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The map label for this gene is metG

Identifier: 150401049

GI number: 150401049

Start: 634435

End: 636453

Strand: Reverse

Name: metG

Synonym: Maeo_0619

Alternate gene names: 150401049

Gene position: 636453-634435 (Counterclockwise)

Preceding gene: 150401050

Following gene: 150401048

Centisome position: 40.55

GC content: 32.39

Gene sequence:

>2019_bases
ATGAATAATAAATACCTTTTAACTACGGCATTGGCATATACAAATGGACCACTTCATTTGGGACATGCTAGAAGCACATA
CATACCTGCGGACATAATAAAAAGATATTTGAAATTAAAGGGCAATGATGTAATTCATGTTGGGGGAACAGATAATCATG
GTGTTCCCATCACACTTACAGCGGAAAAGGAAGGAGTAAAACCCGAGGATATTGTAAATAAATATCATAATGAAATAAAA
AGAGATTTGGACAATTTAAGCGTAGAGTTTGATAGCTATGGAAAAACCCACAGCGATACCCATATACAAAATGCTCAGGA
ATTTTACAAAAAACTTAAAGAAAATGGATATATTTATGAAAAAGAAATAGAACAATTTTACTGTCCTGATTGCGATAAAT
TTTTAGCAGATAGGTATGTTGAAGGAAGTTGCCCATTTTGCGAGGGTGAGGCAAGAGGAGACCACTGTGAAGTTTGCGGA
AGGCATCTTGAACCTACCGAGTTGGTAAATCCTTACTGTGTAATTTGTAATGCTACTCCCCAGCTTAAAAAAACCACCCA
CTATTTCTTTAAATTAAGTGCTTTATCTGATAATTTGGACAATTATGTAAAAAATTCAAATATGCCCGACCATATAAAAA
ATATGGCATACAGTTGGATTAGGGAGCTCCATGATTGGGATATATCCCGAGGTATAAAATGGGGAGTGCCAATTCCAGAT
GACGAAAACCAAGTTATGTATGTTTGGTTGGAAGCTCCTATTGGATATATCTCATTTACAAAGATGATGGGCGATATTTG
GAAAGAATACTGGTTAAAAAATAATGATAATAACACAGAAAACCAGAACACAAAAATATGGCACTTCATAGGTAAGGATA
TTACTGTGCATCATGCCGTATTTTGGCCAGGGATGTTGCTTGGGCATGGTGAATACAACCTACCAAATTCAATATTTAGC
GGTGGCTATTTAACTCTTGAAGGAAAAAAGATGAGCACCAGTAAAAAGTGGGTTGTTTGGGTCGATGATTTTATTAAATA
CTTTGATTCCGATTATTTGAGATACTTTTTAATGGCAAATGCTCCATTAAATAGAGATTGTGATTTTTCATTTGATGAAT
TCCAAAAAAGGATAAATACGGAGCTCATTGCAATCATTGCAAACTTCACACATAGGGCTTTAGTATTCTCCCATAGAAAA
TTTGGAGCCTTACCTGTTATAGATAAACCAGAAGAGGAATTGAATAAAGAAGATTTGGCTTTACTTGATAAATGCTCGGA
AACCATTGAAAAATATAATAAAAATATGATGGATTGTAATTTAAAAGATGCACTGGTTGATATAATACATTTAGCAAAAG
AAGGAAATAACTACTTCCAAAAAATGGAGCCTTGGACAGTTGATGATGAAAATAGATTAAAACAAATAATGTTTGTTTGC
GGAGTAATTACAAAGCATATTGCTTATTTATTGTATCCATTTATGCCAAATAAAACAAATGAATTATTGGATTTAATGAA
TGAAGAGATGGATTTAGAAATAAGAGGAAATGAATTAAAAAAGCCAATCGTTGTATTTTCAAAAATTGAAAATGATACAA
TTAAAATGGTAAAGGAAAAACTATTAAAATCAGAAAATAGTAAAAATACAAAGAATAAAAAGAAAAATACAAAGAAAAAT
AAAAACGGTGAAAAAATGGAATTAATTGGTATTGATGATTTTGCTAAAATTGAATTAAAAGTTGCTCAAATATTGGAAGC
TGAGGAAGTTCCTAAGTCCAAAAAACTTTTAAAATTAATTGTAGATATTGGAGACGAAAAAAGGCAGGTTGTAGCTGGAA
TTAAAGGACATTACACGCCAGAAGAATTAATTGGTAAAAAAATTGTATTAGTATGTAATTTAAAACCTGCTAAATTATGT
GGCGTTGAATCCCAAGGAATGGTATTGGCTGCTGGTGATGAGGAAGTGGCTTTATTATCGCCTGAAAAAGACCTTCCAGT
AGGAAGCACAATTTGTTAA

Upstream 100 bases:

>100_bases
TTAAGATGCTTATAATTTTTACATTTTTACAATCTTTTATAATTCAATATTTTATAATATCATGCAATATAATAATGATA
ATAAATAACAGAGGTAAAAA

Downstream 100 bases:

>100_bases
TATTTTATGGGGGCTTTAAAATTTTTTCTTGGAAAATTTTAAAGTCATAAAACTTGCGAAGCAAGTTTGAAATTCCAAGG
AGTTCCTTTTATTTTATTTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MNNKYLLTTALAYTNGPLHLGHARSTYIPADIIKRYLKLKGNDVIHVGGTDNHGVPITLTAEKEGVKPEDIVNKYHNEIK
RDLDNLSVEFDSYGKTHSDTHIQNAQEFYKKLKENGYIYEKEIEQFYCPDCDKFLADRYVEGSCPFCEGEARGDHCEVCG
RHLEPTELVNPYCVICNATPQLKKTTHYFFKLSALSDNLDNYVKNSNMPDHIKNMAYSWIRELHDWDISRGIKWGVPIPD
DENQVMYVWLEAPIGYISFTKMMGDIWKEYWLKNNDNNTENQNTKIWHFIGKDITVHHAVFWPGMLLGHGEYNLPNSIFS
GGYLTLEGKKMSTSKKWVVWVDDFIKYFDSDYLRYFLMANAPLNRDCDFSFDEFQKRINTELIAIIANFTHRALVFSHRK
FGALPVIDKPEEELNKEDLALLDKCSETIEKYNKNMMDCNLKDALVDIIHLAKEGNNYFQKMEPWTVDDENRLKQIMFVC
GVITKHIAYLLYPFMPNKTNELLDLMNEEMDLEIRGNELKKPIVVFSKIENDTIKMVKEKLLKSENSKNTKNKKKNTKKN
KNGEKMELIGIDDFAKIELKVAQILEAEEVPKSKKLLKLIVDIGDEKRQVVAGIKGHYTPEELIGKKIVLVCNLKPAKLC
GVESQGMVLAAGDEEVALLSPEKDLPVGSTIC

Sequences:

>Translated_672_residues
MNNKYLLTTALAYTNGPLHLGHARSTYIPADIIKRYLKLKGNDVIHVGGTDNHGVPITLTAEKEGVKPEDIVNKYHNEIK
RDLDNLSVEFDSYGKTHSDTHIQNAQEFYKKLKENGYIYEKEIEQFYCPDCDKFLADRYVEGSCPFCEGEARGDHCEVCG
RHLEPTELVNPYCVICNATPQLKKTTHYFFKLSALSDNLDNYVKNSNMPDHIKNMAYSWIRELHDWDISRGIKWGVPIPD
DENQVMYVWLEAPIGYISFTKMMGDIWKEYWLKNNDNNTENQNTKIWHFIGKDITVHHAVFWPGMLLGHGEYNLPNSIFS
GGYLTLEGKKMSTSKKWVVWVDDFIKYFDSDYLRYFLMANAPLNRDCDFSFDEFQKRINTELIAIIANFTHRALVFSHRK
FGALPVIDKPEEELNKEDLALLDKCSETIEKYNKNMMDCNLKDALVDIIHLAKEGNNYFQKMEPWTVDDENRLKQIMFVC
GVITKHIAYLLYPFMPNKTNELLDLMNEEMDLEIRGNELKKPIVVFSKIENDTIKMVKEKLLKSENSKNTKNKKKNTKKN
KNGEKMELIGIDDFAKIELKVAQILEAEEVPKSKKLLKLIVDIGDEKRQVVAGIKGHYTPEELIGKKIVLVCNLKPAKLC
GVESQGMVLAAGDEEVALLSPEKDLPVGSTIC
>Mature_672_residues
MNNKYLLTTALAYTNGPLHLGHARSTYIPADIIKRYLKLKGNDVIHVGGTDNHGVPITLTAEKEGVKPEDIVNKYHNEIK
RDLDNLSVEFDSYGKTHSDTHIQNAQEFYKKLKENGYIYEKEIEQFYCPDCDKFLADRYVEGSCPFCEGEARGDHCEVCG
RHLEPTELVNPYCVICNATPQLKKTTHYFFKLSALSDNLDNYVKNSNMPDHIKNMAYSWIRELHDWDISRGIKWGVPIPD
DENQVMYVWLEAPIGYISFTKMMGDIWKEYWLKNNDNNTENQNTKIWHFIGKDITVHHAVFWPGMLLGHGEYNLPNSIFS
GGYLTLEGKKMSTSKKWVVWVDDFIKYFDSDYLRYFLMANAPLNRDCDFSFDEFQKRINTELIAIIANFTHRALVFSHRK
FGALPVIDKPEEELNKEDLALLDKCSETIEKYNKNMMDCNLKDALVDIIHLAKEGNNYFQKMEPWTVDDENRLKQIMFVC
GVITKHIAYLLYPFMPNKTNELLDLMNEEMDLEIRGNELKKPIVVFSKIENDTIKMVKEKLLKSENSKNTKNKKKNTKKN
KNGEKMELIGIDDFAKIELKVAQILEAEEVPKSKKLLKLIVDIGDEKRQVVAGIKGHYTPEELIGKKIVLVCNLKPAKLC
GVESQGMVLAAGDEEVALLSPEKDLPVGSTIC

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=649, Percent_Identity=27.8890600924499, Blast_Score=274, Evalue=2e-73,
Organism=Homo sapiens, GI24308436, Length=527, Percent_Identity=24.2884250474383, Blast_Score=169, Evalue=1e-41,
Organism=Homo sapiens, GI4507947, Length=133, Percent_Identity=33.8345864661654, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1788432, Length=686, Percent_Identity=32.5072886297376, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1789455, Length=107, Percent_Identity=39.2523364485981, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786861, Length=220, Percent_Identity=25.9090909090909, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540794, Length=609, Percent_Identity=31.3628899835796, Blast_Score=312, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI212645096, Length=503, Percent_Identity=26.2425447316103, Blast_Score=171, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71992781, Length=396, Percent_Identity=26.7676767676768, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321704, Length=561, Percent_Identity=31.729055258467, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6321610, Length=551, Percent_Identity=26.3157894736842, Blast_Score=136, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6323414, Length=250, Percent_Identity=27.2, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24655311, Length=564, Percent_Identity=31.5602836879433, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI21358483, Length=531, Percent_Identity=26.1770244821092, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_META3 (A6UUN1)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324815.1
- ProteinModelPortal:   A6UUN1
- STRING:   A6UUN1
- GeneID:   5326396
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0619
- eggNOG:   arNOG04371
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- BioCyc:   MAEO419665:MAEO_0619-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 77318; Mature: 77318

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD; PS00307 LECTIN_LEGUME_BETA

Important sites: BINDING 333-333

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKYLLTTALAYTNGPLHLGHARSTYIPADIIKRYLKLKGNDVIHVGGTDNHGVPITLT
CCCCEEEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCEEEEEE
AEKEGVKPEDIVNKYHNEIKRDLDNLSVEFDSYGKTHSDTHIQNAQEFYKKLKENGYIYE
CCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEE
KEIEQFYCPDCDKFLADRYVEGSCPFCEGEARGDHCEVCGRHLEPTELVNPYCVICNATP
EHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHCCCEEEEECCCC
QLKKTTHYFFKLSALSDNLDNYVKNSNMPDHIKNMAYSWIRELHDWDISRGIKWGVPIPD
CHHHCEEEEEEEEHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DENQVMYVWLEAPIGYISFTKMMGDIWKEYWLKNNDNNTENQNTKIWHFIGKDITVHHAV
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEHH
FWPGMLLGHGEYNLPNSIFSGGYLTLEGKKMSTSKKWVVWVDDFIKYFDSDYLRYFLMAN
HCCCEEEECCCCCCCCHHCCCCEEEEECCEECCCCCEEEEHHHHHHHHCCHHHEEHHEEC
APLNRDCDFSFDEFQKRINTELIAIIANFTHRALVFSHRKFGALPVIDKPEEELNKEDLA
CCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHCCHHHHH
LLDKCSETIEKYNKNMMDCNLKDALVDIIHLAKEGNNYFQKMEPWTVDDENRLKQIMFVC
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHH
GVITKHIAYLLYPFMPNKTNELLDLMNEEMDLEIRGNELKKPIVVFSKIENDTIKMVKEK
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEECCCCCCCCEEEEEECCCHHHHHHHHH
LLKSENSKNTKNKKKNTKKNKNGEKMELIGIDDFAKIELKVAQILEAEEVPKSKKLLKLI
HHHCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
VDIGDEKRQVVAGIKGHYTPEELIGKKIVLVCNLKPAKLCGVESQGMVLAAGDEEVALLS
HHCCCCHHHHHHCCCCCCCHHHHCCCEEEEEECCCCHHHCCCCCCCEEEEECCCCEEEEC
PEKDLPVGSTIC
CCCCCCCCCCCC
>Mature Secondary Structure
MNNKYLLTTALAYTNGPLHLGHARSTYIPADIIKRYLKLKGNDVIHVGGTDNHGVPITLT
CCCCEEEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCEEEEEE
AEKEGVKPEDIVNKYHNEIKRDLDNLSVEFDSYGKTHSDTHIQNAQEFYKKLKENGYIYE
CCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEE
KEIEQFYCPDCDKFLADRYVEGSCPFCEGEARGDHCEVCGRHLEPTELVNPYCVICNATP
EHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHCCCEEEEECCCC
QLKKTTHYFFKLSALSDNLDNYVKNSNMPDHIKNMAYSWIRELHDWDISRGIKWGVPIPD
CHHHCEEEEEEEEHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DENQVMYVWLEAPIGYISFTKMMGDIWKEYWLKNNDNNTENQNTKIWHFIGKDITVHHAV
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEHH
FWPGMLLGHGEYNLPNSIFSGGYLTLEGKKMSTSKKWVVWVDDFIKYFDSDYLRYFLMAN
HCCCEEEECCCCCCCCHHCCCCEEEEECCEECCCCCEEEEHHHHHHHHCCHHHEEHHEEC
APLNRDCDFSFDEFQKRINTELIAIIANFTHRALVFSHRKFGALPVIDKPEEELNKEDLA
CCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHCCHHHHH
LLDKCSETIEKYNKNMMDCNLKDALVDIIHLAKEGNNYFQKMEPWTVDDENRLKQIMFVC
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHH
GVITKHIAYLLYPFMPNKTNELLDLMNEEMDLEIRGNELKKPIVVFSKIENDTIKMVKEK
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEECCCCCCCCEEEEEECCCHHHHHHHHH
LLKSENSKNTKNKKKNTKKNKNGEKMELIGIDDFAKIELKVAQILEAEEVPKSKKLLKLI
HHHCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
VDIGDEKRQVVAGIKGHYTPEELIGKKIVLVCNLKPAKLCGVESQGMVLAAGDEEVALLS
HHCCCCHHHHHHCCCCCCCHHHHCCCEEEEEECCCCHHHCCCCCCCEEEEECCCCEEEEC
PEKDLPVGSTIC
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA