEEVGNKRRIILGKHSGKSALKYKLNLMEVSLNNEEFEEVYSKIKNLGDLGKYISDIDL
CHHHCCCCCEEEEECCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHH
KTVINEVKGHKIDEKIILNELTVVSGNKITPLASIKLDFADEDNKHKSIIETAYGVGPVD
HHHHHHHCCCCCCHHHHHHHEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCCCC
ASINALRKALSGFADISLIDYQVSSIGKGTDALVEVVVKLRRGNEVVEVKKSQSDIIRAS
HHHHHHHHHHCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHH
VEAVMEGVNLLI
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 9864346 [H]