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The map label for this gene is hacB [H]

Identifier: 150401082

GI number: 150401082

Start: 670280

End: 670777

Strand: Reverse

Name: hacB [H]

Synonym: Maeo_0652

Alternate gene names: 150401082

Gene position: 670777-670280 (Counterclockwise)

Preceding gene: 150401089

Following gene: 150401081

Centisome position: 42.74

GC content: 32.53

Gene sequence:

>498_bases
ATGATAATAAAAGGAAATATTCATTTATTTGGTGATGATATTGATACCGATGCCATAATTCCCGGGGCCTACCTTAAAAC
AACAGACCCAAAGGAGTTGGCATCTCATTGCATGGCTGGAATTGATGAAAAATTTTCAACAAAGGTAAAAGACGGCGATA
TAATTGTTGCAGGTGAAAATTTTGGCTGTGGAAGTAGTAGGGAACAGGCACCAATATCCATAAAACACACCGGAATAAAG
GCAGTAGTTGCTGAAAGTTTTGCACGGATATTTTATAGAAATTGTATTAATATAGGATTAATACCTATAACTTGCGAAGG
AATAAATGAACAAATCCAAAACCTAAAAGATGGCGACACAATAGAAATTGATTTGCAAAATGAAACAATAAAAATAAATT
CTATGATGTTAAATTGTGGAGCTCCCAAAGGGATAGAAAAAGAAATTTTAGATGCTGGTGGATTAGTACAATATACAAAA
AATAAGTTAAAAAAATAA

Upstream 100 bases:

>100_bases
AAATTCACTCAATAATTTCCAAAGGAAATTTAGAGATAGAAGCGAAGCTTCTCTATATTCAAATCAAAGATTTTAACCTC
AAAGATTATATGGTGATACT

Downstream 100 bases:

>100_bases
GTTAAGAAATAGAAAACAAAATTTAAAATTTAAAAAAAGTGATATAATGACAGGAAGATTGGCAATTTTAGATTATGATA
GATGTCAGCCCCGAAGATGC

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: HACN; (R)-homocitrate dehydratase; Homoaconitate hydratase [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MIIKGNIHLFGDDIDTDAIIPGAYLKTTDPKELASHCMAGIDEKFSTKVKDGDIIVAGENFGCGSSREQAPISIKHTGIK
AVVAESFARIFYRNCINIGLIPITCEGINEQIQNLKDGDTIEIDLQNETIKINSMMLNCGAPKGIEKEILDAGGLVQYTK
NKLKK

Sequences:

>Translated_165_residues
MIIKGNIHLFGDDIDTDAIIPGAYLKTTDPKELASHCMAGIDEKFSTKVKDGDIIVAGENFGCGSSREQAPISIKHTGIK
AVVAESFARIFYRNCINIGLIPITCEGINEQIQNLKDGDTIEIDLQNETIKINSMMLNCGAPKGIEKEILDAGGLVQYTK
NKLKK
>Mature_165_residues
MIIKGNIHLFGDDIDTDAIIPGAYLKTTDPKELASHCMAGIDEKFSTKVKDGDIIVAGENFGCGSSREQAPISIKHTGIK
AVVAESFARIFYRNCINIGLIPITCEGINEQIQNLKDGDTIEIDLQNETIKINSMMLNCGAPKGIEKEILDAGGLVQYTK
NKLKK

Specific function: Hydro-lyase with broad substrate specificity for cis- unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4- tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4- tricarboxyla

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=136, Percent_Identity=34.5588235294118, Blast_Score=75, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320440, Length=175, Percent_Identity=36.5714285714286, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=136, Percent_Identity=34.5588235294118, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.36 [H]

Molecular weight: Translated: 17954; Mature: 17954

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKGNIHLFGDDIDTDAIIPGAYLKTTDPKELASHCMAGIDEKFSTKVKDGDIIVAGEN
CEEECCEEEECCCCCCCEECCCCEEECCCHHHHHHHHHHCCCCHHCCCCCCCCEEEECCC
FGCGSSREQAPISIKHTGIKAVVAESFARIFYRNCINIGLIPITCEGINEQIQNLKDGDT
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHCCCCCCE
IEIDLQNETIKINSMMLNCGAPKGIEKEILDAGGLVQYTKNKLKK
EEEEECCCEEEEEEEEEECCCCCCCHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MIIKGNIHLFGDDIDTDAIIPGAYLKTTDPKELASHCMAGIDEKFSTKVKDGDIIVAGEN
CEEECCEEEECCCCCCCEECCCCEEECCCHHHHHHHHHHCCCCHHCCCCCCCCEEEECCC
FGCGSSREQAPISIKHTGIKAVVAESFARIFYRNCINIGLIPITCEGINEQIQNLKDGDT
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHCCCCCCE
IEIDLQNETIKINSMMLNCGAPKGIEKEILDAGGLVQYTKNKLKK
EEEEECCCEEEEEEEEEECCCCCCCHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]