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The map label for this gene is nifE [H]

Identifier: 150400939

GI number: 150400939

Start: 527694

End: 529097

Strand: Reverse

Name: nifE [H]

Synonym: Maeo_0507

Alternate gene names: 150400939

Gene position: 529097-527694 (Counterclockwise)

Preceding gene: 150400940

Following gene: 150400938

Centisome position: 33.71

GC content: 34.76

Gene sequence:

>1404_bases
ATGCAGTATAAAGAACAGAAAATAAGAATTTGCTCAAACCTTCCACTGCATAGGCAAAGCGGTGTTCCTGGGAAGGTTTG
TGGAGCTTTAAGGATTGCAAACCAAATAAAAGGATGTGTCCCAATACTCCATTCACCAGTAGGTTGTGCATTCCAAAGGA
AGATAAACACATTTGCACCTTATGAGATAACCTACAATTTACCTTGCACAAATTTAACAGAGGTTGATACAGTTTATGGC
GGTCATGAGAAATTGGTAGAAAAGGTTTTGGAGGTTAATAAAAAATACCATCCTGAGCTCATATTAATTATATCAACATG
TGCCCCAGATTTGATTGGGGAGGATTACGATATTACATTGGATGAAGTCAGGGATATGATTGATGCAAAGATTATATACG
ACACAGGGAATGCAAAGAGAGCTCCCGTTGGCATGCAAAGTGCATTATATTCATTAGCCACTCAGGTAATGGAGGAACAG
GATAAAATTGAAAAGAGTTTAAATATTTGCAAACTTTCATACCATAGAACAGATTCAGTTGATGAATTTGTAGATATTTT
GGAAGAGATGGGAGCTCATGTTAATGGAGTTTATTTCAACAACAACACCATTGAAGATATAAAAAACATTCCAAGGGCTG
AGCTCAATTTGGTAGATTTTCCAGCAGATTGGGTATTATATGCCGAGAAGAAATTTGGGACAAAATATCTTATGCCGCCA
CGACTAAAAATAGAGGACTCACTAACGACATTAAATGGGTTTGCCAAATATTTGTATGCTATTGCCGGAGCTCTGGACTT
GGATACTGATGTAATTGAAAAAAGAAAAAAAGAAGCAGATGAAAAACTTGAAAAATATAGGAAAAAATTAAAAGGTAAAA
AAATAGCAAGTGGATTTTCAGTTCATGGAGGGGCAATGCAAACATTGATTGAAGACGTAGGAATGGAATGCCCTGTTTTA
ATATTAAAAACTAGAAGAATGAATTTAATGATGAAAGATGATGCTGTTAAACACATATCCAATTCAATGACAAGATTTAT
TGAAAATCATCAAGGATATGCTCCAGAAGTATTGATAAATCCAACTACTGAGGAAGAAATAAAGGCTATGAAGGAACATG
GTATTGAATTGTGCGTAGGCGGAATGGAAAATAACATGTTTGAATATTTAAGAAACGGCATAAAATTATTCGATATGAGG
CGGATGATGCAAATATCAAAAATGGGCTTCAACAATTCTGTAAATATAGCAAGGGAAACCTATAAAATTGTCTCCAAAGA
ACCAAAATCACACTTGTTATTGGGAAATATAGAGTATTCAATGAGAAATCCAAGTTTATCAAACTATTGGGCAGATATTG
TTGATATATTCCAAACATGCTGGCATTGTGATTGTAAGGACTAA

Upstream 100 bases:

>100_bases
AAATATCTTTAAATAATACTCCACTTGCTGGATTTAGGGGAGCTCCTACAATTATTGAAAGAATTATAAATGAAATTACA
AAGGCATAATAGGTGGCAAT

Downstream 100 bases:

>100_bases
TCATAATGAGATTAATGTAATTATATAAAATAAGAATTAAGGTGATAATGTGAAACAAATAGCATTTTATGGAAAAGGAG
GAATTGGTAAATCTACAACA

Product: oxidoreductase/nitrogenase component 1

Products: oxidized ferredoxin; NH3; ADP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MQYKEQKIRICSNLPLHRQSGVPGKVCGALRIANQIKGCVPILHSPVGCAFQRKINTFAPYEITYNLPCTNLTEVDTVYG
GHEKLVEKVLEVNKKYHPELILIISTCAPDLIGEDYDITLDEVRDMIDAKIIYDTGNAKRAPVGMQSALYSLATQVMEEQ
DKIEKSLNICKLSYHRTDSVDEFVDILEEMGAHVNGVYFNNNTIEDIKNIPRAELNLVDFPADWVLYAEKKFGTKYLMPP
RLKIEDSLTTLNGFAKYLYAIAGALDLDTDVIEKRKKEADEKLEKYRKKLKGKKIASGFSVHGGAMQTLIEDVGMECPVL
ILKTRRMNLMMKDDAVKHISNSMTRFIENHQGYAPEVLINPTTEEEIKAMKEHGIELCVGGMENNMFEYLRNGIKLFDMR
RMMQISKMGFNNSVNIARETYKIVSKEPKSHLLLGNIEYSMRNPSLSNYWADIVDIFQTCWHCDCKD

Sequences:

>Translated_467_residues
MQYKEQKIRICSNLPLHRQSGVPGKVCGALRIANQIKGCVPILHSPVGCAFQRKINTFAPYEITYNLPCTNLTEVDTVYG
GHEKLVEKVLEVNKKYHPELILIISTCAPDLIGEDYDITLDEVRDMIDAKIIYDTGNAKRAPVGMQSALYSLATQVMEEQ
DKIEKSLNICKLSYHRTDSVDEFVDILEEMGAHVNGVYFNNNTIEDIKNIPRAELNLVDFPADWVLYAEKKFGTKYLMPP
RLKIEDSLTTLNGFAKYLYAIAGALDLDTDVIEKRKKEADEKLEKYRKKLKGKKIASGFSVHGGAMQTLIEDVGMECPVL
ILKTRRMNLMMKDDAVKHISNSMTRFIENHQGYAPEVLINPTTEEEIKAMKEHGIELCVGGMENNMFEYLRNGIKLFDMR
RMMQISKMGFNNSVNIARETYKIVSKEPKSHLLLGNIEYSMRNPSLSNYWADIVDIFQTCWHCDCKD
>Mature_467_residues
MQYKEQKIRICSNLPLHRQSGVPGKVCGALRIANQIKGCVPILHSPVGCAFQRKINTFAPYEITYNLPCTNLTEVDTVYG
GHEKLVEKVLEVNKKYHPELILIISTCAPDLIGEDYDITLDEVRDMIDAKIIYDTGNAKRAPVGMQSALYSLATQVMEEQ
DKIEKSLNICKLSYHRTDSVDEFVDILEEMGAHVNGVYFNNNTIEDIKNIPRAELNLVDFPADWVLYAEKKFGTKYLMPP
RLKIEDSLTTLNGFAKYLYAIAGALDLDTDVIEKRKKEADEKLEKYRKKLKGKKIASGFSVHGGAMQTLIEDVGMECPVL
ILKTRRMNLMMKDDAVKHISNSMTRFIENHQGYAPEVLINPTTEEEIKAMKEHGIELCVGGMENNMFEYLRNGIKLFDMR
RMMQISKMGFNNSVNIARETYKIVSKEPKSHLLLGNIEYSMRNPSLSNYWADIVDIFQTCWHCDCKD

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: 1.18.6.1

Molecular weight: Translated: 53234; Mature: 53234

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYKEQKIRICSNLPLHRQSGVPGKVCGALRIANQIKGCVPILHSPVGCAFQRKINTFAP
CCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
YEITYNLPCTNLTEVDTVYGGHEKLVEKVLEVNKKYHPELILIISTCAPDLIGEDYDITL
EEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHCCCCCCEEH
DEVRDMIDAKIIYDTGNAKRAPVGMQSALYSLATQVMEEQDKIEKSLNICKLSYHRTDSV
HHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCH
DEFVDILEEMGAHVNGVYFNNNTIEDIKNIPRAELNLVDFPADWVLYAEKKFGTKYLMPP
HHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCCCEEEECCCCEEEEEEHHCCCEECCCC
RLKIEDSLTTLNGFAKYLYAIAGALDLDTDVIEKRKKEADEKLEKYRKKLKGKKIASGFS
CCEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCC
VHGGAMQTLIEDVGMECPVLILKTRRMNLMMKDDAVKHISNSMTRFIENHQGYAPEVLIN
CCCHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
PTTEEEIKAMKEHGIELCVGGMENNMFEYLRNGIKLFDMRRMMQISKMGFNNSVNIARET
CCCHHHHHHHHHCCCEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
YKIVSKEPKSHLLLGNIEYSMRNPSLSNYWADIVDIFQTCWHCDCKD
HHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQYKEQKIRICSNLPLHRQSGVPGKVCGALRIANQIKGCVPILHSPVGCAFQRKINTFAP
CCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
YEITYNLPCTNLTEVDTVYGGHEKLVEKVLEVNKKYHPELILIISTCAPDLIGEDYDITL
EEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHCCCCCCEEH
DEVRDMIDAKIIYDTGNAKRAPVGMQSALYSLATQVMEEQDKIEKSLNICKLSYHRTDSV
HHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCH
DEFVDILEEMGAHVNGVYFNNNTIEDIKNIPRAELNLVDFPADWVLYAEKKFGTKYLMPP
HHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCCCEEEECCCCEEEEEEHHCCCEECCCC
RLKIEDSLTTLNGFAKYLYAIAGALDLDTDVIEKRKKEADEKLEKYRKKLKGKKIASGFS
CCEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCC
VHGGAMQTLIEDVGMECPVLILKTRRMNLMMKDDAVKHISNSMTRFIENHQGYAPEVLIN
CCCHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
PTTEEEIKAMKEHGIELCVGGMENNMFEYLRNGIKLFDMRRMMQISKMGFNNSVNIARET
CCCHHHHHHHHHCCCEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
YKIVSKEPKSHLLLGNIEYSMRNPSLSNYWADIVDIFQTCWHCDCKD
HHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; H+; N2; ATP

Specific reaction: 3 reduced ferredoxin + 6 H+ + N2 + n ATP = 3 oxidized ferredoxin + 2 NH3 + n ADP + n phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2726466 [H]