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The map label for this gene is phoA [H]

Identifier: 150400972

GI number: 150400972

Start: 554965

End: 556485

Strand: Direct

Name: phoA [H]

Synonym: Maeo_0541

Alternate gene names: 150400972

Gene position: 554965-556485 (Clockwise)

Preceding gene: 150400971

Following gene: 150400975

Centisome position: 35.36

GC content: 35.37

Gene sequence:

>1521_bases
TTGAAAAAAGCAATAGGCATATTAGGTGTAATATTCACAATATTCATATTGCTGGCATCTCAGGCGAGTGCAGCAAAAAG
TAATGCAGAATCAGCATCAGCATTTAAGTCAAATGGAGTTAAAAATGTAATAATCTTGATAGGGGATGGAATGGGATTCA
GTCAGTTGAAACTAACTAAGCAGTGTTATGGTCATTTAAGTATGGAAGATATTCCATCCACAGGTTTTGAGCTCACCGAC
TCATTAAGTGGTGAGGTTACAGATTCAGCAGCAGCTGGAACAGCAATTGCCACAGGATTTAAAACTTACAATGGGATGAT
TTCAACAATAAAAAAAGGCGAAATTAAAAATGTGACAACATTATTGGAATTGGCAGAACTATGCAATAAATCCACAGGAT
TGGTATCAACAGCAAGAATTACCCATGCAACTCCTGCGGTATTTGCTTCCCATGTTGAAGATAGGGACATGGAAAAAGAA
ATATCTAAACAGCTAATAGAGCACAAAGTAAATGTAATGTTTGGTGGCGGTAAAAAAGAATTTGACAGCGATACCTTGAA
TATGGCAAAAAATTATGGATATAATATTGTTTATGATAAAAAAGGATTAGAATCAGCAAATGGAAATTATATATTGGGAT
TGTTCTCAGATAGTCATATACCTTATGTATTGGATAGAGATGAAAATACACCAGGATTATTAGATATGACTAAAAAAGCA
GTAGATTTATTAGAAAAAGACAATGATGGATTCTTTTTAATGATCGAAGCTGGAAGAATTGACCATGCAAGCCATGCCAA
TGATATTGCGTCAGTGGTGGCTGAAACAAAAGAATTTGATGATGTTGTAAATTACTGCTTAGATTATGCAAGGAAAAACA
AAGATACTTTGGTTATTGTTCTTGCCGACCATGAAACTGGTGGACTGGCTGTGGGGACAGGATACGGTAATCCAGTGAAT
GAAGAAAAAATATTAAACATAAAAGCAAGCACCGACAAAATGGCAAAAGAAATAAAAAGCGGAAAGGACCCGAAAGAAGT
TTTAAAAGAATACGCAGGAATAACATTAACCGATGATGAAGTTAAATTGATAAATGATGCTAGGAGCTCCGATAACAAGT
ATGCCCTTGGAAATACCATAGCAGAAATCATTGATGAAAAGGTTGGAGTTGGATTTGTATCCCACAAACACACCGGAGCT
CCTGTGCCATTAATGGTGTATGGAAAAGGTTCTGAGAATTTTAGGGGATTTTTACACCATGTAGATACAAGTAAGGAGAC
GGCAAAATTGATGTTGTTTGGAAATACCAACAAATACATAAAGAACTACGGCGTAAGCAGTTTAAAAGGAGATGCAAATG
GTGATTTTGTAATCAACAAAGATGACGCATACGTTACATTAATGTCCTATGTTGGGGAAGAAGTTGATACACAGAATGAA
GAAAAATTAGATATGGATAATAATGGATTGATTGATTACATGGATGTTGCCATTATAATGAATAAGGCGGAATCCTACTA
A

Upstream 100 bases:

>100_bases
TGTATATCATTTAGTTCATATTTTATGAAATAAAATTCAATAATAGAAAATTTTATATACTATATTATGAACAAATAATT
ATGTATATGGAGGTGAAATC

Downstream 100 bases:

>100_bases
GTAGTTTAAATTCAAACCATCTATTATTGTTATTATTTTATTTTTTCCTTGTATTTAAGAGATTTTGATTATTTTAGTTT
ATGGTTATATTGTATTTTGA

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKKAIGILGVIFTIFILLASQASAAKSNAESASAFKSNGVKNVIILIGDGMGFSQLKLTKQCYGHLSMEDIPSTGFELTD
SLSGEVTDSAAAGTAIATGFKTYNGMISTIKKGEIKNVTTLLELAELCNKSTGLVSTARITHATPAVFASHVEDRDMEKE
ISKQLIEHKVNVMFGGGKKEFDSDTLNMAKNYGYNIVYDKKGLESANGNYILGLFSDSHIPYVLDRDENTPGLLDMTKKA
VDLLEKDNDGFFLMIEAGRIDHASHANDIASVVAETKEFDDVVNYCLDYARKNKDTLVIVLADHETGGLAVGTGYGNPVN
EEKILNIKASTDKMAKEIKSGKDPKEVLKEYAGITLTDDEVKLINDARSSDNKYALGNTIAEIIDEKVGVGFVSHKHTGA
PVPLMVYGKGSENFRGFLHHVDTSKETAKLMLFGNTNKYIKNYGVSSLKGDANGDFVINKDDAYVTLMSYVGEEVDTQNE
EKLDMDNNGLIDYMDVAIIMNKAESY

Sequences:

>Translated_506_residues
MKKAIGILGVIFTIFILLASQASAAKSNAESASAFKSNGVKNVIILIGDGMGFSQLKLTKQCYGHLSMEDIPSTGFELTD
SLSGEVTDSAAAGTAIATGFKTYNGMISTIKKGEIKNVTTLLELAELCNKSTGLVSTARITHATPAVFASHVEDRDMEKE
ISKQLIEHKVNVMFGGGKKEFDSDTLNMAKNYGYNIVYDKKGLESANGNYILGLFSDSHIPYVLDRDENTPGLLDMTKKA
VDLLEKDNDGFFLMIEAGRIDHASHANDIASVVAETKEFDDVVNYCLDYARKNKDTLVIVLADHETGGLAVGTGYGNPVN
EEKILNIKASTDKMAKEIKSGKDPKEVLKEYAGITLTDDEVKLINDARSSDNKYALGNTIAEIIDEKVGVGFVSHKHTGA
PVPLMVYGKGSENFRGFLHHVDTSKETAKLMLFGNTNKYIKNYGVSSLKGDANGDFVINKDDAYVTLMSYVGEEVDTQNE
EKLDMDNNGLIDYMDVAIIMNKAESY
>Mature_506_residues
MKKAIGILGVIFTIFILLASQASAAKSNAESASAFKSNGVKNVIILIGDGMGFSQLKLTKQCYGHLSMEDIPSTGFELTD
SLSGEVTDSAAAGTAIATGFKTYNGMISTIKKGEIKNVTTLLELAELCNKSTGLVSTARITHATPAVFASHVEDRDMEKE
ISKQLIEHKVNVMFGGGKKEFDSDTLNMAKNYGYNIVYDKKGLESANGNYILGLFSDSHIPYVLDRDENTPGLLDMTKKA
VDLLEKDNDGFFLMIEAGRIDHASHANDIASVVAETKEFDDVVNYCLDYARKNKDTLVIVLADHETGGLAVGTGYGNPVN
EEKILNIKASTDKMAKEIKSGKDPKEVLKEYAGITLTDDEVKLINDARSSDNKYALGNTIAEIIDEKVGVGFVSHKHTGA
PVPLMVYGKGSENFRGFLHHVDTSKETAKLMLFGNTNKYIKNYGVSSLKGDANGDFVINKDDAYVTLMSYVGEEVDTQNE
EKLDMDNNGLIDYMDVAIIMNKAESY

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=349, Percent_Identity=28.080229226361, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI157266296, Length=387, Percent_Identity=27.906976744186, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI94721246, Length=387, Percent_Identity=27.6485788113695, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI294660770, Length=325, Percent_Identity=27.6923076923077, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI157266292, Length=327, Percent_Identity=27.5229357798165, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI294660772, Length=287, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=7e-24,
Organism=Escherichia coli, GI48994877, Length=333, Percent_Identity=33.033033033033, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320689, Length=497, Percent_Identity=31.1871227364185, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI18859923, Length=292, Percent_Identity=33.5616438356164, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24651554, Length=334, Percent_Identity=30.8383233532934, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI19921912, Length=356, Percent_Identity=30.6179775280899, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI21355149, Length=355, Percent_Identity=30.7042253521127, Blast_Score=145, Evalue=9e-35,
Organism=Drosophila melanogaster, GI21355151, Length=321, Percent_Identity=32.7102803738318, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI21355981, Length=326, Percent_Identity=31.2883435582822, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24585213, Length=338, Percent_Identity=29.8816568047337, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24651556, Length=306, Percent_Identity=30.3921568627451, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21358067, Length=326, Percent_Identity=28.8343558282209, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI85725200, Length=333, Percent_Identity=27.6276276276276, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI85815833, Length=333, Percent_Identity=27.6276276276276, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24657835, Length=452, Percent_Identity=25, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24657842, Length=352, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24657827, Length=346, Percent_Identity=27.4566473988439, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 55033; Mature: 55033

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00018 EF_HAND_1 ; PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAIGILGVIFTIFILLASQASAAKSNAESASAFKSNGVKNVIILIGDGMGFSQLKLTK
CCCHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHH
QCYGHLSMEDIPSTGFELTDSLSGEVTDSAAAGTAIATGFKTYNGMISTIKKGEIKNVTT
HHHCCCCHHHCCCCCCEECCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
LLELAELCNKSTGLVSTARITHATPAVFASHVEDRDMEKEISKQLIEHKVNVMFGGGKKE
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCEEEEECCCCCC
FDSDTLNMAKNYGYNIVYDKKGLESANGNYILGLFSDSHIPYVLDRDENTPGLLDMTKKA
CCCHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHH
VDLLEKDNDGFFLMIEAGRIDHASHANDIASVVAETKEFDDVVNYCLDYARKNKDTLVIV
HHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LADHETGGLAVGTGYGNPVNEEKILNIKASTDKMAKEIKSGKDPKEVLKEYAGITLTDDE
EEECCCCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHCCCEECCCC
VKLINDARSSDNKYALGNTIAEIIDEKVGVGFVSHKHTGAPVPLMVYGKGSENFRGFLHH
HHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEECCCCHHHHHHHH
VDTSKETAKLMLFGNTNKYIKNYGVSSLKGDANGDFVINKDDAYVTLMSYVGEEVDTQNE
CCCCCCCEEEEEEECCCHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHCHHCCCCCC
EKLDMDNNGLIDYMDVAIIMNKAESY
CCCCCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKAIGILGVIFTIFILLASQASAAKSNAESASAFKSNGVKNVIILIGDGMGFSQLKLTK
CCCHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHH
QCYGHLSMEDIPSTGFELTDSLSGEVTDSAAAGTAIATGFKTYNGMISTIKKGEIKNVTT
HHHCCCCHHHCCCCCCEECCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
LLELAELCNKSTGLVSTARITHATPAVFASHVEDRDMEKEISKQLIEHKVNVMFGGGKKE
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCEEEEECCCCCC
FDSDTLNMAKNYGYNIVYDKKGLESANGNYILGLFSDSHIPYVLDRDENTPGLLDMTKKA
CCCHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHH
VDLLEKDNDGFFLMIEAGRIDHASHANDIASVVAETKEFDDVVNYCLDYARKNKDTLVIV
HHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LADHETGGLAVGTGYGNPVNEEKILNIKASTDKMAKEIKSGKDPKEVLKEYAGITLTDDE
EEECCCCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHCCCEECCCC
VKLINDARSSDNKYALGNTIAEIIDEKVGVGFVSHKHTGAPVPLMVYGKGSENFRGFLHH
HHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEECCCCHHHHHHHH
VDTSKETAKLMLFGNTNKYIKNYGVSSLKGDANGDFVINKDDAYVTLMSYVGEEVDTQNE
CCCCCCCEEEEEEECCCHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHCHHCCCCCC
EKLDMDNNGLIDYMDVAIIMNKAESY
CCCCCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]