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The map label for this gene is pykF [C]

Identifier: 150400975

GI number: 150400975

Start: 558990

End: 560342

Strand: Direct

Name: pykF [C]

Synonym: Maeo_0544

Alternate gene names: 150400975

Gene position: 558990-560342 (Clockwise)

Preceding gene: 150400972

Following gene: 150400976

Centisome position: 35.62

GC content: 30.38

Gene sequence:

>1353_bases
ATGAAAAATAACATATTTAACCGTAAAACTAAAATATTAATAACAATGGGCCCATCTATCGAACGAGTGTTGGAAAAATC
AGCAAATATAATTGATGGAGTTAGGTTTAACATGTCCCATGCTAAGATTGAGGAGATGGATAAATATATGGAAATATTGA
ATAAAAACAATATTGCAAAATTAATGGATTTAAAGGGAAATAAATTACGAATTAAAACAACCAACATATCAAGTATAAAA
GCGGGAGAAACAGTAATAATTGGTAAAGATGTAATATTGTCCCACCACCCAGTAAATGAGGTGGAAATTGGGCATTTTAT
ACTAATAAATGATGGAAAGGTTAAATTGAAAATAGATAATATTAAAGATAATTTAATATATGCAACGGCGATAGTTGGGG
GAGAAATAAAACAAGGCAATGGAATAAATACTCCGGATTCGAGTTTAAAAATGCCAATAATCGCCATTGAAGATATTGAA
CATATAAAATATGCGATTAAACATGATTTTGAATATATTGCACTATCATTTGTTAGAAATAAAAACGATATAATCAATTT
AAAAAATATTTTACAAGAGTATAATGGAAATATACCAATAATAGCTAAAATAGAGACAAAAGAAGGTTTAAAAAACATAG
AGGAAATATCAATATATGCAGACGGAATTATGGTTGCTAGGGGGGATTTAGGTGTTGAAATTCCTATTGAAAATATTCCT
GTAAAACAGAAGGAAATAATAAGGACGGCAAATAAACACGGAAAAATAGTAATAACTGCAACTCAAATGCTTGATTCAAT
GGTAAATAATCCATACCCAACGAGAGCGGAATTGACAGACATTGCAAATGCTATATATGATGGAACAGACTGTATAATGC
TATCAAATGAAACATCAATAGGAAAATACCCTATAGAAGCTATTAAGGTAATGGATAATGTTGCAAAAATATCTGAAAAA
GATATCGATAAATTTGGGAGAAAAACAAAATTGGGGAGTTGCTCAACTTCAATAGGCATAGCTTATGCCACATATGCACT
TTTTGAGCAATTACGCCCCAAAATAATATTAACGCCCACATGGTCGGGAAGTAGTGCCAAACTTATATCCAAATTTAAGC
CTTCAATTCCAATAATTGCACCTACGCCCAATATAAAAACATTTAGAAAATTAAGACTCGTATGGGGCGTTACTCCTGTT
CTTTCAGATGAAGTTCATAGTATTGACGACATTTTAAATTTATCAAAAGAAATAGCTAAAAAATATATTGATACTGGAAC
ATATATTATAACTTTGGGGCATCCAATTGGCATGAAAAAGACCAATTTAATTAAAGTGGAAACAATTGAATAG

Upstream 100 bases:

>100_bases
CTCACTTTTATATAAATTAATTTAGGAAACGATATATAAAATAAACATGCATAATAATGCATGTCTAAAAAATATTATTG
CCACGAGGGGGAGGGAGAAA

Downstream 100 bases:

>100_bases
AAAAATAATAATGATAATAATGCAATAATAAAAATAACATAGTTAAGTAATATGCTAATAATAATATAACTAAACTATTT
TAAAGGCTGATGCAATGATA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKNNIFNRKTKILITMGPSIERVLEKSANIIDGVRFNMSHAKIEEMDKYMEILNKNNIAKLMDLKGNKLRIKTTNISSIK
AGETVIIGKDVILSHHPVNEVEIGHFILINDGKVKLKIDNIKDNLIYATAIVGGEIKQGNGINTPDSSLKMPIIAIEDIE
HIKYAIKHDFEYIALSFVRNKNDIINLKNILQEYNGNIPIIAKIETKEGLKNIEEISIYADGIMVARGDLGVEIPIENIP
VKQKEIIRTANKHGKIVITATQMLDSMVNNPYPTRAELTDIANAIYDGTDCIMLSNETSIGKYPIEAIKVMDNVAKISEK
DIDKFGRKTKLGSCSTSIGIAYATYALFEQLRPKIILTPTWSGSSAKLISKFKPSIPIIAPTPNIKTFRKLRLVWGVTPV
LSDEVHSIDDILNLSKEIAKKYIDTGTYIITLGHPIGMKKTNLIKVETIE

Sequences:

>Translated_450_residues
MKNNIFNRKTKILITMGPSIERVLEKSANIIDGVRFNMSHAKIEEMDKYMEILNKNNIAKLMDLKGNKLRIKTTNISSIK
AGETVIIGKDVILSHHPVNEVEIGHFILINDGKVKLKIDNIKDNLIYATAIVGGEIKQGNGINTPDSSLKMPIIAIEDIE
HIKYAIKHDFEYIALSFVRNKNDIINLKNILQEYNGNIPIIAKIETKEGLKNIEEISIYADGIMVARGDLGVEIPIENIP
VKQKEIIRTANKHGKIVITATQMLDSMVNNPYPTRAELTDIANAIYDGTDCIMLSNETSIGKYPIEAIKVMDNVAKISEK
DIDKFGRKTKLGSCSTSIGIAYATYALFEQLRPKIILTPTWSGSSAKLISKFKPSIPIIAPTPNIKTFRKLRLVWGVTPV
LSDEVHSIDDILNLSKEIAKKYIDTGTYIITLGHPIGMKKTNLIKVETIE
>Mature_450_residues
MKNNIFNRKTKILITMGPSIERVLEKSANIIDGVRFNMSHAKIEEMDKYMEILNKNNIAKLMDLKGNKLRIKTTNISSIK
AGETVIIGKDVILSHHPVNEVEIGHFILINDGKVKLKIDNIKDNLIYATAIVGGEIKQGNGINTPDSSLKMPIIAIEDIE
HIKYAIKHDFEYIALSFVRNKNDIINLKNILQEYNGNIPIIAKIETKEGLKNIEEISIYADGIMVARGDLGVEIPIENIP
VKQKEIIRTANKHGKIVITATQMLDSMVNNPYPTRAELTDIANAIYDGTDCIMLSNETSIGKYPIEAIKVMDNVAKISEK
DIDKFGRKTKLGSCSTSIGIAYATYALFEQLRPKIILTPTWSGSSAKLISKFKPSIPIIAPTPNIKTFRKLRLVWGVTPV
LSDEVHSIDDILNLSKEIAKKYIDTGTYIITLGHPIGMKKTNLIKVETIE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=32.719836400818, Blast_Score=232, Evalue=7e-61,
Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=32.9938900203666, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=32.9938900203666, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI10835121, Length=492, Percent_Identity=30.2845528455285, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI32967597, Length=492, Percent_Identity=30.2845528455285, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=30.6896551724138, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=30.6896551724138, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=25.1046025104602, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=25.1046025104602, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI310128738, Length=111, Percent_Identity=28.8288288288288, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1787965, Length=447, Percent_Identity=34.8993288590604, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI1788160, Length=487, Percent_Identity=32.2381930184805, Blast_Score=208, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI17544584, Length=429, Percent_Identity=33.3333333333333, Blast_Score=221, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=31.0838445807771, Blast_Score=204, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI71984406, Length=489, Percent_Identity=31.0838445807771, Blast_Score=204, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17506829, Length=492, Percent_Identity=31.0975609756098, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=31.0838445807771, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6319279, Length=483, Percent_Identity=31.8840579710145, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6324923, Length=484, Percent_Identity=31.6115702479339, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=31.7813765182186, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=31.7813765182186, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24648966, Length=313, Percent_Identity=35.1437699680511, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24581235, Length=434, Percent_Identity=30.6451612903226, Blast_Score=198, Evalue=6e-51,
Organism=Drosophila melanogaster, GI24646914, Length=281, Percent_Identity=33.8078291814947, Blast_Score=142, Evalue=3e-34,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50201; Mature: 50201

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNIFNRKTKILITMGPSIERVLEKSANIIDGVRFNMSHAKIEEMDKYMEILNKNNIAK
CCCCCCCCCEEEEEEECCCHHHHHHHCCCHHCCEEECCCHHHHHHHHHHHHHHCCCCCEE
LMDLKGNKLRIKTTNISSIKAGETVIIGKDVILSHHPVNEVEIGHFILINDGKVKLKIDN
HHCCCCCEEEEEECCCCCCCCCCEEEEECCEEEECCCCCCEECCEEEEEECCEEEEEEEC
IKDNLIYATAIVGGEIKQGNGINTPDSSLKMPIIAIEDIEHIKYAIKHDFEYIALSFVRN
CCCCEEEEEEEECCEEECCCCCCCCCCCCEEEEEEECCHHHHHHHHHCCHHEEEEEEECC
KNDIINLKNILQEYNGNIPIIAKIETKEGLKNIEEISIYADGIMVARGDLGVEIPIENIP
CCCEEHHHHHHHHHCCCCCEEEEECCHHHHCCHHHEEEEECCEEEEECCCCEEEECCCCC
VKQKEIIRTANKHGKIVITATQMLDSMVNNPYPTRAELTDIANAIYDGTDCIMLSNETSI
CCHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
GKYPIEAIKVMDNVAKISEKDIDKFGRKTKLGSCSTSIGIAYATYALFEQLRPKIILTPT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEC
WSGSSAKLISKFKPSIPIIAPTPNIKTFRKLRLVWGVTPVLSDEVHSIDDILNLSKEIAK
CCCCCHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KYIDTGTYIITLGHPIGMKKTNLIKVETIE
HHHCCCCEEEEECCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MKNNIFNRKTKILITMGPSIERVLEKSANIIDGVRFNMSHAKIEEMDKYMEILNKNNIAK
CCCCCCCCCEEEEEEECCCHHHHHHHCCCHHCCEEECCCHHHHHHHHHHHHHHCCCCCEE
LMDLKGNKLRIKTTNISSIKAGETVIIGKDVILSHHPVNEVEIGHFILINDGKVKLKIDN
HHCCCCCEEEEEECCCCCCCCCCEEEEECCEEEECCCCCCEECCEEEEEECCEEEEEEEC
IKDNLIYATAIVGGEIKQGNGINTPDSSLKMPIIAIEDIEHIKYAIKHDFEYIALSFVRN
CCCCEEEEEEEECCEEECCCCCCCCCCCCEEEEEEECCHHHHHHHHHCCHHEEEEEEECC
KNDIINLKNILQEYNGNIPIIAKIETKEGLKNIEEISIYADGIMVARGDLGVEIPIENIP
CCCEEHHHHHHHHHCCCCCEEEEECCHHHHCCHHHEEEEECCEEEEECCCCEEEECCCCC
VKQKEIIRTANKHGKIVITATQMLDSMVNNPYPTRAELTDIANAIYDGTDCIMLSNETSI
CCHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
GKYPIEAIKVMDNVAKISEKDIDKFGRKTKLGSCSTSIGIAYATYALFEQLRPKIILTPT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEC
WSGSSAKLISKFKPSIPIIAPTPNIKTFRKLRLVWGVTPVLSDEVHSIDDILNLSKEIAK
CCCCCHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KYIDTGTYIITLGHPIGMKKTNLIKVETIE
HHHCCCCEEEEECCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]