Click here to switch to the map view.

The map label for this gene is eno [H]

Identifier: 150401040

GI number: 150401040

Start: 624017

End: 625306

Strand: Direct

Name: eno [H]

Synonym: Maeo_0609

Alternate gene names: 150401040

Gene position: 624017-625306 (Clockwise)

Preceding gene: 150401036

Following gene: 150401042

Centisome position: 39.76

GC content: 36.98

Gene sequence:

>1290_bases
TTGTTAAAAAATATGGATAATTCTTTTGATATTTTAGATATTAAAGCAAGAGAAGTTATAGACTCAAGAGGAAACCCTAC
CGTAGAGGTTGAGGTATTAACCGCAGGTGGATATGGTAGTGCCATAGTTCCAAGTGGTGCATCAACGGGAACCCATGAAG
CCGTAGAATTGAGAGACAAAAGCCAGAGATTTGGTGGAAAAGGAGTTTTAGTTGCTGTGGATAATGTCAATAGTATTATT
GCACCTGAATTAATCGGATTTGATTCAAGAACGCAAAGGGAAATTGATACCTATATGATAGAATTGGATAGAACGGCAAA
TAAAGGAAAATTAGGAGCTAATGCAATACTTGCCGTATCAATGGCAGTGGCAAAAGCAGCAGCAAGCACGGCATCACTTC
CATTATACAAATACATTGGTGGATGTAATTCATATATTATGCCTGTTCCAATGATGAATGTTATTAATGGAGGGGAGCAT
GCGGGAAATGCCCTTGAATTCCAAGAATTTATGATTATGCCCGTTGGAGCAGATTCAATCAGCGAAGCCATAAGAATGTG
TGCTGAAACATACCACACACTAAAAAAAGTGATAGTTAGCAAATATGGGAAAAATGCTTCAAATGTTGGAGATGAAGGAG
GATTTGCACCACCGGTAAGCGATATAAGGGAAACACTTGATTTATTAACCGATGCCGTTAAAATGGCAGGTTATGAAGAT
GAAATTACATTTGCTTTGGATTGTGCGGCAAGTGAATTTTACAATAAAGATGATAACAAATATATTGTAAAGGGAGCTCC
TTTATCTTCCGACCAACTTATTTCATTATATAAAGAAATATGTGATGAATATCCAGTAATATCCATAGAAGACCCACTTG
ATGAGGAGGACTTTGAAGGATTTGCCTCACTTACCAAAGAATTAAAAGGAGTTCAAATAGTTGGGGACGATTTATTTGTT
ACAAACACAGAGAGATTGAAAAAGGGAATAGAAATGGGAGCTGGAAATGCCTTATTATTAAAGGTAAATCAGATAGGAAC
ACTATCAGAATCAATAGATGCGGCAAATCTTGCTTTTAGAAATGGATATGGTGTGGTTGTATCCCACAGAAGTGGGGAAA
GTGAGGATAACACAATTGCAGATATTTCCGTTGCTTTAAATGCGGGACAGATTAAAACGGGAGCTCCTGCGAGAGGGGAA
AGGACAGCCAAATATAATCAATTAATTAGGATTGAAGAAGAAATAGGATATCCTAAGTATGCAGGTAAAAATATTAGATG
TCCTTTTTAA

Upstream 100 bases:

>100_bases
CTAAATATTATATTGCTATTCTCTAAATTATTACAATTATTGTTATTATGTACGCTTTTTTTTATTTTATACACCCAATA
TTTAAAACCGAGGTGAAATA

Downstream 100 bases:

