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The map label for this gene is mdh [H]

Identifier: 150401041

GI number: 150401041

Start: 625333

End: 626274

Strand: Reverse

Name: mdh [H]

Synonym: Maeo_0610

Alternate gene names: 150401041

Gene position: 626274-625333 (Counterclockwise)

Preceding gene: 150401048

Following gene: 150401039

Centisome position: 39.9

GC content: 33.76

Gene sequence:

>942_bases
ATGGAAATATCAATAATAGGGGCGTCAGGAAAAATAGGAACTGCAATATCTGTATTACTTGCAAAGGAAAAATATGTAAG
GCATATTAATTTAATATCACGGGAAAAATCACTGGATAAGTTAAAGGGACTTAAATTGGATATATACGATGCCATAGCCG
CAGAAGAATTAGATGTTGAAATCTCTGTTCATGATGAGAAAGATCTTTCGGTAGTTTGCAATAGTAAAATTACAATAATT
CCAGCCGGAGCTCCGAGGACAGGAGATATGACAAGGTTGGATTTAGCTAAAAAAAATGCGGGAATTGTAAAAAGATATGC
AAAAGATATAGGTAAAACTTGCGATACAAAATTATTTATGATTACAAATCCTGTTGATGTGATGACACATAAGGCACTTA
TTGAAAGTGGATATGATAAAAGCCAAGTTTTTGGTTTGGGGACTCATCTTGACTCCATGAGGTTTAAGGTGGCAATTGCA
AAATATTTTAATGCACATATCGGAGATGTTAGAACCAGAATAATTGGGGAGCATGGGGATAGTATGGTTCCATTAATTAG
CTCCACGGCGATAGGGGGGATACCAATAACAAGATTGCCAGAATATAAGGATTTTCCATATAATGAAGTGGTGGATTTTG
TTAAAAATGGGGGAAAACGGATTATACAATTAAAAGGAGGTTCTGAGTATGGTCCAGCATCTGCGGTGTTAAATGTGGTT
AGATGCATTGCAAATGATAATAAAAAATATTTAACATTATCCACATACCTTGACGGTGAATTAGATGGTATAAAAGATGT
ATGTATTGGTGTTCCCGTAGTAATTGGAAAAAAAGGAATAGAAAGAATTGTTCCTATTGAATTGGATGAAAAGGAATTTA
ACGACTTTAAAAAATCTGTTGAAATTGTAAAAAGATATTGGAATGAAGTTAAAGGTATTTAA

Upstream 100 bases:

>100_bases
AATAAGTATTACAACAATTATATTATATATATTTTATGGCACATAGATAATTACAAAATCAAAATCATTAAAACAAGAAT
TAAAAAAGAGAGGTGATAAT

Downstream 100 bases:

>100_bases
TAAAAAATATTTAATAAAAAAAATATTTAAAAAGGACATCTAATATTTTTACCTGCATACTTAGGATATCCTATTTCTTC
TTCAATCCTAATTAATTGAT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MEISIIGASGKIGTAISVLLAKEKYVRHINLISREKSLDKLKGLKLDIYDAIAAEELDVEISVHDEKDLSVVCNSKITII
PAGAPRTGDMTRLDLAKKNAGIVKRYAKDIGKTCDTKLFMITNPVDVMTHKALIESGYDKSQVFGLGTHLDSMRFKVAIA
KYFNAHIGDVRTRIIGEHGDSMVPLISSTAIGGIPITRLPEYKDFPYNEVVDFVKNGGKRIIQLKGGSEYGPASAVLNVV
RCIANDNKKYLTLSTYLDGELDGIKDVCIGVPVVIGKKGIERIVPIELDEKEFNDFKKSVEIVKRYWNEVKGI

Sequences:

>Translated_313_residues
MEISIIGASGKIGTAISVLLAKEKYVRHINLISREKSLDKLKGLKLDIYDAIAAEELDVEISVHDEKDLSVVCNSKITII
PAGAPRTGDMTRLDLAKKNAGIVKRYAKDIGKTCDTKLFMITNPVDVMTHKALIESGYDKSQVFGLGTHLDSMRFKVAIA
KYFNAHIGDVRTRIIGEHGDSMVPLISSTAIGGIPITRLPEYKDFPYNEVVDFVKNGGKRIIQLKGGSEYGPASAVLNVV
RCIANDNKKYLTLSTYLDGELDGIKDVCIGVPVVIGKKGIERIVPIELDEKEFNDFKKSVEIVKRYWNEVKGI
>Mature_313_residues
MEISIIGASGKIGTAISVLLAKEKYVRHINLISREKSLDKLKGLKLDIYDAIAAEELDVEISVHDEKDLSVVCNSKITII
PAGAPRTGDMTRLDLAKKNAGIVKRYAKDIGKTCDTKLFMITNPVDVMTHKALIESGYDKSQVFGLGTHLDSMRFKVAIA
KYFNAHIGDVRTRIIGEHGDSMVPLISSTAIGGIPITRLPEYKDFPYNEVVDFVKNGGKRIIQLKGGSEYGPASAVLNVV
RCIANDNKKYLTLSTYLDGELDGIKDVCIGVPVVIGKKGIERIVPIELDEKEFNDFKKSVEIVKRYWNEVKGI

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=321, Percent_Identity=30.2180685358255, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI221136809, Length=321, Percent_Identity=30.2180685358255, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI9257228, Length=315, Percent_Identity=31.4285714285714, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4504973, Length=315, Percent_Identity=31.4285714285714, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI15082234, Length=321, Percent_Identity=28.9719626168224, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI5031857, Length=309, Percent_Identity=30.42071197411, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI260099723, Length=309, Percent_Identity=30.42071197411, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI291575128, Length=306, Percent_Identity=27.1241830065359, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI4557032, Length=306, Percent_Identity=27.1241830065359, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI260099727, Length=199, Percent_Identity=31.1557788944724, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI260099725, Length=199, Percent_Identity=31.1557788944724, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI207028494, Length=190, Percent_Identity=31.0526315789474, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI103472011, Length=250, Percent_Identity=22.4, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI21735621, Length=316, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1789632, Length=307, Percent_Identity=27.0358306188925, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535107, Length=269, Percent_Identity=31.2267657992565, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17554310, Length=301, Percent_Identity=27.2425249169435, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6322765, Length=304, Percent_Identity=27.3026315789474, Blast_Score=73, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324446, Length=260, Percent_Identity=27.6923076923077, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320125, Length=300, Percent_Identity=27.3333333333333, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136226, Length=313, Percent_Identity=28.1150159744409, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI45550422, Length=319, Percent_Identity=29.7805642633229, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24647881, Length=321, Percent_Identity=23.98753894081, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 34497; Mature: 34497

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEISIIGASGKIGTAISVLLAKEKYVRHINLISREKSLDKLKGLKLDIYDAIAAEELDVE
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHEEEE
ISVHDEKDLSVVCNSKITIIPAGAPRTGDMTRLDLAKKNAGIVKRYAKDIGKTCDTKLFM
EEEECCCCCEEEECCEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCEEEE
ITNPVDVMTHKALIESGYDKSQVFGLGTHLDSMRFKVAIAKYFNAHIGDVRTRIIGEHGD
EECCHHHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SMVPLISSTAIGGIPITRLPEYKDFPYNEVVDFVKNGGKRIIQLKGGSEYGPASAVLNVV
CCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHH
RCIANDNKKYLTLSTYLDGELDGIKDVCIGVPVVIGKKGIERIVPIELDEKEFNDFKKSV
HHHHCCCCCEEEEEEECCCCCCCHHHHHHCCCCEECCCCCCCCCCEEECHHHHHHHHHHH
EIVKRYWNEVKGI
HHHHHHHHHHCCC
>Mature Secondary Structure
MEISIIGASGKIGTAISVLLAKEKYVRHINLISREKSLDKLKGLKLDIYDAIAAEELDVE
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHEEEE
ISVHDEKDLSVVCNSKITIIPAGAPRTGDMTRLDLAKKNAGIVKRYAKDIGKTCDTKLFM
EEEECCCCCEEEECCEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCEEEE
ITNPVDVMTHKALIESGYDKSQVFGLGTHLDSMRFKVAIAKYFNAHIGDVRTRIIGEHGD
EECCHHHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SMVPLISSTAIGGIPITRLPEYKDFPYNEVVDFVKNGGKRIIQLKGGSEYGPASAVLNVV
CCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHH
RCIANDNKKYLTLSTYLDGELDGIKDVCIGVPVVIGKKGIERIVPIELDEKEFNDFKKSV
HHHHCCCCCEEEEEEECCCCCCCHHHHHHCCCCEECCCCCCCCCCEEECHHHHHHHHHHH
EIVKRYWNEVKGI
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA