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The map label for this gene is lacA [C]

Identifier: 150400858

GI number: 150400858

Start: 444653

End: 445081

Strand: Direct

Name: lacA [C]

Synonym: Maeo_0426

Alternate gene names: 150400858

Gene position: 444653-445081 (Clockwise)

Preceding gene: 150400857

Following gene: 150400859

Centisome position: 28.33

GC content: 29.14

Gene sequence:

>429_bases
ATGAGATTTAAAAATTGGAAATTTCCAAAAATCGAGCATAATAAACCAACAGAATATAACTGGGTTGTTCAATACCCTGA
AAATTTAGAGCTCGGAAAATACACAGATATTGGAGCATTTACATATATAAATTCAAAGTATGGGGTTAAAATTGATGAGT
ATGTGCAAATAGGTTCTCATTGTTCTATATACTCTGTTTCAACAATAGATAATAAAAAAGGAAAAGTGATAATAAATAAA
AATGCAAGAATCGGAAGCCATAGTGTAATTATGCCAAATGTTGAAATTGGAGAAAATTCTATTATCGGAGCATTTAGTTT
TGTAAATAAAAACATTCCACCGAATACATTAGCTTATGGCGTTCCAGTGAAACTTATTAGGAAACTAACTGATGAAGAAA
TTAAAAAGTTATTGAAGGAGATAGAATGA

Upstream 100 bases:

>100_bases
CTGTGGATGCAATAGAAACATTGACGAAAAAGAGAGGTTCAGAGATTGGGATTAAATATGCTGAAAGTTTTTACTTAATA
AGAGAAATTCAGGGATAACA

Downstream 100 bases:

>100_bases
AAATTCCACTATTTAAAATTTACTGGGATAATGAAGATATTAAATCTGTTGAAAAAATTATAAAATCGGGCATGTTTTGG
AGCTCCGGTAATGAGATTGA

Product: hexapaptide repeat-containing transferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MRFKNWKFPKIEHNKPTEYNWVVQYPENLELGKYTDIGAFTYINSKYGVKIDEYVQIGSHCSIYSVSTIDNKKGKVIINK
NARIGSHSVIMPNVEIGENSIIGAFSFVNKNIPPNTLAYGVPVKLIRKLTDEEIKKLLKEIE

Sequences:

>Translated_142_residues
MRFKNWKFPKIEHNKPTEYNWVVQYPENLELGKYTDIGAFTYINSKYGVKIDEYVQIGSHCSIYSVSTIDNKKGKVIINK
NARIGSHSVIMPNVEIGENSIIGAFSFVNKNIPPNTLAYGVPVKLIRKLTDEEIKKLLKEIE
>Mature_142_residues
MRFKNWKFPKIEHNKPTEYNWVVQYPENLELGKYTDIGAFTYINSKYGVKIDEYVQIGSHCSIYSVSTIDNKKGKVIINK
NARIGSHSVIMPNVEIGENSIIGAFSFVNKNIPPNTLAYGVPVKLIRKLTDEEIKKLLKEIE

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=136, Percent_Identity=35.2941176470588, Blast_Score=66, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 16264; Mature: 16264

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFKNWKFPKIEHNKPTEYNWVVQYPENLELGKYTDIGAFTYINSKYGVKIDEYVQIGSH
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHEECCCC
CSIYSVSTIDNKKGKVIINKNARIGSHSVIMPNVEIGENSIIGAFSFVNKNIPPNTLAYG
CCEEEEEECCCCCCEEEEECCCCCCCCEEEECCCEECCCCEEEEEEHHCCCCCCCCEEEC
VPVKLIRKLTDEEIKKLLKEIE
CHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MRFKNWKFPKIEHNKPTEYNWVVQYPENLELGKYTDIGAFTYINSKYGVKIDEYVQIGSH
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHEECCCC
CSIYSVSTIDNKKGKVIINKNARIGSHSVIMPNVEIGENSIIGAFSFVNKNIPPNTLAYG
CCEEEEEECCCCCCEEEEECCCCCCCCEEEECCCEECCCCEEEEEEHHCCCCCCCCEEEC
VPVKLIRKLTDEEIKKLLKEIE
CHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]