>100_bases
ATATTTTTTTTATTAAATATTTTTTATTAAATACCTTTAACTTCATTCCAATATCTTTTTACAATTTCAACAGATTTTTT
AAAGTCGTTAAATTCCTTTT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MLKNMDNSFDILDIKAREVIDSRGNPTVEVEVLTAGGYGSAIVPSGASTGTHEAVELRDKSQRFGGKGVLVAVDNVNSII
APELIGFDSRTQREIDTYMIELDRTANKGKLGANAILAVSMAVAKAAASTASLPLYKYIGGCNSYIMPVPMMNVINGGEH
AGNALEFQEFMIMPVGADSISEAIRMCAETYHTLKKVIVSKYGKNASNVGDEGGFAPPVSDIRETLDLLTDAVKMAGYED
EITFALDCAASEFYNKDDNKYIVKGAPLSSDQLISLYKEICDEYPVISIEDPLDEEDFEGFASLTKELKGVQIVGDDLFV
TNTERLKKGIEMGAGNALLLKVNQIGTLSESIDAANLAFRNGYGVVVSHRSGESEDNTIADISVALNAGQIKTGAPARGE
RTAKYNQLIRIEEEIGYPKYAGKNIRCPF

Sequences:

>Translated_429_residues
MLKNMDNSFDILDIKAREVIDSRGNPTVEVEVLTAGGYGSAIVPSGASTGTHEAVELRDKSQRFGGKGVLVAVDNVNSII
APELIGFDSRTQREIDTYMIELDRTANKGKLGANAILAVSMAVAKAAASTASLPLYKYIGGCNSYIMPVPMMNVINGGEH
AGNALEFQEFMIMPVGADSISEAIRMCAETYHTLKKVIVSKYGKNASNVGDEGGFAPPVSDIRETLDLLTDAVKMAGYED
EITFALDCAASEFYNKDDNKYIVKGAPLSSDQLISLYKEICDEYPVISIEDPLDEEDFEGFASLTKELKGVQIVGDDLFV
TNTERLKKGIEMGAGNALLLKVNQIGTLSESIDAANLAFRNGYGVVVSHRSGESEDNTIADISVALNAGQIKTGAPARGE
RTAKYNQLIRIEEEIGYPKYAGKNIRCPF
>Mature_429_residues
MLKNMDNSFDILDIKAREVIDSRGNPTVEVEVLTAGGYGSAIVPSGASTGTHEAVELRDKSQRFGGKGVLVAVDNVNSII
APELIGFDSRTQREIDTYMIELDRTANKGKLGANAILAVSMAVAKAAASTASLPLYKYIGGCNSYIMPVPMMNVINGGEH
AGNALEFQEFMIMPVGADSISEAIRMCAETYHTLKKVIVSKYGKNASNVGDEGGFAPPVSDIRETLDLLTDAVKMAGYED
EITFALDCAASEFYNKDDNKYIVKGAPLSSDQLISLYKEICDEYPVISIEDPLDEEDFEGFASLTKELKGVQIVGDDLFV
TNTERLKKGIEMGAGNALLLKVNQIGTLSESIDAANLAFRNGYGVVVSHRSGESEDNTIADISVALNAGQIKTGAPARGE
RTAKYNQLIRIEEEIGYPKYAGKNIRCPF

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=55.787037037037, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI4503571, Length=437, Percent_Identity=53.5469107551487, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=54.7785547785548, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=54.7785547785548, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897479, Length=426, Percent_Identity=51.1737089201878, Blast_Score=380, Evalue=1e-106,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=26.865671641791, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=26.865671641791, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=26.865671641791, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1789141, Length=418, Percent_Identity=55.7416267942584, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71995829, Length=433, Percent_Identity=54.7344110854504, Blast_Score=443, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17536383, Length=433, Percent_Identity=54.7344110854504, Blast_Score=443, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI32563855, Length=197, Percent_Identity=50.761421319797, Blast_Score=189, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321693, Length=430, Percent_Identity=52.7906976744186, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6323985, Length=422, Percent_Identity=51.4218009478673, Blast_Score=401, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324974, Length=422, Percent_Identity=51.1848341232227, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324969, Length=422, Percent_Identity=51.1848341232227, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321968, Length=430, Percent_Identity=51.6279069767442, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580918, Length=437, Percent_Identity=53.5469107551487, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24580916, Length=437, Percent_Identity=53.5469107551487, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24580920, Length=437, Percent_Identity=53.5469107551487, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24580914, Length=437, Percent_Identity=53.5469107551487, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=53.953488372093, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=53.953488372093, Blast_Score=424, Evalue=1e-119,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46230; Mature: 46230

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNMDNSFDILDIKAREVIDSRGNPTVEVEVLTAGGYGSAIVPSGASTGTHEAVELRDK
CCCCCCCCCCEEEEHHHHHHHCCCCCEEEEEEEEECCCCCEECCCCCCCCCCHHHHHHHH
SQRFGGKGVLVAVDNVNSIIAPELIGFDSRTQREIDTYMIELDRTANKGKLGANAILAVS
HHHCCCCEEEEEECCCCCHHCHHHHCCCCCHHHHHEEEEEEEECCCCCCCCCCHHHHHHH
MAVAKAAASTASLPLYKYIGGCNSYIMPVPMMNVINGGEHAGNALEFQEFMIMPVGADSI
HHHHHHHHHHCCCCHHHHHCCCCCEECCCCHHHHHCCCCCCCCCEEEEEEEEEECCHHHH
SEAIRMCAETYHTLKKVIVSKYGKNASNVGDEGGFAPPVSDIRETLDLLTDAVKMAGYED
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
EITFALDCAASEFYNKDDNKYIVKGAPLSSDQLISLYKEICDEYPVISIEDPLDEEDFEG
CEEEEEEEHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHH
FASLTKELKGVQIVGDDLFVTNTERLKKGIEMGAGNALLLKVNQIGTLSESIDAANLAFR
HHHHHHHHCCEEEECCCEEEECHHHHHHHHHCCCCCEEEEEECCCCCHHHHCCHHHEEEE
NGYGVVVSHRSGESEDNTIADISVALNAGQIKTGAPARGERTAKYNQLIRIEEEIGYPKY
CCCEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHEEEHHHCCCCCC
AGKNIRCPF
CCCCCCCCC
>Mature Secondary Structure
MLKNMDNSFDILDIKAREVIDSRGNPTVEVEVLTAGGYGSAIVPSGASTGTHEAVELRDK
CCCCCCCCCCEEEEHHHHHHHCCCCCEEEEEEEEECCCCCEECCCCCCCCCCHHHHHHHH
SQRFGGKGVLVAVDNVNSIIAPELIGFDSRTQREIDTYMIELDRTANKGKLGANAILAVS
HHHCCCCEEEEEECCCCCHHCHHHHCCCCCHHHHHEEEEEEEECCCCCCCCCCHHHHHHH
MAVAKAAASTASLPLYKYIGGCNSYIMPVPMMNVINGGEHAGNALEFQEFMIMPVGADSI
HHHHHHHHHHCCCCHHHHHCCCCCEECCCCHHHHHCCCCCCCCCEEEEEEEEEECCHHHH
SEAIRMCAETYHTLKKVIVSKYGKNASNVGDEGGFAPPVSDIRETLDLLTDAVKMAGYED
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
EITFALDCAASEFYNKDDNKYIVKGAPLSSDQLISLYKEICDEYPVISIEDPLDEEDFEG
CEEEEEEEHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHH
FASLTKELKGVQIVGDDLFVTNTERLKKGIEMGAGNALLLKVNQIGTLSESIDAANLAFR
HHHHHHHHCCEEEECCCEEEECHHHHHHHHHCCCCCEEEEEECCCCCHHHHCCHHHEEEE
NGYGVVVSHRSGESEDNTIADISVALNAGQIKTGAPARGERTAKYNQLIRIEEEIGYPKY
CCCEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHEEEHHHCCCCCC
AGKNIRCPF
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA